• 농촌지도와개발
• /
• 제9권2호
• /
• pp.263-277
• /
• 2002

The objective of this study was to compare the farm efficiency of members of cooperative firms with that of non-members in Korea. Data were collected from 27 farms members of three cooperatives and 13 non-member farms, based on the managerial achievements of 1999. For the analysis, the data were divided into three different farming types: type I and II of member farms and non-member farms. Economic analysis were implemented using DEA(Data Envelopment Analysis). Major findings was as follows. Total average technology efficiency was 0.782, which implies that there still remains 21.8% of improvement possibility. Among the three types, type I showed the highest technology efficiency of 0.9055, while type II and non-member farms showed 0.7670 and 0.7171, respectively. This means that these farms have relatively high potential improved. Also, comparing technology efficiency by dividing into pure technology and scale efficiency, all type of the farms exhibit a higher pure technology efficiency compared to scale efficiency. Therefore, to improve current technology efficiency, farms need to focus more on pure technology efficiency improvement. Meanwhile, profit analysis based on farm size indicate that most of the farms demonstrated the increasing-return-to-scale, which means it is necessary to extend the farm size to improve technical efficiency. Type I member farms achieve higher profit and efficiency than other two types and relatively higher producer prices through introducing cooperative sorting, grading and packing. Also, these labor saving technologies increased farm size.

• 지역사회간호학회지
• /
• 제10권2호
• /
• pp.508-524
• /
• 1999

We would like to identify the current status and the problem of the material for sexual education in Korea by analyzing the contents and the subjects of the materials. The 274 materials were collected from 80 institutes from March 1999 to June 1999. The collected ones were analyzed according to title. year of production. producer. subjects of materials and contents. Also, the materials in the internet was searched. Result were summarized by the descriptive statistics with SPSS/PC as followings: Type of materials were video tape (155), books (70), projection slides (43) and CD-ROM (6); Number of production from each institute were 54 from Planned Parenthood Federation of Korea, 39 from Korea Broadcasting System. 23 from Seoul School Health Center. 22 from Seoul Education and Science Research Institute, 16 from Korea Sexual Violence Relief Center, 13 from Republic of Korean National Redcross. and 10 from Korea Educational Development Institute; Subjects of the materials were middle and high school children (55), teachers (35), primary schoolchildren (17) and adult (14), preschool children (5). One hundred forty eight materials did not indicate the specific subjects; Thirty institutes had been providing the information for sexual education and the counseling. According to the above results. we suggest the followings: The systematic development of materials for sexual education appropriate to the specific subjects should be done: Materials in the CD-ROM and internet homepage should be developed more and more; The evaluation tool should be developed and adapted to the materials for the recommendation of the good materials; Database of the sexual materials should be provided for further development, evaluation and utilization.

• Journal of Microbiology and Biotechnology
• /
• 제16권12호
• /
• pp.1841-1848
• /
• 2006

Insights into gene expression have the potential for improvement of antibiotic yield and the development of robust production hosts for use in recombinant biomolecule production. $Cubicin^{TM}$ (daptomycin for injection) is a recently approved antibiotic active against many Gram(+) pathogens, including those resistant to methicillin, vancomycin, and fluoroquinolones. Daptomycin is produced as a secondary metabolite by Streptomyces roseosporus. A 128 kb region of DNA including the daptomycin biosynthetic gene cluster (dpt) has been cloned. and sequenced. Using a selected array of nucleic acid probes representing this region, we compared the expression levels of the dpt genes between S. roseosporus wild-type (WT) and derived S. roseosporus high-producer of daptomycin (HP). We observed that the majority of the biosynthetic genes were upregulated in HP compared with WT; a total of 12 genes, including those encoding daptomycin synthetase, showed consistently and significantly higher expression levels, at least 5-fold, in HP compared with WT. In contrast, some genes, flanking the dpt cluster, were expressed at higher levels in the WT strain. The expression of housekeeping genes such as S. roseosporus rpsL, rpsG, and 16S (positive controls) and presumptive intergenic regions in the dpt cluster (negative control) were identical in the two strains. In addition, we compared transcription during the early, mid-log, and early-stationary phases of growth in the HP strain. The same set of genes was upregulated and downregulated under all conditions examined; housekeeping genes showed no relative change in expression level over the periods of growth tested. Analyses of this type would be of value in studies of strain improvement and also for the identification of gene regulation processes that are important for secondary metabolite production.

• 한국농공학회논문집
• /
• 제49권5호
• /
• pp.67-77
• /
• 2007

The images of MODerate resolution Imaging Spectroradiometer (MODIS) that provide wider swath and shorter revisit frequency than Land Satellite (Landsat) and Satellite Pour I' Observation de la Terre (SPOT) has been used fer land cover classification with better spatial resolution than National Oceanic and Atmosphere Administration/Advanced Very High Resolution Radiometer (NOAA/AVHRR)'s images. Due to the advantages of MODIS, several researches have conducted, however the results for the land cover classification using MODIS images have less accuracy of classification in small areas because of low spatial resolution. In this study, uncertainty of paddy fields classification using MODIS images was conducted in the region of Gyeonggi-do and the relation between this uncertainty of estimating paddy fields and topographical factors was also explained. The accuracy of classified paddy fields was compared with the land cover map of Environmental Geographic Information System (EGIS) in 2001 classified using Landsat images. Uncertainty of paddy fields classification was analyzed about the elevation and slope from the 30m resolution Digital Elevation Model (DEM) provided in EGIS. As a result of paddy classification, user's accuracy was about 41.5% and producer's accuracy was 57.6%. About 59% extracted paddy fields represented over 50 uncertainty in one hundred scale and about 18% extracted paddy fields showed 100 uncertainty. It is considered that several land covers mixed in a MODIS pixel influenced on extracted results and most classified paddy fields were distributed through elevation I, II and slope A region.

• Journal of Microbiology and Biotechnology
• /
• 제11권2호
• /
• pp.222-228
• /
• 2001

Vibrio metschnikovii strain RH530 is a non-pathogenic, industrially-important alkaline protease producer which has been isolated from wastewater. In this paper, we report on the transformation of this strain by using the method of electroporation. A field strength of $7.5\;kVcm^{-1}$ and $25\;{\mu}F$, and using a 0.2-cm cuvette, appeared to be the optimal conditions for electroporation of the cells with the recombinant pSBCm plasmid carrying the vapK alkaline protease gene and the ColE1 replicon. Cells were subjected to osmotic shock in order to remove extracelluar DNase, and adding 200 mM of sucrose to electroporation buffer cells showed an increased transformation efficiency. Maximum efficiency of transformation was obtained at an early exponential growth phase. Using all of the conditions mentioned above, we routinely obtained a transformation efficiency of more than $10^4{({\mu}g\;plasmid\;DNA)}^{-1}$. The stability of the plasmid pSBCm in V. metschnikovii RH530 was 25% after 18h of growth (27 generations) in the medium without antibiotic selection. The insertion of the par locus to the pSBCm increased the stability of the plasmid up to 42% without selective pressure. The increase in plasmid stability was accompanied by the increase in the productivity of alkaline protease in the recombinant V. metschnikovii strain RH530. Determining optimal conditions for the transformation of the industrially-important, nonpathogenic Vibrio strain, and the improvement of plasmid stability by introducing the par locus into the high copy number plasmid vector, will allow the development of procedures involved in the genetic manipulation of this strain, particularly for its use in the production of industrial enzymes such as alkaline protease.

• Journal of Microbiology and Biotechnology
• /
• 제23권8호
• /
• pp.1116-1122
• /
• 2013

Cell line development is the most critical and also the most time-consuming step in the production of recombinant therapeutic proteins. In this regard, a variety of vector and cell engineering strategies have been developed for generating high-producing mammalian cells; however, the cell line engineering approach seems to show various results on different recombinant protein producer cells. In order to improve the secretory capacity of a recombinant tissue plasminogen activator (t-PA)-producing Chinese hamster ovary (CHO) cell line, we developed cell line engineering approaches based on the ceramide transfer protein (CERT) and X-box binding protein 1 (XBP1) genes. For this purpose, CERT S132A, a mutant form of CERT that is resistant to phosphorylation, and XBP1s were overexpressed in a recombinant t-PA-producing CHO cell line. Overexpression of CERT S132A increased the specific productivity of t-PA-producing CHO cells up to 35%. In contrast, the heterologous expression of XBP1s did not affect the t-PA expression rate. Our results suggest that CERT-S132A-based secretion engineering could be an effective strategy for enhancing recombinant t-PA production in CHO cells.

• Journal of Microbiology and Biotechnology
• /
• 제24권10호
• /
• pp.1427-1437
• /
• 2014

Enzymatic hydrolysis of lignocellulosic biomass into fermentable sugars is a key step in the conversion of agricultural by-products to biofuels and value-added chemicals. Utilization of a robust microorganism for on-site production of biomass-degrading enzymes has gained increasing interest as an economical approach for supplying enzymes to biorefinery processes. In this study, production of multi-polysaccharide-degrading enzymes from Aspergillus aculeatus BCC199 by solid-state fermentation was improved through the statistical design approach. Among the operational parameters, yeast extract and soybean meal as well as the nonionic surfactant Tween 20 and initial pH were found as key parameters for maximizing production of cellulolytic and hemicellulolytic enzymes. Under the optimized condition, the production of FPase, endoglucanase, ${\beta}$-glucosidase, xylanase, and ${\beta}$-xylosidase was achieved at 23, 663, 88, 1,633, and 90 units/g of dry substrate, respectively. The multi-enzyme extract was highly efficient in the saccharification of alkaline-pretreated rice straw, corn cob, and corn stover. In comparison with commercial cellulase preparations, the BCC199 enzyme mixture was able to produce remarkable yields of glucose and xylose, as it contained higher relative activities of ${\beta}$-glucosidase and core hemicellulases (xylanase and ${\beta}$-xylosidase). These results suggested that the crude enzyme extract from A. aculeatus BCC199 possesses balanced cellulolytic and xylanolytic activities required for the efficient saccharification of lignocellulosic biomass feedstocks, and supplementation of external ${\beta}$-glucosidase or xylanase was dispensable. The work thus demonstrates the high potential of A. aculeatus BCC199 as a promising producer of lignocellulose-degrading enzymes for the biomass conversion industry.

• 한국통신학회논문지
• /
• 제31권12C호
• /
• pp.1165-1172
• /
• 2006

소프트웨어의 발전으로 인해 디지털 컨텐츠에 대한 복사, 변경이 손쉽게 일반인들에 의해서도 가능하게 되어 컨텐츠의 제작자는 소유권 수장이나 내용의 변질에 대한 인증과 검증을 필요로 하게 되었다. 변질검증(Fragile) 워터마킹 기법은 워터마크가 삽입된 영상의 변질 및 훼손 여부를 판별할 수 있지만, 고의적이지 않은 표준화된 영상압축 등에서도 워터마크가 사라지는 문제점이 있다. 본 논문에서 JPEG 압축과 같은 영상처리에서는 강인하지만 변질에는 약한 Semi-Fragile 워터마킹 방법을 제안하였다. 제안한 방법은 블록간의 상관관계를 이용하여 워터마크를 생성하고, DCT(Discrete Cosine Transform)수행 후에 계수 값들의 양자화를 이용하여 HVS(Human Visual System)에 둔감한 부분의 계수 값에 삽입하는 방법을 제안하였다. 실험결과 공간영역 상에서 미세한 변화에도 변질여부의 검출 정도가 뛰어났으며, 변질된 부분을 블록별로 검출할 수 있어 공간상의 어떤 위치의 픽셀들이 변화하였는지를 시각적으로 확인할 수 있었다.

• 한국융합학회논문지
• /
• 제9권7호
• /
• pp.155-162
• /
• 2018

본 연구는 광고 이미지와 텍스트를 활용한 광고 제작 수업을 통해 광고 이미지와 텍스트의 관계를 탐구하고, 학생작품을 롤랑 바르트의 기호학으로 분석하였다. 제작자의 의도보다는 감상하는 수용자의 해석에 중점을 둔 바르트의 해석에 따라, 수용자로서 작품을 해석하였으며. 학생들이 작품에 어떤 의미를 담아 제작하였는지 사회 문화적 의미에서 분석하였다. 수업은 총 4차시로 D고등학교 1학년 2개 학급 총 64명을 대상으로 진행되었다. 광고 제작 수업 후 학생들의 광고작품을 기호학 입장에서 분석한 결과는 다음과 같다. 첫째, 바르트의 기호학 모형으로 분석한 결과, 학생들이 의도가 광고 이미지와 텍스트의 기호가 되어 의미작용을 하였다. 둘째, 광고 이미지와 텍스트가 서로 보완하는 역할을 하며 의미를 구성하는 상호관계성의 특성이 있다. 셋째, 학생들의 광고에 내재되어 있는 사회 문화적 의미를 끌어냄으로 그들의 가치관과 관심사를 발견할 수 있다.

• Journal of Evidence-Based Herbal Medicine
• /
• 제2권2호
• /
• pp.33-38
• /
• 2009

Korean Ginseng has been recognized as a representative special product in Korea and over the world for a long time, since its quality is known to be superior to Chinese, North American Ginseng. However, the export volume of Korean Ginseng products has been diminishing since 1990 because the imports of low price Chinese Ginseng and the effective marketing policy on North American Ginseng. Therefore, this study is to suggest the competitiveness reinforcement strategies through analyses of the present state and transition of the international competitiveness in Korean Ginseng. This study conducts the comparative analysis of international competitive power of ginseng between Korea and other exporting countries. There are many kinds of saponin that are competitive from a quality profile. However, price competitiveness was very low. According to the result of analysis, this study suggest the competitiveness reinforcement strategies of Korean Ginseng as follows ; First, it is necessary to establish the ginseng plan for high-quality environmentally-friendly production. Second, Korean Ginseng producer should develop various consumer-oriented products according to purchasing power and taste of target market consumers. Third, export strategies must be established by finding out every importing country’s characteristics with regard its import, circulation and consumption of the Korean Ginseng. The use of this study is to forecast useful information to concerned organization for the future policies to the ginseng products in the international market.