• Natural Product Sciences
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• v.18 no.2
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• pp.137-140
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• 2012

The aim of this research was to investigate the industrial application of Zanthoxylum schinifolium. Antibacterial activities of the n-hexane, methylene chloride (MC), ethyl acetate, and butanol fractions of Z. schinifolium were tested against Escherichia coli, Staphylococcus aureus and Helicobacter pylori. Among the fractions, the n-hexane and MC fractions showed the stronger antibacterial activity against H. pylori, with an inhibition zone greater than 10 mm in disc assays. Further testing of bergapten and lupeol from the MC fraction of Z. schinifolium revealed the antibacterial effects against E. coli, S. aureus and H. pylori, suggesting their potential use as antibacterial agents.

• Journal of the Korean Wood Science and Technology
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• v.37 no.5
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• pp.474-479
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• 2009

Pinus koraiensis inner bark was collected and extracted with 95% ethanol. The extracts were concentrated and then sequentially fractionated using n-hexane, $CH_2Cl_2$, EtOAc, and $H_2O$ to be freeze dried. A portion of EtOAc fraction (6.6 g) was chromatographed on a Sephadex LH-20 column using aqueous methanol to isolate (+)-catechin (1), (-)-epicatechin (2), and trans-pinostilbenoside (3). Resveratrol (4) and trans-pinostilbene (5) were isolated by column chromatography using EtOH-hexane mixture after purification with aqueous methanol. The structures of these stilbenosides and flavans were characterized by spectroscopic tools using NMR and MS.

• Journal of the Korean Wood Science and Technology
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• v.14 no.3
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• pp.30-35
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• 1986

To elucidate chemical structure of the wood extracitives, softwood Larix leplolepis Gorden) metal was extracted with 95% ethanol at room temperature for 72 hours. The extract was fractionated with organic solvents such as n-hexane, chloroform. ether, and ethylacetate. From the n-hexane soluble fraction of the extratives, flavonoid and sterol compounds were isolated and identified as taxifolin(5,7,3',4'-tetrahydroxyflavanonol)(I) and ${\beta}$-sitosterol(II) by UV, IR, $^1$H-NMR spectroscopy and Mass spectrometry.

• Journal of the Korean Wood Science and Technology
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• v.13 no.4
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• pp.58-66
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• 1985

In order to elucidate chemical structure of the wood extractives, softwood (Pinus koraiensis Sieb. et Zucc.) meal was extracted with 95% ethanol at room temperature for 72 hours. The extract was fractionated with organic solvents such as n-hexane, ether, ethylacetate, and acetone. From the n-hexane and ether soluble fraction of the wood extractives, four flavonoid compounds were isolated and identified as 5-hydroxy-7-methoxyflavone (I), 5-methoxy-7-hydroxyflavone (II), 5-hydroxy-7-methoxyflavanone (III), and 5-methoxy-7-hydroxyflavanone (IV) by UV, IR, $^1$H-NMR spectroscopy and MS spectrometry.

• Korean journal of food and cookery science
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• v.27 no.5
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• pp.523-530
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• 2011

This study was conducted to investigate antimicrobial, antioxidant and anticancer activities of Opuntia humifusa (OH) petal extracts. The methanol and hexane extracts of OH petals showed their highest antimicrobial activity against Clostridium perfringens. The OH petal butanol fraction had the best antioxidative peroxynitrite scavenging activity among OH petal extracts. The DPPH scavenging activity of OH petals was lower than the peroxynitrite scavenging effect. The hexane and methanol fractions at a concentration of 200 ${\mu}g$/mL inhibited proliferation >80% in four kinds of human cervical cancer cells(B16F10, HepG2, HT-29 and MCF-7). In particular, the anticancer effect against B16F10 human skin cancer cells at the same concentration was higher than that in the other cancer cells.

• Journal of the Korean Wood Science and Technology
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• v.26 no.4
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• pp.71-77
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• 1998

To elucidate chemical structure of the wood extractive, wood meal of Larix leptolepis Gorden was extracted with 95% ethanol for 72 hours. The extract was fractionated with organic solvents such as n-hexane, chloroform, diethylether, and ethyl acetate. From the hexane somble fraction of the extractives, a new diterpene compound was isolated and identified as 6-acetoxymanool or 13-hydroxy-8(17), 14-labddienyl-6-acetate by IR, $^1H(^{13}C)$-NMR and Mass spectrometry.

• Journal of Life Science
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• v.19 no.1
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• pp.117-122
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• 2009

This study was conducted to investigate the antioxidant effect of 80% ethanol extracts from Angelica acutiloba Kitagawa (A. acutiloba Kitagawa) in vitro. The extract was further fractionated subsequently by n-hexane, chloroform, ethylacetate, n-butanol and water. Antioxidative activities of different fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical generation, Rancimat test, thiobarbituric acid (TBA) value, nitrite scavenging activity, inhibition of lipid peroxidation and peroxide value (POV) in linoleic acid in comparison with the commercial antioxidant butylated hydroxy toluene (BHT). Antioxidant activities of n-hexane fraction of Angelico acutiloba Kitagawa ethanol extract were the highest among fractions and were a little less than that of BHT. Nitrite scavenging activity showed the most remarkable effect at pH 12. These results suggest that ethanol extracts of A. acutiloba Kitagawa can be used in natural antioxidant source.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.9
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• pp.1220-1225
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• 2012

Ligularia stenocephala leaves were subjected to extraction by using several solvents with different polarities, which were then investigated for their antioxidant and antimicrobial activities. Extraction yield of ethanol extract of Ligularia stenocephala was 21.36%. The water fraction showed the highest extraction yield of 60.35%, followed by the n-butanol, n-hexane, and ethyl acetate fractions. Total polyphenolic content was the highest (254.00 mg/g GAE) in the ethyl acetate fraction of Ligularia stenocephala extract. The ethyl acetate fraction had $IC_{50}$ values of 0.28 mg/mL for DPPH radical scavenging, and 96.$67{\pm}0.09%$ for ABTS radical scavenging activity. Further, FRAP value was significantly higher in the ethyl acetate fraction. The ethyl acetate fraction showed antimicrobial activities against B. cereus, and the diameter of the zone of inhibition was 10.2 mm at 5 mg/disc. These results suggest that the ethyl acetate fraction of Ligularia stenocephala possesses the antimicrobial activities against B. cereus, and its high antioxidant activity could be applicable to food additives and as a natural cosmetic ingredient.

• Microbiology and Biotechnology Letters
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• v.38 no.1
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• pp.105-111
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• 2010

Caesalpinia sappan L. has long been commonly used in oriental folk medicines to treat diseases. To investigate the antibacterial effects from C. sappan L. heart wood, the MeOH soluble extract was successively fractionated by using hexane, $CHC1_3$, EtOAc, BuOH, MeOH, and $H_2O$. Among of these extracts, the EtOAc fraction which partitioned to 3.94% of the highest yields was to be the most active against all human pathogenic bacteria in this experiment. In addition, the antibacterial activities of the EtOAc fraction were more effective against Gram (+) bacteria compared to those against Gram (-) bacteria, which showed difference of the antibacterial activities against Gram (-) bacteria. To confirm the identity of the active substances, the EtOAc fraction was further separated by silica gel adsorption column, high performance liquid chromatography, and 98.48% purity of brazilin (1.67 mg)/EtOAc (10 mg) fraction was obtained from 300 g of C. sappan L. heart wood. The isolated active substance was a single compound of yellow crystalline, and was identified as brazilin ($C_{16}H_{14}O_5$) by MS, and $^lH$-NMR and $^{13}C$-NMR. These results suggest that the brazilin in the EtOAc fraction from MeOH extract of C. sappan L. has a potential as a natural therapeutic agent against human pathogenic Gram (+) bacteria such as Staphylococcus aureus.

• Journal of the Korean Applied Science and Technology
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• v.6 no.2
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• pp.11-19
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• 1989

In order to fractionate sardine oil by different solvents for an effective use of fish oil being subjected to the limit of use, an attempt was to investigate the proper solvents, ratios and fractionation time. The results of the study were as follows: 1. The proper solvent of fractionation using ethanol, isopropyl alcohol, acetone, and hexane was ethanol, and its optimum ratio was 2:1 (ethanol: oil, v/w). The proper time of ethanol fractionation by the ratio (2:1) was 4hr at $10^{\circ}C$, 6hr at $5^{\circ}C$, 8hr at $0^{\circ}C$and 8hr at $-5^{\circ}C$, respectively. 2. In the fractionation by stages using the ratio (2:1) at each temperature, the yield of stearine was 8％ at $10^{\circ}C$ (Fraction I), 32％ at $5^{\circ}C$ (Fraction II), 7％ at $0^{\circ}C$ (Fraction III) and 10％ at $0^{\circ}C$ (Fraction IV), respectively. When ethanol fractionation was undertaken at $5^{\circ}C$ by stages, the yield of stearine (Fraction II) was high. 3. Iodine value of Fraction II was 96.8. This result indicated that the hydrogenation process would be simplified by fractionation. 4. The percentage of the decrease of polyenoic acids from original sardine oil to Fraction II oil was from 30.5％ to 13.5％. The major fatty acids of Fraction II were palmitic and oleic acids and these fatty acids were about 52％ of total fatty acids. Therefore, Fraction II, which remained liquid oil at room temperature because solid fat content was 6.9％ at $20^{\circ}C$, would be used as frying oil.