• Korean Journal of Pharmacognosy
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• v.41 no.3
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• pp.161-165
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• 2010

A study was performed to assess the quality equivalence of commercially available Hwang-Ryun-Hae-Dok-Tang granules from various vendors in terms of the contents of characterizing compounds. 'Hwang-Ryun-Hae-Dok-Tang'(or 'Huanglian Jiedu Tang') is the complex medicinal herbal product which is composed of four different herbal drugs; Scutellariae Radix, Coptidis Rhizoma, Phellodendri Cortex and Gardeniae Fructus. Using a high performance liquid chromatography and direct analysis in real time mass spectrometer (DART-MS), five characterizing compounds such as baicalin, baicalein, berberine, palmatine, and geniposide were analyzed to compare the variance of the contents between the products from four different vendors. Although five characterizing compounds were all successfully detected from all the products, the contents of each compound were shown to have significant difference among vendors. For real time monitoring of characterizing compounds in medicinal herbal products, DART-MS can be successfully adopted as a powerful analytical tool.

• Journal of the Korean Wood Science and Technology
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• v.51 no.2
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• pp.145-156
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• 2023

Many skin diseases are caused by microbial infections. Representative pathogenic fungus and bacterium that cause skin diseases are Candida albicans and Staphylococcus aureus, respectively. Malassezia pachydermatis is a fungus that causes animal skin diseases. In this study, we propose a method for removing pathogenic microorganisms from the skin using relatively safe edible herbal extracts. Herbal extracts were screened for skin health through the removal of pathogenic microorganisms, and combinations for effective utilization of the screened extracts were identified. In this study, among methanol extracts of 240 edible plants, C. albicans, S. aureus, and M. pachydermatis were killed by extracts of 10 plants: Acori Gramineri Rhizoma, Angelicae Tenuissimae Radix, Cinnamomi Cortex, Cinnamomi Ramulus, Impatientis Semen, Magnoliae Cortex, Moutan Cortex Radicis, Phellodendri Cortex, Scutellariae Radix, and Syzygii Flos. By evaluating the synergistic antifungal activities against C. albicans using all 45 possible combinations of these 10 extracts, five new synergistic antifungal combinations, Acori Gramineri Rhizoma with Magnoliae Cortex extracts, Acori Gramineri Rhizoma with Phellodendri Cortex extracts, Angelicae Tenuissimae Radix with Magnoliae Cortex extracts, Magnoliae Cortex with Phellodendri Cortex extracts, and Phellodendri Cortex with Syzygii Flos extracts, were identified. By utilizing the selected extracts and five combinations with synergistic antifungal effects, this work provides materials and methods to develop new and safe methods for treating candidiasis using natural products.

• The Journal of Korean Medicine
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• v.42 no.3
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• pp.44-55
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• 2021

Objectives: As the use of herbal medicinal products (HMPs) increases worldwide, systematic verification of the safety of HMPs is required. The induction of cardiotoxicity is one of the major factors in post-approval withdrawal of medicinal products, and drug-induced cardiotoxicity assessment is emerging as an important step in drug development. In the present study, we evaluated human ether-à-go-go-related gene (hERG) potassium channel-related cardiotoxicity to predict the risk of cardiac arrhythmia in thirteen herbal medicines known to have cardiac toxicity. Methods: We measured the inhibition rate of hERG potassium channel activity of 13 medicinal herbal extracts in hERG-expressing HEK 293 cells using an automated patch-clamping system. Quinidine was used as a positive control for inhibition of hERG activity. Results: Extracts of Evodiae Fructus, Strychni Semen, and Corydalis Tuber potently inhibited the activity of hERG, and IC₅₀ values were 3.158, 19.87, and 41.26 ㎍/mL, respectively. Cnidi Fructus, Ephedra Herba, Lithospermi Radix, Polygoni Multiflori Radix, Visci Ramulus et Folium, Asiasari Radix et Rhizoma, and Scolopendra weakly inhibited hERG activity, and the IC₅₀ value for each herbal medicine was more than 400 ㎍/mL. Aconiti Kusnezoffii Tuber and two types of Aconiti Lateralis Radix Preparata (Po and Yeom) had weak inhibitory activity against hERG, and the IC₅₀ values were more than 700 ㎍/mL. The IC₅₀ value of quinidine against hERG was 1.021 𝜇M. Conclusion: Evodiae Fructus, Strychni Semen, and Corydalis Tuber acted as potent inhibitors against hERG. These herbal medicines may cause cardiac arrhythmia through QT prolongation, so care should be taken when taking them.

• Herbal Formula Science
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• v.31 no.2
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• pp.81-98
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• 2023

Objectives : This study aimed to analyze the trends in research and development projects related to herbal medicine and natural products in the field of traditional Korean medicine (TKM) over the past 20 years. Methods : Research projects were identified using "Korean medicine" as the subject heading in the National Science and Technology Information Service. The included projects investigated Korean medicine, natural products, or were related to the TKM industry. Data pre-processing and network analysis were performed using Python and Networkx package, and the network was visualized using the ForceAtlas2 visualization algorithm. Results : 1. Over the study period, 4,020 projects were conducted with a research budget of KRW 835.2 billion. Seven institutions performed over 100 projects each, accounting for 2.4% of all participating institutions, and the top 10 institutions accounted for 58.9% of total projects. 2. Obesity was the most frequently mentioned disease-related keyword. Chronic or age-related diseases such as diabetes, osteoporosis, dementia, parkinson's disease, cancer, inflammation, and asthma were also frequent research topics. Clinical research, safety, and standardization were also frequently mentioned. 3. Centrality analysis found that obesity was the only disease-related keyword identified, alongside TKM-related keywords. Standardization, safety, and clinical trials were identified as central keywords. Conclusions : The study found that research projects in TKM have focused on standardizing and ensuring the safety of herbal medicine, as well as on chronic and age-related diseases. Clinical studies aimed at verifying the effectiveness of herbal medicine were also frequent. These findings can guide future research and development in herbal medicine.

• 한국약용작물학회:학술대회논문집
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• 2011.09a
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• pp.162-163
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• 2011

• Advances in Traditional Medicine
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• v.6 no.1
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• pp.1-11
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• 2006

Herbal medicines are widely used for centuries to treat illness and improve health in Asia and the use of these remedies has become a worldwide form of alternative therapy. However, there is very limited evidence from randomized controlled trials to support the efficacy of the vast majority of herbal products. Of the commonly used herbal medicines, systemic clinical experiences and promising experiments in animals have found potential evidence of efficacy for six herbal medicines (Unkei-to, Hachimijio-gan, Keishi-bukuryo-gan, Toki-shakuyaku-san, Shakuyaku-kanzo-to, Sairei-to) in the treatment for female reproductive dysfunction. Because herbs may contain potent bioactive substances, the physician should have an opportunity to outline more stringent regulation, similar to over-the counter drug.

• Journal of Haehwa Medicine
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• v.17 no.1
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• pp.53-57
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• 2008

These day, herbal drugs have been widely used in all over the world, as primary therapeutics or supplements for treating various diseases. Herbal drugs are generally regarded as non-toxic due to their natural origin and long history traditionally used without serious adverse reactions. However, plenty warnings have been reported, particularly about the potential hepatotoxicity of herbal products. This report would be helpful for understanding theory of toxicology and prevent from herbal drug-derived hepatotoxicity in Oriental medicinal field.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.368.1-368.1
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• 2002

Mesaconitine and hypaconitine were isolated from Cho O and identified by the spectroscopic methods. The contents of alkaloid (mesaconitine. aconitine and hypaconitine) in the Cho O and its processed products were determined by high performance liquid chromatography. Processed 1 and 2 methods reduced the contents of alkaloid than those of processed 3 and commercially processed Aconiti Tuber powder. (omitted)

• Journal of Sasang Constitutional Medicine
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• v.23 no.1
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• pp.8-11
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• 2011

1. Objectives: Sixty eight kinds of single herbal medicine extracts and fifty six kinds of mixed herbal medicine extracts have been used under a national health insurance since 1987. The number of herbal medicine extracts under an insurance coverage remains unchanged. The demand for covering complex herbal medicine extracts and Sasang Constitutional medicine extracts in a national health insurance increases. This study is to investigate the needs for the insurance coverage. 2. Methods: Advantages and disadvantages between herbal medicine decoction and extracts were explained and Sasang Constitutional medicine extracts according to Sasang constitution were surveyed in production nowadays. 3. Results: Herbal medicine decoction has many advantages of treating patients but herbal medicine extracts have advantages of the rapid adminstration and being easy to carry. From eleven to twenty two products of Sasang Constitutional medicine extracts have been in production in several factories. There are eleven kinds of Soyangin medicine extracts, nine kinds of Taeeumin medicine extracts and five kinds of Soeumin medicine extracts. 4. Conclusions: Complex herbal medicine extracts and Sasang Constitutional medicine extracts have to be in use under a national health insurance as soon as possible.

• Korean Journal of Pharmacognosy
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• v.43 no.3
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• pp.250-256
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• 2012

In order to extract the curcuminoid such as curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) in turmeric (Curcuma longa), solvent extraction methods (dipping and ultrasonic extraction method) and solid-phase extraction (SPE) were used. RP-HPLC (reverse-phase high-performance liquid chromatography) and TLC (thin-layer chromatography) were used for identification and analysis the three curcuminoid. From the experimental results, it is evident that the percentage of curcuminoid extracted from turmeric by ultrasonic extraction method was higher than dipping method. The percentage of curcumin extracted from turmeric by pure methanol was higher than any aqueous methanolic composition. Moreover, the total peak area of three curcuminoid was above 92% in RP-HPLC using solid-phase extraction. These results will form a database for investigating the constituents of natural products and the resources of pharmaceutical, nutrition, and cosmetic products.