The aim of the present study was to investigate the effects of several fractions obtained from methylene chloride ($CH_2Cl_2$) extract of self-fermented pine needle (SFPNE) on the acetylcholine (ACh)-evoked CA release from the isolated perfused model of the rat adrenal medulla and to establish the mechanism of the most active fraction (Fr.)-induced inhibitory action on the CA release. We obtained 6 fractions from $CH_2Cl_2$ extract of self-fermented pine needle. For the ACh (5.32 mM)-evoked CA release, the following rank order of inhibitory potency was obtained: Fr.4-5 > Fr.8-11 ${\gg}$ Fr.3 > Fr.6 = Fr.7 > Fr.1-2. Fr. 4 - 5 (60 ${\mu}g/mL$) perfused into an adrenal vein for 90 min produced relatively time-dependent inhibition of the CA secretory responses to ACh (5.32 mM), DMPP (100 ${\mu}M$), McN-A-343 (100 ${\mu}M$) and high $K^+$ (56 mM). Fr. 4 - 5 itself did not affect basal CA secretion. Also, in the presence of Fr. 4 - 5 (60 ${\mu}g/mL$), the CA secretory responses to angiotensin II (AngII, 0.1 ${\mu}M$), veratridine (50 ${\mu}M$), Bay-K-8644 (10 ${\mu}M$), and cyclopiazonic acid (10 ${\mu}M$) were significantly reduced, respectively. In the simultaneous presence of Fr. 4 - 5 (60 ${\mu}g/mL$) and L-NAME (30 ${\mu}M$), the inhibitory responses of Fr. 4 - 5 on the CA secretion evoked by ACh, DMPP, high $K^+$, AngII, Bay-K-8644 and veratridine were considerably recovered to the extent of the corresponding control secretion compared with that of Fr. 4 - 5-treatment alone. The level of NO released from adrenal medulla after the treatment of Fr. 4 - 5 (60 ${\mu}g/mL$) was greatly elevated compared with the basal level. Taken together, these results demonstrate that Fr. 4 - 5 inhibits the CA secretion from the isolated perfused rat adrenal medulla evoked by stimulation of cholinergic receptors as well as by direct membrane-depolarization. It seems that this inhibitory effect of Fr. 4 - 5 is mediated by blocking the influx of $Ca^{2+}$ and $Na^+$ into the adrenomedullary chromaffin cells as well as by inhibition of $Ca^{2+}$ release from the cytoplasmic calcium store, which is evoked at least partly through the increased NO production due to the activation of NO synthase. Based on these results, it is also thought that Fr. 4 - 5 isolated from $CH_2Cl_2$ extract of pine needle may contain beneficial antihypertensive components to prevent or treat hypertension.
With the subjects of male consumers in their 20s to 40s living in the Ningbo area in Zhejiang Province, this study aims to investigate into the reality of their purchase attitude and size fitness of ready-made suits. The results are as follows; Looking into their demographic characteristics, 70.6% of the subjects were twenties, 60.6% were single, and educational career stood in the order of college, middle school, and high school graduation. They were largely absorbed in free trade, followed by teaching, commerce and service industry. 59.6% of them were Zhejiang Province belongs. One to two thousand yuan was the greatest portion of their monthly income. As for their purchase attitude of ready-made suits, they thought higher of material, quality, activity, and solidity than of design. They preferred to buy clothes at a department store. There was significant difference between purchase frequency and purchase price according to monthly income and jobs. Concerning brand recognition, the Chinese subjects favored "Youngor." Though Korea's brands were very lowly recognized, Korean products received really high recognition. Compared with China's brands, they found foreign brands excellent in design, followed by material/matter, wear, and sewing. As to their physical satisfaction and the size fitness of clothes, most subjects felt happy with their sizes. Trousers and jackets were among the unfitting suit items, while the girth of waist was the least satisfactory size. Therefore, in order to raise the market occupation rate of Korean suit goods in China, more aggressive marketing strategies are required to utilize the current Korean-style entertainment and maximize concerning brand images. In particular, outstanding products in consideration of prices should be made through the proper patternmaking to reflect the body types of the Chinese.
This study attempted to understand the perception and the degree of trust that consumers had in the national agricultural food accreditation system, to inquire into and examine the consumers' perception of traditional food quality certification performed for the protection of excellent traditional food, and to propose the right direction for the policies on the traditional food quality certification system and the plans to improve consumers' perception of the system. According to the results of this survey performed in married women, based on the 'the recognition of accreditation system' and 'the recognition of certification marks' of 'the seventh national agricultural food accreditation system' presently being used by the Ministry for Food, Agriculture, Forestry and Fisheries, the household food purchasers showed high recognition for eco-friendly agricultural product accreditation, processed food KS certification, and organically processed food certification in that order. Meanwhile, it was shown that they indicated low recognition for the traditional food-related accreditation systems such as 'traditional food quality certification' and 'food grand master'. It was found that reliability of the certification mark provided by the agricultural food accreditation system gained 3.54 points (on a 5-point scale), and 68.1% of the study subjects referred to the certification marks while purchasing agricultural foods. And most of them answered to the question of 'why they referred to the certification marks' saying that it was done 'to choose safe food'. The most frequent answer to question of 'the means to recognize the traditional food quality certification system' was 'broadcasting, advertising'. 57.3% of the subjects had a previous experience of buying a product certified by the traditional food quality certification system, and 93.2% of all the subjects had the intention to buy a product certified by the traditional food quality certification system later. And most of them answered that 'strengthening government policies' would be the most ideal way to inform the public of the traditional food quality certification system. According to the results of this study, while 'relying on the traditional food quality certification system in general' was relatively high among the consumers, 'intention to buy quality certified products by visiting a distantly located store' was low; thus, from this analysis, it can be seen that there is a need for diversification of places selling these quality certified products and to establish a distribution network for these products.
There seems to be some controversy about the effect of total ginseng saponin (TGS) on the secretion of catecholamines (CA) from the adrenal gland. Therefore, the present study aimed to determine whether TGS can affect the CA release in the perfused model of the adrenal medulla isolated from spontaneously hypertensive rats (SHRs). TGS (15-150 ${\mu}g/mL$), perfused into an adrenal vein for 90 min, inhibited the CA secretory responses evoked by acetylcholine (ACh, 5.32 mM) and high $K^+$ (56 mM, a direct membrane depolarizer) in a dose- and time-dependent fashion. TGS (50 ${\mu}g/mL$) also time-dependently inhibited the CA secretion evoked by 1.1-dimethyl-4 -phenyl piperazinium iodide (DMPP; 100 ${\mu}M$, a selective neuronal nicotinic receptor agonist) and McN-A-343 (100 ${\mu}M$, a selective muscarinic M1 receptor agonist). TGS itself did not affect basal CA secretion (data not shown). Also, in the presence of TGS (50 ${\mu}g/mL$), the secretory responses of CA evoked by veratridine (a selective $Na^+$ channel activator (50 ${\mu}M$), Bay-K-8644 (an L-type dihydropyridine $Ca^{2+}$ channel activator, 10 ${\mu}M$), and cyclopiazonic acid (a cytoplasmic $Ca^{2+}$-ATPase inhibitor, 10 ${\mu}M$) were significantly reduced, respectively. Interestingly, in the simultaneous presence of TGS (50 ${\mu}g/mL$) and N${\omega}$-nitro-L-arginine methyl ester hydrochloride [an inhibitor of nitric oxide (NO) synthase, 30 ${\mu}M$], the inhibitory responses of TGS on the CA secretion evoked by ACh, high $K^+$, DMPP, McN-A-343, Bay-K-8644, cyclopiazonic acid, and veratridine were considerably recovered to the extent of the corresponding control secretion compared with the inhibitory effect of TGS-treatment alone. Practically, the level of NO released from adrenal medulla after the treatment of TGS (150 ${\mu}g/mL$) was greatly elevated compared to the corresponding basal released level. Taken together, these results demonstrate that TGS inhibits the CA secretory responses evoked by stimulation of cholinergic (both muscarinic and nicotinic) receptors as well as by direct membrane-depolarization from the isolated perfused adrenal medulla of the SHRs. It seems that this inhibitory effect of TGS is mediated by inhibiting both the influx of $Ca^{2+}$ and Na+ into the adrenomedullary chromaffin cells and also by suppressing the release of $Ca^{2+}$ from the cytoplasmic calcium store, at least partly through the increased NO production due to the activation of nitric oxide synthase, which is relevant to neuronal nicotinic receptor blockade, without the enhancement effect on the CA release. Based on these effects, it is also thought that there are some species differences in the adrenomedullary CA secretion between the rabbit and SHR.
Journal of Advanced Marine Engineering and Technology
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제40권1호
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pp.1-9
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2016
굴은 향기가 좋고 영양성분이 풍부하며 질병 예방의 기능성이 높은 우수한 수산식품으로 동서양인 모두가 선호한다. 대량으로 생산된 굴의 보관 및 유통의 편의성을 증가시키는 가장 좋은 방법은 건조하는 것인데, 기존의 열풍건조나 동결건조에 의할 경우에는 굴의 육질이 매우 약하여 향과 영양 성분의 파괴가 많아 완전건조가 불가능하다. 본 논문에서는 $15^{\circ}C$ 저온진공건조기술을 이용하여 한국산 양식 생굴과 자연산 생굴을 완전 건조하는 과정에서 진공상태하의 진공열전달특성에 관한 실험적 결과를 보고하였다.
Thermal storage plays an important role in building energy saving, which is greatly assisted by the incorporation of latent heat storage in building materials. A phase change material is a substance with a high heat of fusion which, melting and solidifying at a certain temperature, can be storing and releasing large amount of energy. Heat is stored or released when the material changes from solid to liquid. Integration of building materials incorporating PCMs into the building envelope can result in increased efficiency of the built environment. The aim of this research is to identify thermal performance of PCMs impregnated building materials which is applied to interior of building such as gypsum and red clay. In order to analyze thermal performance of phase change materials, test-cell experiments and simulation analysis were carried out. The results show that micro-encapsulated PCM has an effect to maintain a constant indoor temperature using latent heat through the test-cell experiments. PCM wallboard makes it possible to reduce the fluctuation of room temperature and heating and cooling load by using EnergyPlus simulation program. Phase change material can store solar energy directly in buildings. Increasing the heat capacity of a building is capable of improving human comfort by decreasing the frequency of indoor air temperature swings so that the interior air temperature is closer to the desired temperature for a long period of time.
This study was to investigate medication management and the level of awareness about separation of prescribing and dispending at small-to-medium sized workplaces which have less than 300 employees in Korea. The data were collected by questionnaires from May to June in 2000. The number of subjects were 127 workplaces and 130 employees. The SAS PC Program was used for the descriptive statistics. The results are as follows; 1. The over the counter(OTC) drug was provided sufficiently(91.1 %). but medication management was not performed systematically. 2. On drug-providing rate and drug-using rate, the latter was high in the workplace and Health care management's nurses consume all of the over the counter drug provided. 3. When the separation of prescribing and dispending starts, employees shall be medically examined, treated and prescribed by physicians and drugs shall be dispended by pharmacists. It is necessary to make it possible to visit health care institutions whenever they want to. (eg. lunch time or after work) They should change their drug-dependent behaviour. We should focus on strengthening Health Promotion Program to prevent disease by making habitual of health promotion behaviour.
Purpose - The recent franchise industry is rapidly developing. Some franchisees have a low barriers to entry and competition among companies is intensifying. In this dynamic competitive environment, companies need to focus on customer preferences, quality, and technical interfaces to gain competitive advantage. As a result, companies are required to measure the performance of service values in order to provide differentiated services from competitors. In the franchise industry, customer experience marketing of service values will enable companies to create new businesses. Franchise firms should explore a variety of services to increase service value and reduce failures. Research design, data, methodology - The questionnaire of this study was based on the previous research. Surveys were conducted on panels of online surveys. Surveys were conducted on the panel who had visited the restaurant franchise within the past month. The survey was conducted for about 7 days from February 13, 2019 to February 19, 2019. Total 300 samples, 293 were used in the analysis except for seven unfair questionnaires. Results - The findings of this study are as follows: Emotional, monetary, and reputation values have positive effects on cognitive and affective attitudes. Quality value and behavioral value did not effect cognitive attitude and affective attitude significantly. In addition, affective attitude has positive effect on loyalty, but cognitive attitude did not significant effect on loyalty. Conclusions - First, food-service franchise company should develop a service that enables customers to use the store conveniently. We need to develop a comfortable environment for our customers and provide intangible services. Second, food-service franchise company should provide a reasonable price service. Food-service franchise company needs to sell a high quality menu at a reasonable price to generate profits. Third, food-service franchise companies need to strategically respond to their reputation. In other words, food-service franchise company needs to constantly monitor the reputation of its customers and respond appropriately to market conditions. Fourth, food-service franchise company needs to develop a service method capable of emotional interaction with customers. Food-service franchise firms need to develop ongoing service methods and educate their staff.
Saifullah Jamali;Muhammad Aslam Khoso;Irfan Ali Sanjrani;Hussain Saleem;Tariq Ali Siyal;Muhammad Ashraf;Mansoor Ahmed Memon;Ghulam Murtaza;Zahid Hussain Arain;Zaheer Ahmed Ujjan;Muhammad Niaz Laghari;Samina Saleem;Nek M. Shaikh;Waseem A. Bhutto;Abdul Majid Soomro;Altaf H. Nizamani
International Journal of Computer Science & Network Security
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제23권3호
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pp.193-202
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2023
Numerous articles under the study and the examination of heavy metals in human teeth have been published in recent years. The heavy metal poisoning is a widespread issue emerged in toxicology area these days. It has been discovered that long-term exposure to heavy metals typically present in traces, in our everyday meals, drinking water, and in the environment as pollution causes heavy metal poisoning in human beings. Industrial effluents, Coal and Oil, as well as a variety of consumer items, such as cosmetics, can all cause this type of exposure. Teeth, which are often thought of as exoskeleton parts, store heavy metals with a high affinity and represent long-term exposure information. In this study, we have chosen and examined the sections of dentine instead, then examined the entire tooth. We have combined the work done on the examination of heavy metals in human teeth using several instrumental approaches e.g. "Optical Spectroscopic Techniques" to detect elemental profile of human teeth in the current study.
The fast emerging of network technology and the high demand of computing resources have prompted many organizations to outsource their storage and computing needs. Cloud based storage services such as Microsoft's Azure and Amazon's S3 allow customers to store and retrieve any amount of data, at anytime from anywhere via internet. The scalable and dynamic of the cloud storage services help their customer to reduce IT administration and maintenance costs. No doubt, cloud based storage services brought a lot of benefits to its customer by significantly reducing cost through optimization increased operating and economic efficiencies. However without appropriate security and privacy solution in place, it could become major issues to the organization. As data get produced, transferred and stored at off premise and multi tenant cloud based storage, it becomes vulnerable to unauthorized disclosure and unauthorized modification. An attacker able to change or modify data while data inflight or when data is stored on disk, so it is very important to secure data during its entire life-cycle. The traditional cryptography primitives for the purpose of data security protection cannot be directly adopted due to user's lose control of data under off premises cloud server. Secondly cloud based storage is not just a third party data warehouse, the data stored in cloud are frequently update by the users and lastly cloud computing is running in a simultaneous, cooperated and distributed manner. In our proposed mechanism we protect the integrity, authentication and confidentiality of cloud based data with the encrypt- then-upload concept. We modified and applied proxy re-encryption protocol in our proposed scheme. The whole process does not reveal the clear data to any third party including the cloud provider at any stage, this helps to make sure only the authorized user who own corresponding token able to access the data as well as preventing data from being shared without any permission from data owner. Besides, preventing the cloud storage providers from unauthorized access and making illegal authorization to access the data, our scheme also protect the data integrity by using hash function.
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