• Title/Summary/Keyword: higher fungi

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Reduction of Microbial Populations on the Surface of Fresh Ginseng by Various Washing Treatments (세척처리에 따른 수삼 표면의 미생물 제어효과)

  • Kim, Hee-Su;Kim, Eun-Jeong;Choi, Jeong-Hee;Hong, Seok-In;Jeong, Moon-Cheol;Kim, Dong-Man
    • Food Science and Preservation
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    • v.17 no.3
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    • pp.405-409
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    • 2010
  • Surface cleaning is both essential and troublesome when a consumer seeks to eliminate soil attached to the surface of fresh ginseng because all ginseng purchased in the market is covered with soil, reflecting the post-harvest situation. To facilitate ginseng use at home, a fresh-cut type of ginseng is required. As a first step toward production of such ginseng, several washing and dipping treatments were investigated with respect to surface cleaning and reduction of microbial populations on fresh ginseng. In terms of microbial distribution on the surface of fresh ginseng, higher levels of viable bacteria (6.63 log CFU/each) and fungi (5.12 log CFU/each) were present on the rhizome head than on other regions of the root. Of the washing treatments tested, hand-brushing was effective for surface cleaning and to reduce microorganism levels on fresh ginseng, but use of a high-pressure water spray followed by hand-brushing was optimally effective. To further reduce the levelsof microorganisms on the surface of fresh ginseng after washing, additional dipping treatments in 70% (v/v) ethanol and electrolyzed acidic water (at pH 2.3) were somewhat effective but showed no significant differences compared with other dipping treatments tested, including a 3 ppm ozone solution, a 200 ppm sodium hypochlorite solution, or hot water at $50^{\circ}C$.

The comparative of Naringin and Chitosan using Natural preservation agents by LM and TEM (천연보존제 나린진과 키토산의 비교....LM & TEM적 소견)

  • Kim, In-Suk;Yoo, Geun-Chang;Chae, Soo-Chul;Lee, Chong-Bin;Jeon, Chang-Jin
    • Journal of Korean Ophthalmic Optics Society
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    • v.10 no.4
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    • pp.283-292
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    • 2005
  • This study investigated the effects of naringin, and chitosan in rabbits' corneas. Naringin, a glycone of naringenin, is a widely distributed bioflavonoid in the grapefruit and citrus peel, and it has already been reported as an antioxidant, antimicrobial, and anticancer agent. It has been used as a food preservatives and cosmetics. One of the natural preservatives, chitosan has also used in food preservatives, health drinks, and teas. Chitosan is distributed in the epithelium of crustacea, insects, and fungi. Naringin and chitosan have no harmful effects of cytotoxicity in the human body and they are recognized as an antibacterial for various forms of bacteria. The purpose of this study is to search for the ideal percentage of natural products to substitute the chemical preservatives occuring within the cornea and conjunctiva cytotoxicity and inflammations as wearing on soft contact lens. The present study compared the morphology of corneal epithelium and endothelium observed by light microscopy (LM) and transmission electron microscopy (TEM). In vivo methods, We investigated the effects of natural preservatives on soft contact lens. We inserted 3-4 drops of the naringin and chitosan, directly on rabbits' corneas 4 times per a day during one week. After enucleation of cornea, morphorgical damages of the epithelium and endothelium were observed by LM and TEM. In view of ultrastructure, chitosan caused siginficant damage on the epithelium and endothelium of cornea. The damage of cells was higher in chitosan treated cornea than 0.01, 0.1, and 1% of naringin. The 1% of naringin also expressed cell damage seriously. The results suggest that the most important thing is to use the reasonable percentage of preservatives for contact lens solutions.

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Investigation of Colony Forming Unit (CFU) of Microorganisms in the Paprika-grown Greenhouses Using Open and Closed Soilless Culture Systems (순환식과 비순환식 수경재배 방식에 따른 파프리카 재배온실 내 미생물의 집락형성단위(CFU) 조사)

  • Ahn, Tae In;Kim, Do Yeon;Son, Jung Eek
    • Horticultural Science & Technology
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    • v.32 no.1
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    • pp.46-52
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    • 2014
  • This study was conducted to compare colony forming unit (CFU) of microorganisms in closed and open soilless culture systems for estimating the possibility for potential disease occurrence. Samples were collected at four different positions in four commercial greenhouses with closed or open soilless culture system using rock wool or coir as substrate, respectively. The distance between sampling positions was 3 cm starting from the substrate and the surface area of each position was $25cm^2$. The CFU of fungi was significantly higher in the open system, while that of bacteria was not significantly different but showed relatively lower in the closed system. Samples collected at the plastic surface of the substrates where little environmental effects occurred from drainage showed lower CFU than any other positions. The principal component analysis showed that samples collected on the drainage pathway highly affected the changes in microbial population in the greenhouse. These results indicated that greenhouses with closed soilless culture are expected to have more advantageous conditions for restraining the microbial growth, resulting in the lower potential of disease occurence in greenhouse ecosystem.

Effects of Arbuscular Mycorrhiza Inoculation and Phosphorus Application on Early Growth of Hot Pepper(Capsicum annum L.) (Arbuscular mycorrhiza의 접종방법 및 인산시용량이 고추(Capsicum annum L.)의 초기생장에 미치는 영향)

  • Park, Hyang-Mee;Kang, Hang-Won;Kang, Ui-Gum;Park, Kyeong-Bae;Lee, Sang-Sun;Song, Sung-Dahl
    • Korean Journal of Soil Science and Fertilizer
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    • v.32 no.1
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    • pp.68-75
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    • 1999
  • This study was conducted to examine the effects of Arbuscular Mycorrhizae inoculation and phosphorus application on early growth of hot pepper. Gigaspora margarita and Acaulospora spinosa were chosen for this investigation and inoculated into soils of different P levels by varying inoculation time and density. After treatment, some relevant growth responses of hot pepper were measured. Regardless of soil P levels, hot peppers treated with arbuscular mycorrhizal fungi had 5~34% more fresh weight than those untreated, but the effect of inoculation time and density was not different between two species. With decreased P levels, the infection rate and dependency of hot peppers increased. The content of P and K of AMF-inoculated hot peppers increased with increasing P levels, but the shoot to root ratio of those elements decreased. The results of this study showed that inoculation of AMF would be effective in promoting growth of hot pepper seedlings and increase transplant adaptation due in part to the resulted higher root development.

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Evaluation of Physico-mechanical Properties and Durability of Larix kaempferi Wood Heat-treated by Superheated Steam (과열증기 열처리 낙엽송재의 물리·역학적 성능 및 내후성능 평가)

  • Park, Yonggun;Park, Jun-Ho;Yang, Sang-Yun;Chung, Hyunwoo;Kim, Hyunbin;Han, Yeonjung;Chang, Yoon-Seong;Kim, Kyoungjung;Yeo, Hwanmyeong
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.5
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    • pp.776-784
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    • 2016
  • In this study, green Larix kaempferi lumber was heat-treated by using superheated steam (SHS) at a pilot scale and then various physico-mechanical properties of the heat-treated wood were evaluated and compared with the properties of conventional hot air (HA) heat-treated wood. Decay resistance of brown rot fungi and compressive strength parallel to the grain of the SHS heat-treated wood without occurrence of drying check from green lumber were increased. On the other hand, density, equilibrium moisture content, shrinkage, and bending strength of the SHS heat-treated wood were lower than those of the conventional HA heat-treated wood. Because heat transfer and thermal hydrolysis of SHS heat treatment was accelerated by a large amount of water, the effect of SHS heat treatment on the physico-mechanical properties was higher than that of HA heat treatment at the similar conditions of temperature and time. From the results of this study, because green lumber can be heat-treated without occurrence of cracks or checks by using SHS and similar heat treatment effect on the physico-mechanical properties of wood can be produced despite a low temperature or short time of heat treatment, it is expected that heat time and energy consumption could be reduced by using SHS.

Substituent Effect on the Fungicidal Activity of New N-substituted Benzotriazol-1-yl Derivatives (새로운 N-치환 benzotriazol-1-yl유도체의 항균활성에 미치는 치환기 효과)

  • Yu, Seong-Jae;Sung, Min-Gyu;Kim, Dae-Whang;Sung, Nack-Do
    • Applied Biological Chemistry
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    • v.40 no.1
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    • pp.80-84
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    • 1997
  • Series of new chiral N-substituted benzotriazol-1-yl derivatives were synthesized and their fungicidal activities in vitro against gray mold(Botrytis cinerea), black spot(Alternaria kikuchiana) and phytophthora blight(Phytophthora capsici) were measured by the agar medium dilution method. The substituents effects between the fungicidal activities (obs. $pI_{50}$) and a various physicochemical parameters of phenoxy or thiophenoxy group(X) & alkyl or phenyl group(Y) were analyzed by the multiple regression technique. From the analyzed substituent effects, the structure-activity relationship(SAR) equations shows that the antifungal activities depend on the parameters for the optimal molecular hydrophobicity($({\Sigma}logP)_{opt}$), Van der Waals (${\Sigma}Vw$>0) volume(${\AA}^3$) and inductive constant with electron withdrawing group(${\sigma}_I$,Y>0). The activity in affected by the inductive effect (${\sigma}_I$,Y>${\sigma}_g$X) of Y-group rather than the X-group. The phenoxy substituents, 1, showed higher antifungal activity tn the thiophenoxy substituents, 2. For 1, polar substituent constant(${\sigma}^*$) was an important factor in determining the activity. And the tribromomethyl substituent, 1g showed the highest activity against the tee fungi.

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Effects of UV Sterilization on Quality of Acer mono Sap (자외선 살균이 고로쇠 수액의 품질에 미치는 영향)

  • Choe, Su Bin;Han, Sang Jin;Han, Ouk Hee;Kim, Hak Su;Kang, Sung Tae
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.1
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    • pp.148-152
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    • 2013
  • An ultraviolet (UV) sterilization system was developed to decrease the number of microorganisms in filtered Acer mono sap (FAS). The Acer mono sap (AS) was passed through 18 strips of PTA fluoroplastic tubing with 30 UV lamps (total 1,170 W). During passage, the AS was effectively exposed to the UV rays. The total bacteria, coliform group and fungi were sterilized at a flow rate ranging from 852 to 1,358 mL/min and a UV power higher than 156 W. Although the sensory score of the UV-sterilized AS was significantly lower than that of the untreated AS, the sensory score was better under the condition of 390 W and 852~1,358 mL/min than under the other conditions examined. ANOVA did not reveal a significant difference in pH, total acidity, sugar content and color characteristic under all conditions tested (p<0.05). Considering the overall attributes of the AS quality, the optimum sterilization condition was determined to be 390 W and 852~1,358 mL/min.

Effects of High Pressure Treatment on Antioxidant Compounds and Activity of Germinated Rough Rice (Oryza sativa L.) (고압처리가 발아벼의 항산화 성분과 활성에 미치는 영향)

  • Kim, Min Young;Lee, Sang Hoon;Jang, Gwi Young;Park, Hye Jin;Meishan, Li;Kim, Shinje;Lee, Youn Ri;Lee, Junsoo;Jeong, Heon Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.11
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    • pp.1783-1791
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    • 2013
  • This study is investigated to evaluate the enhancement of antioxidant compound and activity of rough rice with different germination periods and high pressure treatment. The subject was germinated at $37^{\circ}C$ for 6 days (HP0), and then the germinated rough rice were subjected to 30 MPa for 24 hr (HP24) and 48 hr (HP48), respectively. HP0, HP24 and HP48 samples were prepared and extracted with 80% ethanol. The highest total polyphenol contents (5.15 mg/g) occurred in treating at HP48 after germination for 5 days. The total phenolic acid contents including gallic acid, chlorogenic acid, catechin, ${\rho}$-coumaric acid, ferulic acid, salicylic acid, naringin, myricetin, trans-cinnamic acid, naringenin and kaempferol increased from $37.26{\sim}204.32{\mu}g/g$ at HP0 to $77.29{\sim}283.05{\mu}g/g$ at HP48. In antioxidant activity analyses, HP48 extracts showed higher values in ABTS and DPPH radical scavenging activity, reducing power, and $Fe^{2+}$ iron chelating effect than those of the HP24 and HP0 extracts. These results suggest that the combined treatment of high pressure treatment and germination process efficiently enhanced antioxidant compound and activity of rough rice.

Inoculation Effect of Quercus acutissima Carr. with Pisolithus tintorius (Pers.) Coker et Couth and Rhizopogon rubescens(Tul.) Tul (상수리나무에 대(對)한 모래밭버섯균(菌)과 알버섯균(菌)의 접종효과(接種效果))

  • Lee, Chun-Yong;Lee, Won-Kyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.20 no.1
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    • pp.85-88
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    • 1987
  • Quercus acutissima seedlings were grown for two years in fumigated or non-fumigated in nursery soil in a medium with and without vegetative inoculum of the ectomycorrhizal fungi, Pisolitlius tinctorius (Pt) and Rhizopogon rubescens (Rr). Mycorrhizal formation were 42% in fumigated Pt inoculation and 36% in fumigated Rr inoculation. $2.16{\ell}\;per\;m^2$ of Pt vegetative inoculum in fumigated soil stimulated the seedling height (98%), root collar diameter (132%) and weight (420%). And Rr inoculation in fumigated soil increased the seedling height (44%), root collar diameter (23%) and weight (157%) compared with non-treated plot. There was no effect of Pt and Rr inoculation on the growth in non-fumigated soil. Nitrogen and $P_2O_5$ contents in foliage were not different by the treatment but $K_2O$ and Ca in fumigated soil were higher than non-fumigated soil.

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Studies on Microflora of the Paddy and Upland Soils of Korea -I. Distribution of Microflora of the Paddy Soils (우리나라 논, 밭토양의 미생물상(微生物相)에 한 연구 -I. 논토양의 미생물(微生物) 분포조사(分布調査))

  • Yoo, Ick-Dong;Yun, Seh-Young;Lee, Myong-Goo;Rhu, Jin-Chang
    • Korean Journal of Soil Science and Fertilizer
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    • v.16 no.2
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    • pp.195-202
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    • 1983
  • Sixty paddy soil samples were collected from the different Korean agricultural climatic area to find relationship between soil physicochemical properties and soil microorganisms. The results are summarized as follows : 1. The mean numbers of microorganisms in collected paddy soils were $121.8{\times}10^5$ in bacteria (B), $22.5{\times}10^5$ in actinomycetes (A) and $32.4{\times}10^3$ in fungi (F) per gr soil. The ratios of B/F, B/A, and A/F were 385, 5.1 and 82, respectively. 2. Number of soil microorganism was lowest in Mountainous area, lower in Eastern Coast, Gyeonggi Bay and Chungcheong Continental area, but higher in Honam Plain and Southern Part. 3. The significant positive correlation were obtained between the number of microorganisms and soil chemical properties, available phosphorous, $K^+$, $Mg^{++}$, T-C and soil pH. 4. The number of soil microorganism was in the order of Clay loamy soil > Clayey soil > Loamy soil > Sandy loamy soil.

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