• Biomolecules & Therapeutics
• /
• v.23 no.5
• /
• pp.434-441
• /
• 2015

Histone deacetylase (HDAC) inhibitors are considered novel agents for cancer chemotherapy. We previously investigated MHY219, a new HDAC inhibitor, and its potent anticancer activity in human prostate cancer cells. In the present study, we evaluated MHY219 molecular mechanisms involved in the regulation of prostate cancer cell migration. Similar to suberanilohydroxamic acid (SAHA), MHY219 inhibited HDAC1 enzyme activity in a dose-dependent manner. MHY219 cytotoxicity was higher in LNCaP ($IC_{50}=0.67{\mu}M$) than in DU145 cells ($IC_{50}=1.10{\mu}M$) and PC3 cells ($IC_{50}=5.60{\mu}M$) after 48 h of treatment. MHY219 significantly inhibited the HDAC1 protein levels in LNCaP and DU145 cells at high concentrations. However, inhibitory effects of MHY219 on HDAC proteins levels varied based on the cell type. MHY219 significantly inhibited LNCaP and DU145 cells migration by down-regulation of matrix metalloprotease-1 (MMP-1) and MMP-2 and induction of tissue inhibitor of metalloproteinases-1 (TIMP-1). These results suggest that MHY219 may potentially be used as an anticancer agent to block cancer cell migration through the repression of MMP-1 and MMP-2, which is related to the reduction of HDAC1.

• Journal of Biosystems Engineering
• /
• v.28 no.1
• /
• pp.53-58
• /
• 2003

This study was carried out to design, construct, and test a greenhouse monitoring system fur the environment and status of control devices in a greenhouse from a remote site using internet. The measuring items selected out of many environmental factors were temperature, humidity, solar radiation, CO$_2$, SOx, NOx concentration, EC, pH of nutrient solution, the state of control devices, and the image of greenhouse. The developed greenhouse monitoring system was composed of the network system and the measuring module. The network system consists of the three kinds of monitors named the Croup Monitor. the Client Monitor and the Server Monitor. The results of the study are summarized as follows. 1. The measuring module named the House Monitor. which is used to watch the state of the control device and the environment of the greenhouse, was developed to a embedded monitoring module using one chip microprocessor 2. For all measuring items. the House Monitor showed a satisfactory accuracy within the range of ${\pm}$0.3%FS. The House Monitors were connected to the Croup Monitor by communication method of RS-485 type and could operate under power and communication fault condition within 10 hours. The Croup Monitor was developed to receive and display measurement data received from the House Monitors and to control the greenhouse environmental devices. 3. The images of the plants inside greenhouse were captured by PC camera and sent to the Group Monitor. The greenhouse manager was able to monitor the growth state of plants inside greenhouse without visiting individual greenhouses. 4. Remote monitoring the greenhouse environment and status of control devices was implemented in a client/server environment. The client monitor of the greenhouse manager at a remote site or other greenhouse manager was able to monitor the greenhouse environment and the state of control devices from the Server Monitor using internet.

• Journal of Biosystems Engineering
• /
• v.37 no.2
• /
• pp.122-129
• /
• 2012

Purpose: Recent mechatronics technology is the most appropriate high technology in agricultural applications to save repetitious labor. Method: Cow's body parameters were measured by several traditional measurers. Image processing technology was used to measure automatically their parameters to reduce labor and time. The parameters were measured form a small model cow which is easily measured, instead to a real cow. The image processing system designed and built for this project was composed of a PC, grabber card, and two cameras, which are located on the side and the top of the model cow. Tests of verification had measured 10 dairy cows. Result: Nine parameters of the model cow's body were measured, and the difference between the real data and the data by image processing was less than 16.7%. Based on the results of the research, the parameters of a real cow had measured of chest depth, withers height, Pelvic arch height, body length, slope body length, chest width, hip width, thurl width, and pin bone width were compared with image processing data. Conclusions: In the Demonstration test, Result had obtained similar data of cow model experiments, and the most of errors were shown less than 5% relatively good result.

• Journal of Microbiology and Biotechnology
• /
• v.6 no.6
• /
• pp.445-450
• /
• 1996

Gelatinase A is a member of the matrix metalloproteinases that play an important role in cancer invasion and metastasis. In the course of screening gelatinase A inhibitors from microbial sources, a fungal strain PT-262 showed a strong inhibitory activity. The strain was identified as Chaunopycnis alba on the basis of its morphological characteristics. The inhibitor was isolated from acetone extract of mycelial cake by sequential chromatographies on MCI-gel, Sephadex LH-20, and a reverse-phase HPLC column. The purified inhibitor was identified as pyridoxatin by its physico-chemical properties and spectroscopic analysis. Pyridoxatin is not a peptide analog and has cyclic hydroxamic acid moiety. It inhibited activated gelatinase A with an $IC_{50}$ value of 15.2 ${\mu}M$ using fluorescent synthetic peptide. It also had a strong cytotoxicity against human cancer cell lines in vitro. Furthermore, this compound inhibited DNA synthesis with an $IC_{50}$ value of 2.92 ${\mu}M$ in PC-3 prostate cancer cells by [$^3H$]thymidine incorporation assay.

• Bulletin of the Korean Chemical Society
• /
• v.30 no.7
• /
• pp.1463-1469
• /
• 2009

Eleven novel oxiranyl and thiiranyl phenolic compounds were synthesized as potential antitumor agents using epichlorohydrin and epithiohydrin in the presence of $K_2CO_3$. Cytotoxicities were found in range of I$C_{50}$ values of 2.5-14.8 $\mu$M, which was partially attributed to topoisomerase II inhibition. Bis-thiiranyl anthraquinone analog, 19 showed more cytotoxicity against MDA-MB-231 (breast cancer cell) and PC3 (prostate cancer cell) after 24 and/or 48 h and more potent topoisomerase II inhibitory activity than etoposide.

• Journal of Korean Academy of Nursing Administration
• /
• v.10 no.3
• /
• pp.291-298
• /
• 2004

Purpose: This study is to investigate if the effectiveness training program, suggested by Gordon(1970), could help these nursing students with the problems of interpersonal relationship and self-identification. Method: This study was designed using nonequivalent control group protest-posttest design. The subject for this study were thirty nursing students. Fifteen nursing students of the experimental group experienced the Effectiveness Training for 8 weeks (3 hours/week). The effect of Effectiveness Training was measured using Relationship Change Scale devised by Lee, H.D. & Moon, S.M.(1979) and Ego Identity Scale devised by Dignan(1965) which was translated by Seo, B.Y.(1975) Pretest data were collected by the researcher from both experimental and control group before training. After the end of eight weeks training, posttest data collected from both experimental and control group. Samples were analyzed using SPSS PC+. Result: 1. The first hypothesis, "level of interpersonal relationship of the experiment group after 8 weeks will be significantly higher than that of the control group"(z=-1.965, p=.049)was supported. 2. The second hypothesis, "level of ego-identity of the experimental group after 8 weeks will be significantly higher than that of the control group"(z=-2.191, p=.028)was supported. 3. The third hypothesis, "level of interpersonal relationship of the experiment group will be significantly higher than that of the control group after 4 months of training"(z=-.634, p=.238)was not supported. 4. The fourth hypothesis, "level of ego-identity of the experimental group will be significantly higher than that of the control group after 4 months of training"(z=-.292, p=.642)was not supported. Conclusion: The Effectiveness Training can be considered as an effective method of nursing students's interpersonal relationship & ego-identity, because it was proved to help nursing students increase level of their interpersonal relationship & ego-identity but it was needed to reinforce for continuing of the effect of the interpersonal relationship & ego-identity.

• Animal Bioscience
• /
• v.35 no.11
• /
• pp.1689-1697
• /
• 2022

Objective: The aim of this study was to compare the effects of three kinds of organic acid (OA) products on the growth performance, intestinal characteristics and morphology, and cecal microflora in broilers fed a corn-soybean meal meal diet. Methods: A total of 420 one-day-old male Cobb 500 broilers with an average initial body weight of 49.11±1.02 g were used in this 42-day experiment. Birds were randomly allotted to one of five treatments (7 replicates with 12 birds per replicate). Treatments consisted of negative control (NC), basal diet; positive control (PC), basal diet+100 mg/kg of Aviramycin; OA1, basal diet+500 mg/kg of OA product 1; OA2, basal diet+1,000 mg/kg of OA product 2; and OA3, basal diet+1,200 mg/kg of OA product 3. Results: The results indicated that OA product addition had no effect on growth performance parameters, such as body weight gain, feed intake, and feed conversion ratio, from days 1 to 14, 15 to 28, and 0 to 42, or on the pH values of the intestine, intestinal weight, or intestinal weight to body weight ratio. The intestinal morphology in terms of villus height and crypt depth were affected by dietary supplementation of OA products, respectively. Furthermore, dietary addition of OAs had positive influences on the maintenance of the cecal microflora based on the results of 16S rRNA analysis. Conclusion: Dietary inclusion of three kinds of OA products all benefit broilers, but the mode of action may be different. This study provides a basis for the application of OA products used in the poultry industry.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.44 no.2
• /
• pp.200-207
• /
• 2015

Various natural products or their derivatives, mostly originating from plants, fungi, and bacteria, have been exploited as therapeutic drugs to treat various human diseases. In addition to previously explored organisms, research on natural compounds has now expanded into unexamined living organisms in order to identify novel bioactive substances. Here, we determined whether or not the larval form of the mealworm beetle Tenebrio molitor, a species of darkling beetle, contains cytotoxic substances that exclusively affect cancer cell viability. Ethanol extract and its solvent partitioned fractions, hexane and ethyl acetate fractions, showed anticancer effects against various human cancer cells derived from the prostate (PC3 and 22Rv1), cervix (HeLa), liver (PLC/PRF5, HepG2, Hep3B, and SK-HEP-1), colon (HCT116), lung (NCI-H460), breast (MDA-MB231), and ovary (SKOV3). Cell death induced by the fractions was a mix of apoptosis, necrosis, and autophagy. The hexane fraction was administered intraperitoneally to nude mice bearing a hepatocellular carcinoma SK-HEP-1 and showed inhibition of tumor growth in vivo. Therefore, we concluded that worm extracts contain cytotoxic substances, which can be enriched by proper fractionation protocols, and further separation and purification could lead to the identification of novel molecules to treat human cancers.

• Composites Research
• /
• v.30 no.2
• /
• pp.116-125
• /
• 2017

The purpose of this study is to develop silicon carbide fiber showing an excellent mechanical properties under highly oxidative conditions at high temperature. Polycarbosilane(PCS) as a preceramic precursor was used for making the SiC fiber. PCS fiber was taken by melt spinning method followed by melting the PCS at $300{\sim}350^{\circ}C$ in N2 gas. The Curing of PCS fiber was carried out in air oxygen chamber, prior to high temperature pyrolysis. Degree of cure was calculated by characteristic peak's ratio of Si-H to $Si-CH_3$ in FT-IR spectra before and after curing of PCS fiber. The properties of SiC fiber was affected greatly by the degree of cure. The SiC fiber produced by controlling fiber tension during heat treatment showed good properties. The SiC fiber exposed to $1000^{\circ}C$ at air from 1 min. up to maximum 50 hrs showed around 60% reduction in tensile strength. We found that large amount of carbon content on the fiber surface after long-term exposure has resulted in lower tensile strength.

• Journal of rehabilitation welfare engineering & assistive technology
• /
• v.2 no.1
• /
• pp.35-43
• /
• 2009

In this paper, we introduce a reading training program offering visual-auditory cue with noise cancellation function (RT program) developed by us. The RT program provides some training sentences with visual-auditory cues. Motor speech disorder patients can use the visual and/or auditory cues for reading training. To provide convenient estimation of training result, we developed a noise cancellation algorithm. The function of the algorithm is to remove noise and auditory-cues which are recorded with reading speech at the same time while patient read the sentences in PC monitor. In addition, we developed a function for finding out the first starting time of reading sound after a patient sees a sentence and begins to read the sentence. The recorded speeches are acquired from six people(three male, three female) in four noisy environments (interior noise, white noise, car interior noise, babble noise). We evaluated the timing error for starting time between original recorded speech and processed speech in condition of executing noise cancellation function and not executing. The timing error was improved as much as $4.847{\pm}2.4235[ms]$ as the effect of noise cancellation. It is expected that the developed RT program helps motor speech disorder patient in reading training and symptom evaluation.