• Food Science and Biotechnology
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• v.18 no.3
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• pp.689-693
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• 2009

Germination is well-known to enhance the digestibility, functionality, and palatability of plant seeds. To examine the functionality of germinated-safflower seed (GSS), proliferative and differentiative effects of GSS extract on the mouse calvarial bone cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolinbromide (MTT) assay and alkaline phosphatase activity, respectively. Water extract of GSS increased dose-dependently proliferative and differentiative effects on calvarial bone cell, and its effects were stronger than those of ungerminated-safflower seeds (UGSS) extract. One major component was isolated from GSS extract by a series of purification procedure of solvent fractionation, Diaion HP-20, and Sephadex LH-20 column chromatographies. Its chemical structure was identified as trachelogenin (TC) by nuclear magnetic resonance (NMR) and mass spectrometry (MS) spectral analysis. Trachelogenin showed significant proliferative (125.7%) and differentiative (132.1%) effects on calvarial bone cells at $10^{-8}M$, and its effects were significantly higher than those of $17{\beta}-estradiol\;(E_2)$. TC was found to be a major active compound responsible for high proliferative and differentative effects of the water extract of GSS. Therefore, these results suggest that TC in GSS may be useful as potential therapeutic agent for the prevention and treatment of bone loss.

• Journal of Korean Society of Water and Wastewater
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• v.34 no.1
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• pp.1-8
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• 2020

In this study, the physicochemical characteristics and fluoride adsorption capacity of the bone char pyrolyzed at different temperatures; 200℃, 300℃, 350℃, 400℃, 500℃, 600℃, and 700℃ were investigated. Analytical studies of the synthesized bone char including; SEM-EDS, XRD, BET and FT-IR, showed the presence of hydroxyapatite(HAP), which is the main substance that adsorbs fluoride from aqueous solutions containing high fluoride concentrations. Bone char pyrolyzed from 350~700℃ specifically revealed that, the lower the temperature, the higher the fluoride adsorption capacity and vice versa. The loss of the fluoride adsorption function of HAP (OH^- band in the FTIR analysis) was interpreted as the main reason behind this inverse correlation between temperature and fluoride adsorption. Bone char produced at 350℃ hence exhibited a fluoride adsorption capacity of 10.56 mgF/g, resulting in significantly higher adsorption compared to previous studies.

• International Journal of Industrial Entomology and Biomaterials
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• v.32 no.2
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• pp.49-53
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• 2016

Bone morphogenetic proteins (BMPs) are essential growth factors for bone formation, skeletal development and bone regeneration. The BMP-2/7 heterodimer is known to have remarkable effects on osteogenic induction that are even stronger than the BMP-2 or BMP-7 homodimers. We designed a recombinant human BMP-2/7 (rhBMP-2/7) heterodimer protein with four glycine residues between BMP-2 and BMP-7 protein to facilitate free bond rotation of domains. The Baculovirus Expression Vector System (BEVS) is routinely used to produce recombinant proteins in the milligram scale. In this study, the BEVS was used to express the rhBMP-2/7 protein whrer the recombinant baculovirus was recovered in the host Sf9 cells. To confirm the biological activity of rhBMP-2/7 protein secreted from the BEVS as an osteogenic differentiation and induction factor, we measured the BMP-induced ALP activity. rhBMP-2/7 could be used as an alternative to BMPs to overcome limitations like short half-life and requirement for high concentrations. Furthermore, rhBMP-2/7 may be an efficient tool for various application studies such as bone regeneration and skeletal development.

• Asian-Australasian Journal of Animal Sciences
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• v.7 no.4
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• pp.555-561
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• 1994

This paper presents results from a study of the body composition and sensory characteristics of female weaner goat meat. A completely randomized $3{\times}3{\times}2$ factorial design was used. Factors were genotype (Thai native; TN, 75% TN $\times$ 25% Anglo-Nubian; AN and 50% TN $\times$ 50% AN), feeding {grazing only, low (1.0% BW/d) and high (1.5% BW/d) concentrate supplementation and parasite control (undrenched and drenched)}. It was shown that there was no effect of genotype on body components and dressing percentage. However, TN and 75% TN $\times$ 25% AN kids had significantly (p<0.05) higher muscle to bone ratios (4.20% and 4.20%, respectively) compared with 50% TN $\times$ 50% AN kids (3.88%). Kids on grazing only had significantly (p<0.01) higher muscle percentage (64.12%) than did kids in low (61.30%) and high (60.62%) supplementary feeding program, but there was no significant (p>0.05) difference between low and high supplementary feeding groups. Kids offered supplementary feeding had significantly (p<0.01) higher percentages of total fat, intermuscular fat, pelvic fat and kidney fat than those of grazing only. Kids offered supplementary feeding had significantly (p<0.05) higher muscle to bone ratios and significantly (p<0.01) higher muscle plus fat to bone ratios compared with those of grazing only. This may be due to significantly lower (p<0.01) bone contents (14.95, 14.17 and 16.8% for kids offered low and high supplementary feeding and grazing only, respectively. There was no significant difference in sensory characteristics of goat meat between genotypes or feeding groups.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.4
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• pp.1707-1710
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• 2014

Background: The objective of this study was to evaluate baseline use and positive rates of staging images (bone scan, CT) in newly diagnosed patients with prostate cancer (PCa) and to improve staging image overuse. Materials and Methods: This retrospective study covered a consecutive series of patients with PCa who underwent stage imaging at our institution between 2006 and 2011. Various clinical and pathological variables (age, PSA, biopsy Gleason score, clinical T stage, positive biopsy core rate) were evaluated by multivariate logistic regression analysis for their ability to predict a positive staging image. All patients were stratified according to the NCCN risk stratification and positive rates were compared in each risk group. Results: 410 patients (100%) underwent a bone scan and 315 patients (76.8%) underwent a CT scan. Some 51 patients (12.4%) had a positive bone scan, clinical T3 and T4 being significant independent predictors. Positive bone scan rates for low-, intermediate-, high-, and very high-risk groups were 0%, 0%, 8.25%, and 56.6%. Some 59 (18.7%) patients had a positive CT scan, with elevated PSA and clinical T3, T4 as significant independent predictors. Low-, intermediate-, high- and very high-risk group rates were 0%, 0%, 13.8% and 80.0%. Conclusions: The incidences of positive staging image in low- and intermediate- risk group were reasonably low. Following feedback on these results, staging in low- and intermediate- risk groups could be omitted.

• The Journal of Korean Academy of Prosthodontics
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• v.45 no.4
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• pp.506-521
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• 2007

Statement of problem: The cumulative success rate of wide implant is still controversial. Some previous reports have shown high success rate, and some other reports shown high failure rate. Purpose: The aim of this study was to analyze, and compare the biomechanics in wide implant system embeded in different width of crestal bone under different occlusal forces by finite element approach. Material and methods: Three-dimensional finite element models were created based on tracing of CT image of second premolar section of mandible with one implant embedded. One standard model (6mm-crestal bone width, 4.0mm implant diameter central position) was created. Varied crestal dimension(4, 6, 8 mm), different diameter of implants(3.3, 4.0, 5.5, 6.0mm), and buccal position implant models were generated. A 100-N vertical(L1) and 30 degree oblique load from lingual(L2) and buccal(L3) direction were applied to the occlusal surface of the crown. The analysis was performed for each load by means of the ANSYS V.9.0 program. Conclusion: 1. In all cases, maximum equivalent stress that applied $30^{\circ}$ oblique load around the alveolar bone crest was larger than that of the vertical load. Especially the equivalent stress that loaded obliquely in buccal side was larger. 2. In study of implant fixture diameter, stress around alveolar bone was decreased with the increase of implant diameter. In the vertical load, as the diameter of implant increased the equivalent stress decreased, but equivalent stress increased in case of the wide implant that have a little cortical bone in the buccal side. In the lateral oblique loading condition, the diameter of implant increased the equivalent stress decreased, but in the buccal oblique load, there was not significant difference between the 5.5mm and 6.0mm as the wide diameter implant. 3. In study of alveolar bone width, equivalent stress was decreased with the increase of alveolar bone width. In the vertical and oblique loading condition, the width of alveolar bone increased 6.0mm the equivalent stress decreased. But in the oblique loading condition, there was not a difference equivalent stress at more than 6.0mm of alveolar bone width. 4. In study of insertion position of implant fixture, even though the insertion position of implant fixture move there was not a difference equivalent stress, but in the case of little cortical bone in the buccal side, value of the equivalent stress was most unfavorable. 5. In all cases, it showed high stress around the top of fixture that contact cortical bone, but there was not a portion on the bottom of fixture that concentrate highly stress and play the role of stress dispersion. These results demonstrated that obtaining the more contact from the bucco-lingual cortical bone by installing wide diameter implant plays an important role in biomechanics.

• Journal of Trauma and Injury
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• v.32 no.4
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• pp.202-209
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• 2019

Purpose: Cranioplasty (CP) is often required for survival after decompressive craniectomy. Several materials, including autologous bone and various artificial materials, have been introduced for CP, but it remains unclear which material is best for CP. This study aimed to explore differences in complications between patients who underwent CP using an autologous bone flap versus a three-dimensional (3D) titanium mesh and to identify significant risk factors for post-CP complications. Methods: In total, 44 patients were enrolled in this study and divided into two groups (autologous bone vs. 3D titanium mesh). In both groups, various post-CP complications were evaluated. Through a comparative analysis, we aimed to identify differences in complications between the two groups and, using binary logistic analysis, to determine significant factors associated with complications after CP. Results: In the autologous bone flap group, there were three cases of surgical infection (3/24, 12.5%) and 11 cases of bone flap resorption (BFR) (11/24, 45.83%). In the 3D titanium mesh group, there was only one case of surgical infection (1/20, 5%) and 11 cases of various complications, including mainly cosmetic issues (11/20, 55%). A subgroup risk factor analysis of CP with an autologous bone flap showed no risk factors that predicted BFR with statistical significance, although a marginal association was found between larger bone flaps and BFR (odds ratio [OR]=1.037, p=0.090). In patients treated with a 3D titanium mesh, multivariate analysis revealed that only the existence of a ventriculo-peritoneal shunt system was strongly associated with overall post-CP complications (OR=18.66, p=0.021). Conclusions: Depending on which material was used, different complications could occur, and the rate of complications was relatively high in both groups. Hence, the material selected for CP should be selected based on individual patients' conditions.

• Korean Journal of Community Nutrition
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• v.1 no.1
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• pp.71-78
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• 1996

This study was conducted to compare the dietary factors which influence on the bone status of 28 women in urban and 30 women in rural area. Urinary excretion of hydroxyproline(Hpr) and Calcium(Ca) were measured as biological markers of bone resorption. Mean daily intake levels of total protein, animal protein, total calcium, calcium, calcium from milk and milk products, animal calcium, Ca / P ratio by 24 hr recall method were significantly higher in urban women. However, mean daily sodium(Na) intake levels were not significantly different between two groups. Ca Index score and Na Index score by food frequency methods were also significantly higher in urban than in rural subjects. While urinary Ca excretion elves of two groups were similar, Na excretion levels were significantly higher in rural women. Mean urniary levels of Ca / creatinine(cr) and Hpr / cr as bone status index were within normal range and not significantly different between two groups. However, prevalence of poor bone status as assessed by hydroxyproline was higher in rural women. Na Index, urinary Ca excretion and Ca / cr ratio were significantly correlated with bone status(Hpr / cr) in urban women, while only age was related to bone status in rural women. These demonstrated that high Na intake results in increased urinary excretion of Na and Ca and could cause bone resorption. Multiple regression analysis indicated that Na Index score and age have greater effect than other variables in urban women and only age has greater effect in rural women.

• Journal of Periodontal and Implant Science
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• v.48 no.2
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• pp.84-91
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• 2018

Purpose: The purpose of this study was to visualize and identify peri-implant bone defects in optical coherence tomography (OCT) images and to obtain quantitative measurements of the defect depth. Methods: Dehiscence defects were intentionally formed in porcine mandibles and implants were simultaneously placed without flap elevation. Only the threads of the fixture could be seen at the bone defect site in the OCT images, so the depth of the peri-implant bone defect could be measured through the length of the visible threads. To analyze the reliability of the OCT measurements, the flaps were elevated and the depth of the dehiscence defects was measured with a digital caliper. Results: The average defect depth measured by a digital caliper was $4.88{\pm}1.28mm$, and the corresponding OCT measurement was $5.11{\pm}1.33mm$. Very thin bone areas that were sufficiently transparent in the coronal portion were penetrated by the optical beam in OCT imaging and regarded as bone loss. The intraclass correlation coefficient between the 2 methods was high, with a 95% confidence interval (CI) close to 1. In the Bland-Altman analysis, most measured values were within the threshold of the 95% CI, suggesting close agreement of the OCT measurements with the caliper measurements. Conclusions: OCT images can be used to visualize the peri-implant bone level and to identify bone defects. The potential of quantitative non-invasive measurements of the amount of bone loss was also confirmed.

• Archives of Craniofacial Surgery
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• v.9 no.2
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• pp.62-66
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• 2008

Purpose: Although nasal fractures are often discussed as minor injuries but the incidence of post-traumatic nasal deformity remains high. For decrease the incidence of post-traumatic nasal deformity which require the guideline to optimize the management of acute nasal bone fracture. The aim of this study is analysis of post-traumatic nasal deformity according to Stranc classification. Method: We reviewed 310 patients with nasal bone fracture treated at our hospital from January of 2005 to December of 2006. Result: Post-traumatic complication were divided septal deviation, nasal bone deformity, temporary hyposmia and synechia. Nasal bone deformity include nasal bone deviation, hump, flat nose and minimal nasal bone irregularity. The incidence of total complication rate was 36.4%. The most common complication was nasal deformity(22.9%) followed by septal deviation(19.7%). The most common complication was septal deviation(20%) in frontal impact. The most common complication was nasal deformity(19.5%) in lateral impact. In frontal impact, the incidence of complication rate was plane II(68.8%) followed by plane I(29.9%) and plane III(16.7%). In lateral impact, the incidence of complication rate was plane II (78.8%) followed by plane III(61.5%) and plane I(42.7%). Conclusion: This result can be used to improve longterm results and to reduce the incidence of post-traumatic nasal deformity by predict complication of nasal bone fracture according to Stranc classification.