• Proceedings of the KSEEG Conference
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• 2003.04a
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• pp.76-79
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• 2003

국내의 토양오염 공정시험방법에서는 Zn, Ni 추출시 산분해법에 가까운 방법을 사용하는 반면, Cd, Cu, Pb, $Cr^{6+}$ 추출시 0.1N HCl용액으로 산처리하여 1시간을 진탕한 후 이를 필터로 여과하여 분석용액을 추출하는 용출법을 사용하고 있다(환경부, 2001). 시료내에는 완충 물질이 존재하기 때문에 용출법 사용시 초기 pH 인 1(0.1N HCl)이 유지되지 않아 완충능력이 높은 토양의 경우 현재 국내 공정법상의 용출법이 중금속 오염정도를 추정하는데 적절치 않을 수 있다. (중략)

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.5
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• pp.273-288
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• 1991

To elucidate the physiological and biochemical differences between chondrichthyes and osteichthyes, the properties of the specific digestive enzymes in cat-shark, Cephaloscyllium umbratile, and mackerel, Scomber japonicus, were studied. Homogenous trypsin proved through the disc-electrophoresis, SDS-PAG electrophoresis and gel filtration was obtained from the pancreas of cat-shark by $50-70\%$ saturated ammonium sulphate fractionation, DEAE-Sephadex A-50 column chromatography, benzamidine-Sepharose 6B affinity chromatography and Sephadex G-75-120 gel filtration. Two types of trypsins were also obtained from the pyloric caeca of mackerel by $30-70\%$ saturated ammonium sulphate fractionation and the slightly modified procedure from the method adopted in the purification of cat-shark trypsin. The two trypsins, designated trypsin A and B, were proved their homogeneity by disc- and SDS-PAG electrophoresis and gel filtration. The molecular weights of the trypsins were estimated to be 31,700 for cat-shark trypsin, 30,000 for mackerel trypsin A and 29,000 for mackerel trypsin B by SDS-PAG electrophoresis, but those were estimated to be 21,500 for cat-shark trypsin, 23,700 for mackerel trypsin A and 21,500 for mackerel trypsin B by gel filtration. The trypsins exhibited their optimum conditions at pH 9.0 and on temperature ranged from $45^{\circ}C\;to\;50^{\circ}C$ for cat-shark, and at pH 8.0 and a temperature of $50^{\circ}C$ for mackerel trypsin A and B, respectively. The cat-shark trypsin was stable at pH 10.0 and the temperature below $10^{\circ}C$, whereas the mackerel trypsin A and B, were stable in the range over pH 7.0 to pH 9.0 below $10^{\circ}C$ and at pH 8.0 below $35^{\circ}C$, respectively. The mackerel trypsins were severely inhibited by some heavy metal ions such as $Ag^{2+},\;Cu^{2+}\;and\;Hg^{2+}$ compared to cat-shark trypsin. All of the enzymes were also inhibited by antipain, leupeptin, TLCK(tosyllysine chloromethyl ketone) and SBTI(soybean trypsin inhibitor) remarkably. The inhibitory effects of PMSF(phenylmethane sulphonylfluoride), DFP(diisopropyl fluorophosphate) and benzamidine were indicated that these enzymes belong to serine-proteases.

• Journal of the Korean GEO-environmental Society
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• v.11 no.1
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• pp.19-26
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• 2010

The batch experiments and response surface methodology (RSM) have been applied to the investigation of the cadmium (Cd) adsorption by coal fly ash (CFA). CFA having maximum Cd removal mass of 8.51 mg/g were calculated from Langmuir model. Cd removal reaction with different initial pH ranged from 4 to 9. When the initial pH was higher, Cd was removed more by adsorption and precipitation. These results suggest that the lower pH cause an increase of $H^+$ ion concentration which competed with Cd ions for exchange sites in CFA. Also, The Cd adsorption was mathematically described as a function of parameters initial Cd concentration ($X_1$), initial pH ($X_2$), and initial CFA mass ($X_3$) being modeled by use of the Box-Behnken methods. Empirical models were developed to describe relationship between the experimental variables and response. Statistical analysis indicates that tree factors ($X_1$, $X_2$, and $X_3$) on the linear term (main effects), and tree factors ($X_1X_2$, $X_1X_3$, and $X_2X_3$) on the non-linear term (Interaction effect; cross-product) had significant effects, respectively. In this case, the value of the adjusted determination coefficient (adjusted $R^2=0.9280$) was closed to 1, showing a high significance of the model. Statistical results showed the order of Cd removal at experimental factors to be initial initial pH > initial Cd concentration > initial CFA mass.

• Journal of fish pathology
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• v.11 no.1
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• pp.77-81
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• 1998

By standardized method, Bacillus subtilis BGA, Bacillus cereus var. mycoides ATCC 11778, and Micrococcus luteus ATCC 9341 were seeded on the muller hinton agar (Difco) plate, and pH was adjusted to 6.0, 7.2, and 8.0. Five agar plates, B. subtilis (pH 6.0), B. cereus (pH 6.0), B. subtilis (pH 7.2), B. subtilis (pH 8.0), and M. luteus (pH 8.0), were employed as test plates of modified EEC 4-plate method. Oxytetracycline (OTC) with a diet was orally administered to flounder, Paralichthys olivaceus, at 100 mg/kg once a day. After oral administration, modified EEC 4-plate method by the three screening test using muscle-direct, extraction-disk and direct-disk methods was conducted for 3 fish at 1, 3, 5, 10, 15, 20, 25 and 30 days. Muscle-direct treatment of B. subtilis (pH 6.0) was found to be dubious positive (${\pm}$) at the 1st day after the administration; thereafter, it was found to be negative to the last day of the experiment. Extraction-disk and direct-disk treatment of B. subtilis (pH 6.0) were found to be negative from the 1st day to the last day after the administration. B. subtilis (pH 7.2), B. subtilis (pH 8.0), and M. luteus (pH 8.0) by the three screening tests, were found to be negative all the way after the administration. On the other hand, B. cereus (pH 6.0) by the three screening tests was clearly found to be positive for the first 15 days after the administration, and then muscle-direct and direct-disk treatment of B. cereus (pH 6.0) were found to be dubious positive at 20th days after the administration. However extraction-disk treatment of B. cereus (pH 6.0) was clearly found to be negative at the same stage; thereafter, the three screening tests of B. cereus (pH 6.0) were found negative to the last of the experiment. These findings showed that to have equal sensitivity to those determination for the residual detection of OTC, and also confirmed that B. cereus was effective test organism for the monitoring of OTC.

• Journal of the Korean Chemical Society
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• v.41 no.8
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• pp.399-405
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• 1997

We examined the determination of trace amount of germanium in rocks and sediments by hydride vapor generation-ICP-AES. Germanium is formed volatile compounds with various types of acid reagents, but volatilizing of germanium was decreased in the presence of $H_3PO_4$. Sediments and rocks can be dissolved by mixed acids of $HF-HNO_3-H_3PO_4$ without volatilizing loss of germanium in open digestion system and it was possible to determine germanium by hydride generation-ICP-AES without further sample treatment. Detection limit of Ge is reached to 0.08 ppb under the condition of 5M $H_3PO_4$ and 1% $NaBH_4$ as a supporting acid and a reducing reagent, respectively. The measured values by hydride generation-ICP-AES agreed well with the reference values of SRMs as well as the values determined by solution nebulization-ICP-MS.

• Korean Journal of Soil Science and Fertilizer
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• v.26 no.3
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• pp.160-171
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• 1993

Surface charge characteristics of Ultisol(Luisiana soil from Philippines), Alfisol(Maahas soil from Philippines), and Inceptisol(Yongii soil from Korea) were studied by way of potentiometric titration, backtitration technique, and ion adsorption method(or CEC - AEC method). The PZNC(point of zero net charge) values determined by ion adsorption method were much lower than the natural pHs in all soils, indicating that all soil samples bore net negative surface charge. The PZSE (point of zero salt effect) values determined by potentiometric titration and backtitration technique were identical in Luisiana and Yongii soils but not in Maaghas soil. All soils showed higher PZSE values than PZNC values probably due to the influence of permanent negative charge. The permanent charge calculated by the theory of Uehara and Gillman (1980) occupied quite low portion of the CEC measured at pH 7 in all soils. Backtitration technique corrected errors of potentiometric titration at extreme pH. However, it still overestimate the surface charge compared with ion adsorption method. Therefore, the ion adsorption method was recommanded for the surface charge measurement of the usual soils which have high negative charge components.

• Journal of Advanced Navigation Technology
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• v.9 no.1
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• pp.41-47
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• 2005

In this paper, the performances of various channel estimation schemes for DVB-H system are investigated. The linear interpolation, the second order interpolation, the cubic spline interpolation, and the time-domain interpolation, which are used in frequency domain, are chosen as the channel estimation schemes. We derived the performances of Mean Square Error (MSE), Bit Error Rate (BER), and the complexity of calculation in Rayleigh and Rician fading channel through computer simulations. From the simulation results, the cubic spline interpolation shows the best performance under high $E_b/N_0$ environments.

• 한국신재생에너지학회:학술대회논문집
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• 2007.11a
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• pp.577-580
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• 2007

분자 크기가 너무 커 가스 하이드레이트를 형성하지 않는다고 알려진 n-펜탄과 n-헥산이 다른 구조-H 형성 화합물과 혼합되어 사용될 경우 구조-H 동공 내에 함께 포접되는 것으로 확인되었다. 구조-H 하이드레이트의 형성 및 미세구조 분석은 고체 NMR 및 X-선 회절 분광법을 이용하여 확인하였다. 이러한 혼합 화합물에서 보이는 구조-H 하이드레이트 형성은 전체적인 구조-H 형성 화합물에서 나타나는 일반적 특징인 것으로 여겨진다.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.417.2-417.2
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• 2014

금 나노입자의 특성은 그 크기와 모양 그리고 균일한 정도에 의해 결정되므로, 균일한 크기의 금 나노입자를 사용하는 것이 매우 중요하다. Citrate 환원법으로 금 나노입자 합성 시 입자의 크기 분포에 가장 큰 영향을 주는 요인은 pH 이고, 반응용액의 pH를 높이면 크기가 균일한 금 나노입자의 합성이 가능함을 선행연구를 통해 확인한 바 있다[1]. 본 연구는 금 나노입자 형성 반응이 진행됨에 따라 나타나는 pH 변화를 실시간으로 추적하여 pH 변화가 금 나노입자의 균일도에 미치는 영향을 관찰한 것이다. 반응용액의 pH는 반응이 진행됨에 따라 지속적으로 변하는데, 반응초기에 pH가 감소하다가 (Stage I) 전환점 이후 pH가 증가하는 (Stage I) 양상을 보인다. 이러한 현상은 Au 이온의 리간드가 Cl-에서 OH-로 변화하기 때문으로 생각되고, 이로 인해 Stage I의 핵 형성반응과 Stage II의 성장반응에 영향을 주게 되어 결과적으로 입자의 크기 균일성에 영향을 주는 것으로 판단된다.

• Analytical Science and Technology
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• v.12 no.4
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• pp.299-305
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• 1999

The electrolyte cathode atomic glow discharge (ELCAD) is a new plasma source for direct determination of trace heavy metals in drinking and waste water. ELCAD has been successfully developed for on-line monitoring of heavy metals, however, shows difficulty to measure mercury. In this study, ELCAD has been modified to apply the atomic absorption spectrometry (AAS) for the direct determination of trace elements of mercury in flowing water.The fundamental characteristics of this new types of plasma source have been investigated and found that the pH of the solution, discharge voltage, and current are most important factors.The absorbance of 1.0 ppm Hg standard solution increases as pH of the solution increases from pH 1.0 to 3.0.However, % RSD of the absorbance also increases as the pH of solution increasesdue to plasma unstability.The detection limits of the standard solution of pH 1.5 and pH 3.0 are about 40 ppb and 10 ppb level, respectively.