Journal of fish pathology (한국어병학회지)
- Volume 11 Issue 1
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- Pages.77-81
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- 1998
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- 1226-0819(pISSN)
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- 2233-5412(eISSN)
A Comparative Study of the Detectable Methods of Residual Oxytetracyeline in Muscle of Flounder (Paralichthys olivaceus) with Simplified Screening Test
넙치 근육중 잔류 옥시테트라싸이클린의 간이스크리닝 검출방법 비교연구
- Jung, Sung-Hee (Pathology Division, National Fisheries Research and Development Institute) ;
- Kim, Jin-Woo (Pathology Division, National Fisheries Research and Development Institute)
- Published : 1998.05.30
Abstract
By standardized method, Bacillus subtilis BGA, Bacillus cereus var. mycoides ATCC 11778, and Micrococcus luteus ATCC 9341 were seeded on the muller hinton agar (Difco) plate, and pH was adjusted to 6.0, 7.2, and 8.0. Five agar plates, B. subtilis (pH 6.0), B. cereus (pH 6.0), B. subtilis (pH 7.2), B. subtilis (pH 8.0), and M. luteus (pH 8.0), were employed as test plates of modified EEC 4-plate method. Oxytetracycline (OTC) with a diet was orally administered to flounder, Paralichthys olivaceus, at 100 mg/kg once a day. After oral administration, modified EEC 4-plate method by the three screening test using muscle-direct, extraction-disk and direct-disk methods was conducted for 3 fish at 1, 3, 5, 10, 15, 20, 25 and 30 days. Muscle-direct treatment of B. subtilis (pH 6.0) was found to be dubious positive (
실험에는 Bacillus subtilis BGA, Bacillus cereus var. mycoides ATCC 11778, Micrococcus luteus ATCC 9341을 상법에 따라 제작하여 muller hinton agar(Difco)에 각각 접종한 뒤, 3가지 조건의 pH가 조정된 검사용 평판배지(plate)인 B. subtilis(pH 6.0), B. cereus(pH 6.0), B. subtilis(pH7.2), B. subtilis(pH 8.0), M. luteus(pH 8.0)의 EEC 4-plate변법을 이용하였다. 넙치에 옥시테트라싸이클린(OTC)을 농도 100 mg/kg/day으로 단 1회 강제 경구 투여한 후 1일, 3일, 5일, 10일, 15일, 20일, 25일 30일마다 3마리씩 채취하여 시료의 처리를 각각 달리한 근육직접법, 추출디스크법, 직접디스크법의 3종류 간이스크리닝법으로써 OTC의 잔존유무를 분석하였다 B. subtilis(pH 6.0)배지는 투여 후 1일째 근육직접법에서만 양성반응으로 의심되는 의양성 반응(
Keywords
- Flounder;
- Paralichthys olivaceus;
- Oral administration;
- Oxytetracycline;
- Screening method;
- EEC 4-plate method