• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2021년도 학술발표회
• /
• pp.385-385
• /
• 2021

하천에서 실제로 유속 2.0m/s 이상 발생할 시 유량측정은 매우 급변하는 유속과 수위변화에 따른 측정값의 불확실성, 운영적인 측면에서의 시·공간적 한계 등으로 고유량에 대해 정확한 유량을 산정하기 어려운 실정이다. 그리고 국가하천은 최소 80년 빈도 이상, 지방하천은 최소 50년 빈도 이상의 확률강우량 채택을 통해 고유량에 해당하는 계획홍수량을 산정하고 있으나, 실제로 높은 호우의 빈도는 쉽게 발생하지 않아 유량측정성과가 부재하거나 매우 극소수에 불과한 상황이다. 따라서 유량측정성과는 대상하천의 계획홍수량(계획홍수위) 이하의 수준, 즉 중규모 수위 이하의 구간에서 대부분의 성과를 가지고 있으므로 고유량 산정은 고수위 외삽추정식에 의존할 수밖에 없다. 고수위 외삽추정은 대체로 기 유량측정성과(h, q)와 통수단면적(AD^1/2) 자료를 이용하는 Stevens 방법을 주로 이용하며, 이 방법은 하폭에 비해 수심이 비교적 작은, 얕은 하천과 기 유량측정성과가 추정하려는 고수위 구간에 근접한 경우에 적용성이 매우 용이하다고 할 수 있다. 설마천 유역 전적비교 수위관측소의 경우는 수위 4.110m까지 최대로 통수할 수 있으며, 하폭은 24.230m, 관측 최고수위는 3.194m, 유량측정성과 최대수위는 1.613m(40.303m³/s)이다. 설마천 유역에 대해 Stevens 방법을 적용하는 경우 위 조건을 만족하지 않으므로 다른 방법으로의 접근이 필요하다. AMC-III 조건의 선행강수량과 지속기간 1시간을 갖는 최대강우강도별 관측도달시간 자료를 통해 관계식을 유도하였으며, 강우 빈도해석의 결과인 지속기간 1시간의 빈도별 강우강도에 해당하는 도달시간을 유속으로 환산하는 과정을 거쳤다. 그 결과 유속은 1.808m/s(2년 빈도_43.3mm)~4.254m/s(500년 빈도_101.9mm)이며, 기 유량측정성과의 결과인 수위, 통수단면적, 유속, 유량, 최대강우강도(86.1mm_80년 빈도)가 발생했을 때의 해당 유속(도달시간 환산값), 수위, 통수단면적을 통해 최종적으로 빈도(년)별 유속, 수위, 유량을 결정하였다. 한국하천일람(2018)에서 제시된 설마천 전체 유역의 80년 빈도 계획홍수량(315m³/s, A=17.59km²) 값은 전적비교 수위관측소(A=8.48km²)와 직접적인 비교는 어렵지만, 유역면적비(0.482)를 적용한 추정된 계획홍수량은 약 152m³/s 볼 수 있다. 상기의 빈도별 유속, 수위, 통수단면적 결과인 80년 빈도(86.1mm)-유속(3.594m/s)-수위(3.194m)-통수단면적(53.197m²)에 해당하는 계산된 유량은 191.212m³/s로 분석되었다. 그리고 최대통수가 가능한 수위 4.110m의 계산된 유량은 313.674m³/s(약 424년 빈도 추정, 유속 4.203m/s, 통수단면적 74.761m²)로 결국에는 빈도(년)에 해당하는 수위-유량관계식(고수위 외삽추정식)을 통해 고유량을 산정할 수 있었다.

• Animal Bioscience
• /
• 제35권10호
• /
• pp.1489-1498
• /
• 2022

Objective: The objective of this study was to identify polymorphism in olfactomedin like 3 (OLFML3) gene, and association analysis with meat quality, carcass characteristics, retail meat cut, and fatty acid composition in sheep, and expression quantification of OLFML3 gene in phenotypically divergent sheep. Methods: A total of 328 rams at the age of 10 to 12 months with an average body weight of 26.13 kg were used. A novel polymorphism was identified using high-throughput sequencing in sheep and genotyping of OLFML3 polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Among 328 rams, 100 rams representing various sheep genotypes were used for association study and proc general linear model was used to analyse association between genotypes and phenotypic traits. Quantitative real-time polymerase chain reaction (qRT-PCR) was used for the expression analysis of OLFML3 mRNA in phenotypically divergent sheep population. Results: The findings revealed a novel polymorphism in the OLFML3 gene (g.90317673 C>T). The OLFML3 gene revealed three genotypes: CC, CT, and TT. The single nucleotide polymorphism (SNP) was found to be significantly (p<0.05) associated with meat quality traits such as tenderness and cooking loss; carcass characteristics such as carcass length; retail meat cut such as pelvic fat in leg, intramuscular fat in loin and tenderloin, muscle in flank and shank; fatty acids composition such as tridecanoic acid (C13:0), palmitoleic acid (C16:1), heptadecanoic acid (C17:0), ginkgolic acid (C17:1), linolenic acid (C18:3n3), arachidic acid (C20:0), eicosenoic acid (C20:1), arachidonic acid (C20:4n6), heneicosylic acid (C21:0), and nervonic acid (C24:1). The TT genotype was associated with higher level of meat quality, carcass characteristics, retail meat cut, and some fatty acids composition. However, the mRNA expression analysis was not different among genotypes. Conclusion: The OLFML3 gene could be a potential putative candidate for selecting higher quality sheep meat, carcass characteristics, retail meat cuts, and fatty acid composition in sheep.

• Journal of Ginseng Research
• /
• 제48권3호
• /
• pp.298-309
• /
• 2024

Background: 20(S)-ginsenoside Rh2(GRh2), an effective natural histone deacetylase inhibitor, can inhibit acute myeloid leukemia (AML) cell proliferation. Lactate regulated histone lactylation, which has different temporal dynamics from acetylation. However, whether the high level of lactylation modification that we first detected in acute promyelocytic leukemia (APL) is associated with all-trans retinoic acid (ATRA) resistance has not been reported. Furthermore, Whether GRh2 can regulate lactylation modification in ATRA-resistant APL remains unknown. Methods: Lactylation and METTL3 expression levels in ATRA-sensitive and ATRA-resistant APL cells were detected by Western blot analysis, qRT-PCR and CO-IP. Flow cytometry (FCM) and APL xenograft mouse models were used to determine the effect of METTL3 and GRh2 on ATRA-resistance. Results: Histone lactylation and METTL3 expression levels were considerably upregulated in ATRA-resistant APL cells. METTL3 was regulated by histone lactylation and direct lactylation modification. Overexpression of METTL3 promoted ATRA-resistance. GRh2 ameliorated ATRA-resistance by downregulated lactylation level and directly inhibiting METTL3. Conclusions: This study suggests that lactylation-modified METTL3 could provide a promising strategy for ameliorating ATRA-resistance in APL, and GRh2 could act as a potential lactylation-modified METTL3 inhibitor to ameliorate ATRA-resistance in APL.

• Biomolecules & Therapeutics
• /
• 제32권1호
• /
• pp.154-161
• /
• 2024

Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disorder that causes progressive paralysis. L-Citrulline is a nonessential neutral amino acid produced by L-arginine via nitric oxide synthase (NOS). According to previous studies, the pathogenesis of ALS entails glutamate toxicity, oxidative stress, protein misfolding, and neurofilament disruption. In addition, L-citrulline prevents neuronal cell death in brain ischemia; therefore, we investigated the change in the transport of L-citrulline under various pathological conditions in a cell line model of ALS. We examined the uptake of [¹⁴C]L-citrulline in wild-type (hSOD1wt/WT) and mutant NSC-34/ SOD1^G93A (MT) cell lines. The cell viability was determined via MTT assay. A transport study was performed to determine the uptake of [¹⁴C]L-citrulline. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was performed to determine the expression levels of rat large neutral amino acid transported 1 (rLAT1) in ALS cell lines. Nitric oxide (NO) assay was performed using Griess reagent. L-Citrulline had a restorative effect on glutamate induced cell death, and increased [¹⁴C]L-citrulline uptake and mRNA levels of the large neutral amino acid transporter (LAT1) in the glutamate-treated ALS disease model (MT). NO levels increased significantly when MT cells were pretreated with glutamate for 24 h and restored by co-treatment with L-citrulline. Co-treatment of MT cells with L-arginine, an NO donor, increased NO levels. NSC-34 cells exposed to high glucose conditions showed a significant increase in [¹⁴C]L-citrulline uptake and LAT1 mRNA expression levels, which were restored to normal levels upon co-treatment with unlabeled L-citrulline. In contrast, exposure of the MT cell line to tumor necrosis factor alpha, lipopolysaccharides, and hypertonic condition decreased the uptake significantly which was restored to the normal level by co-treating with unlabeled L-citrulline. L-Citrulline can restore NO levels and cellular uptake in ALS-affected cells with glutamate cytotoxicity, pro-inflammatory cytokines, or other pathological states, suggesting that L-citrulline supplementation in ALS may play a key role in providing neuroprotection.

• 한국식품저장유통학회지
• /
• 제30권2호
• /
• pp.235-246
• /
• 2023

Yellowball (Citrus hybrid cv. Yellowball ) is a new citrus hybrid between Haruka (C. tamurana × natsudaidai ) and Kiyomi (C. unshiu × sinensis) and is known to possess strong antioxidant activity. However, detailed information on the antioxidant components of its peel has not yet been reported. This study evaluated the antioxidant activity of the peel and identified the antioxidant components by fractionating a methanolic extract of Yellowball peels using liquid-liquid extraction with n-hexane, ethyl ether (ether), ethyl acetate (EA), butanol, and water. The phenolic contents and antioxidant activities of the n-hexane, ether, and EA fractions were higher than those of the other fractions, and these fractions were further separated by semi-preparative high-performance liquid chromatography (HPLC). Four antioxidant peaks, EA1, EA2, EA3, and He1, were isolated and analyzed using ultra-performance liquid chromatography-quadrupole-time- of-flight mass spectrometry (UPLC-Q-TOF MS). Sinapoyl glucoside and hesperidin were identified in EA2 and EA3, respectively, and a polymethoxylated flavone (PMF) complex (5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone, natsudaidain, tetrameth- oxyflavone, and tangeretin) was identified in He1. A compound in EA1 with m/z 223.0246 [M-H] could not be identified and was named unknown2. The antioxidant activity of unknown2 (IC₅₀=69.17 ㎍/mL) was similar to that of Trolox, which was noted as a major antioxidant in Yellowball peel. Further studies on the antioxidant capacity of Yellowball peel are required; however, these results provide a foundation for using Yellowball peel as an antioxidant.

• Korean Journal of Radiology
• /
• 제24권8호
• /
• pp.772-783
• /
• 2023

Objective: Imaging-based survival stratification of patients with gliomas is important for their management, and the 2021 WHO classification system must be clinically tested. The aim of this study was to compare integrative imaging- and pathology-based methods for survival stratification of patients with diffuse glioma. Materials and Methods: This study included diffuse glioma cases from The Cancer Genome Atlas (training set: 141 patients) and Asan Medical Center (validation set: 131 patients). Two neuroradiologists analyzed presurgical CT and MRI to assign gliomas to five imaging-based risk subgroups (1 to 5) according to well-known imaging phenotypes (e.g., T2/FLAIR mismatch) and recategorized them into three imaging-based risk groups, according to the 2021 WHO classification: group 1 (corresponding to risk subgroup 1, indicating oligodendroglioma, isocitrate dehydrogenase [IDH]-mutant, and 1p19q-codeleted), group 2 (risk subgroups 2 and 3, indicating astrocytoma, IDH-mutant), and group 3 (risk subgroups 4 and 5, indicating glioblastoma, IDHwt). The progression-free survival (PFS) and overall survival (OS) were estimated for each imaging risk group, subgroup, and pathological diagnosis. Time-dependent area-under-the receiver operating characteristic analysis (AUC) was used to compare the performance between imaging-based and pathology-based survival model. Results: Both OS and PFS were stratified according to the five imaging-based risk subgroups (P < 0.001) and three imaging-based risk groups (P < 0.001). The three imaging-based groups showed high performance in predicting PFS at one-year (AUC, 0.787) and five-years (AUC, 0.823), which was similar to that of the pathology-based prediction of PFS (AUC of 0.785 and 0.837). Combined with clinical predictors, the performance of the imaging-based survival model for 1- and 3-year PFS (AUC 0.813 and 0.921) was similar to that of the pathology-based survival model (AUC 0.839 and 0.889). Conclusion: Imaging-based survival stratification according to the 2021 WHO classification demonstrated a performance similar to that of pathology-based survival stratification, especially in predicting PFS.

• International Journal of Stem Cells
• /
• 제16권3호
• /
• pp.281-292
• /
• 2023

Background and Objectives: Human induced pluripotent stem cell (hiPSC)-derived cardiomyocyte (CM) hold great promise as a cellular source of CM for cardiac function restoration in ischemic heart disease. However, the use of animal-derived xenogeneic substances during the biomanufacturing of hiPSC-CM can induce inadvertent immune responses or chronic inflammation, followed by tumorigenicity. In this study, we aimed to reveal the effects of xenogeneic substances on the functional properties and potential immunogenicity of hiPSC-CM during differentiation, demonstrating the quality and safety of hiPSC-based cell therapy. Methods and Results: We successfully generated hiPSC-CM in the presence and absence of xenogeneic substances (xeno-containing (XC) and xeno-free (XF) conditions, respectively), and compared their characteristics, including the contractile functions and glycan profiles. Compared to XC-hiPSC-CM, XF-hiPSC-CM showed early onset of myocyte contractile beating and maturation, with a high expression of cardiac lineage-specific genes (ACTC1, TNNT2, and RYR2) by using MEA and RT-qPCR. We quantified N-glycolylneuraminic acid (Neu5Gc), a xenogeneic sialic acid, in hiPSC-CM using an indirect enzyme-linked immunosorbent assay and liquid chromatography-multiple reaction monitoring-mass spectrometry. Neu5Gc was incorporated into the glycans of hiPSC-CM during xeno-containing differentiation, whereas it was barely detected in XF-hiPSC-CM. Conclusions: To the best of our knowledge, this is the first study to show that the electrophysiological function and glycan profiles of hiPSC-CM can be affected by the presence of xenogeneic substances during their differentiation and maturation. To ensure quality control and safety in hiPSC-based cell therapy, xenogeneic substances should be excluded from the biomanufacturing process.

• Journal of Microbiology and Biotechnology
• /
• 제34권4호
• /
• pp.920-929
• /
• 2024

As a pivotal defensive line against multitudinous malignant tumors, natural killer (NK) cells exist in the tumor microenvironment (TME). RAD18 E3 Ubiquitin Protein Ligase (RAD18) has been reported to foster the malignant progression of multiple cancers, but its effect on NK function has not been mined. Here, the study was designed to mine the mechanism by which RAD18 regulates the killing effect of NK cells on colorectal cancer (CRC) cells. Expression of E2F Transcription Factor 7 (E2F7) and RAD18 in CRC tissues, their correlation, binding sites, and RAD18 enrichment pathway were analyzed by bioinformatics. Expression of E2F7 and RAD18 in cells was assayed by qRT-PCR and western blot. Dual-luciferase assay and chromatin immunoprecipitation (ChIP) assay verified the regulatory relationship between E2F7 and RAD18. CCK-8 assay was utilized to assay cell viability, colony formation assay to detect cell proliferation, lactate dehydrogenase (LDH) test to assay NK cell cytotoxicity, ELISA to assay levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), and immunofluorescence to detect expression of toxic molecules perforin and granzyme B. High expression of RAD18 and E2F7 was found in CRC tissues and cells. Silencing RAD18 could hamper the proliferation of CRC cells, foster viability and cytotoxicity of NK cells, and increase the secretion of GM-CSF, TNF-α, IFN-γ as well as the expression of perforin and granzyme B. Additionally, ChIP and dual-luciferase reporter assay ascertained the binding relationship between RAD18 promoter region and E2F7. E2F7 could activate the transcription of RAD18, and silencing RAD18 reversed the inhibitory effect of E2F7 overexpression on NK cell killing. This work clarified the inhibitory effect of the E2F7/RAD18 axis on NK cell killing in CRC, and proffered a new direction for immunotherapy of CRC in targeted immune microenvironment.

• 한국산림과학회지
• /
• 제98권4호
• /
• pp.496-503
• /
• 2009

간척지는 높은 토양 염도와 지하수위로 인해 수목의 생육에 적합하지 않은 환경으로 알려져있다. 그러나 과거 농경지로 사용되던 간척지가 최근 들어 산업단지, 주거단지, 또는 공원으로 전환되면서 이 지역에 수목 식재의 필요성이 높아지고 있다. 본 연구는 농경지에서 다른 용도로 전환되는 지역 중 하나인 충남 서산 간척지에 도입할 수 있는 내염성 수종을 선택하기 위하여 수행되었다. 서로 다른 복토 재료(준설토, 준설토+산토)와 복토 높이(1.5 m, 2.0 m)를 이용하여 시험 포지를 조성한 후 8개 수종-소나무(Pinus densiflora), 곰솔(Pinus thunbergii), 메타세콰이어(Metasequoia glyptostroboides), 이팝나무(Chionanthus retusus), 산딸나무(Cornus kousa), 산벚나무(Prunus sargentii), 상수리나무(Quercus acutissima), 느티나무(Zelkova serrata)-의 묘목을 대상으로 2006년 4월에서 2008년 11월까지 매년 고사율과 수목 생장을 조사하였다. 3년간 묘목의 생존율은 수종 간 유의한 차이를 보였다(F=9.632, P<0.001). 산딸나무, 상수리나무, 산벚나무는 50% 이상의 상대적으로 높은 고사율을 보였으며, 해송, 메타세콰이어, 느티나무가 각각 3년 평균 고사율 6.4%, 2.3%, 24.7%로 상대적으로 생존율이 높았다. 묘목의 수고 생장 역시 수종간 유의한 차이를 보였다(F=4.749, P=0.002). 메타세콰이어와 느티나무가 절대생장과 식재 당시 대비 상대 생장 모두를 고려했을 때 상대적으로 높은 수고 생장을 보였다. 대부분의 수종들이 이식 다음해에는 낮은 생장을 보였으며, 이식 2년 후부터 생장이 회복되었다. 수종에 관계없이 복토 높이가 높을수록, 준설토보다는 산림토양을 복토하였을 때에 수목의 생존율과 생장이 높게 나타났다. 해송, 메타세콰이어, 느티나무가 타 수종에 비해 높은 생존율과 생장을 보여 이 지역에 상대적으로 적합한 수종으로 나타났으며, 이팝나무, 산딸나무와 산벚나무는 이 지역에서 적응력이 떨어지는 것으로 판단되었다.

• 한국응용과학기술학회지
• /
• 제34권3호
• /
• pp.568-576
• /
• 2017

최근 기능성과 친환경 화장품에 대한 관심이 증가하고 있으며, 이에 따라 안전하면서 효능이 우수한 식물 추출물을 활용한 소재 개발이 이루어지고 있는 실정이다. 따라서 본 연구에서도 주로 건강 기능성 소재로써 다양한 효능이 있는 것으로 알려진 그라비올라 추출물이 기능성 화장품 소재로써의 가능성을 확인하고자 하였다. 그라비올라 추출물의 항산화 활성을 확인하고자 총 폴리페놀과 총 플라보노이드 함량, DPPH radical 소거 활성을 측정하였고, HDF 세포에서의 세포 독성을 확인한 후 적정 농도에서 HDF 세포에 과산화수소($H_2O_2$)를 처리하여 산화적 스트레스에 대한 ROS 활성 억제 효과와 세포 보호 효과를 측정하였다. 본 실험 결과, 그라비올라 추출물은 항산화 지표가 되는 총 폴리페놀과 플라보노이드의 100g당 26.6 mg(CA)/100g, 14.3 mg(CA)/100g의 높은 함량을 확인하였으며, 높은 radical 소거 활성을 확인하였다. HDF 세포에 대한 세포 생존율을 측정한 결과, 모든 농도에서 유의한 세포 독성이 나타나지 않았으며, 추후 $100{\mu}g/mL$ 농도에서 실험하였다. $H_2O_2$로 유도된 HDF 세포에 ROS 활성 억제를 측정한 결과, 농도 의존적인 ROS 활성 억제 효과를 확인하였고, $H_2O_2$를 4 시간, 24 시간, 48 시간 동안 처리 후 그라비올라 추출물의 세포 보호 효과를 측정한 결과, $25{\mu}g/mL$ 농도에서 24시간까지 89.92%의 높은 세포 보호 효과를 확인하였다. 이와 같은 결과를 통하여 그라비올라 추출물은 항산화 활성이 우수하고, HDF 세포에 대한 독성이 거의 없으며, $H_2O_2$에 의해 발생하는 활성산소에 대한 효과적인 활성 억제 효과와 세포 보호 효과가 우수한 것으로 확인됨에 따라 항산화 및 세포 보호 효과를 가진 다양한 기능성 소재로서의 가능성을 확인하였다.