• Microbiology and Biotechnology Letters
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• v.13 no.2
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• pp.137-144
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• 1985

Studies were conducted on the conditions for preparation of yeast protoplasts utilizing Hansenula anomala var. anomala FRI YO-32 as well as Saccharomyces cerevisiae KFCC 32356 and a lytic enzyme from the snail Helix pomatia. The cell wails of the strain FRI YO-32 and S cerevisiae were found to be resistant to activity of the snail lytic enzyme if they were not treated with thiol compounds. Dithiothreitol was found to be more effective than 2-mercaptoethanol, but the latter was considered to be practical. As factors influencing the formation of yeast protoplast, it was considered to be concentration and incubation time of 2-mercaptoethanol or the lytic enzyme, growth stages in yeast cultivation, initial number of yeast cells, and concentration of osmotic stabilizer (KCI). Optimum conditions for the preparation of yeast protoplasts were determined.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.63-63
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• 2003

$\alpha$$_1$-Antityrpsin is a member of the serine protease inhibitor (SERPIN) family that shares a common tertiary structure. The reactive site loop (RSL) of serpins is exposed at one end of the molecule for protease binding. Upon cleavage by a target protease, the RSL is inserted into the major $\beta$-sheet A, which is a necessary process for formation of a tight inhibitory complex. Various biochemical and structural studies suggest that the rate of the RSL insertion upon binding a target protease is critical for inhibitory activity, and it is thought that helix F region (thFs3A and helix F) located in front of $\beta$-sheet A, should be lifted for the loop insertion during complex formation.

• Journal of the Korean Magnetic Resonance Society
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• v.8 no.2
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• pp.127-138
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• 2004

We obtained the chemical shifts of amide protons (NHs) in helical peptides at various temperatures and trifluoroethanol (TFE) concentrations using 2-dimensional NMR spectroscopy. These NH chemical shifts and their temperature dependence exhibited characteristic periodicity of 3-4 residues per cycle along the helix, where downfield shifted NHs showed larger temperature dependence. In an attempt to understand these observations, we focused on hydrogen bonding changes in the peptides and examined the validity of two possible explanations: (1) changes in intermolecular hydrogen bonding caused by differential solvation of backbone carbonyl groups by TFE, and (2) changes in intramolecular hydrogen bonding due to disproportionate variations in the hydrogen bonding within the peptide helix. Interestingly, the slowly exchanging NHs, which were on the hydrophobic side of the helix, showed consistently larger temperature dependences. This could not be explained by the differential solvation assumption, because the slowly exchanging NHs would become more labile if the preceding carbonyl groups were preferentially solvated by TFE. We suggest that the disproportionate changes in intramolecular hydrogen bonding better explain both the temperature dependence and the exchange behavior observed in this study.

• Parasites, Hosts and Diseases
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• v.53 no.5
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• pp.647-651
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• 2015

The aim of this study was to determine the presence and prevalence of larval stages of Dicrocoelium dendriticum and Brachylaima sp. in the first intermediate host, a species of land snail, Helix aspersa, in Turkey. A total of 211 snails were collected in April-May 2014 from pastures in Mersin District. Larval stages of D. dendriticum were identified under a light microscope. Hepatopancreas from naturally infected H. aspersa snails were examined histologically. The prevalence of larval stages of D. dendriticum and Brachylaima sp. in H. aspersa snails was found to be 2.4% and 1.9%, respectively, in Mersin, Turkey. Cercariae were not matured in sporocysts at the beginning of April; however, it was observed that cercariae matured and started to leave sporocysts by early-May. Thus, it was concluded that H. aspersa acts as an intermediate host to D. dendriticumin and Brachylaima sp. in Mersin, Turkey. A digenean trematode Brachylaima sp. was seen for the first time in Turkey.

• Journal of Engineering Education Research
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• v.21 no.6
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• pp.54-62
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• 2018

This study is an analysis of the process of the discovery of the DNA double helix structure from an engineering literacy education perspective. The explanation of the DNA double helix structure by James Watson and Francis Crick in 1952 is a well-known scientific episode. The process is also a combination of various incidents that can frequently happen in competitive engineering research and development situations. Therefore, the process of the discovery of the DNA structure is a remarkable event that can cover all subjects, such as engineering and ethics, research ethics, communication between researchers, engineering and leadership, engineering and teamwork, and engineering and women. This paper focuses on analyzing the research ethics issues associated with Rosalind Franklin and comparing and analyzing the three teams that were very close to the discovery of the DNA structure. By looking at why the Watson and Crick team got the final answer instead of the Linus Pauling's team or the Maurice Wilkins and Rosalind Franklin's team, the virtues of the technology development process that should be taught in engineering literacy education will be naturally presented.

• Journal of the Korean Society for Precision Engineering
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• v.14 no.12
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• pp.143-152
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• 1997

The deflection of an end mill is very important in machining process and cutting simulation because it affects directly workpiece accuracy, cutting force, and chattering. In this study, the deflection of the end mill was studied both experimentally and by using finite element analysis. And the moment of inertia of cross sections of the helical end mill is calculated for the determination of the relation between geometry of radial cross section and rigidity of the tools. Using the Bernoulli-Euler beam theory and the concept of equivalent diameter, a deflection model is established, which includes most influences from tool geomety parameters. It was found that helix angle attenuates the rigidity of the end mill by the finite element analysis. As a result, the equivalent diameter is determined by tooth number, inscribed diameter ratio, cross sectional geometry and helix angle. Because the relation betweem equivalent diameter and each factor is nonlinear, neural network is used to decide the equivalent diameter. Input patterns and desired outputs for the neural network are obtained by FEM analysis in several case of end milling operations.

• Journal of the Korean Geosynthetics Society
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• v.13 no.4
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• pp.77-85
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• 2014

The helical pile has become popular with some constructional advantages because relatively compact equipment is needed for installing helical piles. However, field loading tests for estimating the bearing capacity of helical piles have drawbacks that the required dead load should be as much as the operation load, and reaction piles or anchors are required. In this paper, the bi-directional load test without necessity of reaction piles and loading frames was applied to the helical pile, and the load-settlement curves of the helical piles were measured. The bi-directional load test was performed in two separate stages with the aid of a special hydraulic cylinder whose diameter is equal to that of the pile shaft. In the first stage, the hydraulic cylinder is assembled immediately above the bottom helix plate, and the end bearing capacity of the helical pile is measured. In the second stage, the hydraulic cylinder is assembled above the top helix plate, and the skin friction of the helical pile is measured. The pile loading-test program was carried out for the two different helical piles with the shaft diameter of 89 mm and 114 mm, respectively. However, the configuration of helix plates is identical with three helix plates of 450-, 350-, 200- mm diameter. Results of the bi-directional load test were verified by the conventional static pile loading test. As a result, the bearing capacity estimated by the bi-directional load test is in good agreement with the result of the conventional pile loading test.

• Journal of the Korean Geosynthetics Society
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• v.17 no.4
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• pp.29-40
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• 2018

Oil sands, which are largely distributed in Canada and Venezuela, are a mixture of crude oil and sandy soils. In order to extract crude oil from oil sands, construction of massive oil sand plants is required. Generally, the typically-used foundation types of the oil sand plant are driven piles and cast-in-place piles. Most of the oil sand plants are located in cold and remote regions. Installation of driven piles in frozen or organic surface soils is difficult due to high resistance and installation equipment accessability, while the cast-in-place pile has concrete curing problem due to cold temperature. Helical pile can be installed quickly and easily using rotation with a little help of vertical load. As the installation of helical pile is available using a small and light-weight installation equipment, accessibility of installation equipment is improved. The helical pile has an advantage of easy removal by rotation in reverse direction compared with that of installation. Furthermore, reuse of removed helical piles is possible when the piles are structurally safe. In this study, the behavior of helical piles varying helix pitch was analyzed based on the numerical analysis results. Numerical model was calibrated based on the results of model helical pile tests in laboratory. The ultimate helical pile loads, the displacement of each helix attached to the shaft of the helical pile, and the load sharing ratio of each helix were analyzed.

• The Journal of the Korea Contents Association
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• v.22 no.9
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• pp.194-204
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• 2022

This study examined the social capital and geographical gaps of local television stations using web data gathered through website crawling. URLs for 16 local MBC websites were collected. MBC is an abbreviation for Munhwa Broadcasting Corporation, one of South Korea's largest television and radio broadcasters. Munhwa is a Sino-Korean term that means "culture." It initially determined which institutions local broadcasting stations were linked to using a Web Impact Report. To investigate the specific connection type, URL information was classified using the n-tuple helix model, followed by 2-mode network analysis. The n-tuple helix model is an analysis method that extends the standard university-business-government triple-helix model by including a new network innovation originator. As a result, local broadcasting stations relied heavily on activities like as festivals, performances, and exhibitions to engage the local community. Local stations in Daegu-Gyeongbuk area and the Busan-Ulsan-Gyeongnam area were identified as having the most diverse connections to the local population among other regions.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.18 no.1
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• pp.99-132
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• 1992

Polypeptide has been used broadly as an active ingredient in cosmetics We thought it is very important to investigate the adsorption behavior of polypeptide in order to pre-estimate the effect of these polypeptides. For the study of polypeptide adsorption, we have investigated complex formation of basic homopolypeptides, poly(L-proline) Form I [PLP(I)], Form II [PLP(II)] and poly(4-hydroxy-L-proline) (PHLP) with acidic homopolypeptides, poly(L-glutamic acid) (PLGA), poly(D-glutamic acid) (PDGA) and poly(L-aspartic acid) (PLAA) through hydrogen bonding in a hydroalcoholic medium with viscometer, 1ight scatter, pH meter and circular dicroism (CD). The polypeptides used in this study have helical structure in some conditions. The result exhibited that al 1 the complexes were formed as the composition of basic/acidic homopolypeptide : L:2 irrespective of the complex systems used. A more favorable complex is formed in the PLP(II)-PLGA system than PHLP-PLGA because PLP(II) has a more flexible helical conformation, whereas PHLP has a more rigid helical conformation. The right-handed helix PLGA formed the complex favorably and quickly with the left-handed helix PLP(II), whereas the left-handed helix PDGA formed the complex favorably with the right-handed helix PLP(I). The effect of side chain of the acidic homopolypeptides on the complexation was also studied. The result showed that more favorable condition for the complexation was PLGA-PLP(II) system which has longer side chain at acidic homopolypeptide than PLAA - PLP(II). All the above facts were well supported by CD measurement for the complex systems. By the CD spectra for the complexes we could deduce the conformational change of each homopolypeptide in the complexes On the basis of the above results, we performed the adsorption test of PLP(I, II) and PHLP on the hair having a left-handed helix. The adsorption amount of each polypeptide was analyzed by HPLC. The result showed that PLP(I) was adsorbed more than PLP(II), PLP(II) was adsorbed more than PHLP on the hair. On adsorbing polypeptides having a helical structure on the hair through hydrogen bonding, it could be concluded that the helical polypeptides having the opposite directional structure to the hair are adsorbed more than those having the same directional structure with the hair and also the polypeptides having a flexible conformation are adsorbed more than those having a rigid conformation.