• 한국약용작물학회지
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• 제17권6호
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• pp.407-411
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• 2009

Hepatoprotective and antioxidant activities of Acer mono and A. okamotoanum were compared after being extracted by low temperature and high pressure (LTHP) at 20 MPa and $60^{\circ}C$ for 15 minutes. Extraction yield of both A. mono and A. okamotoanum was increased about 40~43% by this extraction process. On scavenging activities, the bark of A. okamotoanum from this extraction process showed the highest activity as about 97%. This value was higher than that from conventional water extraction and A. mono extracts. Both of A. mono and A. okamotoanum showed high ability on nitrite scavenging, but decreasing tendency according to increasing of pH. On SOD-like test, A. okamotoanum had the highest activity as 46.28% at $1.0\;mg/m{\ell}$ concentration. A. okamotoanum extracted by LTHP also showed the highest activity as 197.38% in adding $1.0\;mg/m{\ell}$ concentration. Generally, the extracts from low temperature and high pressure extraction process are higher hepatoprotective and antioxidant activities than that from conventional water extraction. It can conclude that the bark of A. okamotoanum has better biological activities than other parts of A. mono.

• Food Science and Biotechnology
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• 제14권6호
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• pp.860-865
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• 2005

A gene for a putative glucoamylase, stg, of a hyperthermophilic archae on Sulfolobus tokodaii was cloned and expressed in Escherichia coli. The recombinant glucoamylase (STGA) had an optimal temperature of $80^{\circ}C$ and was extremely thermostable with a D-value of 17 hr. The pH optimum of the enzyme was 4.5. Being different from fungal glucoamylases, STGA hydrolyzed maltotriose (G3) most efficiently. Gel permeation chromatography and sedimentation equilibrium analytical ultracentrifugation analysis showed that the enzyme existed as a dimer. STGA was stable enough to hydrolyze liquefied com starch to glucose in 4 hr at $90^{\circ}C$ with a yield of95%. Comparison of the $k_{cat}$ values for the hydrolysis and the reverse reaction at $75^{\circ}C$ and $90^{\circ}C$ indicated that glucose production by STGA was more efficient at $90^{\circ}C$ than $75^{\circ}C$. Therefore, STGA showed great potential for application to the industrial glucose production process due to its high thermostability.

• Ocean and Polar Research
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• 제43권3호
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• pp.149-164
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• 2021

Since the existing mass production and consumption systems are no longer sustainable, countries are pushing for policies to make fisheries by-products as resources in an eco-friendly manner, and international standards are also being strengthened to increase the value of by-products. In Korea, economic and environmental perceptions of the by-products are rapidly changing, such as realizing carbon neutrality and enhancing circular resources by Korean Sustainable Development Goals. Raw materials derived from the by-products have been steadily imported from 2018. In particular, the number of imports of fish collagen peptides was only 16 number of times in 2017, but was rapidly increased to 483 number of times in 2020. Simultaneously, the demand for raw materials and nutrients for health functional food derived from fish by-products, which did not exist statistically until 2017, started to arise from 2018, and in 2019, consumption of high-value-added raw materials for fish by-products increased by 45% compared to the previous year. However, limitations are in legal and biotechnical industry aspects while its value as a biomaterial is recognized in the by-products-related industry. In this study, therefore, the status of by-products for upcycling biomaterials was reported and provided a scientific basis for supporting governmental strategies. In order to fulfill with the principles of a sustainable circular economy, the factors on hinder the marine bio-industrialization of the by-products were derived and suggested directions and plans for development into a high-value added the by-products as the marine bio-industry by substituting imported raw materials to support the development.

• Food Science and Biotechnology
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• 제17권2호
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• pp.234-240
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• 2008

The optimal conditions for the production of branched dextrin from com starch (CSBD) using branching enzyme (BE) were established by investigating the degree of retrogradation of the gelatinized starch. The physicochemical properties of CSBD prepared using the established process were evaluated. It was found that physicochemical properties of com starch were greatly modified by BE treatment. CSBD had a higher dextrose-equivalent value and water solubility than the corresponding control. On the other hand, the viscosities in gelatinized solution and amylose contents of CSBD were lower than those of the control. A high-performance size-exclusion chromatography/multiangle laser light scattering/retractive index (HPSEC/MALLS/RI) system showed that the average molecular weight of CSBD was lower than that of the control. The pasting viscosities of CSBD were stable during the entire temperature cycle. In general, the BE treatment resulted in the retrogradation during storage being lower for CSBD than for the control.

• KSBB Journal
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• 제25권4호
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• pp.401-407
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• 2010

We established a strategy for bioethanol production using the hydrolysate of rape stem, in which the inhibitor cocktail was added intentionally. The final goal of this study was to circumvent the detoxification process when the hydrolysate of lignocelluloisic biomass contained the toxic substances in high concentration. When six yeast strains were examined, Sacchromyces cerevisiae ATCC 96581 and Pichia stipitis CBS 7126 were relatively resistant to inhibitor cocktail. Then, using strains 96581 and 7126, we designed a process strategy for bioethanol production, assuming that the concentration of toxic substance in the hydrolysate of rape stem was remarkably high. When strains 96581 and 7126 were inoculated simultaneously, it was observed that strain 7126 produced bioethanol as well as strain 96581, although the concentration of inhibitor cocktail was 18.2% (v/v). Finally, throughout this co-cultivation of strains 96581 and 7126, bioethanol was produced about 6.0 (g/L), and bioethanol yield reached at 0.4 (g-bioethanol/g-reducing sugar) (78.4% of theoretical value).

• 한국식품저장유통학회지
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• 제24권2호
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• pp.181-186
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• 2017

본 연구에서는 토마토를 일정한 기간(15일) 동안 저장하여 토마토의 품질인자인 감모율, 색상과 경도를 분석하고 비 파괴적 방법으로 토마토 경도를 측정한 결과로 경도와 감모율 사이의 상관관계를 분석하고 선형회귀모델을 도출하여 토마토 품질을 예측하고자 하였다. 그린하우스에서 재배된 '티와이250'품종을 수확한 후 일반 과실 종이 박스에 포장한 후 설정환경이 $10^{\circ}C$, 90%RH인 항온항습챔버에 저장하여 3일 간격으로 경도, 무게와 색상 변화를 조사하였다. 15일간 저장 중 감모율은 저장기간이 증가함에 따라 증가하나 1.1% 정도에 머물러 단순히 감모율 인자로만 판단했을 때 토마토의 신선도 품질에 영향 끼치는 수준은 아니다. 색상변화 중 명도와 색조 각은 저장기간의 증가에 따라 감소하는 경향을, a/b와 ${\Delta}E$는 증가하는 경향을 나타냈었고 일원분산분석결과를 볼 때 유의한 수준이었다(p<0.001). 경도는 저장기간이 증가함에 따라 감소하는 경향을 나타냈으며 저장 15일차에는 경도감소율이 40% 이상인 것으로 나타났다. 그리고 비 파괴방법으로 측정한 경도 값과 감모율 사이의 상관관계를 분석하여 선형회귀모델 $WL=-0.0241{\times}F+1.5213$을 하였으며 모델의 추정치 오차는 ${\pm}0.231$이었다. 이러한 결과에 비추어볼 때 수확 후 일정한 저장환경에서 비파괴적인 경도 측정을 통해 감모율을 추정하고 신선도를 판단하는 지표고 사용하는 것이 가능한 것으로 판단되었다.

• Journal of the Korean Wood Science and Technology
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• 제42권2호
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• pp.183-192
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• 2014

For the structure determination of D-(+)-sucrose, which consists of ${\alpha}$-D-(+)-glucose and ${\beta}$-D-(+)-fructose, it was acetylated with acetic anhydride and triethyl amine, pyridine, zinc chloride, and sodium acetate as catalysts. The acetylated D-(+)-sucrose was acid-hydrolyzed using sulfuric acid and sodium chloride in methanolic solution. The structures of the reaction products were determined by $^1H$-NMR and $^{13}C$-NMR spectra. The yield of the acetylation indicated the high value in zinc chloride as 70% in zinc chloride catalyst. The acid-hydrolyzed product of the acetylated D-(+)-sucrose, 2,3,4,6,1',3',4',6'-octa-O-acetyl-D-(+)-sucrose, gave 2,3,4,6-tetra-O-acetyl-${\beta}$-D-(+)-glucose and it suggests that the acetylated D-(+)-sucrose was rearranged through the formation of oxonium ion by mutarotation in the 2,3,4,6-tetra-O-acetyl-${\alpha}$-D-(+)-glucose moiety and through the ring opening in the 1',3',4',6'-tetra-O-acetyl-${\beta}$-D-(+)-fructose moiety.

• 대한금속재료학회지
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• 제50권5호
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• pp.395-400
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• 2012

In this study, we have investigated the surface morphology of hydroxyapatite (HA) coated Ti alloy surface using pulsed laser plating. The HA (tooth ash) films were grown by pulsed KrF excimer laser, film surfaces were analyzed for topology, chemical composition, crystal structure and electrochemical behavior. The Ti-6Al-4V alloy showed ${\alpha}$ and ${\beta}$ phase, Cp-Ti showed ${\alpha}$ phase and the HA coated surface showed HA and Ti alloy peaks. The HA coating layer was formed with $1-2{\mu}m$ droplets and grain-like particles, particles which were smaller than the HA target particle, and the composition of the HA coatings were composed of Ca and P. From the electrochemical test, the pitting potential (1580 mV) of HA coated Ti-6Al-4V alloy was higher than those of Cp-Ti (1060 mV) and HA coated Cp-Ti (1350 mV). The HA coated samples showed a lower current density than non-HA coated samples, whereas, the polarization resistance of HA coated samples showed a high value compared to non-HA coated samples.

• Journal of the Korean Wood Science and Technology
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• 제51권6호
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• pp.447-447
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• 2023

This study aimed to convert underutilized moso bamboo into high-value added products such as fences, interior materials, lighting fixtures, and accessories. Green moso bamboo poles with a diameter of approximately 10 cm and a length of approximately 3.7 m were heat treated at 140℃ using a large-scale kiln. The processing time was meticulously adjusted through various stages, including pretreatment (6-8 hours at 60℃), cooking (8-10 hours at 100℃), steaming (26-30 hours at 120℃), heating (4-6 hours at 140℃), and finally, cooling (below 80℃). A meticulously designed heat treatment process has enabled efficient mass production of moso bamboo poles with improved qualities, including minimal splitting, moisture levels below 3%, and a specific gravity of 1.05. The focus of this study was to present the physical and drying properties, such as color, dimensional change, specific gravity, moisture content, and splitting, observed during the heat treatment process.

• 한국재료학회지
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• 제15권2호
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• pp.134-138
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• 2005

This study was performed to investigate the surface properties of electrochemically oxidized pure niobium by anodic oxide and hydrothermal treatment technique. Niobium specimens of $10mm\times10mm\times1.0mm$ in dimension were polished sequentially from $\#600,\;\#800,\;\#1000$ emery paper. The surface of pure niobium sperimens was anodized in an electrolytic solution that was dissolved calcium and phosphate in water. The electrolytic voltage was set in the range of 250 V and the current density was $10mA/cm^2$. The specimen was hydrothermal treated in high-pressure steam at $300^{\circ}C$ for 2 hours using an autoclave. And all specimens were immersed in the in the Hanks' solution nth pH 7.4 at $37^{\circ}C$ for 30 days. The surface of specimen was characterized by surface roughness, scanning electron microscope(SEM), energy dispersion X-ray analysis(EDX), X-ray photoemission spectroscopy(XPS) test. The value of surface roughness was the highest in the anodized sample and $0.41{\pm}0.04\;{\mu}m$. The results of the SEM observation show that oxide layers of the multi porosity in the anodized sample were piled up on another, and hydroxyapatite crystal was precipitate from the surface of the hydrothermal treated sample. In the XPS analysis, O, Nb, C peak and small amounts of N peak were found in the polished specimens while Ca and P peak in addition to O, Nb, C and peak were observed in the hydrothermal treated sample.