• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.2
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• pp.1-6
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• 2019

The objective of this study was to investigate performance characteristics of thermal management system(TMS) in a fuel cell electric vehicle with 100kW Fuel Cell(FC) system. In order to build up analytic modelling for TMS, each component was installed and tested under various operating conditions, such as water pump, radiator, 3-Way valve, COD heater, and FC stack etc. and as the results of them, correlations reflecting component's characteristics with flow rate, air velocity were developed. Developed analytic modelling was carried out under various operating conditions on the road. To verify modelling's accuracy, after prediction for optimum coolant flow rate was fulfilled under certain operating conditions, such as FC system, water pump speed, opening of 3-way valve, and pipe resistance, analytic and experimental values were compared and good agreement was shown. In order to predict cold-start operating performance for analytic modelling, coolant temperature variation was analyzed with $-20^{\circ}C$ ambient temperature and duration was predicted to rise in optimum temperature for FC. Because there is appropriate temperature difference between inlet and outlet of FC stack to operate FC system properly, related analysis was performed with respect to power consumption for TMS and heat rejection rate and performance map was depicted along with FC operating conditions.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.5
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• pp.2519-2525
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• 2016

There are several existing reports of microarray chip use for assessment of altered gene expression in different diseases. In fact, there have been over 1.5 million assays of this kind performed over the last twenty years, which have influenced clinical and translational research studies. The most commonly used DNA microarray platforms are Affymetrix GeneChip and Quality Control Software along with their GeneChip Probe Arrays. These chips are created using several quality controls to confirm the success of each assay, but their actual impact on gene expression profiles had not been previously analyzed until the appearance of several bioinformatics tools for this purpose. We here performed a data mining analysis, in this case specifically focused on ovarian cancer, as well as healthy ovarian tissue and ovarian cell lines, in order to confirm quality control results and associated variation in gene expression profiles. The microarray data used in our research were downloaded from ArrayExpress and Gene Expression Omnibus (GEO) and analyzed with Expression Console Software using RMA, MAS5 and Plier algorithms. The gene expression profiles were obtained using Partek Genomics Suite v6.6 and data were visualized using principal component analysis, heat map, and Venn diagrams. Microarray quality control analysis showed that roughly 40% of the microarray files were false negative, demonstrating over- and under-estimation of expressed genes. Additionally, we confirmed the results performing second analysis using independent samples. About 70% of the significant expressed genes were correlated in both analyses. These results demonstrate the importance of appropriate microarray processing to obtain a reliable gene expression profile.

• Korean Journal of Materials Research
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• v.21 no.2
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• pp.95-99
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• 2011

The microstructure and Cu diffusion barrier property of Ta-Si-N films for various Si and N compositions were studied. Ta-Si-N films of a wide range of compositions (Si: 0~30 at.%, N: 0~55 at.%) were deposited by DC magnetron reactive sputtering of Ta and Si targets. Deposition rates of Ta and Si films as a function of DC target current density for various $N_2/(Ar+N_2)$ flow rate ratios were investigated. The composition of Ta-Si-N films was examined by wavelength dispersive spectroscopy (WDS). The variation of the microstructure of Ta-Si-N films with Si and N composition was examined by X-ray diffraction (XRD). The degree of crystallinity of Ta-Si-N films decreased with increasing Si and N composition. The Cu diffusion barrier property of Ta-Si-N films with more than sixty compositions was investigated. The Cu(100 nm)/Ta-Si-N(30 nm)/Si structure was used to investigate the Cu diffusion barrier property of Ta-Si-N films. The microstructure of all Cu/Ta-Si-N/Si structures after heat treatment for 1 hour at various temperatures was examined by XRD. A contour map that shows the diffusion barrier failure temperature for Cu as a function of Si and N composition was completed. At Si compositions ranging from 0 to 15 at.%, the Cu diffusion barrier property was best when the composition ratio of Ta + Si and N was almost identical.

• Journal of the Korean Magnetic Resonance Society
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• v.21 no.1
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• pp.31-43
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• 2017

Together with radiotherapy, chemotherapy using cytotoxic agents is one of the most common therapies in cancer. Metabolic changes in cancer cells are drawing much attention recently, but the metabolic alterations by anticancer agents have not been much studied. Here, we investigated the effects of commonly used cytotoxic agents on lung normal cell MRC5 and lung cancer cell A549. We employed cis-plastin, doxorubicin, and 5-Fluorouracil and compared their effects on the viability and metabolism of the normal and cancer cell lines. We first established the concentration of the cytotoxic reagents that give differences in the viabilities of normal and cancer cell lines. In those conditions, the viability of A549 decreased significantly, whereas that of MRC5 remained unchanged. To study the metabolic alterations implicated in the viability differences, we obtained the metabolic profiles using $^1H$-NMR spectrometry. The $^1H$-NMR data showed that the metabolic changes of A549 cells are more remarkable than that of MRC5 cells and the effect of 5-FU on the A549 cells is the most distinct compared to other treatments. Heat map analysis showed that metabolic alterations under treatment of cytotoxic agents are totally different between normal and cancer cells. Multivariate analysis and weighted correlation network analysis (WGCNA) revealed a distinctive metabolite signature and hub metabolites. Two different analysis tools revealed that the changes of cell metabolism in response to cytotoxic agents were highly correlated with the Warburg effect and Reductive lipogenesis, two pathways having important effects on the cell survival. Taken together, our study addressed the correlation between the viability and metabolic profiles of MRC5 and A549 cells upon the treatment of cytotoxic anticancer agents.

• 한국신재생에너지학회:학술대회논문집
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• 2009.06a
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• pp.608-610
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• 2009

지열 분포에 관련된 지질구조의 특성을 파악하기 위하여 지온경사, 지열류량 자료와 인접한 선구조의 방향성에 대한 상관성 분석을 실시하였다. GIS를 이용하여 지열정보데이터베이스에서 발췌한 209개 지온경사 자료와 218개 지열류량 자료에 대하여 전국 광역단열도로부터 가장 인접한 선구조를 추출하고 10도 간격의 방향별 지온경사 및 지열류량 자료 빈도 및 평균값을 그래프에 도시하였다. 인접 선구조의 방향별 지온경사 평균은 $N30^{\circ}{\sim}40^{\circ}W$, $N10^{\circ}{\sim}20^{\circ}E$ 방향에서 $58.9^{\circ}C/km$, $54.9^{\circ}C/km$로 가장 높았고, 인접 선구조의 방향별 지열류량 평균은 $N80^{\circ}{\sim}90^{\circ}W$, $N50^{\circ}{\sim}60^{\circ}W$ 방향에서 98.71 $mW/m^2$, $98.70mW/m^2$로 가장 높았다. 각 선구조 상의 좌표들을 이용하여 지열류량 분포도에서 지열류량 값을 추출한 결과 $N10^{\circ}W{\sim}N40^{\circ}E$, $N60^{\circ}{\sim}70^{\circ}W$, $N50^{\circ}{\sim}60^{\circ}E$ 방향의 지열류량 높은 것으로 나타났다. 결과적으로 지온경사와 지열류량 자료에 대한 인접 선구조의 방향은 북북동, 북서, 서북서 방향이 우세한 것으로 나타났다. 이는 우리나라 조구조 운동과 관련한 단층, 절리 등의 단열구조의 우세 방향과 잘 일치하는 것으로 해석된다.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.1
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• pp.78-84
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• 2017

Objective: The aim of this study was to examine shifts in the composition of the bacterial population in the intestinal tracts (ITs) of weaning piglets by antibiotic treatment using high-throughput sequencing. Methods: Sixty 28-d-old weaning piglets were randomly divided into two treatment groups. The Control group was treated with a basal diet without antibiotics. The Antibiotic group's basal diet contained colistin sulfate at a concentration of 20 g per ton and bacitracin zinc at a concentration of 40 g per ton. All of the pigs were fed for 28 days. Then, three pigs were killed, and the luminal contents of the jejunum, ileum, cecum, and colon were collected for DNA extraction and high-throughput sequencing. Results: The results showed that the average daily weight gain of the antibiotic group was significantly greater (p<0.05), and the incidence of diarrhea lower (p>0.05), than the control group. A total of 812,607 valid reads were generated. Thirty-eight operational taxonomic units (OTUs) that were found in all of the samples were defined as core OTUs. Twenty-one phyla were identified, and approximately 90% of the classifiable sequences belonged to the phylum Firmicutes. Forty-two classes were identified. Of the 232 genera identified, nine genera were identified as the core gut microbiome because they existed in all of the tracts. The proportion of the nine core bacteria varied at the different tract sites. A heat map was used to understand how the numbers of the abundant genera shifted between the two treatment groups. Conclusion: At different tract sites the relative abundance of gut microbiota was different. Antibiotics could cause shifts in the microorganism composition and affect the composition of gut microbiota in the different tracts of weaning piglets.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.20 no.5
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• pp.1591-1602
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• 1996

In operating the underwater engines such as encountered in exploring submarines, the dumping of the exhaust gas out of the engine requires a large portion of the total power, frequently amounting to 25-30% of the power generated. This unfavorable circumstance can be cured by liquefying the exhaust gas and storing it. In the present study, two liquefaction systems were simulated to enhance the overall efficiency; one is a closed cycle diesel engine and the other is a closed cycle LNG engine. The liquefied natural gas (LNG) is chosen as a fuel, not only because its use is economical but also because its cold energy can be utilized within the liquefaction system. Since a mixture of oxygen and carbon dioxide is used as an oxidizer, liquefying carbon dioxide is of major concern in this study. For further improving this system, the intercooling of the compressor is devised. The necessary power consumed for the liquefying system is examined in terms of the related properties such as pressure and temperature of the carbon dioxide vessel as a function of the amount of the exhaust gas which enters the compressor. The present study was successful to show that much gain in the power and reduction of the vessel pressure could be achieved in the case of the closed cycle LNG engine. The compression power of exhaust gas were observed remarkably lower, typically only 6.3% for the closed cycle diesel engine and 3.4% for the closed cycle LNG engine respectively, out of net engine power. For practicality, a design -purpose map of the operating parameters of the liquefaction systems was also presented.

• Journal of the Korean Society for Nondestructive Testing
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• v.28 no.2
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• pp.137-143
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• 2008

The spherical glasses lenses are typically classified into two groups such as (+) diopter lens and (-) diopter lens by the refractive power index. The thermal deformation of a lens is occurred by external heat source and is changed respected to the diopter of a lens. In this paper, the thermal deformation of spherical glasses lenses were quantitatively measured by using ESPI (electronic speckle pattern interferometry) which has an advantage that the non-contact, non-destructive and precise deformation measurement is available due to the coherency characteristic. The temperature changes were measured by IR camera. It makes experiments over 14 types of the plastic glasses lenses. From the results, it was confirmed that the larger diopter lens showed the less thermal deformation in case of the (+) diopter lens. On the other hand, the thermal deformation of the (-) diopter lens was measured with uniform pattern when the same temperature changes were applied. Also, it was found that the thermnal deformation of the (+) diopter lens is less than that of the (-) diopter lens. Therefore, it is expected that when the thermal deformation is occurred to the various types of the lens, the variation of the focal length caused by the thermal distortion of a lens would be measured quantitatively.

• Journal of Ginseng Research
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• v.42 no.1
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• pp.57-67
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• 2018

Background: Ginseng contains many small metabolites such as amino acids, fatty acids, carbohydrates, and ginsenosides. However, little is known about the relationships between microorganisms and metabolites during the entire ginseng fermentation process. We investigated metabolic changes during ginseng fermentation according to the inoculation of food-compatible microorganisms. Methods: Gas chromatography mass spectrometry (GC-MS) datasets coupled with the multivariate statistical method for the purpose of latent-information extraction and sample classification were used for the evaluation of ginseng fermentation. Four different starter cultures (Saccharomyces bayanus, Bacillus subtilis, Lactobacillus plantarum, and Leuconostoc mesenteroide) were used for the ginseng extract fermentation. Results: The principal component analysis score plot and heat map showed a clear separation between ginseng extracts fermented with S. bayanus and other strains. The highest levels of fructose, maltose, and galactose in the ginseng extracts were found in ginseng extracts fermented with B. subtilis. The levels of succinic acid and malic acid in the ginseng extract fermented with S. bayanus as well as the levels of lactic acid, malonic acid, and hydroxypruvic acid in the ginseng extract fermented with lactic acid bacteria (L. plantarum and L. mesenteroide) were the highest. In the results of taste features analysis using an electronic tongue, the ginseng extracts fermented with lactic acid bacteria were significantly distinguished from other groups by a high index of sour taste probably due to high lactic acid contents. Conclusion: These results suggest that a metabolomics approach based on GC-MS can be a useful tool to understand ginseng fermentation and evaluate the fermentative characteristics of starter cultures.

• The Journal of the Korean Society for Microbiology
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• v.35 no.4
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• pp.299-308
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• 2000

Recently developed cDNA microarray or DNA chip technology allows expression monitoring of expression of hundreds and thousands of genes simultaneously and provides a format for identifying genes as well as changes in their activity. In order to search for changes in gene expression after X irradiation in HL60 cells, cDNA microarray technique was done. In this study, expression of 588 human genes (including oncogenes, tumor suppressor genes, cell cycle regulator genes, intracellular signal transduction modulator genes, apoptosis related genes, transcription factor genes, growth factors and receptor genes, cytokine genes, etc) were analyzed. For cDNA microarray analysis mRNAs were extracted from control and 8 Gy-irradiated HL60 cells. As a result the changes in expression of several genes were observed. This alteration of gene expression was confirmed by reverse transcription-polymerase chain reaction. The expression of heat shock 60 KD protein, c-jun, erythroid differentiation factor, CPP32, myeloid cell nuclear differentiation antigen, MAP kinase-activated protein kinase, interleukin-8, monocyte chemotactic peptide 1 and RANTES genes was increased, but the expression of p55CDC gene was decreased after X irradiation.