• 대한한방소아과학회지
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• 제21권1호
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• pp.189-209
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• 2007

Objectives : In order to study the effect of Dangkwiyughwangtang and Okbyoungpoongsangamibang on the immune response induced by methotrexate in mice. Method : Delayed type of hypersensitivity, hemagglutinin titer, hemolysin titer, rosette forming cells, phagocytic activity for immune response, lymphocyte transformation, and productivity of Interleukin-2 were measured. Results : Body weight decreasing was significantly inhibited as compared with control group in both the Dangkwiyughwangtang and Okbyoungpoongsangamibang groups. Delayed type of hypersensitivity was significantly increased as compared with control group in both groups Hemagglutinin titer was significantly increased as compared with control group in both groups. Hemolysin titer was significantly increased as compared with control group in the Okbyoungpoongsangamibang group. Rosette forming cells were significantly increased as compared with control group in both groups. Phagocytic activity for immune response was slightly decreased in the Dangkwiyughwangtang group and slightly increased in the Okbyoungpoongsangamibang group insignificantly as compared with the control group. Lymphocyte transformation was significantly increased as compared with the control group in both the Dangkwiyughwangtang and Okbyoungpoongsangamibang groups. Productivity of Interleukin-2 was significantly increased as compared with the control group in both the Dangkwiyughwangtang and Okbyoungpoongsangamibang groups. Conclusion : Both the Dangkwiyughwangtang and Okbyoungpoongsangamibang groups enhance immunity in mice.

• 대한한방종양학회지
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• 제6권1호
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• pp.47-65
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• 2000

In order to investigate antitumor and immune response effect by Hyangsapyungwisan after Sarcoma-180 cells and methotrexate were treatred each other, the extract of Hyangsapyungwisan was orally administered to ICR mice for 14 days. To evaluate the effects of the Hyangsapyungwisan, 50% inhibition concentration($IC_{50}$), mean survival days, tumor weight for antitumor effects, hemagglutinin titer, hemolysin titer, rosette forming cells, natural killer cell activity and productivity of interleukin-2 for immune responses measured in ICR mice. The results were summarized as follows: 1. Mean survival time in Hyangsapyungwisan-treated group was slightly prolonged, as compared with control group(13.46%). 2. On the MTT assay, cell viability was significantly inhibited by $5{\mu}g/well,\;2.5{\mu}g/well,\;1.25{\mu}g/well,\;and\;0.625{\mu}g/well$ of Hyangsapyung-wisan concentration inhibited cell viability significantly. $IC_{50}$ for cell viability was $11.59{\mu}g/well$. 3. Tumor weight in Hyangsapyungwisan treated group was depressed, as compared with the control group(p<0.05). 4. Hemagglutinin titer in Hyangsapyungwisan-treated group was slightly increased with no significance, as compared with the control group. 5. Hemolysin titer in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(p<0.05). 6. Rosette forming cells in Hyangsapyungwisan-treated group was silightly increased, as compared with the control group(p<0.05). 7. Naural killer cell activity in Hyangsapyungwisan-treated group was significantly increased(p<0.05). 8. Production of interleukin-2 was significantly increased(p<0.05). According to the above results, Hyangsapygwisan had prominent antitumor effects, and enhance both cellular and humoral immunity in mice.

• 대한한방내과학회지
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• 제24권2호
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• pp.315-328
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• 2003

This study was performed to investigate the effect of Bunsimgieum on antitumor effect after sarcoma-180 cells transplantation into peritoneal cavity or left groin and immune responses on the depressed immunity induced by methotrexate in mice. The Bunsimgieum extract of 10mg/kg was orally administered 14 days for antitumor effects and 21 days for immune responses. 50% inhibitory concentration($IC_{50}$) of SUN-1, SUN-C4, and SUN-396 cancer cell, mean sunvival days and body weight of tumor bearing mice, and growth of tumor mass for antitumor effect; delayed type hypersentivity, hemagglutinin titer, hemolysis titer, rosette forming cells, natured killer cell activity, lymphocyte transformation, productivity of interleukin-2, and phagocytic activity for their immune responses were measured in ICR mice. Significance in antitumor effect is noted in the enlongation of mean life days and inhibition of tumor growth(p<0.01, respectively). Significance of immune responses is also noted in hemolysis titer, lymphocyte transfumotion, IL-2 productivity, phagocytic activity, and natural killer cell activity at E/T ratio 100:1(p<0.01, respectively). Significant in rosette cell formation was seen at dosage of 20mg/kg(p<0.01). However, Difference of body weight as antitumor effect, delayed type hypersensitivity, and hemagglutinin titer were not shown significantly. According to the above results, it could be suggested that Bunsimgieum has prominent antitumor and immunity enhancing effect.

• Environmental Analysis Health and Toxicology
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• 제23권4호
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• pp.301-306
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• 2008

To investigate the effects of carboxyethylgermanium sesquioxide (Ge-132) on the cyclophosphamide (CY)-induced immunotoxicity, hemagglutinin titer (HA-titer), splenic IgM plaque forming cells (PFC) and contact-delayed type hypersensitivity (CDTH) were assessed in mice. Ge-132 was orally administered alone (single dose of 300, 600, 900 mg/kg b.w.) or with CY (10 or 50 mg/kg, i.p.) to mice on the 2nd day before, simultaneously with, the 2nd day after immunization. Within Ge-132 alone-treated groups, HA-titer and PFC to SRBC were significantly and dose-dependently enhanced when compared with control group. HA-titer and PFC numbers suppressed by the treatment of CY alone were significantly restored by the concomitant treatment of CY and Ge-132. Also, Ge-132 significantly decreased DNFB-induced CDTH and inhibited the CY-enhanced CDTH. These results indicate that Ge-132 may be able to increase humoral immunity and inhibit the immunotoxicity by CY.

• 한국식품영양과학회지
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• 제30권6호
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• pp.1283-1286
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• 2001

마크로파지의 활성을 조사하기 위해 carbon clearance time을 조사한 결과 AEY 처리구가 control이나 CEY 처리구에 비해 짧았다. 특히 AEY 500$\mu\textrm{g}$ 처리에서 carbon clearance 시간이 5분으로 control에서 9.42분, CEY(250$\mu\textrm{g}$ 처리)에서 9.01분보다 유의성 있는 감소를 보였다. AEY 처리에 의해 응집소가와 용혈소가가 다소증가되었다. SRBC처리 1일째와 3일째에 250$\mu\textrm{g}$ 처리구를 비교해 보면, control, CEY, AEY의 응집소가는 각각 5.50, 5.63, 6.00 및 5.25, 5.38, 5.50로 용혈소가는 각각 4.75, 5.38, 5.50 및 4.25, 5.63, 5.63으로 AEY 처리구가 control, CEY 처리구에 비해 면역 활성이 있었지만 유의성은 없었다.

• Pediatric Infection and Vaccine
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• 제6권1호
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• pp.93-100
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• 1999

목 적 : Mycooplasma 폐렴은 소아과 영역에서 통상 사용하지 않는 macrolide 계열의 항생 제에 감수성이 있어서 초기에 신속한 진단이 중요한데, 이에 저자들은 발병 1주일 이내의 Mycoplasma 폐렴 환아에서 Mycoplasma 항체역가, 냉혈응집소역가, ESR, CRP 등과 임상 소견을 검토하여 진단에 어떤 의의가 있는지 알아보고자 하였다. 방 법 : 1997년 1월부터 12월까지 원광의대병원 소아과에 입원하여 Mycoplasma 폐렴으로 진단되어 치료를 받았던 환아 중에 입원 전에 발열과 기침이 7일 이내였던 환아 56명을 대상으로 M. pneumoniae 균의 항체검사를 시행하였다. 대상 환아를 항체역가가 1:320 미만이었던 어린이 33명(A군)과 1:640 이상이었던 어린이 23명(B군)으로 나누어 입원시에 총 백혈구수와 분획, 혈구 침강속도 및 CRP와, 냉혈응집소치, 흉부 X-선 소견을 비교 검토하였다. Mycoplasma 폐렴의 진단 기준은 기침과 발열 등의 임상증상과 흉부 청진상수포음의 존재, 흉부 X-선 소견상 폐 실질에 침윤을 보이며 indirect particle agglutination test로 측정한 Mycoplasma 항체가가 1:640 이상이거나, 1주일 후에 재검사에서 4배 이상 역가가 증가한 경우로 하였으며 냉혈응집소는 역가가 1:64 이상인 경우를 양성 판정하였다. 결 과 : 1) 남녀 비는 1:1.4이었고 평균연령은 $5.8{\pm}2.96$세 이었다. 2) 내원시 평균 체온은 $38.5{\pm}0.1^{\circ}C$이었으며 52례(93%)에서 객담성 기침이 관찰되었다. 3) 총백혈구수는 Mycoplasma 항체 역가가 640이상으로 높았던 어린이군에서 $10,470{\pm}877.0/mm^3$로 320 이하이었던 어린이군 $7,761{\pm}508.5/mm^3$에 비해 의의 있게 높았다(P<0.014). 4) 혈구침강속도와 CRP는 각각 $27.2{\pm}26.82/mm/hr$, $39.9{\pm}41.50ng/mL$로 의미 있는 상승을 보이지 않았다. 5) 입원시 흉부 X-선 소견상 폐 침윤은 우측폐에 24례(43%)로 가장 흔하게 볼 수 있었고 이중에서도 우측 하엽에서 10례로 가장 흔하였다. 6) 냉혈응집소 역가는 Mycoplasma 항체 역가와 의의 있는 상관 관계를 보이지 않았고 25례(45%)에서 냉혈응집소 역가가 1:64 미만이었다. 결 론 : 발병초기 Mycoplasma 폐렴의 진단은 임상증상, 흉부 X-선 소견 및 항체검사가 유용하며 초기 항체 역가는 임상소견의 정도와는 무관하므로 항체검사가 필수적이며 냉혈응 집소검사는 양성률이 낮아서 발병초기에 Mycoplasma 폐렴의 진단에 도움을 주지 못할 것으로 사료된다.

• 혜화의학회지
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• 제9권2호
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• pp.123-134
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• 2001

In order to investigate the effect of Mori Folium on immunologic control function, the various immune responses were studied. The results were obtained as follows : 1. The transformation of lymphocyte was increased significantly in all concentration of Mori Folium treated group as compared with negative and positive control. 2. In FACS analysis, the population of helper T cell and T cell were increased significantly in Mori Folium treated group as compared with control. 3. IL-12(p35) and INF-${\gamma}$ were upregulated in Mori Folium treated group 4. NO and carbon clearance were significantly increased in Mori Folium treated group as compared with control. 5. The hemagglutinin titer and hemolysin titer were increased but insignificantly in Mori Folium treated group as compared with control.

• 대한한방종양학회지
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• 제3권1호
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• pp.169-191
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• 1997

Hyangpaeyangyeongtang, which was first prescribed in the book of Euijonggumgam (醫宗金鑑), has been widely applied to the treatment of scrofula and carbuncle in the neck and tumor as well in the oriental medicine. The Fritillariae roylei bulbus was also used as an anti-tumor medicine. In order to investigate the effect of Hyangpaeyangyeongtang and Fritillariae roylei bulbus extracts on the immune responses to the epithelioma induced by 3-methylcholanthrene and prostaglandin-$F_2{\alpha}$ in mice, the change of epithelioma size was observed and delayed type hypersensitivity, hemagglutinin titer, hemolysin titer, rosette forming cells, natural killer cell activity, lymphocyte transformation, productivity of interleukin-2 and phagocytic activity were also measured. The results were as follows; 1. The epithelioma size was decreased with statistical significance in the Hyangpaeyangyeongtang group at the 14th day, as compared with the control group. 2. Delayed type hypersensitivity was not shown any decrease in the Hyangpaeyangyeongtang group and the Fritillariae roylei bulbus group, as compared with the control group. 3. Hemagglutinin titer was increased with statistical significance in the Hyangpaeyangyeongtang group, but not in the Fritillariae roylei bulbus group as compared with the control group. 4. Hemolysin titer was increased with statistical significance in the Hyangpaeyangyeongtang group, but not in the Fritillariae roylei bulbus group as compared with the control group. 5. Rosette forming cells were not increased in the Hyangpaeyangyeong tang group, but increased with statistical significance in the Fritillariae roylei bulbus group as compared with the control group. 6. Natural killer cell activity at the ratio 100:1 of effector cells to target cells was increased with statistical significance in the Hyangpaeyayeongngtang group and the Fritillariae roylei bulbus group respectively, and the natural killer cell activity at the ratio of 50:1 and 10:1 of effector cells to target cells Hyangpaeyangyeongtang group was increased with statistical significance as compared with the control group. 7. Lymphocyte transformation was increased in the Hyangpaeyangyeong tang group and the Fritillariae roylei bulbus group respectively, as compared with the control group. 8. Productivity of interleukin-2 was increased in the Hyangpaeyangyecing tang group and the Fritillariae roylei bulbus group respectively, as compared with the control group. 9. Phagocytic activity was not shown any decrease in the Hyangpaeyangyeongtang group and the Fritillariae roylei bulbus group, as compared with the control group.

• 대한한방종양학회지
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• 제3권1호
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• pp.1-27
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• 1997

This study was performed to investigate the effects of Eunwhasagantang and Eunwhasagantangganokyong on the viability of tumor cells in vitro(MTT assay), on antitumor effects after Sarcoma-180 cells transplantation into the peritoneal cavity or left groin, and on decreased immune responses in mice induced by methotrexate. The extracts of its herbal medicines were orally administered for 14 or 21 days. To evaluate the effects of the Eunwhasagantang and Eunwhasagantangganokyong many items such as 50% inhibitory concentration($IC_{50}$), mean survival days, tumor and body weight for antitumor effects, and delayed type hypersensitivity, hemagglutinin titer, hemolysin titer, rosette forming cells, natural killer cell activity, lymphocyte transformation, productivity of interleukin-2 and phagocytic activity for immune responses were measured in ICR mice. The results were obtained as follows; 1. $IC_{50}$ of Eunwhasagantang treated group was 0.000204mg/ml on SNU-396 and that of Eunwhasagantangganokyong treated group was 0.000103mg／ml on SNU-1, those results indicate that the medicine has high antitumor activity. 2. Mean survival times in Eunwhasagantang and Eunwhasagantangganokyong treated groups were slightly increased with no significance, as compared with the control group. 3. Tumor weight in Eunwhasagantang and Eunwhasagantangganokyong treated group was depressed, as compared with the control group(p<0.01). 4. Body weight in Eunwhasagantang and Eunwhasagantangganokyong treated group was significantly increased, as compared with the control group(p<0.05). 5. Delayed type hypersensitivity in Eunwhasagantang and Eunwhasagantang-ganokyong treated group was slightly decreased with no significance, as compared with the control group. 6. Hemagglutinin titer in Eunwhasagantang and Eunwhasagantangganokyong treated group was slightly increased with no significance, as compared with the control group. 7. Hemolysin titer only in Eunwhasagantang treated group was significantly increased, as compared with the control group(p<0.01). 8. Rosette forming cells only in Eunwhasagantangganokyong treated group was slightly increased with no significance, as compared with the control group. 9. In the NK cell activity, the ratio of effector cells and target cells of the Eunwhasagantang treated group was significantly increased(p<0.01) in case which the ratio was 100: 1, and that of the Eunwhasagantangganokyong treated group was significantly increased(P<.01, p<0.05) in case which the ratio was 100:1, 50:1, as compared with the control group. 10. Lymphocyte trasnformation in Eunwhasagantang and Eunwhasagantangganokyong treated group was significantly increased, as compared with the control group(p<0.01). 11. Interleukin-2 in Eunwhasagantang and Eunwhasagantangganokyong treated group was significantly increased, as compared with the control group(p<0.05, p<0.01). 12. Phagocytic activity in Eunwhasagantang and Eunwhasagantangganokyong treated group was significantly increased, as compared with the control group(p<0.05). According to the above results, it could be suggested that Eunwhasagantang and Eunwhasagantangganokyong have prominent antitumor effects, and enhance both cellular and humoral immunity.

• 대한한방종양학회지
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• 제1권1호
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• pp.141-165
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• 1995

In order to investigate the effects of Banhabaekchulcheonmatang and Banhabaekchulcheonmatanggamibang on antitumor effects after Sarcoma-180 cells transplantation into the peritoneal cavity or left groin in mice, and immune responses in mice induced by methotrexate, the extracts of its herbal medicines were orally administered for 14 or 21 days. Experimental studies were performed for measurance of mean survival days, tumor and body weight for antitumor effects, and delayed type hypersensitivity, hemagglutinin titer, hemolysin titer, rosette forming cells, natural killer cell activity and phagocytic activity for immune responses in ICR mice. Following results were obtained : 1. Mean survival time in Banhabaekchulcheonmatang and Banhabaekchulcheonmatanggamibang-treated group was significantly prolonged, as compared with the control group(P<0.010, P<0.005). 2. Tumor weight in Banhabaekchulcheonmatang and Banhabaekchulcheonmatanggamibang-treated group was significantly depressed, as compared with the control group(P<0.050, P<0.050). 3. Body weight in Banhabaekchulcheonmatang-treated group was significantly increased (P<0.050), but in Banhabackchulcheonmatanggamibang-treated group was slightly increased with no effectivenes, as compared with the control group. 4. Delayed type hypersensitivity in Banhabaekchulcheonmatang and Banhabaekchulcheonmatanggamibang-treated group was significantly increased, as compared with the control group(P<0.010, P<0.050). 5. Hemagglutinin titer in Banhabaekchulcheonmatang and Banhabaekchulcheonmatanggamibang-treated group was significantly increased, as compared with the control group(P<0.050, P<0.050). 6. Hemolysin titer in Banhabaekchulcheonmatanggamibang-treated group was significantly increased (P<0.050), but in Banhabaekchulcheonmatanggamibang-treated group was slightly increased with no effectiveness, as compared with the control group. 7. Rosette forming cells in Banhabaekchulcheonmatang and Banhabaekchulcheonmatanggamibang-treated group was slightly increased with no effectiveness, as compared with the control group. 8. Natural Killer cell activity in Banhabaekchulcheonmatang and Banhabaekchulcheonmatanggamibang-treated group was slightly increased with no effectiveness, as compared with the control group. 9. Phagocytic activity in Banhabaekchulcheonmatanggamibang-treated group group was significantly increased (P<0.050), but in Banhabaekchulcheonmatanggamibang-treated group was increased with no effectiveness, as compared with the control group. According to the above results, it could be suggested that Banhabaekchulcheonmatang and Banhabaekchulcheonmatanggamibang have prominent antitumor effects, and enhance both cellular and humoral immunity.