• Korean Journal of Environmental Biology
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• v.40 no.4
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• pp.363-373
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• 2022

On April 29, 2021 (1st), June 2 (2nd), and August 17 (3rd), we surveyed benthic macroinvertebrates fauna at Muljangori-oreum wetland in Bonggae-dong, Jeju Island, Korea. Muljangori-oreum wetland was divided into four areas. The survey was conducted in three accessible areas (areas 1-3). As a result of habitat environment analysis, the average monthly temperature from 2017 to 2021 was the highest in July and August and the lowest in December and February. This pattern was repeated. As a result of analyzing changes in vegetation and water surface area through satellite images, normalized difference vegetation index (NDVI) increased from February to July and decreased after July. Normalized difference water index (NDWI) was analyzed to show an inverse relationship. A total of 21 species from 13 families were identified in the qualitative survey and a total of 412 individuals of 24 species from 15 families were identified in the quantitative survey. A total of 26 species from 17 families, 8 orders, 3 classes, and 2 phyla of benthic macroinvertebrates were identified. The dominant species was Chronomidae spp. with 132 individuals (32.04%). Noterus japonicus was a subdominant species with 71 individuals (17.23%). As a result of comparative analysis of species identified in this study and the literature, it was confirmed that species diversity was high for Coleoptera and Odonata. Main functional feeding groups (FFGs) were found to be predators. Habitat orientation groups (HOGs) were found to be swimmers. In OHC (Odonata, Hemiptera, and Coleoptera) group, 17 species (73.91%) in 2021, 23 species (79.31%) in 2016, 26 species (86.67%) in 2018, and 19 species (79.17%) in 2019 were identified. Cybister japonicus, an endangered species II, was confirmed to inhabit Muljangori-oreum wetland in the literature. Ten individuals (2.43%) were also confirmed to inhabit Muljangori-oreum wetland in 2021. Therefore, continuous management and habitat protection are required to maintain the habitat environment of C. japonicus in Muljangori-oreum wetland.

• Knowledge Management Research
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• v.23 no.4
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• pp.91-112
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• 2022

In order to improve the quality of consumption in senior generation, it is necessary to build trust in the digital consumption environment. However, there are limitations from previous researches on consumption in the digital environment that has mainly focused on Millennial and Z generations. Therefore, this study aims to examine the antecedents of digital consumption trust and to explore the market segments of senior generation created by the dynamics of these antecedents. In addition, in order to provide practical implications, we investigate the difference in the level of perceived digital consumption trust using segmentation. This study, therefore, used 2021 Korea's consumer life index data conducted by the Korea Consumer Agency for general consumers, and only consumer data of 60s and older are extracted for the analysis. As a result, we confirm from the main results that the three antecedents, consumer competency, perceived corporate responsibility, and local community's problem-solving environment, are significant in building the digital consumption trust in the senior generation. It is academically significant in this aspect to look at approaches to improve senior customers' trust in digital consumption circumstances by dividing generations because generations exhibit varying levels of understanding of online consumption or digital consumption conditions. It is academically significant in this aspect to look at approaches to improve senior customers' trust in digital consumption circumstances by dividing generations because generations exhibit varying levels of understanding of online consumption or digital consumption conditions. Additionally, it is proposed as a practical implication that it should be managed so that significant improvements of customers, businesses, and regional public institutions are developed in order to allow the senior consumers to prepare trusted digital consumption circumstances.

• Journal of Korean Society of Forest Science
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• v.111 no.1
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• pp.136-149
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• 2022

In this study, the performance of a nonlinear mixed-effects (NLME) model used to estimate the stem taper of Pinus densiflora in Gangwon Province was compared with that of a nonlinear fixed-effects (NLFE) model using several performance measures. For the diameters of whole tree stems, the NLME model improved on the performance of the NLFE model by 26.4%, 42.9%, 43.1%, and 0.9% in terms of BIAS, MAB, RMSE, and FI, respectively. For the cross-section areas of whole tree stems, the NLME model improved on the performance of the NLFE model by 67.7%, 44.7%, 45.8%, and 1.0% in terms of BIAS, MAB, RMSE, and FI, respectively. Based on the analysis of 12 relative height classes of tree stems, stem taper estimation performance was also reasonably improved by the NLME model, which showed better MAB, RMSE, and FI at every relative height class compared with those of the NLFE model. In some classes, the NLFE model had better BIAS than the NLME model (stem diameter: 0.05, 0.2, 0.3, and 0.8; stem cross-section area: 0.05, 0.3, 0.5, 0.6, and 1.0). However, the NLME model enhanced the performance of stem diameter and cross-section area estimations at the lowest stem part (0.2 m from the ground). Improvements for stem diameter in terms of BIAS, MAB, RMSE, and FI were 84.2%, 69.8%, 68.7%, and 3.1%, respectively. For stem cross-section areas, the improvements in BIAS, MAB, RMSE, and FI were 98.5%, 70.1%, 68.7%, and 3.1%, respectively. The cross-section area at 0.2 m from the ground occupied 22.7% of total cross-section area. Improvements in estimation of cross-section area at the lowest stem part indicate that stem volume estimation performance could also be enhanced. Although NLME models are more difficult to fit than NLFE models, the use of NLME models as a standard method for the estimating the parameters of stem taper equations should be considered.

• Journal of Korean Society of Forest Science
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• v.111 no.1
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• pp.115-124
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• 2022

This study was conducted to obtain basic data that could help prevent damage caused by slow-moving landslides (land-creep). Specifically, the geological, topographic, and physical characteristics of land-creep were analyzed in Jiphyeon-myeon, Jinju-si. The first and second analyzed land-creeps occurred in 1982 and 2019, respectively. The area damaged in the second land-creep was about 11.5-fold larger than that damaged in the first land-creep. The dominant constituent rock in the land-creep area was sedimentary rock, which seems to be weakly resistant to weathering. The areas that collapsed due to land-creep were related to the presence of separated rocks between the bedding plane in the estimated activity surface over the slope direction and the vertically developed joint surface. Thus, surface water and soil debris were introduced through the gaps of separated rocks. Additionally, the areas collapsed due to the combination of the bedding plane and joint surface shale and sandstone showed an onion structure of weathered outcrop from the edge to inner part caused by weathering from ground water. Consequently, core stones were formed. The study area was a typical area of land-creep in a mountain caused by ground water. Land-creep was classified into convex areas of colluvial land-creep. The landslide-risk rating in the study area was classified into three and five classes. The flow of ground water moved to the northeast and coincided with the direction of the collapse. Soil bulk density in the collapsed area was lower than that in ridge area, which was rarely affected by land-creep. Thus, soil bulk density was affected by the soil disturbance in the collapsed area.

• The Journal of the Convergence on Culture Technology
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• v.8 no.3
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• pp.427-434
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• 2022

This study aims to develop design elements for cultural convergence between rehabilitation for individuals with developmental disabilities and lifelong education for individuals with disabilities, which is a key area in the practical support system for independent life support for individuals with developmental disabilities. As for the research method, a procedure for conducting FGI by forming two teams for professors majoring in special education and rehabilitation science was formed. The research was presented in three upper categories (universal cultural convergence elements, field-centered cultural convergence elements, and policy-centered cultural convergence elements) that should be designed for cultural convergence between rehabilitation for individuals with developmental disabilities and lifelong education for individuals with disabilities. In addition, subcategories were specifically composed for each upper category. First, as a universal cultural element, "open creative convergence" was presented in principle, which can be explained as a principle of exploring and practicing the validity of convergence between related fields for rehabilitation for individuals with developmental disabilities and lifelong education for individuals with disabilities. Second, field-centered cultural factors included development of joint practice model between fields of rehabilitation science and special education, subject matter education knowledge and skills, teaching and learning methods, learning career roadmaps, employment and job career development roadmaps, and the formation of an independent life development history certification system. Third, as policy-centered cultural elements, the formation of a curriculum integration composition system between local related institutions, the establishment of a qualification development path for coordinator-professional teacher-type personnel, and the organizational systematization between school-center types were presented. The study concluded that independent life support for individuals with developmental disabilities should not only be guaranteed for the entire life of adulthood, but also a lifelong education for individuals with disabilities based rehabilitation support system for individuals with developmental disabilities should be established through cultural convergence.

• Biomolecules & Therapeutics
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• v.30 no.2
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• pp.203-211
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• 2022

Melanogenesis is the production of melanin from tyrosine by a series of enzyme-catalyzed reactions, in which tyrosinase and DOPA oxidase play key roles. The melanin content in the skin determines skin pigmentation. Abnormalities in skin pigmentation lead to various skin pigmentation disorders. Recent research has shown that the expression of EMP2 is much lower in melanoma than in normal melanocytes, but its role in melanogenesis has not yet been elucidated. Therefore, we investigated the role of EMP2 in the melanogenesis of MNT1 human melanoma cells. We examined TRP-1, TRP-2, and TYR expression levels during melanogenesis in MNT1 melanoma cells by gene silencing of EMP2. Western blot and RT-PCR results confirmed that the expression levels of TYR and TRP-2 were decreased when EMP2 expression was knocked down by EMP2 siRNA in MNT1 cells, and these changes were reversed when EMP2 was overexpressed. We verified the EMP2 gene was knocked out of the cell line (EMP2 CRISPR/Cas9) by using a CRISPR/Cas9 system and found that the expression levels of TRP-2 and TYR were significantly lower in the EMP2 CRISPR/Cas9 cell lines. Loss of EMP2 also reduced migration and invasion of MNT1 melanoma cells. In addition, the melanosome transfer from the melanocytes to keratinocytes in the EMP2 KO cells cocultured with keratinocytes was reduced compared to the cells in the control coculture group. In conclusion, these results suggest that EMP2 is involved in melanogenesis via the regulation of TRP-2 expression.

• Korean Journal of Animal Reproduction
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• v.24 no.4
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• pp.407-417
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• 2000

The present study was to examine effects of various electrical stimulus treatments used for electro-fusion on the preimplantation development of bovine nuclear transfer (NT) embryos with fetal fibroblast cells. Fetal fibroblast cells were isolated from one fetus at day 45 of gestation in Holstein cow, and passaged 3 to 4 times before being transferred into enucleated oocytes. Single fibroblast cells were individually placed into the perivitelline space of enucleated oocytes by using a micromanipulator. At first, the fusion and developmental rates of reconstructed oocytes were compared between different electric stimulation conditions. When fusion of the reconstructed oocyte was induced by different electric pulse periods (15, 30 and 45 $\mu$sec) at a DC pulse of 1.8 kV/cm, 15 (45.5%, 120/264) or 30 $\mu$ sec group (43.9%, 106/241) showed a higher fusion rate than 45 $\mu$sec group (23.2%, 58/250, P＜0.05). However, no difference was detected in the development rate of the fused oocytes to blastocysts between groups. Next experiment was to examine the effects of different electrical field strengths (1.5, 1.8 and 2.1 kV/cm) for 15 $\mu$sec at electrofusion on in vitro development of the NT embryos. As results, there was no difference in the fusion and developmental rates of the NT embryos between electrical strength (P＞0.05). Finally, developmental competence of bovine NT embryos with somatic cells was compared with IVF-derived embryos. Of enucleated oocytes fused with fibroblast cells, 27.4% (75/274) developed to the blastocyst stage, which is similar to that (24.5%, 58/237) of IVF-derived embryos. However, mean nuclei number of NT blastocysts was smaller than that of IVF-derived blastocysts. Thus, we have established an optimal condition (1.8 kV/cm, 15 $\mu$sec) for electric fusion of bovine NT oocytes with somatic cells. The present study indicates that bovine reconstructed embryos with somatic cells normally develop to blastocyst stage in vitro, although having smaller nuclei numbers of blastocysts as compared to IVF-derived embryos.

• Korean Journal of Plant Taxonomy
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• v.52 no.1
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• pp.54-63
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• 2022

Prunus subg. Cerasus is the most planted street and landscaping tree in South Korea, but it is difficult to identify species according to their macro-morphologies, leading to problems when attempting properly to manage species quantities. The purpose of this study is to understand the current status of plant types and species compositions in Bundang Central Park in Bundang-gu of Seongnam City and to discuss the necessity of the management of landscaping tree planting. In April of 2021, during the cherry blossom season, a total of 5,866 planted cherry trees were investigated within an area of 6 km² of Bundang-gu in Seongnam City. As a result, 5,744 trees were sorted into eleven taxa, but the remaining 122 trees were not determined due to their complex morphologies. Prunus ×yedoensis Matsum. accounted for the highest proportion (52.1%), followed by P. serrulata Lindl. var. pubescens Nakai and P. jamasakura Siebold ex Koidz. P. ×nudiflora (Koehne) Koidz., a plant native to Jejudo Island, was not found in this survey. In order to help identify cherry trees based on micro-morphologies, an identification key was presented for the eleven taxa planted as major landscaping trees. It is known that cherry trees frequently form interspecific hybrids in nature. In order to prevent a loss of the genetic originality of native species due to hybridization and gene introgression from foreign cherry trees, it is necessary to manage planting species near the habitats of native taxa and track their origins.

• Laboratory Medicine Online
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• v.2 no.1
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• pp.10-14
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• 2012

Background: Measurement of HbA_1c levels is widely used to diagnose diabetes mellitus and to evaluate and monitor plasma-glucose concentrations over 6-8 weeks. In this study, we evaluated the diagnostic performance of the newly developed latex immunoturbidimetric method by using Autolab HbA_1c. Methods: We analyzed and compared the diagnostic performance of Autolab HbA_1c with that of Toshiba 200FR between April 2009 and July 2009. According to guidelines (EP5-A2, EP6-P, EP9-A2) of the clinical and laboratory standards institute (CLSI), we compared linearity, precision and correlation of Autolab HbA_1c with those of G7 (Tosoh Corp., Kyoto, Japan) by using high-performance liquid chromatography (HPLC) method. Results: Data obtained using Autolab HbA_1c showed good linearity in mixtures of samples with low (3.1%) and high (15.1%) levels of HbA_1c (r²=0.9997). In the analysis of within-run precision of the samples with HbA_1c levels of 5.1% and 12.1%, the SDs were 0.04 and 0.06 and covariances of these samples were 0.8% and 0.5%, respectively. In the Deming regression model, the regression equation was as follows: Autolab HbA_1c=1.0859×Tosoh HPLC-0.6957. Conclusions: In this study, Autolab HbA_1c method showed better performance characteristics than Tosoh G7 did. In reference review, there was no interference of variant hemoglobin. The data acquisition time of Autolab HbA_1c was lower than that of Tosoh G7. The advantages of Autolab HbA_1c are that it can be used as an autoanlyzer in routine chemical analysis, it does not require pre-analytical treatment, and the samples are automatically treated with distilled water for hemolysis.

• Journal of Popular Narrative
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• v.25 no.4
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• pp.429-465
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• 2019

This paper analyzed the ways in which "others" were reproduced in Korean movies in the 1970s. In the midst of the social changes of the era, such as urbanization due to rapid industrial modernization, many people became laborers for industry in order to obtain the fruits of modernization.But the landscape of others, which was inevitably produced in the process of constructing such subjects, has been limited to analysis that is focused on gender and youth discourse. This article aims to extract the landscape of others in the 1970s by adopting a different perspective. The way in which the other is present can be divided into the following two categories. First, in 1970s film, the family community, in contrast with 1960s film, has disintegrated and cracked, due to the inability of others to enter or leave the community. The desperate perception that the family community can no longer function as a stable foundation or center of the constitution, and that it cannot have a sense of security and belonging,is revealed through the way the others are wandering in and out of the community. Second, 'Death' is an element of social life in the violence of the national ideology of the 1970s, and the everyday exceptional state. The way in which the 'other' is completely eliminated from the normal subjectivity requested by the state and is deported in film reflectshow everyday death or potential death is part of life of the 1970s. Normal life pursued through rapid urbanization and industrialization leads to the death of the other beings, but the way of existence of others is the desperate reality of the 1970s, when the boundaries of the state that provide stability and belonging are broken. As a result, the landscape of others in the 1970s reveals a violent reality that destroys the perfect middle class family discourse that industrial modernization was oriented around in the 1970s, and that produced masses of others who caused numerous deaths. In spite of regime censorship, Korean films were popularly revealing the violence of life brought in by the 1970s, following a detour of representation.