• BMB Reports
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• v.38 no.2
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• pp.243-247
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• 2005

Dishevelled (Dvl) is a positive regulator of the canonical Wnt signaling pathway, which regulates the levels of $\beta$-catenin. The $\beta$-catenin oncoprotein depends upon the association of Dvl and Axin proteins through their DIX domains, and its accumulation directs the expression of specific developmental-related genes at the nucleus. Here, the $^1H$, $^{13}C$, and $^{15}N$ resonances of the human Dishevelled 2 DIX domain are assigned using heteronuclear nuclear magnetic resonance (NMR) spectroscopy. In addition, helical and extended elements are identified based on the NMR data. The results establish a structural context for characterizing the actin and phospholipid interactions and binding sites of this novel domain, and provide insights into its role in protein localization to stress fibers and cytoplasmic vesicles during Wnt signaling.

• BMB Reports
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• v.38 no.2
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• pp.248-252
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• 2005

Dihydrolipoamide dehydrogenase (E3) catalyzes the reoxidation of dihydrolipoyl moiety of the acyltransferase components of three $\alpha$-keto acid dehydrogenase complexes and of the hydrogen-carrier protein of the glycine cleavage system. His-457 of Pseudomonas putida E3 is suggested to interact with the hydroxyl group of Tyr-18 of the other subunit and with Glu-446, a component in the last helical structure. To examine the importance of the suggested interactions in human E3 function, the corresponding residue of human E3, Asn-473, was substituted to Leu using site-directed mutagenesis. The E3 mutant was expressed in Escherichia coli and highly purified using an affinity column. Its E3 activity was decreased about 37-fold, indicating that Asn-473 residue was important to the efficient catalytic function of human E3. Its slightly altered spectroscopic properties implied that small conformational changes could occur in the E3 mutant.

• Journal of the Korean Magnetic Resonance Society
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• v.13 no.1
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• pp.56-63
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• 2009

SWIRM domain, a core domain of SRG3 is well conserved in SW13, RSC8, and MOIRA family proteins. To understand structural basis for cellular functions of the SWIRM domain, we have initiated biochemical and structural studies on SWIRM domain and mutants using gelfiltration chromatography, circular dichroism and NMR spectroscopy. The structural properties of the mutant SWIRM domains (K34A and M75A) have been characterized, showing that the structures of both wild-type and mutant proteins are a-helical conformation. The data conclude that mutations at interaction sites of its binding partner protein do not affect its secondary and tertiary structure.

• International Journal of Fluid Machinery and Systems
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• v.2 no.3
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• pp.239-247
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• 2009

Sewage pumps are designed with a wide flow channel by, for example, sacrificing some efficiency and reducing the number of blades, in order to prevent plugging with foreign bodies. However, the behavior of foreign bodies which actually flow into a pump is extremely complex, and there are questions about whether the presumed foreign bodies will actually pass through. This paper proposes a new type of sewage pump impeller designed to further improve pump efficiency and performance in passing foreign bodies. This sewage pump impeller has a structure in which the suction flow channel of a closed type non-clog pump is wound in a helical spiral. The focus of this research was to investigate pump performance and internal flow in this single blade sewage pump impeller. The results clearly indicated the following facts: The developed sewage pump impeller exhibits high efficiency over a wide range of flow rates; internal flow of the pump is very complicated; and the internal flow state varies greatly when the flow rate changes.

• Journal of the Korean Ceramic Society
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• v.35 no.7
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• pp.699-705
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• 1998

This paper is about design of optimal structure of microstrip dielectric ceramic antenna with rectangular electrode patches in accordance with the cavity model and fabrication of its prototype sample operating at the frequency of 900 MHz. Results of this work can be employed as a useful too to develop and diversify ceramic antenna having superior performance and omni-directivity to that of current helical antenna.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.33 no.4
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• pp.353-359
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• 2009

Wire-woven Bulk Kagome (WBK) is a new truss type cellular metal fabricated by systematic assembling of helical wires in six directions. In this work, the experiments of mechanical behaviors of WBK cored sandwich panels subjected to bending load were performed and the results were compared with those by the corresponding analytic solutions. And also, finite element simulations were performed to validate the optimal design according to the analytic solutions. It is found the sandwich panel with WBK core performed excellently in terms of energy absorption and deformation stability after the peak point as well as the load capacity.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.233-236
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• 2003

The micro-stereolithography technology made it possible to fabricate a freeform 3D micro-structure. Using this technology, two kind of applications were fabricated and tested: micro-lens and micro-mixers. The focal length and f-number of the micro-lens were 5 mm and 2.5, respectively. The focusing ability of the micro-lens was verified by defocusing the He-Ne laser beam after passing the lens. Two mixers are Kenics micro-mixer and BEKM. BEKM is a modified Kenics micro-mixer by introducing barriers on the pipe walt periodically to enhance the mixing via the chaotic mechanism in the helical flow of Kenics micro-mixer. Experimental result shows good mixing performance of developed mixers. Especially, the BEKM shows better mixing performance then Kenics micro-mixer.

• Journal of information and communication convergence engineering
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• v.7 no.3
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• pp.263-267
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• 2009

Cylindrical monopole antenna is one of most promising candidates for multi-band applications such as PCS, WLAN, DMB, and UWB wireless services. In this research, we demonstrate that there exist two types of current distributions according to the exciting frequency in a double band cylindrical monopole antenna, in which double resonance is achieved by adjusting the coupling structure of the antenna base. The operating modes of current distributions are identified from CST software simulations, the standing wave mode in a lower band and the traveling wave mode in a higher band. Also it is noticed that the mode behavior is quite similar to a helical antenna, a standing-wave (resonance) mode and a traveling-wave (non-resonance) mode according to the electrical dimensions of antenna. The effective ranges for operating modes and design formulas of the double band antenna are derived from simulation and measured results.

• Textile Coloration and Finishing
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• v.22 no.1
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• pp.37-42
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• 2010

ABS/MWNT nanocomposites were prepared by using MWNT wrapped with SAN through melt compounding. Effect of wrapping of MWNT by SAN on the morphology, mechanical and electrical properties of ABS/MWNT were analyzed. It was found that SAN could wrap MWNT effectively indicated by the increased thickness after wrapping, which is presumably due to helical structure of polyacrylonitrile component in a block copolymer of SAN. MWNT was observed to be dispersed more evenly in ABS matrix by SAN wrapping, which resulted in improved tensile properties of the composites. On the other hand, there was little effect on the impact strength and electrical properties of ABS having inherently high impact strength.

• Transactions of the Society of Information Storage Systems
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• v.10 no.1
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• pp.19-22
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• 2014

Optical nonlinear property is utilized to the wave generation, generating the beam at intended wavelength, and optical computing systems. The genetic material, which is the DNA with helical structure in nano-scale, is fascinating for optics communities due to artificially controllable sequence that determines the physical and chemical property. Nonlinearity of DNA was investigated by the four wave mixing experiment, which is with two incident beams located at 1550nm and 1650nm. The four output beams including incident beams are emitted from genetic material such as 1461nm and 1763nm by nonlinear characteristic. The 1461nm beam which is generated by four wave mixing phenomena was observed by optical spectrum analyzer.