• 제목/요약/키워드: health education needs

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Borich 요구도와 The Locus for Focus Model을 이용한 전북지역 학교급식 조리종사자의 안전사고 예방관리를 위한 교육요구도 우선순위 분석 (An educational needs analysis of precautions against of safety accidents for school foodservice employees in the Jeonbuk area using Borich priority formula and the Locus for Focus Model)

  • 이향진;최선아;노정옥
    • Journal of Nutrition and Health
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    • 제56권5호
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    • pp.554-572
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    • 2023
  • 본 연구는 전북지역 316명의 초·중·고등학교 조리종사자를 대상으로 안전사고 실태 및 예방교육 프로그램에 활용될 교육내용의 우선순위를 조사하였으며, 그 결과를 요약하면 다음과 같다. 조사대상자의 99.7%는 '여자', 학력은 '고졸' 76.6%, 직급은 '조리원' 77.8%, 평균 근무연수는 초등학교 '12.7년', 중·고등학교 '10.6년'이었다 (p < 0.01). 1일 평균 급식 인원은 초등학교 '716.4명', 중·고등학교 '818.3명'이며 (p < 0.05), 평균 근무인원은 초등학교 '6.1명', 중·고등학교 '8.0명'이었다 (p < 0.001). 조리종사자의 90.8%가 안전사고를 경험하였으며, 중·고등학교 종사자가 초등학교보다 사고경험이 많았다. 주요 사고원인은 '과도한 업무량' (61.7%), '노후기구나 설비' (28.9%), '본인 부주의' (17.1%)이며, 주로 '조리과정' (47.7%), '청소 및 뒷정리' (42.2%), '이동 및 운반' (36.2%) 과정에서 발생하였다. 가장 많은 부상은 초등학교는 '근골격계부상' (68.2%), 중·고등학교는 '미끄러짐' (70.2%)과 '화상' (70.2%)이었다. 안전교육은 영양(교)사와 교육청 (100%), 조리사협회 (27.2%)의 교육을 받았으나 초등학교의 77.3%, 중·고등학교의 79.5%의 조리종사자는 교육내용을 현장에 적용하지 않았다. 안전교육에 대하여 64.9%의 조리종사자는 만족, 35.1%는 불만족하며, 가장 큰 불만족 이유는 '교육내용이 실제 급식현장과 맞지 않아서' (42.3%)이었다. 안전교육 내용의 우선순위 결정을 위해 대응표본 t-test, Borich 요구도, The Locus for Focus 모델의 3단계 분석방법을 이용한 결과, 6개 영역의 22개 항목 중 1항목 '작업장 및 통로를 정리·정돈한다'를 제외한 21개 항목에서 중요도와 현재 수행수준 간에 유의미한 차이가 있었다. 안전교육 내용에 대한 초등학교와 중·고등학교 종사자의 최우선 순위는 2개항목, 차 순위는 6항목으로 확인되었다. 따라서 초등학교와 중·고등학교 조리종사자 대상 안전교육 시 우선적으로 '가스후드 청소 시 안전한 사다리를 사용하며, 2인 1조로 작업한다', '배수로 청소 시에는 주의 표시판을 설치하고, 청소 후에는 반드시 덮개를 덮는다'와 '화학물질 취급 전 보호장비를 착용한다'를 교육하여야 하며, 차 순위로는 '작업 전·후 스트레칭 하기', '작업대 높이가 맞지 않으면 보조 받침대를 사용하여 높이를 맞춘다', '무거운 것을 옮길 때 보조기구를 이용하거나 도움을 받는다', '중량물 취급 시에는 올바른 자세와 방법을 지킨다', 'MSDS 적용물질의 안전취급 요령을 잘 확인하고 사용한다'가 교육내용에 포함되어야 되겠다. 이상의 결과, 조리종사자 안전교육 시 조리, 청소 및 뒷정리 과정 중의 부딪힘·떨어짐 사고, 화학물질관리 및 근골격계질환 사고 예방교육을 우선적으로 하여야 하겠다. 본 연구의 제한점은 연구대상자가 일부 지역에 국한되어 있기 때문에 전체 학교급식소의 경우로 일반화하기에는 한계가 있으며 향후 조사대상자의 확대를 통하여 전체 조리종사자의 안전사고 예방교육을 위한 체계수립이 필요하겠다.

치과의료 재이용 및 권유의사 관련 요인 (Factors upon revisit and intention of recommendation for dental care service)

  • 이향님;심형순
    • 한국치위생학회지
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    • 제12권2호
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    • pp.317-326
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    • 2012
  • Objectives : This study aims to examine satisfaction of dental treatment and revisit and intention of recommendation to dental patients and to supply as a basic resource for dental management improvement. Methods : 645 dental patients to dental hospitals in G City were subjects of this study. 27 dental hospitals were randomly chosen per 5 or 6 in 5 Districts. The intention of the survey was explained to 645 patients and it was agreed to have questionnaire survey. Questionnaire survey was carried out for 2 weeks in April 2011. Total 593 were used for the analysis except for insufficient answer sheets. The analysis was executed by using SPSS 18.0 and the results are as follows. Results : 1. The highest figures are as follows: female was 53.29% in sex, 20-29 age group was 44.52%, graduates of university were 38.62%, students were 28.50%, monthly family income with 300-399 million won was 31.70%. As to the treatment, dental caries were highest with 46.21% followed by scaling with 40.81%. As to the number of visit, 39.80% were over 5 times, and as to the opportunity to use, 29.34% answered it because it is close to home. 2. As to satisfaction, male was 4.15, over 60years old was 4.58, self-employed was 4.33, friendship with dentist as opportunity to use was 4.37. And as to revisit intention, over 60 years old was 4.60, over postgraduate in education level was 4.38, self-employed was 4.43, over 5 times visit was 4.32, and fame as opportunity to use was 4.39 which were the highest. As to the intention of recommendation to others, female was 4.24, over 60 years old was 4.65, self-employed was 4.36, friendship with dentist as opportunity was 4.43 which were the highest and showed significant differences (p<0.05). 3. As to patient satisfaction, there were strong relevance between revisit intention and intention of recommendation with 0.769 and 0.744 respectively. And, as to revisit intention, it had significant relation with revisit intention with 0.791. 4. The explanatory power of the regression analysis on the factors affecting revisit intention was 61.20%. The revisit intention was high when the satisfaction of dental hospital was high (${\beta}$=0.767, p<0.001), the number of visit (${\beta}$=0.026, p<0.01), reason for a visit (${\beta}$=0.070, p<0.01), education level (${\beta}$=-0.063, p<0.05) and occupation (${\beta}$=0.078, p<0.05). 5. The explanatory power of the regression analysis on the factors affecting intention of recommendation was 59.9%. The intention was taken patients satisfaction(${\beta}$=0.601, p<0.001), dental hygienist's kindness (${\beta}$=0.218, p<0.001), female (${\beta}$=0.079, p<0.05), reason for a visit(${\beta}$=-0.059, p<0.05), dissatisfaction treat(${\beta}$=0.084, p<0.05), dentist's kindness (${\beta}$=-0.080, p<0.05), age (${\beta}$=0.054, p<0.05). Conclusions : To improve revisit rate of patients, the refurbishing hospital facility, treatment and kindness of dental hygienists and satisfaction of treatment must be improved. And it also needs to accept complaint from female patients in a positive manner, and understand and correct complaints actively from the perspective of patients.

노후적응에 관한 연구 - 생활만족도 및 가족의 교류도를 중심으로- (A study on the adjustment problems of the aged)

  • 서병숙
    • 대한가정학회지
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    • 제27권2호
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    • pp.133-148
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    • 1989
  • This study attempts of analyze empirically the living status of the elderly in Korea by finding out the problems of it in the family and by clarifying the influences of the family environmental variables and the values on the adjustment of the aged. To carryout the aim of this study, the causes and the patterns of the problems for the aged had been found out through the review of literature. Based on them the questionnaire was prepared including the questions of the values, family environmental vriables as well as the degree of satisfaction of thier liver and the extent of life interchange. The former variables were taken as independent and the latter were taken as dependent variables, so that the relationship between the two variables could be exemplified. The 400 aged over 60 living in Seoul and the other cities were selected through the purposive sampling and interviewed. The answers of the 376 respondents, 94% of the sample, were analyzed for this study. The following results have been found out through the various statistical analysis such as frequency distribution, chi-square test, pearson's correlation, analysis of variance and cluster analysis. At first, the results from the examination of the frequency distribution regarding the living status of the aged are; I) sixteen percent of the respondents living with their married offspring were receiving economical support, though they pointed out the problems of the extended family are the inconveniences with their children (60%) and simpleness (20%) of their livers. It seems that the aged in Korea are poor and have negative opinion on the relationship with their offspring even if they live in the same house. Secondly, it has been found out that the family environmental variables, especially such as sex, occupation, residence, education, level, living ability and the family type, play and important role not only on the degree of satisfaction of their liver but also on the extent of interchange of their lives. However, the variables such as religion and whether the spouse is alive or not do not play and important role. Thirdly, the reported main reasons of their delightness were healthy life (1.87%), the well-being of their offspring (2.08%), the spouse's long life (3.38%), religious activity (5.05%), the hobby activity (4.31%) and the participation in the social activity (5.05%). While, they mainly concern about the illness (1.98%), offspring (2.00%), econmic difficulties (3.39%), inharmonious home (3.81%) and the despicable treatment (3.81%). The respondents wer classified into two groups, they are dependent and independent, using cluster analysis. Health, social activity, offspiring, religion, couple's long life among the factors which lead them to be delighted were turned out to be statistically signhificant at 0.01 significance level between the two groups. As far as their worrisome matters are concerned, health and economic difficulties were significantly different between the two groups. Fourthly, regarding the life interchange, it has been proved that there is a statistically significant differences betwwen the two groups in the economic and the emotional satisfaction, help for ordinary life, status in the family, subjective and objective relationship with offspring, but not in economic dependency and the activity status. Fifthly, it has been noted that there is a high correlation between the degree of life's satisfaction and the extent of life interchange. On the basis of the results mentioned above, it could be concluded taht; i) The majority of the aged in Korea are not economically active, and thus they live with their married offspring even of they have a negative opinion on the subjective relationship with them. This can be pointed out as the main problem of the aged and thus it needs to be studied further. ii) the environmental variables such as age, sex education level, family type and occuption (which can not be changed by their effrot) turned out to give an important effect on the extent of the life interchange among the aged. iii) The respondents who are identified as independent group have a higher degree of life satisfaction and family status than the group identified as defendent. Thus, it can be inferred that it is possible for them to adjust their life by making an effort to modify themselves. iv) The high correlation between the extent of life interchange and the degree of life satisfaction indicates that the elderly themselves can adjust in order to obtain the higher degree of life satisfaction. Therefore, the following suggestions are derived from this study. First of all, the aged should try to be psychologically independent. Second, they need to control themselves in order to achieve self-assurance. Third, the community have to prepare a program of self-development for the aged. Fourth, the social welfare policy that can solve the problem of illness and poverty of the elderly should be introduced, so that their minimum requirements can be satisfied.

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일부 시 지역 고교생.학부모.교사들의 성의식 조사 (A Research Study on the Sexual Awareness of Teenagers. Their Parents and Teachers in an Urban Area)

  • 임미림;양순옥;이광옥
    • 지역사회간호학회지
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    • 제12권3호
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    • pp.755-772
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    • 2001
  • The specific purpose of this study was as follows: 1) To identify the level of sexual awareness and demand for sex education of teenagers, their parents and teachers. 2) To investigate the attitude toward and acceptance of heterosexual friendships by teenagers, their parents and teachers. 3) To identify the experience and direction of sex education of parents and teachers. For this purpose, this study selected 341 male and female teenagers at three high schools located in a city, 119 parents with high school students and 243 elementary/middle/high school teachers. The structured questionnaire was distributed to each of them and returned by them. This study was conducted over the period between June 1. 2001 and Aug 31. 2001. A total of 720 questionnaires were distributed to them and 703 were returned. Data analysis was conducted to obtain percentage and frequency using the SAS program. As a result, the following findings were obtained: Parents and teachers showed a similar attitude about sexual awareness, but teenagers showed a different attitude. In terms of the level of feeling of and interest in 'sex', teenagers, parents and teachers were comfortable in accepting it. As for sexual curiosity in puberty, 84.8% of the parents and 95.5% of the teachers responded that it was natural, whereas only 14.7% of teenagers responded that it was natural. But As for the item that 'sex is natural as one of human needs', 3.6% of the teenagers, 16.8% of the parents and only 1.2% of the teachers responded that it was not. As for the item that 'A man has a stronger sexual impulse than a woman', 6.1% of the teenagers, 16.8% of the parents and 17.3% of the teachers responded that it was not. 64.2% of the teenagers and 90.2% of the teachers responded that it was impossible to overcome the sexual impulse, whereas only 8.4% of the parents responded that it was possible. As for the item of masturbation, 64.5% of the teenagers responded that it was possible for male students and not possible for female students. 67.2% of the parents and 91.1% of the teachers responded that it was possible regardless of sex. As for the item of an attitude toward virginal purity, both the parents and teachers showed that virginal purity was important for both man and woman, but teenagers showed a weak awareness of virginal purity. As for the item, 'It is possible to have sexual intercourse with a lover during adolescence', 20.2% of the teenagers, 79% of the parents and 87.7% of the teachers responded that it was impossible. As for the item 'It is necessary to keep premarital virginal purity for a happy married life', 21.1% of the teenagers, 74.8% of the parents and 55.1% of the teachers responded that it was necessary, which shows that teenagers had a different opinion than parents and teachers. In case of pregnancy in adolescence, most of the responding teenagers, parents and teachers chose abortion. As for the item 'female refusal on male sexual approach', respondents accepted female refusal as they did for orders of teachers, parents and adolescents. As for item 'An assaulter is entirely to blame for sexual assault', all of three groups responded in the affirmative.

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한국 커뮤니티 댄스의 효과와 역할 (Effects and Roles of Korean Community Dance)

  • 박소정
    • 트랜스-
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    • 제9권
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    • pp.37-66
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    • 2020
  • 21세기로 접어들면서 사회와 문화의 흐름은 자기 스스로 경험하고 즐기고 체험을 하는 단지 보고 즐기는 것에서 자신이 직접 경험하고 행위의 주체 자가 되는 문화현상으로 나타나고 있다. 이러한 흐름은 2010년부터 활성화되고 있는 커뮤니티 댄스(Community Dance)로 나타났다. 커뮤니티 댄스는 누구나 대상이 될 수 있으며 모임의 특성과 연령에 따라 아동 무용, 성인 무용, 노인무용으로 나뉠 수 있어 다양한 연령대로 활동 할 수 있다. 또한 미취학 아동부터 노인에 이르기까지 연령대별로 모임에서 성별, 인종, 종교에 관계없이 건강을 증진하거나 표현의 욕구를 충족하고 체력을 향상시킬 수 있는 모든 사람의 행복과 자아 성취를 위한 모든 종류의 춤을 말한다. 모든 사람들이 각자의 여가시간을 활용하여 자발적으로 즐겁게 참여하는 무용 활동으로 체험을 통한 춤이라는 비언어적 특징이 세대 간의 공감대와 소통을 이루며 삶의 질 향상에 도움을 줄 수 있다. 커뮤니티 댄스는 평생교육을 통해 사회학습으로의 확장성을 가져오며 전문가들이 일반인들을 대상으로 함께 진행하는 자발적인 공동체 모임이다. 커뮤니티 댄스의 정의는 누구나 개인의 일상생활을 풍요롭게 하고 사회적으로 공동체의식을 높여 밝은 사회를 만들며, 개개인에게는 건강증진과 미적 교육의 목표를 이루는 신체활동의 총체라고 할 수 있다. 또한 창의성의 의미를 자기표현으로서의 몸과 창의성에서 무용체험은 참여자가 참여 과정에서 경험하는 긍정적인 심리체험과 일상에 미친 영향력을 탐색해 행복 관점을 투영하여 커뮤니티 댄스의 효과로 나타나고 있으며 참여자들은 춤 문화를 향유를 위해 지금까지 온라인 오프라인을 통해 지속적인 네트워킹을 하고 있다. 커뮤니티 댄스의 붐이 불기 시작하면서 10년간 다양한 분야로 연구가 진행되었지만 실질적인 프로그램의 방법론은 미비하여 국내에서 진행한 커뮤니티 댄스 사례를 조사하여 펠든크라이스 기법(Feldenkrais Method)을 제시하여 향 후 국내 커뮤니티 댄스에 필요한 방법론으로 자리 잡아 문화 소외계층과 활용될 수 있기를 기대하며 무용의 신체 기능적 측면의 향상과 심리적 안정감을 줄 수 있는 교육적 효과와 예술가들의 안무 창작법의 일환으로 활용될 수 있으리라 사료된다.

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독립형 호스피스 센터 모델 개발에 관한 연구 (A Study on the Development of an Independent Hospice Center Model)

  • 노유자;한성숙;김명자;유양숙;용진선;전경자
    • 대한간호학회지
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    • 제30권5호
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    • pp.1156-1169
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    • 2000
  • The study was aimed at developing an independent hospice center model that would be best suited for Korea based on a literature review and the current status of local and international hospices. For the study, five local and six international hospice organizations were surveyed. Components of the hospice center model include philosophy, purpose, resources (workers, facilities, and equipment), allocation of resources, management, financial support and hospice team service. The following is a summary of the developed model: Philosophies for the hospice center were set as follows: based on the dignity of human life and humanism, help patients spend the rest of their days in a meaningful way and accept life positively. On the staff side, to pursue a team-oriented holistic approach to improve comfort and quality of life for terminally ill persons and their families. The hospice center should have 20 beds with single, two, and four bed rooms. The center should employ, either on a part-time or full-time basis, a center director, nurses, doctors, chaplains, social workers, pharmacists, dieticians, therapists, and volunteers. In addition, it will need an administrative staff, facility managers and nurses aides. The hospice should also be equipped with facilities for patients, their families, and team members, furnished with equipment and goods at the same level of a hospital. represented by a center director who reports to a board and an advisory committee. Also, the center director administers a steering committee and five departments, namely, Administration, Nursing Service, Social Welfare, Religious Services, and Medical Service. Furthermore, the center should be able to utilize a direct and support delivery systems. The direct delivery system allows the hospice center to receive requests from, or transfer patients to, hospitals, clinics, other hospice organizations (by type), public health centers, religious organizations, social welfare organizations, patients, and their guardians. On the other hand, the support delivery system provides a link to outside facilities of various medical suppliers. In terms of management, details were made with regards to personnel management, records, infection control, safety, supplies and quality management. For financial support, some form of medical insurance coverage for hospice services, ways to promote a donation system and fund raising were examined. Hospice team service to be provided by the hospice center was categorized into assessment, physical care, emotional care, spiritual care, bereavement service, medication, education and demonstrations, medical supplies rental, request service, volunteer service, and respite service. Based on the results, the study has drawn up the following suggestions: 1. The proposed model for a hospice center as presented in the study needs to be tested with a pilot project. 2. Studies on criteria for legal approval and license for a hospice center need to be conducted to develop policies. 3. Studies on developing a hospice charge system and hospice standards that meet local conditions in Korea need to be conducted.

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아동·청소년 관련 공공 서비스 이용 분석: 학교급별 차이 (Analysis of Public Service Utilization for Children and Youth : comparison among different age groups)

  • 장연진;서정아
    • 한국콘텐츠학회논문지
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    • 제20권6호
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    • pp.653-667
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    • 2020
  • 본 연구는 아동·청소년 관련 시설 및 기관을 이용하는 초, 중, 고 학생들의 이용 실태와 중복 이용 경험을 분석하여, 효과적인 아동·청소년 서비스 전달체계를 제안하기 위한 목적을 지니고 있다. 이를 위해, 아동과 청소년이 이용하는 공공 서비스 전달체계를 중심으로 초, 중, 고 학교급별 차이를 통계 분석과 네트워크 분석방법을 활용하여 분석하였다. 주요 연구결과에 따르면, 일반 시설(기관) 이용율에서 학교급별 차이가 나타난 곳은 주민자치센터, 청소년수련관(수련원), 건강가정지원센터, 보건소, 시청·도청, 군청·구청, 교육청이었다. 복지 시설(기관) 중에서는 위(Wee)센터, 위(Wee) 스쿨, 지역아동센터에서 학교급별 차이가 나타났다. 네트워크 연결 중심성 분석에서는 초등학생은 보건소, 중학생은 청소년 수련관, 고등학생은 주민자치센터가 가장 높게 나타나, 자체 및 중복 이용자 수가 가장 높은 것으로 조사되었다. Concor를 이용한 구획화 결과에서도 동일한 시설(기관)이라도 학교급에 따라 서로 다른 기관 간 연계가 이루어지고 있는 것으로 나타났다. 이러한 연구결과를 토대로 향후 아동·청소년 서비스 전달체계의 질적 개선을 위해서, 생애주기적 특성을 고려한 통합 게이트웨이 구축과 개인별 시설(기관) 이용 데이터베이스 개발을 제안하였다.

요양보호사의 기관 및 서비스 평가를 통한 재가노인서비스 품질 향상 방안 (A Study on the Improvement of In-Home Care Service Quality through Evaluation of Services and Agency by Long-term Care Workers)

  • 배화숙;한정원
    • 디지털융복합연구
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    • 제14권10호
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    • pp.71-81
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    • 2016
  • 본 연구의 목적은 서비스 공급자로서 재가요양서비스를 제공하고 있는 요양보호사의 서비스에 대한 평가를 통해 서비스 품질 개선 방안을 찾는 것이다. 이를 위해 부산에서 활동 중인 요양보호사 223명을 대상으로 직무 환경, 서비스 기관에 대한 평가, 재교육 등에 대한 내용을 중심으로 설문조사를 실시하였다. 조사결과 요양보호사는 학력은 낮지 않으나 연령대가 높고 불안정한 고용형태를 가진 이가 대다수였다. 더 나은 요양서비스를 위해 희망하는 슈퍼비전 내용은 서비스 이용자와의 관계성, 이용자 욕구 변화에 따른 대처 등이었다. 우선적으로 필요한 재교육 내용은 노년기 및 노인 가족에 대한 이해, 노인성 질환에 대한 보건 의료적 지식, 이용자 및 가족과의 상담기법 등으로 나타났다. 그리고 건강보험공단 등에 바라는 것은 이용자와 보호자의 인식 개선, 급여 인상, 그리고 전문성 강화 위한 교육 기회 확대 등이었다. 이상의 연구결과를 통해 요양보호서비스 향상을 위한 방안으로 서비스 공급구조적 측면에서의 개선과 요양보호사와 업무에 대한 인식개선, 적절한 슈퍼비전과 재교육 기회 제공 등을 제안하였다.

자활사업 참여자의 정서적 자활과 사회적 자활의 영향요인 연구: 사회적응프로그램 참여자를 중심으로 (Factors Affecting Emotional and Social Self-Sufficiency of Welfare-to-Work Recipients: Focused Social Adaptation Program)

  • 이은정
    • 디지털융복합연구
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    • 제12권2호
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    • pp.539-548
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    • 2014
  • 본 연구는 국민기초생활보장제도 자활사업의 탈수급 탈빈곤의 한계에도 불구하고 자활참여자의 복합적이고 다양한 근로장벽 특성상 자활 효과의 다차원적인 접근으로 정서적, 사회적 자활의 역할에 주목하고자 한다. 이를 위해 자활프로그램 중 심리사회적 어려움으로 가장 자활가능성이 취약한 사회적응프로그램 참여자의 정서적 자활과 사회적 자활의 효과 및 그 영향요인을 검증하고자 한다. 조사 대상은 전국의 사회적응프로그램 수행기관의 참여자를 대상으로 표집하였으며, 위계적 회귀분석(Hierarchical regression)이 검증에 활용되었다. 분석결과, 첫째, 사회적응프로그램 참여자의 정서적 자활 효과와 사회적 자활 효과는 보통 수준을 상회하는 것으로 나타났다. 둘째, 정서적 자활의 영향요인으로는 요보호가구원수(-)와 교육프로그램 만족도(+)가 유의미한 영향력이 있음이 밝혀졌고 사회적 자활에는 삶의 만족도(+), 조직유형, 교육프로그램 만족도(+), 건강서비스 만족도(+)가 긍정적 영향을 미치는 것으로 나타났다. 결론적으로 자활사업의 평가와 관련된 정서적, 사회적 측면의 자활효과 측정의 필요함을 밝히고 심리사회적 다양한 근로장벽을 지닌 자활참여자 특성에 적합한 통합사례관리 강화, 프로그램 개발 및 서비스 지원체계 등에 관한 정책적, 실천적 함의를 제시하였다.

Angiostrongylus cantonensis: Scanning Electron Microscopic Observations on The Cuticle of Moulting Larvae

  • Zeng, Xin;Wei, Jie;Wang, Juan;Wu, Feng;Fung, Feng;Wu, Xiaoying;Sun, Xi;Zheng, Huanqing;Lv, Zhiyue;Wu, Zhongdao
    • Parasites, Hosts and Diseases
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    • 제51권6호
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    • pp.633-636
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    • 2013
  • Angiostrongylus cantonensis is a parasitic nematode that needs to develop in different hosts in different larval stages. Freshwater snails, such as Pomacea canaliculata, are the intermediate host, and rats are the definitive host. Periodic shedding of the cuticle (moulting) is an important biological process for the survival and development of the parasite in the intermediate and definitive hosts. However, there are few studies on the cuticle alterations between different stages of this parasite. In this study, we observed the ultrastructural appearance and changes of the cuticle of the 2nd/3rd stage larvae (L2/L3) and the 3rd/4th stage larvae (L3/L4) using a scanning electron microscope. We also first divided L2/L3 into late L2 and early L3. The late L2 lacked alae, but possessed a pull-chain-like fissure. Irregular alignment of spherical particles on the cuticle were noted compared to the L3. Alae appeared in the early L3. The old cuticle turned into a thin filmlike structure which adhered to the new cuticle, and spherical particles were seen regularly arranged on the surface of this structure. Regular rectangular cavities were found on the surface of L3/L4. The caudal structure of L3/L4 was much larger than that of L3, but caudal inflation, such as seen in L4, was not observed. These results are the first to reveal the ultrastructural changes of the cuticle of A. cantonensis before and after moulting of L2/L3 and L3/L4.