• Animal Bioscience
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• 제36권4호
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• pp.570-583
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• 2023

Objective: Fibroblast growth factors (FGFs) play critical roles in embryo development, and immune responses to infectious diseases. In this study, to investigate the roles of FGFs, we performed genome-wide identification, expression, and functional analyses of FGF family members in chickens. Methods: Chicken FGFs genes were identified and analyzed by using bioinformatics approach. Expression profiles and Hierarchical cluster analysis of the FGFs genes in different chicken tissues were obtained from the genome-wide RNA-seq. Results: A total of 20 FGF genes were identified in the chicken genome, which were classified into seven distinct groups (A-F) in the phylogenetic tree. Gene structure analysis revealed that members of the same clade had the same or similar exon-intron structure. Chromosome mapping suggested that FGF genes were widely dispersed across the chicken genome and were located on chromosomes 1, 4-6, 9-10, 13, 15, 28, and Z. In addition, the interactions among FGF proteins and between FGFs and mitogen-activated protein kinase (MAPK) proteins are limited, indicating that the remaining functions of FGF proteins should be further investigated in chickens. Kyoto encyclopedia of genes and genomes pathway analysis showed that FGF gene interacts with MAPK genes and are involved in stimulating signaling pathway and regulating immune responses. Furthermore, this study identified 15 differentially expressed genes (DEG) in 21 different growth stages during early chicken embryo development. RNA-sequencing data identified the DEG of FGFs on 1- and 3-days post infection in two indigenous Ri chicken lines infected with the highly pathogenic avian influenza virus H5N1 (HPAIV). Finally, all the genes examined through quantitative real-time polymerase chain reaction and RNA-Seq analyses showed similar responses to HPAIV infection in indigenous Ri chicken lines (R² = 0.92-0.95, p<0.01). Conclusion: This study provides significant insights into the potential functions of FGFs in chickens, including the regulation of MAPK signaling pathways and the immune response of chickens to HPAIV infections.

• Journal of Veterinary Science
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• 제24권1호
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• pp.13.1-13.11
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• 2023

Background: Highly pathogenic avian influenza viruses (HPAIVs) is an extremely contagious and high mortality rates in chickens resulting in substantial economic impact on the poultry sector. Therefore, it is necessary to elucidate the pathogenic mechanism of HPAIV for infection control. Objective: Gene set enrichment analysis (GSEA) can effectively avoid the limitations of subjective screening for differential gene expression. Therefore, we performed GSEA to compare HPAI-infected resistant and susceptible Ri chicken lines. Methods: The Ri chickens Mx(A)/BF2(B21) were chosen as resistant, and the chickens Mx(G)/BF2(B13) were selected as susceptible by genotyping the Mx and BF2 genes. The tracheal tissues of HPAIV H5N1 infected chickens were collected for RNA sequencing followed by GSEA analysis to define gene subsets to elucidate the sequencing results. Results: We identified four differentially expressed pathways, which were immune-related pathways with a total of 78 genes. The expression levels of cytokines (IL-1β, IL-6, IL-12), chemokines (CCL4 and CCL5), type interferons and their receptors (IFN-β, IFNAR1, IFNAR2, and IFNGR1), Jak-STAT signaling pathway genes (STAT1, STAT2, and JAK1), MHC class I and II and their co-stimulatory molecules (CD80, CD86, CD40, DMB2, BLB2, and B2M), and interferon stimulated genes (EIF2AK2 and EIF2AK1) in resistant chickens were higher than those in susceptible chickens. Conclusions: Resistant Ri chickens exhibit a stronger antiviral response to HPAIV H5N1 compared with susceptible chickens. Our findings provide insights into the immune responses of genetically disparate chickens against HPAIV.

• 한국지리정보학회지
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• 제23권4호
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• pp.140-155
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• 2020

본 연구는 가축질병이 밀집되어 발생했던 지역인 경기도 남부-충청도의 감염농가 입지 특성을 파악하여 가금농가의 질병 발생 확률을 분석하고 조건에 해당하는 지역을 도출하여 가축질병 발생예방과 차별적인 방역지역 선정 및 방역전략 설정의 근거와 보완대책의 기초자료로 사용하기 위해 수행되었다. 로지스틱 회귀분석 결과, 반경 3km내 가금농가 1개가 증가하면 HPAI에 감염될 확률이 전 단위에 비해 10.9% 증가한다. 2차선 이상 주요 도로와의 거리 1m가 증가하면 HPAI에 감염될 확률이 전 단위에 비해 0.001% 감소한다. 주요 철새도래지와의 15km 이내에 가금농가가 위치한 경우에서 15~30km로 변화하면 HPAI에 감염될 확률이 46.0% 감소한다. 주요 철새도래지와의 거리가 15km 이내에 가금농가가 위치한 경우에서 30km 이상으로 변화하면 HPAI에 감염될 확률이 88.5% 감소한다. 로지스틱 회귀분석 결과를 바탕으로 예측확률을 생성하고 도출된 입지요인인' 반경 3km내 가금농가 15개 초과, 주요 도로와의 거리 1km이내, 주요 철새도래지와의 거리 30 km이내'의 실제 지역을 도출하고 감염 비율을 측정하였다. 본 연구의 결과가 지역 내에서 가축질병이 발생할 확률이 높은 지역을 판별하여, 방역 주체가 대상 지역과 농가에 대해 선제적 방역을 실시하거나 차량을 통제하는 등의 차별적인 방역지역과 방역전략을 설정할 때, 그 근거와 보완대책 마련에 기초자료로 활용될 수 있을 것이라 기대한다.

• 한국동물위생학회지
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• 제41권2호
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• pp.119-123
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• 2018

There has recently been a growing demand for pathodiagnosis to determine the cause of death in cats. We retrospectively analyzed the diseases diagnosed in cats that were submitted to Animal and Plant Quarantine Agency (APQA) in 2015~2017. Overall diagnostic rate in feline samples was 85.2% (n=104/122). Among diagnosed cases, infectious diseases (n=63) were responsible for most of the feline diseases and feline panleukopenia (n=29) were most prevalent. Highly pathogenic avian influenza (HPAI) H5N6 was first diagnosed in cats at the end of December 2016 in the HPAI outbreaks. One case in 2015, 4 cases in 2016, and 14 cases in 2017 were associated with animal abuse, such as trauma and poisoning. These results suggest that suitable vaccination of feline infectious diseases, monitoring of the susceptible domestic animals during HPAI outbreaks, and interest on veterinary forensics to prevent and determine animal abuse are needed.

• Journal of Veterinary Science
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• 제25권2호
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• pp.20.1-20.15
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• 2024

Background: Avian influenza (AI) is a contagious disease that causes illness and death in poultry and humans. High pathogenicity AI (HPAI) H5N6 outbreaks commonly occur in Quang Ninh province bordering China. In June 2021, the first HPAI H5N8 outbreak occurred at a Quang Ninh chicken farm. Objectives: This study examined the risk factors associated with HPAI H5N6 and H5N8 outbreaks in Quang Ninh. Methods: A retrospective case-control study was conducted in Quang Ninh from Nov 2021 to Jan 2022. The cases were households with susceptible poultry with two or more clinical signs and tested positive by real-time reverse transcription polymerase chain reaction. The controls were households in the same village as the cases but did not show clinical symptoms of the disease. Logistic regression models were constructed to assess the risk factors associated with HPAI outbreaks at the household level. Results: There were 38 cases with H5N6 clade 2.3.4.4h viruses (n = 35) and H5N8 clade 2.3.4.4b viruses (n = 3). Compared to the 112 controls, raising poultry in uncovered or partially covered ponds (odds ratio [OR], 7.52; 95% confidence interval [CI], 1.44-39.27), poultry traders visiting the farm (OR, 8.66; 95% CI, 2.7-27.69), farms with 50-2,000 birds (OR, 3.00; 95% CI, 1.06-8-51), and farms with ≥ 2,000 birds (OR, 11.35; 95% CI, 3.07-41.94) were significantly associated with HPAI outbreaks. Conclusions: Combining biosecurity measures, such as restricting visitor entry and vaccination in farms with more than 50 birds, can enhance the control and prevention of HPAI in Quang Ninh and its spread across borders.

• 농촌계획
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• 제18권4호
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• pp.79-89
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• 2012

농가를 방문하는 가금관련업체의 관계자 및 차량은 HPAI 질병 확산의 매개체가 된다. 농가들의 가금관련업체 이용 정보를 이용하면 농가간의 연결을 확인할 수 있고 HPAI 확산 가중 네트워크를 구성할 수 있다. 네트워크 분석중 중심성 측정은 질병에 취약하거나 타 농가에 영향력이 큰 역할을 하는 농가를 분석하는 방법으로 HPAI 초기 확산을 통제하는 방법으로 이용된다. 단, HPAI 바이러스는 네트워크의 연결선 가중치에 따라서 확산 경로가 달라질 수 있다. 기존의 분석 방법은 확산 경로에 있어 대치되는 연결선의 강도와 연결선의 수 중 하나만을 고려하기 때문에 질병 확산을 정확히 모의하는데 한계가 있다. 그래서 본 연구에서는 2008년 발병한 한국 김제 지역의 39개 농가를 대상으로 가금관련업체 이용자료를 적용한 HPAI 확산 네트워크에 연결선의 가중치에 지수를 적용하는 방법으로 기존의 방법과 결과를 비교했다. 이 자료는 가금 산업 네트워크의 한국 지역 농가 적용성을 평가 할 수 있을뿐만 아니라 추후 잠재적인 질병 발병 차단을 위한 정보 제공에 중요한 역할을 할 것이다.

• 한국농공학회논문집
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• 제54권1호
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• pp.63-72
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• 2012

In livestock industry, damage caused by the epidemic diseases such as Foot-and-Mouth Disease (FMD), Highly-Pathogenic-Avian-Influenza (HPAI) and Porcine-Reproductive-and-Respiratory-Syndrome (PRRS) was very serious. The financial loss incurred from FMD alone which occurred on Nov. 2011 in Korea was estimated at 3 billion won, 23 % of annual livestock industry production. The livestock industry in Korea has greater risk of disease infection because of high density production, etc. Investigating the spread of livestock diseases should consider both direct and indirect contact as well as other various factors including airborne. Airborne infection of livestock disease was first hypothesised in the early 1900s, however, field experimental studies are still limited. Furthermore, no protocol is available in detecting airborne viruses in the field. In this study, effective virus samplers were investigated by comparative analysis of the type of samplers used detect to airborne virus. Laboratory experiments were conducted to compare virus samplers such as Bio-sampler, Dust-sampler, Compact-Cascade-Impactor (CCI) and Microflow in detecting PRRSV. Samples were analyzed by Reverse-Transcription PCR to assess the efficiency of the instrument in detecting the airborne virus. First, samples were classified into five levels according to light intensity of gel images and then the classified results were normalized. In every case, Bio-sampler and Dust-sampler were comparable with each other and have shown to be more effective than CCI and Microflow samplers.

• 농촌계획
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• 제19권4호
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• pp.137-147
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• 2013

Most of livestock houses are concentrated in certain area with mass rearing system resulting in rapid spread of infectious diseases such as HPAI (highly pathogenic avian influenza). The livestock-related vehicles which frequently travel between farms could be a major factor for disease spread by means of transmission of airborne aerosol including pathogens. This study was focused on the quantitative measurement of aerosol concentration by field experiment while vehicles were passing through the road. The TSP (total suspended particle) and PM10 (particle matter) were measured using air sampler with teflon filter installed downward the road with consideration of weather forecast and the direction of road. And aerosol spectrometer and video recorders were also used to measure the real-time distribution of aerosol concentration by its size. The results showed that PM2.5 was not considerable for transmission of airborne aerosol from the livestock-related vehicle. The mass generated from the road during the vehicle movement was measured and calculated to 241.4 ${\mu}g/m^3$ by means of the difference between TSP and PM2.5. The dispersion distance was predicted by 79.6 m from the trend curve.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2014년도 춘계학술대회
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• pp.295-298
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• 2014

2000년 이후 한국에서 기후변화(아열대성 및 잦은 이상기온) 및 농축산물 수입개방에 따른 국내 고병원성AI, 구제역, 가축질병 5회 발생으로 3조8천억원 피해와 기유입 확산되는 재선충의 10여년간 공적 방제비 2천383억원이 투입되었다. 또한 유입 가능성이 높은 흰개미 등의 외래식물해충으로 국가의 경제 사회적 피해와 영향이 증가되는 실정이다. 본 논문에서는 FTA확대에 따른 농식물로 인한 국가경제피해에 대한 대응 가능한 농업 농촌분야에 사물(IoT) 기반의 국가재난형 동 식물위성조기경보체계 운용전략에 대한 연구와 정책 수립을 연구한다. 또한 국가적 재난의 피해발생과 책임소재에 대한 정부의 피해보상 및 법 제도적인 책임 수행의 사전 증거자료 수집과 사후 보상 근거로서의 포렌식 기술이 포함된 정책이 적용되는 방법을 연구한다.

• 농촌계획
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• 제23권1호
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• pp.1-10
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• 2017

네트워크를 분석하는 방법인 stochastic 기반의 community bridge node finder는 네트워크 구조의 일부 정보만을 이용하여 중심지 및 확산 경로의 유추가 가능하다. 그러나, heterogeneity 네트워크에 적용하기 어려운 단점을 보유하고 있어, 본 연구에서는 이를 개선하고자 하였다. heterogeneity 네트워크에 적용할 수 있는 community bridge node finder 방법을 개발하기 위해 네트워크의 modularity를 계측하는 방법을 적용하였으며, 개선된 방법을 통해 community bridge node를 정량적으로 평가할 수 있어 heterogeneity 네트워크의 분석이 가능하였다. 농촌계획분야 정보의 경우, 대부분 자료의 불확실성이 존재하며 무결성이 떨어지는 특성을 가지고 있는 바 본 연구에서 적용한 전염병 확산 예측 뿐만 아니라 개선된 방법을 활용할 경우 주산지 정비 거점 분석 등 다양한 형태로 이용될 수 있을 것으로 기대된다.