• 제목/요약/키워드: heat exposure

Search Result 542, Processing Time 0.034 seconds

Effect of Dry Heat Treatment on Seed Germination and Seedling Growth in Watermelon (수박종자의 건열 처리가 발아 및 유묘생장에 미치는 효과)

  • Eun-Ji Park;Jung-Eun Lee;Seong-Kwang An;Byoung-Il Je;Young-Hoon Park;Yong-Jae Lee;Young-Whan Choi;Jum-Soon Kang
    • Journal of Environmental Science International
    • /
    • v.32 no.2
    • /
    • pp.121-130
    • /
    • 2023
  • This study was conducted to investigate changes in seed vigor based on temperature of dry heat and duration treatment of watermelon seeds and examine the effect on percent of emergence and seedling vigor. When the upper limit temperature of dry heat treatment was raised to 80℃, the percent of the germination decreased. Moreover, T50 was delayed as the upper limit temperature of dry heat treatment increased. The higher the upper limit temperature of dry heat treatment and the longer the treatment period, the higher the percentage of abnormal seedlings. The optimum upper limit temperature for dry heat treatment was 72℃, and the treatment period was five days. Seed vigor was better maintained at 30℃, 45℃, and 52℃, followed by stepwise exposure to high temperatures of 72℃, the upper limit of dry heat treatment, rather than dry heat treatment at a high temperature of 72℃ for 5 days from the initial stage of treatment. When the fungicide was added during the dry heat treatment process, the germination percentage decreased and the percent of the abnormal seedling percentage increased. However, the addition of 10 mg/kg fungicide did not significantly reduce seed vigor.

Shear Characteristics of Elastomeric Bearing Rubber Deteriorated by Accelerated Heat Aging(1): Natural Rubber (가속열 노화로 열화된 탄성받침 고무재료의 전단 특성(1): 천연고무)

  • Sun, Chang-Ho;Kim, Ick-Hyun
    • Journal of the Korea institute for structural maintenance and inspection
    • /
    • v.25 no.6
    • /
    • pp.95-102
    • /
    • 2021
  • Elastometic bearings using flexible rubber materials have recently been widely applied for seismic retrofit of bridges. However, due to various factors, the aging of the rubber material progresses, which causes the shear stiffness change of the bearing, which affects the seismic performance of the bridge. For natural rubber, accelerated heat aging test was performed with variables of heating temperatures and exposure time to analyze shear characteristics. As aging progresses (i.e. increase of temperature and exposure time), the maximum shear stress and shear strain decrease. Also, the shear stiffness is greatly increased at the same shear strain. This means that the rubber material is hardened, implying that the seismic performance of the elastomeric bearing becomes poor.

Isolation and Characterization of a cDNA Encoding Two Novel Heat-shock Factor OsHSF6 and OsHSF12 in Oryza Sativa L.

  • Liu, Jin-Ge;Yao, Quan-Hong;Zhang, Zhen;Peng, Ri-He;Xiong, Ai-Sheng;Xu, Fang;Zhu, Hong
    • BMB Reports
    • /
    • v.38 no.5
    • /
    • pp.602-608
    • /
    • 2005
  • As a crucial transcription factor family, heat-shock factors were mainly analyzed and characterized in tomato and Arabidopsis. In this study, we isolated two putative heat shock factors OsHSF6 and OsHSF12 that interact specifically with heat-shock element (HSE) from Oryza sativa L by yeast one-hybrid method. The full-length cDNA of OsHSF6 and OsHSF12 have 1074bp and 920bp open reading frame (ORF), respectively. Analysis of the deduced amino acid sequences revealed that OsHSF6 was a class A heat shock factor (HSF) with all the conserved sequence elements characteristic of heat stress transcription factor, while OsHSF12 was a class B HSF with C-terminal domain (CTD) lacking of AHA motif. Bioinformatic analysis showed that the sequences and structures of two HSFs' DNA binding domain (DBD) had a high similarity with LpHSF24. The results of RT-PCR indicated OsHSF6 gene was expressed immediately after rice plants exposure to heat stress, and the transcription of OsHSF6 gene accumulated primarily in immature seeds, roots and leaves. However, we did not find the transcription of OsHSF12 gene in different organs and growth periods. Our results implied that OsHSF6 might be function as a HSF regulating early expression of stress genes in response to heat shock, and OsHSF12 might be act as a synergistic factor to regulate the expression of down-stream genes.

Korean Red Ginseng and Korean black ginseng extracts, JP5 and BG1, prevent hepatic oxidative stress and inflammation induced by environmental heat stress

  • Song, Ji-Hyeon;Kim, Kui-Jin;Chei, Sungwoo;Seo, Young-Jin;Lee, Kippeum;Lee, Boo-Yong
    • Journal of Ginseng Research
    • /
    • v.44 no.2
    • /
    • pp.267-273
    • /
    • 2020
  • Background: Continuous exposure to high temperatures can lead to heat stress. This stress response alters the expression of multiple genes and can contribute to the onset of various diseases. In particular, heat stress induces oxidative stress by increasing the production of reactive oxygen species. The liver is an essential organ that plays a variety of roles, such as detoxification and protein synthesis. Therefore, it is important to protect the liver from oxidative stress caused by heat stress. Korean ginseng has a variety of beneficial biological properties, and our previous studies showed that it provides an effective defense against heat stress. Methods: We investigated the ability of Korean Red Ginseng and Korean black ginseng extracts (JP5 and BG1) to protect against heat stress using a rat model. We then confirmed the active ingredients and mechanism of action using a cell-based model. Results: Heat stress significantly increased gene and protein expression of oxidative stress-related factors such as catalase and SOD2, but treatment with JP5 (Korean Red Ginseng extract) and BG1 (Korean black ginseng extract) abolished this response in both liver tissue and HepG2 cells. In addition, JP5 and BG1 inhibited the expression of inflammatory proteins such as p-NF-κB and tumor necrosis factor alpha-α. In particular, JP5 and BG1 decreased the expression of components of the NLRP3 inflammasome, a key inflammatory signaling factor. Thus, JP5 and BG1 inhibited both oxidative stress and inflammation. Conclusions: JP5 and BG1 protect against oxidative stress and inflammation induced by heat stress and help maintain liver function by preventing liver damage.

Preparation of Photocurable Slurry for DLP 3D Printing Process using Synthesized Yttrium Oxyfluoride Powder (합성 불산화 이트륨 분말을 이용한 DLP 3D 프린팅용 광경화성 슬러리 제조)

  • Kim, Eunsung;Han, Kyusung;Choi, Junghoon;Kim, Jinho;Kim, Ungsoo
    • Korean Journal of Materials Research
    • /
    • v.31 no.9
    • /
    • pp.532-538
    • /
    • 2021
  • In this study, a spray dryer is used to make granules of Y2O3 and YF3, and then Y5O4F7 is synthesized following heat treatment of them under Ar gas atmosphere at 600 ℃. Single and binary monomer mixtures are compared and analyzed to optimize photocurable monomer system for DLP 3D printing. The mixture of HEA and TMPTA at 8:2 ratio exhibits the highest photocuring properties and low viscosity with shear thinning behavior. The optimized photocurable monomer and synthesized Y5O4F7 are therefore mixed and applied to printing process at variable solid contents (60, 70, 80, & 85 wt.%) and light exposure times. Under optimal light exposure conditions (initial exposure time: 1.2 s, basic exposure time: 5 s), YOF composites at 60, 70 & 80 wt.% solid contents are successfully printed. As a result of measuring the size of the printed samples compared to the dimensions of the designed bar type specimen, the deviation is found to increase as the YOF solid content increases. This shows that it is necessary to maximize the photocuring activity of the monomer system and to optimize the exposure time when printing using a high-solids ceramic slurry.

Heat Shock Causes Oxidative Stress and Induces a Variety of Cell Rescue Proteins in Saccharomyces cerevisiae KNU5377

  • Kim, Il-Sup;Moon, Hye-Youn;Yun, Hae-Sun;Jin, Ing-Nyol
    • Journal of Microbiology
    • /
    • v.44 no.5
    • /
    • pp.492-501
    • /
    • 2006
  • In this study, we attempted to characterize the physiological response to oxidative stress by heat shock in Saccharomyces cerevisiae KNU5377 (KNU5377) that ferments at a temperature of $40^{\circ}C$. The KNU5377 strain evidenced a very similar growth rate at $40^{\circ}C$ as was recorded under normal conditions. Unlike the laboratory strains of S. cerevisiae, the cell viability of KNU5377 was affected slightly under 2 hours of heat stress conditions at $43^{\circ}C$. KNU5377 evidenced a time-dependent increase in hydroperoxide levels, carbonyl contents, and malondialdehyde (MDA), which increased in the expression of a variety of cell rescue proteins containing Hsp104p, Ssap, Hsp30p, Sod1p, catalase, glutathione reductase, G6PDH, thioredoxin, thioredoxin peroxidase (Tsa1p), Adhp, Aldp, trehalose and glycogen at high temperature. Pma1/2p, Hsp90p and $H^+$-ATPase expression levels were reduced as the result of exposure to heat shock. With regard to cellular fatty acid composition, levels of unsaturated fatty acids (USFAs) were increased significantly at high temperatures ($43^{\circ}C$), and this was particularly true of oleic acid (C18:1). The results of this study indicated that oxidative stress as the result of heat shock may induce a more profound stimulation of trehalose, antioxidant enzymes, and heat shock proteins, as well as an increase in the USFAs ratios. This might contribute to cellular protective functions for the maintenance of cellular homeostasis, and may also contribute to membrane fluidity.

Antioxidants Supplementation on Acid Base Balance during Heat Stress in Goats

  • Sivakumar, A.V.N.;Singh, G.;Varshney, V.P.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.23 no.11
    • /
    • pp.1462-1468
    • /
    • 2010
  • The effects of vitamin C and vitamin E with selenium on acid-base balance and some stress hormones were evaluated during heat stress in goats. Goats, 1.5 years of age, were divided into control, heat stress and antioxidant treatment groups 1, 2 and 3. Except for the control, all groups were exposed to a temperature of $40{\pm}2^{\circ}C$ with a relative humidity of 30% for 5 h/d for 21 days in a psychrometric chamber. Rectal temperature and respiratory rates were recorded daily post exposure. Blood samples were collected on every 3rd day for estimation of plasma vitamins C and E, total antioxidant activity and hormones, and separate blood samples were taken to estimate acid-base status. The rectal temperature and respiratory rates were increased (p<0.05) in the heat stress group only. Except for pH and $pO_2$, which were increased significantly (p<0.05) other parameters of acid-base balance such as $pCO_2$, $HCO_3^-$, $TCO_2$, BEb, BEcef, PCV and Hb were significantly decreased (p<0.05) in the heat stress group. An improvement in acid-base status was noted in the antioxidant supplemented groups. Prolactin and cortisol levels were significantly (p<0.05) higher and free T3 and T4 levels were significantly (p<0.05) lower in the heat stress group. Levels of prolactin and cortisol were decreased and free T3 and T4 were increased in antioxidant treatment groups. Different levels of antioxidant supplementation resulted in similar protection against heat stress.

A Case Study on the Release Characteristic and Removal Efficiency of Vinyl Chloride in the Poly Vinyl Chloride Extrusion Process (PVC압출공정의 염화비닐 발생특성과 작업환경개선에 관한 사례연구)

  • Park, Dong Uk
    • Journal of Korean Society of Occupational and Environmental Hygiene
    • /
    • v.3 no.1
    • /
    • pp.91-98
    • /
    • 1993
  • This study was carried out to investigate characteristic of vinyl chloride emissioned from poly vinyl chloride extrusion process and to evaluate the efficiency of local exhaust ventilation system. Before local ventilation facility was constructed in poly vinyl chloride extrusion process, the average worker exposure to vinyl chloride was 3.15 ppm, which exceeded Threshold Limit Value of American Conference of Gorvernmental Industrial Hygienists (ACGIH-TLV), 1 ppm. lt is possible that vinyl chloride residues in the poly vinyl chloride resin was released or degased due to extrusion heat. The larger the width of vinyl tube become, the higher worker exposure to vinyl chloride was. It is estimated that vinyl chloride from vinyl chloride resin increased as amount of poly vinyl chloride resin extruded in the extrusion process increased. Canopy hood was an appropriate type for poly vinyl chloride resin extrusion process. This local exhaust ventilation has fan static pressure of 7.65 inch wg($190mmH_2O$, total volumetric flowlate of 4,796 CFM ($135.8m^3$/min) and fan power requirement of 12 hp (8.952 Kw). After this local exhaust ventilation was constructed there, the average concentration of worker exposure to vinyl chloride was reduced to be 0.46 ppm, which was below the Threshold Limit Value, 1 ppm. Also, the removal efficiency rate of vinyl chloride attained by local exhaust ventilation was 85.3%. It was a statistically significant (p<0.01).

  • PDF

Postfire reliability analysis of axial load bearing capacity of CFRP retrofitted concrete columns

  • Cai, Bin;Hao, Liyan;Fu, Feng
    • Advances in concrete construction
    • /
    • v.10 no.4
    • /
    • pp.289-299
    • /
    • 2020
  • A reliability analysis of the axial compressive load bearing capacity of postfire reinforced concrete (RC) columns strengthened with carbon fiber reinforced polymer (CFRP) sheets was presented. A 3D finite element (FE) model was built for heat transfer analysis using software ABAQUS. Based on the temperature distribution obtained from the FE analysis, the residual axial compressive load bearing capacity of RC columns was worked out using the section method. Formulas for calculating the residual axial compressive load bearing capacity of the columns after fire exposure and the axial compressive load bearing capacity of postfire columns retrofitted with CFRP sheets were developed. Then the Monte Carlo method was used to analyze the reliability of the axial compressive load bearing capacity of the RC columns retrofitted with CFRP sheets using a code developed in MATLAB. The effects of fire exposure time, load ratio, number of CFRP layers, concrete cover thickness, and longitudinal reinforcement ratio on the reliability of the axial compressive load bearing capacity of the columns after fire were investigated. The results show that within 60 minutes of fire exposure time, the reliability index of the RC columns after retrofitting with two layers of CFRPs can meet the requirements of Chinese code GB 50068 (GB 2001) for safety level II. This method is effective and accurate for the reliability analysis of the axial load bearing capacity of postfire reinforced concrete columns retrofitted with CFRP.

Development of a jet air supplying welding mask for controlling welding fumes (압축공기를 이용한 용접흄 제어용 용접면(JASM)의 개발)

  • Song, Se-Wook;Kim, Jong-Gil;Ha, Hyun-Chul;Kim, Tae-Hyeung;Kim, Jong-Chul;Jung, Yu-Jin
    • Journal of Korean Society of Occupational and Environmental Hygiene
    • /
    • v.10 no.2
    • /
    • pp.98-108
    • /
    • 2000
  • Controlling the over-exposure of welding fumes is not an easy problem because neither general nor local exhaust ventilation systems could be successfully applied. A jet air supplying welding mask was development to reduce the exposure level of welding fumes. The jet airs tream pushes the welding fumes away from the breathing zone by using the frictional characteristic of jet. Laboratory experiments were conducted to optimize the efficiency of controlling welding fumes. Thereafter, its performance was tested in a laboratory and an industrial field. The efficiencies of reducing the welding fume exposure were about 90% and 80% in a laboratory and an industrial field, respectively. Additionally, it resulted in elimination of heat inside the mask and enhancement of clear visuality.

  • PDF