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QTL Mapping for 6-Year-Old Growths of a Single Open-Pollinated Half-Sib Family of a Selected Clone 7-1037 in Loblolly Pine(Pinus taeda) and Average Effect of QTL Allele Substitution (테다소나무 7-1037 클론의 단일 반형매 풍매가계 6년생 생장에 대한 QTL mapping과 QTL 대립유전자 치환의 평균효과)

  • Kim, Yong-Yul;Lee, Bong-Choon;O'Malley, David M.
    • Journal of Korean Society of Forest Science
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    • v.95 no.4
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    • pp.483-494
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    • 2006
  • We conducted QTL mapping for 6-year growths of open-pollinated half-sib progenies from a selected clone 7-1037 in Pinus taeda. With an AFLP marker analysis on haploid DNA samples from the megagametophytes of the open-pollinated seeds, we constructed 20 framework maps spanning a total of 1,869 cM in total length and 18.5 cM in an average interval length between markers. Composite interval mapping reveals that one QTL explains 5.9% of the total phenotypic variation of height, and three QTLs account for 3.9~5.6% of the variation of diameter at breast height (DBH). There are no correlations between the QTLs. The genetic effects of the QTLs are 39.6 cm in height and 7.20~9.41 mm in DBH, respectively, The average effects of gene substitution of the markers closely linked with the QTLs are 44.3 cm in height and 8.38~11.81 m in DBH. Under an assumption that the within-family heritability for the growth traits of loblolly pine is less than 0.2, the QTLs account for 26.8% of the additive genetic variance of the progenies. In terms of relative selection efficiency, the individual selection based on QTL markers could be 5 times as high as phenotypic selection. The results in this study indicate that the QTL mapping method with open-pollinated half-sib family could be more practical and applicable to the conventional seed orchard-based selection work than other mapping methods with a single full-sib family, in particular from the viewpoint that it can provide crucial information for within-family individual selection such as breeding value.

Studies on the Lipids of Shiitake Mushroom, Lentinus edodes (표고버섯의 지질(脂質)에 관(關)한 연구(硏究))

  • Tsuyuki, Hideo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.4
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    • pp.419-427
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    • 1985
  • The properties of lipids in cap and stalk of 'Koshin' (Shiitake, Lentinus edodes, withxpanded cap) were studied. It was also carried out that the characteristics of lipids in fresh 'Donko'(unexpanded cap) and the changes of lipids in those samples by various drying process. The total lipid (TL) content in the cap and stalk of 'Koshin' were 4.58% and 2.65% respectively. It was found that the contents of neutral lipids (NL, $46.7{\sim}48.8%$) and phospholipids (PL, $42.0{\sim}43.8%$) were high, while that of glycolipids(GL, $9.2{\sim}9.5%$) was low. The main lipid in NL was triacylglycerol (TG, $58.0{\sim}58.5%$) followed by sterolester (SE, $16.9{\sim}17.4%$), sterol (ST, $15.6{\sim}16.4%$), diacylglycerol (DG, $5.8{\sim}6.0%$) and monoacylglcerol (MG, $1.1{\sim}1.3%$). The main lipids in PL was phosphatidyl ethanolamine (PE, $58.3{\sim}6.02%$) followed by phosphatidyl choline (PC, $17.3{\sim}19.5%$), cardiolipin (CA, $12.8{\sim}14.0%$) and lyso-phophatidyl choline (LPD, $4.8{\sim}6.6%$). Fatty acid composition was significantly different among TL, NL, GL and PL contained in cap and stalk. The main fatty acids was $C_{18:2}$ followed by $C_{16:0}$ and $C_{18:1}$ acids. The TL contents in cap and stalk of fresh 'Donko' were 3.7% and 2.5%. The changes of TL contents, PoV by drying were not almost observed. AV(acid value) of TL in the sun dried samples was a little lower than those of the fresh and hot air dried samples, on the contrary NL content in TL of the former was a little higher than those of the latters. The main lipid in NL of the three samples was TG followed by SE, ST, DG, MG and free fatty acid (FFA). In the above lipids, FFA contents in NL of the sun dried sample was a little higher than those of the fresh and hot air dried samples, but TG content in NL of former was a little lower than those of the latters. The main lipid in GL of the three samples was digalactosydiglycerol (DGDG) followed by acylsterylglucoside(ASG), sterylglucoside (SG), monogalactosyldiglycerol (MGD) and cerebroside (CER). Influence of drying on these lipid content were nol almost recognized. The main lipid in PL of the three samples was PE followed by PC, CA, LPC and phosphat idyl serine (PS). In the above lipids, PE and PC contents in PL of the sun dried one were a little lower than those of the fresh and hot air dried ones. The main fatty acid in TL, NL, GL and PL of the three samples was $C_{18:2}$ followed by $C_{16:0},\;C_{18:1}$. The $C_{18:2}$ acid contents of the sun dried sample was a little lower than those of the fresh and hot air dried ones, while $C_{16:0}$ content of the former was a little higher than those of the former was a little lower than those of the latters.

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The Study for the Development of Physical Standard and State of Nutrition of a Deaf & Dumb School Boys & Girls (농아학원생(聾啞學院生)의 영양상태(營養狀態)와 체위발달도(體位發達度)에 관(關)한 연구(硏究))

  • Lee, Geum-Yeong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.5 no.1
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    • pp.87-92
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    • 1976
  • Comparing the amount of Hb, the nutriture of the deaf-mutes are better than that of the amentias and the nursery school children. The former, however, shows less condition than that of the boys and girls in the junior physical education school (Hb : 12.7) and the normal family children (Hb : 10.8). In the group of six to eleven years old that is a class a elementary school, the physical condition such as stature, chest circumference, and weight of the deaf -mute is respectively almost the same degree, which is above standard in our country. On the contrary the degree of the amentias and the nursery school children has an inferiority approaching to the standard. In the group of twelve to fifteen years old that is a class of junior high school, the nursery school children have the lowest physical condition and the boys and girls of the physical training school the highest. The order of the growth of physical condition is as follows : Nursery school children

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A Study to Determine the Effectsiveness of Severance Hospice Home Care Program (호스피스케어에 대한 평가 연구 - 세브란스호스피스 중심으로)

  • Kingsley, Marian R.N.;Cho, Won-Jung;Kim, Cho-Ja;Lee, Won-Hee;Yoo, Ji-Soo
    • The Korean Nurse
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    • v.29 no.4
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    • pp.51-72
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    • 1990
  • The purpose of this study was to determine whether Severance Hospice Home Care Pro gram was able to meet its objectives. This was done in order to show in detail the effects of hospice home care on the quality of life of terminally ill patients and to provide rationale for setting up more hospice home care programs in korea. The results of the study were as follows: The subjects of the study were 100 terminally ill patients who hnd died 'while in the hospice program and 64 family members who were registered with Severance Hospice Home Care Program between march 1988 and Feb. 1990. The nursing needs of these terminally ill patients were assessed by the nursing records of these patients. The need for pain control(82%) was the highest nursing need so far as the physical aspects were concerned. This was followed by poor appetite(37%), 8 dyspnea(34%), nausea and vomiting(30%) in that order of frequency. In reqard to spiritual needs, the need for religious' support was also high at 72%. Their main psychological symptoms were anxiety and fear(34% ). Burn-out was a major problem for 44% of the family members. The psychological process experiencel by the terminal ill patients was compared to the dying process, described by Kiibler Ross. In comparison of the five stages outlined by kubler Ross with the dying process of the subjects it was found that the subjects not only experienced the five stages but also experienced denial and doubt-fulness or denial with acceptance or acceptance with the expectation of a miracle. But rather than acceptance of the dying process, giving up was a frequent end point of the psychological process, of the subjects. However, when the combination of states was observed, most of the patients reached the state of acceptance in the dying process. It was difficult to identify a definite pattern of change in the psychological process of the subjects. Also it was difficult to identify the factors that influenced the psychological process. The symptoms of the terminally j]] subjects just before dying, that is, 3-4 days before dying included apparent signs of dying. These were a reduction of intake(77%), reduction of the amount of urination(63%), increase in sleeping time (64%) and acceptance of dying by patients and their families who had been unaccepting be before that time(66%). The primary care givers(family member's) degree of satisfaction with the care given to the patient by the hospice was 88.7%. The results of this study show that Severance Hospice Home Care Program had a positie effeet on the quality of life of the terminally ill patients and their family members as they faced the death of the patient. It can be seen from this study that there is an urgent need to extend hospice programs - in order to provide quality of care for terminally ill patient and their families. Based upon the reesults of this study several suggestions are presente as follows: 1) A follow up study should be carried out to identify the dying process as it is unique to Korea. 2) A comparison should be made of other hospice care programs. 3) A comparison study should- be made with subjects who do not receive any hospice care as compared to those who do by use of an experimental and control group methodology. 4) There is a need to determine a scientific method to adequeto measure the interventions carried out to meet the hospice patients nursing care needs. 5) A study should be made using quality research methodology to evaluate effects of hospiec care from the patients, their family members and the nurrse's perspective.

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Biochemical and Nutritional Effects of Rice, Barley, and Millet on Rats (백미대맥(白米大麥) 및 속(粟) 사료(飼料)가 실험용(實驗用) 백서(白鼠)에 미치는 영양학적(營養學的) 및 생물화학적(生物化學的) 연구(硏究))

  • Haw, Kum
    • Journal of Nutrition and Health
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    • v.1 no.1
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    • pp.9-18
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    • 1968
  • Dividing into six(6) experimental groups; Stock Diet(SD) Group, Rice Diet(RD) Group, Rice Diet supplemented with Barley(RD-BD) Group, Barley Diet(BD) Group, Rice Diet supplemented with Millet(RD-MD) Group, and Millet Diet(MD) Group, biochemical and nutritional effects of rice, barley, and millet on rats are studied. The grains used in this experiment are polished ones and purchased from market. Six(6) weaning male albino rats are housed into the individual cages in each group and fed with each experimental diet and water ad libitum for 21 weeks. The composition of the experimental diets are shown in Table 1. At the end of experiment, the appearences of rats are observed and the bloods are sampled by heart puncture method following ether anesthesia for determinations of serum protein and serum cholesterol. And the livers are sampled immediately right after heart after heart puncture for determinations of liver weight, liver nitrogen, liver fat, and liver glycogen. And also liver tissues are observed histologically. The result of growth experiment is shown in Table 2 and Figure 7. The results of blood analysis are shown in Table 3 and the results of liver analysis are shown in Table 4. And the histological observations of liver tissue are shown in Figures 1, 2, 3, 4, 5, and 6. According to the above results, it could be summarized as follows; 1) The normal appearences are observed at SD Group and BD Group. Alopecia is observed at RD, RD-­BD, RD-MD, and MD groups and the wet appearences of hair are observed at RD-MD and MD groups. The color of hair is changed to yellowish white at RD, RD-MD groups. The maximum growth inhibition, is observed at MD group and the growths of RD, RD-BD, BD, and RD-MD groups are significantly lower than that of SD group. The growth of RD group is lower than those of RD-BD and BD groups, though this difference is not significant. 2) At the blood analysis, the highest serum protein content is shown at SD group and the lowest serum protein is observed at MD group. The serum cholesterol contents are not significantly different among the six(6) experimental groups. 3) The highest liver weight is observed at SD group and the lowest weight is shown at MD group. However, oppositely the highest liver weight per 100 grams of body weight at MD group and the lowest liver weight per 100 grams of body weight at SD group are observed as shown in Table 6. 4) The differences of liver nitrogen contents of RD, RB-BD, BD, RD-MD, and MD Groups are not significant except SD group showing the highest content. 5) The significant highest content of liver fat is observed at RD group and the lowest content of liver fat is shown at SD group. And no significant differences in contents of liver glycogen are observed among the six(6) experimental groups. 6) No significant abnormal histological changes of liver tissue are observed at SD, RD-BD, and BD groups. However, the fat metamorphosis at peripheral zone of liver tissue is observed at RD Group and the some histological abnormal changes are observed at RD-MD and MD groups. According to the above summaries, the followings might be estimated and recommended; 1) The BD group shows histological normality of liver tissue, improvement of liver fat and liver nitrogen, normality of appearence, and better growth compared with RD Group. This could be indicated that barley is nutritionally and biochemically better than rice as the feeding diet on rat. And it might be considered that there might be the species speciality for nutritionary evaluation of food. 2) Recently, Marshall indicated that the high consumption of carbohydrate causes fatty liver and Kondo reported that the higher consumption of rice causes the more heart disease and the shorter life span. Considering these reports, the higher consumption of rice as a main dish in our country should be considered as an important national nutritional problem. And this might be improved by supplementing with barley or other grains to rice.

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Dietary behaviors of female marriage immigrants residing in Gwangju, Korea (광주지역에 거주하는 결혼이주 여성의 식생활 조사)

  • Yang, Eun Ju
    • Journal of Nutrition and Health
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    • v.49 no.3
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    • pp.179-188
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    • 2016
  • Purpose: This cross-sectional study aimed to document the dietary behaviors, dietary changes, and health status of female marriage immigrants residing in Gwangju, Korea. Methods: The survey included 92 female immigrants attending Korean language class at a multi-cultural family support center. General characteristics, health status, anthropometric data, dietary behaviors, and dietary changes were collected. Results: Mean age of subjects was 31.3 years, and home countries of subjects were Vietnam (50.0%), China (26.0%), Philippines (12.0%), and others (12.0%). Frequently reported chronic diseases were digestive diseases (13.2%), anemia (12.1%), and neuropsychiatry disorder (8.9%). Seventeen percent of the subjects was obese ($BMI{\geq}25kg/m^2$). Dietary score by Mini Dietary Assessment was 3.45 out of 5 points. Dietary scores for dairy foods, meat/fish/egg/bean intake, meal regularity, and food variety were low, and those for fried foods and high fat meat intake were also low. Thirty-three percent of subjects answered that they have changed their diet and increased their consumption of fruits and vegetables after immigration. Length of residence in Korea was positively associated with BMI and waist circumference. Length of residence tends to be positively associated with dietary changes and obesity as well as inversely associated with disease prevalence. Conclusion: The study shows that length of residence is inversely related to disease prevalence. However, this association is thought to be due to the relatively short period of residence in Korea and thus the transitional phase to adapting to dietary practices. As the length of residence increases, disease patterns related to obesity are subject to change. Healthy dietary behaviors and adaptation to dietary practices in Korea in female marriage immigrants will not only benefit individuals but also their families and social structure. Therefore, varied, long-term, and target-specific studies on female marriage immigrants are highly needed.

The Development and Significance of Physic Gardens in the Late Goryeo and Early Joseon Dynasties (여말선초 약초원의 형성 과정과 조경사적 의미 고찰)

  • Kim, Jung-Hwa
    • Journal of the Korean Institute of Landscape Architecture
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    • v.45 no.5
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    • pp.60-70
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    • 2017
  • This study traces the development of physic gardens in Korea and explores their significance in the history of landscape architecture. For this purpose, records related to physic gardens from medical sources from the period of the Three States to the Joseon dynasty, when herbal medicine was systematized as a field, were searched. Physic gardens had been developed by the time of the late Goryeo and early Joseon dynasties, in the 13th and the 15th centuries. Yakpo(kitchen gardens for medicinal herbs) were cultivated by a group of new high-level officials in the late Goryeo dynasty, when an increasing interest in hyangyak(native herbs) emerged under the influence of the Neo-Confucian perspective on nature, which emphasized locality. The sources analyzed in this study confirm that physic gardens called jong-yakjeon(royal medicinal herb gardens) were in operation in the early Joseon dynasty when policies to investigate, discover, cultivate, and research native herbs were put into place. It is likely that the jong-yakjeon were established at the beginning of the Joseon dynasty as subsidiary facilities under its central medical institutions, the Naeuiwon and Hyeminseo, and then declined in the late Joseon dynasty. Jong-yakjeon can be confirmed to have existed in the mid-15th century. Physic gardens were located in several places outside the Fortress Wall of Hanyang, such as Yakhyeon, Yuldo, Yeoudo, and Saari. The total area encompassed by physic gardens was about 160,000 square meters in the early 18th century. In jong-yakjeon, dozens of medicinal herbs were cultivated, including Schizonepeta tenuifolia var. japonica, Rehmannia glutinosa, and Glycyrrhiza uralensis Fischer, and these gardens were operated by physicians dispatched from the Naeuiwon and dozens of provincial slaves. In conclusion, the jong-yakjeon were similar to the physic gardens of Renaissance medical universities in that they reflected the interest in and development of theories about new herbs, and were similar to the physic gardens of medieval castles and monasteries in terms of species types, location, and function. This paper has limitations in that it does not present the specific spatial forms of the yakpo or the jong-yakjeon. Nevertheless, this paper is significant for the field of garden history because it shows that physic gardens in Korea appeared in the late Goryeo and early Joseon dynasties concomitantly with the development of medicine towards native herbs and functioned as utilitarian gardens to cultivate community remedies.

Simulation and Measurement of Signal Intensity for Various Tissues near Bone Interface in 2D and 3D Neurological MR Images (2차원과 3차원 신경계 자기공명영상에서 뼈 주위에 있는 여러 조직의 신호세기 계산 및 측정)

  • Yoo, Done-Sik
    • Progress in Medical Physics
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    • v.10 no.1
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    • pp.33-40
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    • 1999
  • Purpose: To simulate and measure the signal intensity of various tissues near bone interface in 2D and 3D neurological MR images. Materials and Methods: In neurological proton density (PD) weighted images, every component in the head including cerebrospinal fluid (CSF), muscle and scalp, with the exception of bone, are visualised. It is possible to acquire images in 2D or 3D. A 2D fast spin-echo (FSE) sequence is chosen for the 2D acquisition and a 3D gradient-echo (GE) sequence is chosen for the 3D acquisition. To find out the signal intensities of CSF, muscle and fat (or scalp) for the 2D spin-echo(SE) and 3D gradient-echo (GE) imaging sequences, the theoretical signal intensities for 2D SE and 3D GE were calculated. For the 2D fast spin-echo (FSE) sequence, to produce the PD weighted image, long TR (4000 ms) and short TE$_{eff}$ (22 ms) were employed. For the 3D GE sequence, low flip angle (8$^{\circ}$) with short TR (35 ms) and short TE (3 ms) was used to produce the PD weighted contrast. Results: The 2D FSE sequence has CSF, muscle and scalp with superior image contrast and SNR of 39 - 57 while the 3D GE sequence has CSF, muscle and scalp with broadly similar image contrast and SNR of 26 - 33. SNR in the FSE image were better than those in the GE image and the skull edges appeared very clearly in the FSE image due to the edge enhancement effect in the FSE sequence. Furthermore, the contrast between CSF, muscle and scalp in the 2D FSE image was significantly better than in the 3D GE image, due to the strong signal intensities (or SNR) from CSF, muscle and scalp and enhanced edges of CSF. Conclusion: The signal intensity of various tissues near bone interface in neurological MR images has been simulated and measured. Both the simulation and imaging of the 2D SE and 3D GE sequences have CSF, fat and muscle with broadly similar image intensity and SNR's and have succeeded in getting all tissues about the same signal. However, in the 2D FSE sequence, image contrast between CSF, muscle and scalp was good and SNR was relatively high, imaging time was relatively short.

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Morphological and Cultural Characteristics of Fungi Causing Rice Sclerotial Diseases (수도균핵병(水稻菌核病)을 일으키는 진균(眞菌)의 형태적(形態的) 및 배양적(培養的) 특성(特性))

  • Kim, Wan-Gyu
    • The Korean Journal of Mycology
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    • v.21 no.1
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    • pp.16-22
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    • 1993
  • Morphological and cultural characteristics of fungi causing rice sclerotial diseases were examined. Hyphal widths of R. solani and R. oryzae were same and ranged $6.0-12.0\;{\mu}m$ with average $9.0\;{\mu}m$, the widest among those of the sclerotial fungi examined. Hyphal width of R. oryzae sativae ranged $6.0-9.0{\mu}m$ with average $7.4{\mu}m$. Hyphal width of R. cerealis was the narrowest among those of Rhizoctonia species examined, and the same was hyphal width of S. oryzae among those of Sclerotium species. Nuclear staining by HCL-Giemsa method showed that R. solani and R. oryzae had many nuclei within one hyphal cell, S. oryzae one nucleus, and the other sclerotial fungi mostly two nuclei. The nuclear number of R. solani was the largest, which ranged 2-17 with average 6.3. Average size of sclerotia of the sclerotial fungi except S. hydrophilum and S. oryzae produced in lesions ranged 1.0-2.0mm. Average size of sclerotia of S. hydrophilum and S. oryzae was 0.5mm and 0.24mm, respectively. Sclerotia of R. solani and R. oryzae produced in culture were more variable in size and larger than those produced in lesions. However, the sclerotial sizes of the other sclerotial fungi produced in culture were almost the same as those produced in lesions. Sclerotial colors of sclerotial fungi produced in lesions were similar to those produced in culture, but sclerotial shapes of some sclerotial fungi exhibited somewhat difference between the sclerotia produced in lesions and in culture. Optimum temperature for mycelial growth of R. cerealis was $23^{\circ}C$, and that of the other sclerotial fungi ranged from $27\;to\;33^{\circ}C$. Maximum temperature for mycelial growth of some sclerotial fungi was as high as $41^{\circ}C$, while that of R. cerealis was as low as $31^{\circ}C$. Minimum temperature for mycelial growth of R. cerealis was $2^{\circ}C$, and that of the other sclerotial fungi ranged from $6\;to\;10^{\circ}C$.

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Features and Component Analysis of the GeumguJagi(金釦瓷器) Excavated from Seongneung(石陵) (석릉(碩陵) 출토 금구자기(金釦瓷器)의 특징과 성분 분석)

  • Sung, Kiyeol
    • Korean Journal of Heritage: History & Science
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    • v.53 no.1
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    • pp.150-167
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    • 2020
  • The purpose of this paper is to share the status and introduce a scientific analysis of the GeumguJagi (gilt-silver overlay porcelain, 金釦瓷器) excavated from Seongneung (石陵). This analysis aimed to highlight the GeumguJagi excavated from the Royal Tombs of Goryeo with a clear lower year (1237) and to aid research into the GeumguJagi. In 2001, the National Institute of Cultural Heritage excavated and investigated Seongneung in Heejong (熙宗). Various artifacts such as celadon, gold, bronze, and iron products were collected from the chambers inside of the tomb. There were a total of 160 celadon items including bowls, dishes, glasses, and saucers. Of those, there were 58 celadon items (including fragments) with metal frames on the openings. These consisted of bowls, plates, lids, and saucers. Until recently, in various exhibitions and papers, only one GeumguJagi was known to have been excavated from Seongneung, which was a . However, the survey identified a number of further GeumguJagis. It had been understood from inherited and excavated products that the materials used for ornaments were restricted to high-quality celadon. However, this study confirmed that the excavation of Seongneung demonstrated the use of various other materials for different models and qualities of GeumguJagis. It can be said that it is characteristic that various models and quality are confirmed together through the excavation of Seongneung. A scientific analysis was carried out that selected 12 of 58 products excavated from Seongneung. Results showed that the main component used for Geumgu ornaments was tin (Sn), and trace amounts of copper (Cu) and lead (Pb) were also commonly identified. When analyzing the material used to affix the metal fittings, this was found to be glue (膠) made from animal skins, muscle, and bones. This pattern matches that of the GeumguJagi excavated from Paju Hyeeumwonji, and the reason for this could be assumed on the basis of the contents of the 『Cheongonggaemul (天工開物)』 written by Song Ongsung (宋應星) during the Ming Dynasty. At that time, metals such as tin and copper would have been difficult to obtain. 『Xuānhwafengshi Gaolitujing (宣和奉使高麗圖經)』 shows that the use of metal was limited to certain classes; thus, the use of the GeumguJagi seems to have been centered around the royal family.