• Bulletin of the Korean Chemical Society
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• v.12 no.5
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• pp.504-509
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• 1991

S. fradiae showed very high activity of isocitrate dehydrogenase compared to other microorganisms. The activity of this enzyme was increased with the growth of the organism. But the increase might not imply its involvement in the growth. Rather its increased activity seemed to have a connection with the biosynthesis of neomycin. The enzyme showed high specificity toward $NADP^+$ and D-isocitrate with Km values of 5.75 and 6.74 uM, respectively, It was activated by $Mn^{2+}$. Its molecular weight was estimated from its gel retardation coefficient to be in the range of 61,000-63,000 daltons and its optimum pH was 8.0. The enzyme was thermally unstable.

• Archives of Pharmacal Research
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• v.5 no.2
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• pp.87-91
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• 1982

In order to develop an analytical method for amikacin and butirosin in presence of their parent antibiotics, kanamycin A and ribostamycin, high-performance liquid chromatographic technique and microbioassay method were evaluated and compared. Using high performance liquid chromatography, two acylated antibiotics, amikacin and butirosin was partially separated from their parent antibiotics, to provide a qualitative analytical method. In microbioassay using Pseudomonas aeruginosa TI-13, a producer of aminoglycoside-3-phosphotransferase I, only acylated antibiotics were selectively analyzed when paper disc-susceptibility assay was used. The standard curve showed a good correlation between the response and odse in semilogarithmic plat with correlation coefficients above 0.96, and analytical deviation from expected dose was within 10%.

• Journal of Practical Agriculture & Fisheries Research
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• v.18 no.1
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• pp.47-61
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• 2016

Ranging from a variety that a consumer to purchase and consume, and a variety with high quality and pest resistance, sustaining biological diversity as well as hiring global experts who understand breeding policy, it tightly maintains relationship with producer, consumer, and exporter to promote superior species for customized export to exporting country. By carrying out researches on cost saving and how to shorten the period of apple to cultivate, it consolidates its stance as a competent apple exporting country of apple and pear with high competetiveness. In order to compete against apple and pear of New Zealand, farmers argue that they need budget and researchers also argue that "No money, no research." Due to certain limit of governmental budget support, it is believed that it is time to fund our own way to promote apple research center. To come up with the best apple production, all of those who associates with apple such as farmers, researchers, government, exporters, and consumer should do their best to enhance the apple and pear industry.

• Journal of Advanced Marine Engineering and Technology
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• v.34 no.6
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• pp.792-797
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• 2010

Microwave oven and household cooker are devices of high voltage producer and high voltage storage batteries respectively for formation of necessary high frequencies at drive. These devices emit much heat energy because they are run at high voltages. Therefore, emitted heat energy becomes a factor that raises temperature of microwave ovens' main frame. In this research, the analysis shows the temperature distribution in microwave oven with the cooling fan drive conditions and the heat energy occurrence conditions. According to the analysis, as the speed of air outpoured in cooling fan increases, and the internal temperature decreases quantitatively. Also the inside temperature distribution was investigated by controlling heat energy emission.

• Proceedings of the KIEE Conference
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• 2005.04a
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• pp.134-139
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• 2005

This paper presents a cost effective quasi-resonant soft-switching PWM high frequency inverter with minimum circuit components. This inverter can achieve wider soft commutation, simpler power circuit configuration, smaller volumetric size, lower cost and wider power regulation range, higher-efficiency as compared with single ended quasi-resonant ZVS-PFM inverter and active voltage clamped quasi-resonant ZVS-PWM inverter. The operation principle of the proposed inverter is described on the basis of the simulation and experimental results, together with its operating performances in steady state. The operating performances of this unique proposed high frequency inverter based on ZVS and ZCS arms-related soft commutation principle is evaluated and discussed as compared with the active voltage-clamped ZVS-PWM inverter and a conventional single-ended ZVS-PFM inverter. The practical effectiveness of a novel type quasi-resonant soft-switching PWM high frequency inverter using IGBT is actually proved for consumer induction heated appliances as rice cooker, hot water producer, steamer and super heated steamer. The extended bidirectional circuit topology of quasi-resonant PWM high frequency inverter with minimum circuit components is demonstrated, which operate as the direct frequency changer.

• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.2019-2028
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• 2018

Natural astaxanthin mainly derives from a microalgae producer, Haematococcus pluvialis. The induction of nitrogen starvation and high light intensity is particularly significant for boosting astaxanthin production. However, the different responses to light intensity and nitrogen starvation needed to be analyzed for biomass growth and astaxanthin accumulation. The results showed that the highest level of astaxanthin production was achieved in nitrogen starvation, and was 1.64 times higher than the control group at 11 days. With regard to the optimization of light intensity utilization, it was at $200{\mu}mo/m^2/s$ under nitrogen starvation that the highest astaxanthin productivity per light intensity was achieved. In addition, both high light intensity and a nitrogen source had significant effects on multiple indicators. For example, high light intensity had a greater significant effect than a nitrogen source on biomass dry weight, astaxanthin yield and astaxanthin productivity; in contrast, nitrogen starvation was more beneficial for enhancing astaxanthin content per dry weight biomass. The data indicate that high light intensity synergizes with nitrogen starvation to stimulate the biosynthesis of astaxanthin.

• Cartoon and Animation Studies
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• s.38
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• pp.29-51
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• 2015

With the progress in high speed internet networks, mobile devices and social networking, the eco-system of the media has shifted from that where the flow of content was one-way from the producer to the consumer. A so-called 'prosumer' culture has taken root where the consumer himself produces media content. Along with these trends, various video-sharing platforms such as youtube has a method of allocating advertisement profit to the content producer, offering a win-win platform for content pro-sumers. This allows the channels to attract several tens of millions of subscribers and raise an annual income of over 10 billion Won, marking a revolutionary change in the content industry. This paper seeks to analyze video distribution channels and short-form media content that are showing continuous growth to identify new markets where animated content can make progress in an era of online video media platforms, as well as provide a future direction for small teams of creators of animated films to survive and thrive in this environment.

• Journal of the Korean earth science society
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• v.29 no.1
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• pp.13-28
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• 2008

About 450 specimens of Lockeia were discovered from the lacustrine siltstone of the Early Cretaceous Jinju Formation of Jin Island, southern coast of Korea. They are very elongated, seed-shaped Lockeia characterized by a large size, mostly 60-70 mm long and 15-20 mm wide. They are characteristically sharp longitudinal furrow bounded by steeply inclined both margins, elevated marginal rims and sharp pointed both longitudinal furrow ends. This trace fossil is herein described as Lockeia gigantus ichnosp. nov. Plicatounio, a freshwater bivalve which does not occur occurs occasionally within Lockeia gigantus is regarded as the most-likely producer of this resting trace fossil. This new trace fossil represents the largest Lockeia ever known and the first record of Lockeia from the Cretaceous non-marine deposits in the world. This fossil also represents an unusual example of resting trace fossil (Lockeia) associated with a possible producer (bivalve Plicatounio) lived in community in the shallow lacustrine environment.

• Journal of Microbiology and Biotechnology
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• v.15 no.1
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• pp.59-65
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• 2005

Poly-${\gamma}$-glutamic acid (PGA) is an unusual anionic polypeptide and has great potential as an environmentally and industrially significant biodegradable material. A new ${\gamma}$-PGA producer, Bacillus mesentericus MJM1, with high production capacity was isolated from Korean domestic Chungkuckjang bean paste. It produced ${\gamma}$-PGA at the level of 10 g/l in suitable media. The viscosities of 5% initially extracted mucin and purified ${\gamma}$-PGA solutions were 660 cps and 600 cps, respectively. The produced ${\gamma}$-PGA polymer consisted of 2,000 glutamic acid residues with even proportion of L and D types with molecular mass of about 200- 300 kDa. Bacillus mesentericus MJM1 displayed ${\gamma}$-glutamyltranspeptidase (${\gamma}$-GTP) activity that is known to play a key role in ${\gamma}$-PGA biosynthesis. The ${\gamma}$-GTP coding region was located on the plasmid of 5.8 kb. The plasmid, named pMMH1, is a rolling-circle replication (RCR) plasmid and additionally contained a replication origin and type I signal peptidase (sipP) coding region.

• Journal of Microbiology and Biotechnology
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• v.19 no.11
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• pp.1369-1373
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• 2009

Succinic acid was produced by continuous fermentation of Actinobacillus succinogenes sp. 130Z in an external membrane cell recycle reactor to improve viable cell concentration and productivity. Using this system, cell concentration increased to 16.4 g/l at the dilution rate $0.2\;h^{-1}$, up to 3 times higher than that of batch culture, and the volumetric productivity of succinic acid increased up to 6.63 g/l/h at the dilution rate $0.5\;h^{-1}$, 5 times higher than that of batch fermentation. However, in the continuous culture using a high dilution rate, operational problems including severe membrane fouling and contamination by lactic acid producer were observed. Another succinic acid producer, Mannheimia succiniciproducens MBEL55E, was also utilized in this system, and the cell concentration and productivity of succinic acid at the dilution rate of $0.3\;h^{-1}$ were found to be above 3 and 2.3 times higher, respectively, compared with those obtained at the dilution rate of $0.1\;h^{-1}$. These observations give a deep insight into the process design for a continuous succinic acid production by microorganisms.