• Journal of Forest and Environmental Science
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• 제16권1호
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• pp.119-123
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• 2000

본 연구는 GIS를 이용하여 산림경영을 위한 의사결정을 지원할 수 있는 방안을 연구하였다. 그리고 임목의 벌채로 인한 수질오염문제, 토사유출문제를 해결하기 위하여 우선 입목을 벌채를 실시할 경우, 별채 금지구역을 선정하였다. 강원대학교 연습림 지역에 대한 벌채계획 수립 시, 벌채로 인한 여러 가지 위험요소들이 발생하므로 벌채제한지의 선정은 매우 중요한 일이다. 따라서 본 연구에서는 이용된 GIS 분석방법으로는 Arc/Info 7.1의 기능 중에 하나인 버퍼링 (Buffering) 기능과 중첩기능 (Overlay)를 이용하여 분석을 실시하였다. 연구결과, 전체 249.82ha에서 52.64ha가 벌채제한지로 나타났고, 수종별로 살펴보면 활엽수가 13.21ha, 잣나무 26.44ha, 낙엽송 12.38ha, 소나무 0.61ha 이었다. 잣나무가 26.44ha로 가장 많은 지역에서 벌채제한지로 나타났다.

• 현장농수산연구지
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• 제18권1호
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• pp.13-24
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• 2016

본 연구는 비타민 A와 D의 공급제한이 거세 한우의 육질등급에 미치는 영향을 조사하기 위해 수행하였다. 비육전기(15개월령) 거세 한우 60두를 총 4개의 처리구[대조구(C), 비타민 A 제한구(-A), 비타민 D 제한구(-D)와 비타민 A 및 D 제한구(-AD)]에 15두씩 배치하여, 15개월령부터 23개월령까지 총 8개월 동안 처리구에 따라 비타민 A 및 D를 첨가하지 않는 방법으로 비타민 공급을 제한하였다. 이후 24개월령부터 26개월령까지는 비타민 A 및 D를 사료 원물의 0.05% 첨가, 27개월령부터 출하까지는 0.1% 첨가하여 비타민 A 및 D 공급이 제한되지 않도록 하였다. 본 연구의 결과를 종합하면, 15개월령 거세 한우에게 비타민 A와 D의 공급을 8개월동안 제한시킨 결과 1일 건물 섭취량에는 영향을 미치지 않았고 또한 체중, 일당증체량 및 사료 요구율에도 영향을 미치지 않았다. 그러나 혈중 retinol 농도는 비타민 A의 공급 제한에 의해 최대 약 50% 정도까지 감소하였다. 또한 혈중 25(OH)D3 농도는 비타민 D의 공급제한에 의하여 최대 약 55% 정도까지 감소하였다. 거세 한우의 비타민 A 및 D 공급 제한은 도체중, 등지방두께, 배최장근 단면적, 육량지수 및 육량등급에 영항을 미치지 않은 것으로 나타났다. 반면 비타민 A의 공급 제한은 마블링지수를 17% 정도 유의적으로 향상시켰다. 비타민 D의 공급 제한은 마블링 지수를 유의적으로 증가시키지는 않았지만 수치적으로(약 5%) 증가시키는 경향을 보였다. 결론적으로 본 연구결과는 거세 한우의 근내지방 침착 향상을 위해서는 비타민 A의 공급제한 방법이 비타민 D 공급제한 보다 효율적임을 제시하였고 한우에서도 일본의 화우와 마찬가지로 비육전기에 비타민 A를 제한 할 경우 근내지방도 향상을 통해 고급육생산이 가능함을 제시하였다.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2005년도 총회 및 춘계학술발표회
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• pp.28-30
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• 2005

Because of high population diversity in soil microbial communities, it is difficult to accurately assess the capability of biodegradation of toxicant by microbes in soil and sediment. Identifying biodegradative microorganisms is an important step in designing and analyzing soil bioremediation. To remove non-important noise information, it is necessary to selectively enrich genomes of biodegradative microorganisms fromnon-biodegradative populations. For this purpose, a stable isotope probing (SIP) technique was applied in selectively harvesting the genomes of biphenyl-utilizing bacteria from soil microbial communities. Since many biphenyl-using microorganisms are responsible for aerobic PCB degradation In soil and sediments, biphenyl-utilizing bacteria were chosen as the target organisms. In soil microcosms, 13C-biphenyl was added as a selective carbon source for biphenyl users, According to $13C-CO_2$ analysis by GC-MS, 13C-biphenyl mineralization was detected after a 7-day of incubation. The heavy portion of DNA(13C-DNA) was separated from the light portion of DNA (12C-DNA) using equilibrium density gradient ultracentrifuge. Bacterial community structure in the 13C-DNAsample was analyzed by t-RFLP (terminal restriction fragment length polymorphism) method. The t-RFLP result demonstates that the use of SIP efficiently and selectively enriched the genomes of biphenyl degrading bacteria from non-degradative microbes. Furthermore, the bacterial diversity of biphenyl degrading populations was small enough for environmental genomes tools (metagenomics and DNA microarrays) to be used to detect functional (biphenyl degradation) genes from soil microbial communities, which may provide a significant progress in assessing microbial capability of PCB bioremediation in soil and groundwater.

• Archives of Plastic Surgery
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• 제47권2호
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• pp.194-197
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• 2020

Anterior neck burns represent a major reconstructive challenge due to severe sequalae including restriction in movement and poor aesthetic outcomes. Common treatment options include skin grafting with/without dermal matrices, and loco-regional and distant free flap transfers with/without prior tissue expansion. Such variation in technique is largely influenced by the extent of burn injury requiring resurfacing. In order to optimize like-for-like reconstruction of the anterior neck, use of wide, thin and long flaps such as the anterolateral thigh (ALT) perforator flap have been reported with promising results. Of note, some patients have a tendency towards severe scar contractures, which may be contributed by the greater extent of inflammation during wound healing. We report our experience at 4 years' followup after secondary reconstruction of severe, anterior neck burn contractures in two patients by harvesting the ALT flap with a butterfly design. This technique provides adequate wound resurfacing of the burned neck and surrounding areas, and provides good neck extensibility by addressing both anterior and lateral aspects of the scar defect simultaneously. Such a flap design reduces tension on wound edges and thus, the risk of contracture recurrence in what remains a particularly challenging type of burn reconstruction.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제28권5호
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• pp.375-395
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• 2006

Purpose: Lingual nerve (LN) damage may be caused by either tumor resection or injury such as wisdom tooth extraction, Although autologous nerve graft is sometimes used to repair the damaged nerve, it has the disadvantage of necessity of another operation for nerve harvesting. Moreover, the results of nerve grafting is not satisfactory. The nerve growth factor (NGF) is well-known to play a critical role in peripheral nerve regeneration and its local delivery to the injured nerve has been continuously tried to enhance nerve regeneration. However, its application has limitations like repeated administration due to short half life of 30 minutes and an in vivo delivery model must allow for direct and local delivery. The aim of this study was to construct a well-functioning $rhNGF-{\beta}$ adenovirus for the ultimate development of improved method to promote peripheral nerve regeneration with enhanced and extended secretion of hNGF from the injured nerve by injecting $rhNGF-{\beta}$ gene directly into crush-injured LN in rat model. Materials and Methods: $hNGF-{\beta}$ gene was prepared from fetal brain cDNA library and cloned into E1/E3 deleted adenoviral vector which contains green fluorescence protein (GFP) gene as a reporter. After large scale production and purification of $rhNGF-{\beta}$ adenovirus, transfection efficiency and its expression at various cells (primary cultured Schwann cells, HEK293 cells, Schwann cell lines, NIH3T3 and CRH cells) were evaluated by fluorescent microscopy, RT-PCR, ELISA, immunocytochemistry. Furthermore, the function of rhNGF-beta, which was secreted from various cells infected with $rhNGF-{\beta}$ adenovirus, was evaluated using neuritogenesis of PC-12 cells. For in vivo evaluation of efficacy of $rhNGF-{\beta}$ adenovirus, the LNs of 8-week old rats were exposed and crush-injured with a small hemostat for 10 seconds. After the injury, $rhNGF-{\beta}$ adenovirus($2{\mu}l,\;1.5{\times}10^{11}pfu$) or saline was administered into the crushed site in the experimental (n=24) and the control group (n=24), respectively. Sham operation of another group of rats (n=9) was performed without administration of either saline or adenovirus. The taste recovery and the change of fungiform papilla were studied at 1, 2, 3 and 4 weeks. Each of the 6 animals was tested with different solutions (0.1M NaCl, 0.1M sucrose, 0.01M QHCl, or 0.01M HCl) by two-bottle test paradigm and the number of papilla was counted using SEM picture of tongue dorsum. LN was explored at the same interval as taste study and evaluated electro-physiologically (peak voltage and nerve conduction velocity) and histomorphometrically (axon count, myelin thickness). Results: The recombinant adenovirus vector carrying $rhNGF-{\beta}$ was constructed and confirmed by restriction endonuclease analysis and DNA sequence analysis. GFP expression was observed in 90% of $rhNGF-{\beta}$ adenovirus infected cells compared with uninfected cells. Total mRNA isolated from $rhNGF-{\beta}$ adenovirus infected cells showed strong RT-PCR band, however uninfected or LacZ recombinant adenovirus infected cells did not. NGF quantification by ELISA showed a maximal release of $18865.4{\pm}310.9pg/ml$ NGF at the 4th day and stably continued till 14 days by $rhNGF-{\beta}$ adenovirus infected Schwann cells. PC-12 cells exposed to media with $rhNGF-{\beta}$ adenovirus infected Schwann cell revealed at the same level of neurite-extension as the commercial NGF did. $rhNGF-{\beta}$ adenovirus injected experimental groups in comparison to the control group exhibited different taste preference ratio. Salty, sweet and sour taste preference ratio were significantly different after 2 weeks from the beginning of the experiment, which were similar to the sham group, but not to the control group.