• Title/Summary/Keyword: hRA

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Application of Ultrasonic Vibration Energy on Eco-superfinishing and Surface Hardening Treatment of Cold Work Roller (초음파 진동에너지를 이용한 냉간 압연롤러 표면의 환경 친화적 초정밀 사상 및 표면 경화 처리 및 시험)

  • Y.S. Pyoun;Park, J.H.;C.H. Han;Park, Y.;I.S. Cho;N. Azuma;Lee, J.H.;Kim, C.S.;Park, C.H.
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2002.05a
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    • pp.39-39
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    • 2002
  • In order to improve wear and fatigue resistance of the structural materials, especially cold work roller for 304 stainless steel, an eco-super-finishing and surface hardening treatment using ultrasonic vibration energy was developed and applied to the SKD-ll roller. The eco-super-finishing machine was designed and fabricated by DesignMecha Co, by its own technology. It was observed that the surface roughness, hardness and residual stress were changed from $Ra{\;}={\;}O.25\mu\textrm{m}$, Hv=710 and ${\sigma}$={\;}+400{\;}MPa{\;}to{\;}Ra{\;}={\;}0.165\mu\textrm{m}$, Hv = 1200 and ${\sigma}=-610$ MPa after 20 KHz micro-cold forging, which means almost equal to the 300 % improvement of life-time.

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Conformation of Retinoic Acid and Structure-Activity Relationships -Retinobenzoic Acid- (레티노익 산의 형태와 구조-활성 관계 -레티노벤조익 산-)

  • Rhee, Jong-Dal;Rhee, In-Ja
    • YAKHAK HOEJI
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    • v.38 no.3
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    • pp.230-237
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    • 1994
  • The structure-activity relationships of (E)-chalcone-4-carboxylic acids, flavone-4'-carboxylic acids, two types of aromatic amides, terephthalic monoanilides, and (arylcarboxamido)benzoic acids, which were made by Shudo group, are discussed by conformation analysis(AM1) of retinoic acid and those compounds. Conformer of each compound is superimposed on the conformationally restricted compound, 4-(6,7,8,9-tetrahydro-6,6,9,9-tetramethyl-4H-4-oxonaphto[ 2,3-b]pyran-2-yl) benzoic acid(Fv80), possessing the strongest differentiation-inducing activity on human promyelocytic leukemia cells HL-60. The results indicated that the lengths between the carboxylic carbon and the two 6, 9 carbons binding to dimethyl, 1.20 nm and 1.09 nm, as well as the planarity of molecule are very important factors for the activity, especially 1.20 nm. In the case of the recently synthesized azulenic retinoic acids by Sato, et al. in 1993, the distance probably is also important, resulted from superimposing them on a Ch55 conformer and Fv80. The distance 1.0 nm is also important in Ch55. Several conformers of all-trans retinoic acid (RA) are well superimposed on the almost non-flexible Fv80, RA, 9-cis RA, and, specifically s-10,12 cis RA. And a simple hexangular model of RA is suggested to draw RA conformers easily without computer drawing model or molecular model.

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Comparison of DNA isolation methods for detection of foodborne pathogens by real-time PCR from foods (식품으로부터 식중독 세균 검출을 위한 Real-time PCR에 적합한 DNA 추출 방법 비교)

  • Koo, Eun-Jeong;Kim, Dongho;Oh, Se-Wook
    • Korean Journal of Food Science and Technology
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    • v.48 no.4
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    • pp.335-340
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    • 2016
  • This study was conducted to find out the most suitable DNA isolation methods for PCR detection of foodborne pathogens. Four DNA isolation methods including Universal Genomic DNA Extraction Kit (TaKaRa), PrepMan Ultra (Applied Biosystems), boiling method and alkaline lysis method (w/PEG) were tested and compared. The Universal Genomic DNA Extraction kit (TaKaRa) was considered as the more efficient isolation method for Escherichia coli O157:H7 and Staphylococcus aureus in lettuce, fish and beef. Meanwhile to detect the foodborne pathogens directly from foods without enrichment, the four different buffers such as double-distilled water, saline, glycine-saline, glycine-saline with Tween-20 and beef extract were also evaluated. As a result, saline was more suitable buffer for E. coli O157:H7. And double-distilled water was more suitable buffer than saline for S. aureus, respectively

Natural Convection Heat Transfer with a Rectangular Obstruction in a square Enclosure (직사각형 전도성 장애물을 갖는 밀폐공간내에서의 자연대류)

  • Choo, H.L.;Kim, B.H.;Kim, H.W.;Jang, C.S.
    • Solar Energy
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    • v.18 no.2
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    • pp.123-135
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    • 1998
  • The effect of the thermal conductivity of a centered, square, heat-conducting body on natural convection In a square enclosure was examined numerically. Numerical simulations was carried out for Pr=0.17, $Ra=1.0{\times}10^4,\;1.0{\times}10^5,\;1.0{\times}10^6$, $K^*$=1.0, 6.6, 34.0 and t=0.5, 1.0, 2.0. The results were reported in terms of streamlines, isotherms, Nusselt number. As the results, the mean Nusselt number increases with the increasing of ${\zeta}$ at a constant Ra and $K^*$. In the case of ${\zeta}=1.0$(obstruction shape ratio), the mean Nusselt numbers were decreased as increasing of $K^*$(obstruction thermal conductivity ratio) with regardless of the Rayleigh number. When the constant obstruction size and thermal conductivity ratio, convective heat transfer effect was more enhanced at ${\zeta}=2.0$ than ${\zeta}=0.5$.

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The Fermentation Characteristics of Newly Selected Thermotolerant Yeasts at High Temperature

  • Sohn, Ho-Yong;Park, Wan;Jin, Ing-Nyol;Seu, Jung-Hwn
    • Journal of Microbiology and Biotechnology
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    • v.4 no.3
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    • pp.222-229
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    • 1994
  • In order to develop a method of economical production and to reduce energy-consumption in fuel alcohol production, we investigated the fermentation characters of two newly selected thermotolerant yeasts. The RA-74-2 showed stable and superior fermentability between 30 and $40^{\circ}C$ in 20% glucose media in comparison to the industrial strains. The optimum concentration of glucose for economical fermentation at $40^{\circ}C$ was 15-18%, and organic nitrogen was necessary for a satisfactory fermentation. The optimum pH was 4.0 and aeration was adversed for high temperature fermentation. Agitation was an important factor at $40^{\circ}C$ and the addition of magnesium ion 0.2% was required in this experiment. When the inoculum was increased, ethanol productivity as well as the speed of fermentation increased. On the other hand RA-912, which can grow at $48^{\circ}C$, showed similar fermentability between 30-$45^{\circ}C$ in 20% glucose media As the concentration of substrate decreased, fermentation ratio increased at $45^{\circ}C$ (45%, 65%, 95% fermentation ratio in 20%, 15%, 10% glucose media, respectively). Also, requirement of organic nitrogen and magnesium ion in RA-912 was similar in RA-74-2. The optimum pH for fermentation was 5.0, and the effects of agitation were enhanced at $37^{\circ}C$ than at $45^{\circ}C$. As the inoculum was increased, fermentation speed became more enhanced but the ethanol productivity was less affected. RA-912 showed fermentability with various substrates. Among the substrates used, inulin was the most promising substrate for the high-temperature fermentation. When 14.5% inulin was used as the substrate, 93% and 55% fermentation ratios were shown at $37^{\circ}C$ and $45^{\circ}C$, respectively.

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A Study on the Corrosion Properties of Underwater Wet Arc Welds Using the SM 41 (선체용 압연 강판의 습식 수중 아크 용접부의 부식특성에 관한 연구)

  • Kwak, H.H.;Ki., C.G.;Kim, M.N.;Hwang, S.H.
    • Journal of Power System Engineering
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    • v.9 no.4
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    • pp.110-117
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    • 2005
  • Underwater wet arc welds were experimentally performed on 11mm thick KR-RA steel plate using six different types of flux coated electrodes of 4.0mm diameter, KSKR, KSKT, USBL, JPUW, UWEA and UWEB. From analysis of bead appearance, detachability of weld slag, spatter occurrence and arc stability, JPUW gives the best result, and UWEB is superior to KSKR and KSKT. By experimental result of hardness distribution on the weld bonds, UWEB weld has the narrowest bond structure which is probable condition to get the best mechanical properties of weld. UWEB and JPUW welds have more even hardness distribution across weld deposit and base metal. Upon polarization test to measure the respondency of corrosion, the electrode of UWEB shows the most excellent degree due to the low open circuit potential difference.

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A Study on the Corrosion Properties of Underwater Wet Arc Welds using the SM 41 (선체용 압연 강판의 습식 수중 아크 용접부의 부식특성에 관한 연구)

  • Kwak, H.H.;Kim, C.G.;Kim, M.N.;Hwang, S.H.
    • Journal of Power System Engineering
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    • v.10 no.4
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    • pp.111-118
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    • 2006
  • Underwater wet arc welds were experimentally performed on 11mm thick KR-RA steel plate using six different types of flux coated electrodes of 4.0mm diameter, KSKR, KSKT, USBL, JPUW, UWEA and UWEB. From analysis of bead appearance, detachability of weld slag, spatter occurrence and arc stability, JPUW gives the best result, and UWEB is superior to KSKR and KSKT. By experimental result of hardness distribution on the weld bonds, UWEB weld has the narrowest bond structure which is probable condition to get the best mechanical properties of weld. UWEB and JPUW welds have more even hardness distribution across weld deposit and base metal. Upon polarization test to measure the respondency of corrosion, the electrode of UWEB shows the most excellent degree due to the low open circuit potential difference.

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In vitro micropropagation of radish (Raphanus sativus L.) using callus induction and plant regeneration (캘러스 유기와 식물체 재분화를 이용한 무의 기내 대량증식)

  • You Kyoung Kim;Sug Youn Mo;Su Bin Choi;Han Yong Park
    • Journal of Plant Biotechnology
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    • v.50
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    • pp.155-162
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    • 2023
  • Radish (Raphanus sativus L.), a root vegetable grown worldwide, is consumed in several ways. In the cross between parental lines to produce F1 seeds of radish, the problem of low purity may arise because of pollen contamination. Therefore, we aimed to establish conditions for callus induction and regeneration so that in vitro cultured plants could be used for the propagation of stock seeds. The most effective hormone combination containing various concentrations of 2,4-D, TDZ, and kinetin was selected for callus induction using radish hypocotyl, and the induced calli were transferred to two types of hormone media to investigate the optimal conditions for shoot regeneration of the callus. The combination of 1 mg/L 2,4-D + 0.05 mg/L kin was the most effective for callus induction of RA2 and RA10, 1 mg/L 2,4-D + 0.1 mg/L kin + 0.025 mg/L TDZ of RA4, and 1 mg/L 2,4-D + 0.2 mg/L kin of RA30. Shoot regeneration of the RA4 callus occurred in both shoot regeneration media, but the frequency was much higher in the 5H+1B medium (1 mg/L NAA + 0.1 mg/L 2,4-D + 1 mg/L IPA + 0.02 mg/L GA3 + 2 mg/L zeatin + 1 mg/L BA). For the in vitro micropropagation of radish, the conditions selected in this study can assist in the propagation and maintenance of stock seeds to produce F1 seeds.