Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of the Egg Yolks from Laying Hens Intubated Astaxanthin on the Macrophage Activity, Hemagglutinin-titer and Hemolysin-titer

Astaxanthin처리 산란계로부터 생산된 난황이 Mouse의 마크로파지 활성과 응집소가 및 용혈소가에 미치는 영향

  • 김홍출 (진주산업대학교 미생물공학과) ;
  • 박숙자 (경상대학교 응용화학식품공학부) ;
  • 김정곤 (경상대학교 응용화학식품공학부) ;
  • 김영림 (경상대학교 응용화학식품공학부) ;
  • 박원석 (경상대학교 응용화학식품공학부) ;
  • 조용운 (진주산업대학교 미생물공학과) ;
  • 조현종 (농협중앙회 식품연구소) ;
  • 김정환 (경상대학교 응용화학식품공학부) ;
  • 하영래 (경상대학교 응용화학식품공학부)
  • Published : 2001.12.01
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Abstract

Effect of the egg yolks from laying hens intubated, p.o., astaxanthin (designated AEY) on mouse humoral immunity was investigated using male ICR mouse (6~7 weeks of age). Mice were adapted in a temperature- and humidity- controlled house for one week and randomly divided into 5 treatment groups (9 mice/cage/treatment). Mice were intubated p.o., AEY (100, 250 and 500$\mu\textrm{g}$) or control egg yolks (CEY, 250$\mu\textrm{g}$), dissolved in 0.1 mL DMSO, for consecutive 4 days. At day 5, carbon suspension (pilot drawing ink 3 mL+3% gelatine 3 mL) was injected 3 $\mu$L Per 1 g body weight through tail vein. Carbon clearance time was measured at 5 and 35 minutes Post the injection of carbon suspension. Another two experiments were conducted to determine the hemagglultinin-titer (HGT) and hemolysin-titer (HLT) with male ICR mouse (8 mice/cage/treatment). Mice treated with AEY were induced immune activity with SRBC. HGT and HLT were measured from the blood at day 1 and 3 after treatment of SRBC. AEY treatment reduced the carbon clearance time. Especially the carbon clearance time by 500 $\mu\textrm{g}$ AEY treatment was 5.00 minutes, which was very short time compared with 9.42 minutes by control and 9.01 minutes by CEY. AEY group showed slights higher values of HGT and HLT than CEY group and control. At day 1, HGT in control, 250$\mu\textrm{g}$ CEY and 250$\mu\textrm{g}$ AEY groups was 5.50, 5.63, and 6.00, respectively. Similiarly, HLT in control, 250$\mu\textrm{g}$ CEY and 250$\mu\textrm{g}$ AEY groups was 4.75, 5.38, and 5.50, respectively, at day 1. These results suggest that AEY exhibited immunity-enhancing effect.

마크로파지의 활성을 조사하기 위해 carbon clearance time을 조사한 결과 AEY 처리구가 control이나 CEY 처리구에 비해 짧았다. 특히 AEY 500$\mu\textrm{g}$ 처리에서 carbon clearance 시간이 5분으로 control에서 9.42분, CEY(250$\mu\textrm{g}$ 처리)에서 9.01분보다 유의성 있는 감소를 보였다. AEY 처리에 의해 응집소가와 용혈소가가 다소증가되었다. SRBC처리 1일째와 3일째에 250$\mu\textrm{g}$ 처리구를 비교해 보면, control, CEY, AEY의 응집소가는 각각 5.50, 5.63, 6.00 및 5.25, 5.38, 5.50로 용혈소가는 각각 4.75, 5.38, 5.50 및 4.25, 5.63, 5.63으로 AEY 처리구가 control, CEY 처리구에 비해 면역 활성이 있었지만 유의성은 없었다.

Keywords

References

  1. Helv. Chim. Acta. v.64 Natural occurrence of enatiomeric and meso-astaxanthin 5. Ex wild salmon Schiedt. K.;Leuenberger. F.J.;Vecchi. M.
  2. Acta. Hydrobiol. v.17 Carotenoids in fish Kralow. P.O.
  3. Tohoku J. Agric Res. v.24 Studies on astaxanthin in some fresh-water fishes Hata. M.
  4. Nippon suisan Gakkaishi. v.50 Behavior of carotenoids in the chum salmon on corhynchus keta during development Kitahara. T.
  5. Ph.D. thesis Metabolism of dietary carotenoids an bicconversion pathways of retinoids in Masu salmon, Oncorhynchus macrostomus an Eel, Anguilla japonica Park. M.Y.
  6. Aquaculture v.95 Flesh pigmentation of rainbow trout fed astaxanthin or canthaxanthin at different feeding rates in fresh water and saltwater Storebakken. T.;Choubert. G.
  7. Pure Appl. Chem. v.57 Absorption, retention, and metabolic transformation of carotenoids in rainbow trout, salmon, and chicken Schiedt. K.;Leuenberger. F.J.;Vecchi. M.;Grinz. M.
  8. Carassius auratus. Biochem. Soc. Trans. v.14 Retinol and 3m,4 - dehydroretinol formation from xanthophylls in the gold fish Davies . B.W.;Davies. B.H.
  9. Korean J. Toxicol. v.8 Immunopathology of spleen following 7,12-dimethylbenz[α]anthracene treatment in BALB/c mice Lee. D.Y.;Han. S.S.;Lee. S.M.
  10. J. Mar. Biotechnol. v.2 Carotenoids as free radical scavengers in marine animals Miki. W.;Otaki. N.;Shimidzu. N.;Yokoyama. A.
  11. Fisheries Sci. v.62 Carotenoids as singlet oxygen quenchers in marine organisms Nobuyoshi. S.;Masafumi. G.;Wataru. M.
  12. Carcinogenesis v.19 Dietary carotenoids inhibit aflatoxin B₁-induced liver preneoplastic foci and DNA damage in the rat: Role of the modulation of aflatoxin B₁ metabolism Gradelet. S.;Le. Bon. A.M.;Berges. R.;Suschetet. M.;Astorg. P.
  13. Carcinogenesis v.16 Suppression of azoxymethaneinduced rat colon carcinogenesis by dietary adminstration of naturally occurring xanthophylls astaxanthin and canthaxanthin during the postinitiation phase Tanaka. T.;Kawamori. T.;Ohnishi. M.;Makita. H.;Mori. H.;Satoh. K.;Hara. A.
  14. Cancer Res. Baltimore v.55 Chemorevention of rat oral carcinogenesis by naturally occurring xanthophylls, astaxanthin and canthaxanthin Tanaka. T.;Makati. H.;Ohnishi. M.;Mori. H.;Satoh. K.;Hara. A.
  15. Appl. Microbiol. Biotechnol. v.48 Antioxidant role of astaxanthin in the green alga Haematococcus pluvialis Kobayashi. M.;Kakizono. T.;Nishio. N.;Nagai. S.;Kurimura. Y.;Tsuji. Y.
  16. J. Agric. Food Chem. v.38 Effects, quenching mechanisms, and kinetics of carotenoids in chlorophyll-sensitized photooxidation of soybean oil Lee. S.H.;Min. D.B.
  17. J. Ind. Micro. v.14 Carotenoids protect phaffia rhodozyme against singlet oxigen damage Schroeder. W.A.;Johmson. E.A.
  18. J. Ferment. Bioengin. v.75 Improvement of astaxanthin production by Phaffia rhodozyma through mutation and optimization of culture conditions Fang. T.J.;Cheng. Y.S.
  19. Environmental Mutagens & Carcinogens v.18 Inhibition of DMBA-induced mouse epidermal carcinogenesis by astaxanthin-containing egg yolks Lee. S.H.;Park. C.W.;Lee. Y.C.;Choi. E.S.;Kim. M.N.;Ha. Y.L.
  20. J. Korean Soc. Agric. Chem. Biotechnol. v.40 Inhibition of benzo[a]pyrene-induced mouse forestomach neoplasia by astaxanthin-containing egg yolks Lee. S.H.;Park. C.W.;Park. W.S.;Lee. Y.C.;Choi. E.S.;Ha. Y.L.
  21. J. Korean Soc. Food Sci. Nutr. v.27 Inhibition of sarcoma-180 cell-induced mouse ascites cancer by astaxanthin-containing egg yolks Lee. S.H.;Park. C.W.;Park. K.A.;Lee. Y.C.;Choi. E.S.;Ha. Y.L.
  22. J. Korean Soc. Food Sci. Nutr. v.30 Overcome effect of catabolic response in mouse by the egg yolks from laying hens intubated astaxanthin Kim. H.C.;Park. S.J.;Park. C.W.;Kim. Y.R.;Park. K.A.;Choi. E.S.;Cho. Y.U.;Ha. Y.L.