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A Comparative Study of Endotracheal Aspirates and Protected Specimen Brush in the Quantitative Cultures of the Ventilator-Associated Pneumonia (기계호흡기 관련 폐렴환자의 정량적 배양에 있어서 Endotracheal Aspirates과 Protected Specimen Brush의 비교 관찰)

  • Roo, Kyoung-Ryeol;Kim, Min-Gu;Kim, Gi-Yang;Jung, Ho-Gyeong;Park, Young-Ho;Kang, Byeng-Sun;Kim, Ho-Chul;Hwang, Young-Sil
    • Tuberculosis and Respiratory Diseases
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    • v.42 no.5
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    • pp.737-743
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    • 1995
  • Background: Pneumonia is a frequent complication in patients undergoing mechanical ventilation, Quantitative culture of protected specimen brush(PSB) have shown satisfactory diagnostic accuracy for the diagnosis of ventilator-associated pneumonia. However PSB method is invasive, expensive, and require a bronchoscopic procedure. But endotracheal aspiration(EA) is simple and less expensive. The purpose of our study was to investigate the diagnosic value of EA quantitative cultures. Method: We studied 15 cases of ventilator-associated pneumonia(for >72h of mechanical ventilation) patients. Patients were divided into two diagnostic categories. Group I was the patients who were suspicious of clinical pneumonia, Group II was the patients for control. The obtained samples by EA and PSB were homogenized for quantitative culture with a calibrated loop method in all patients. Result: Using $10^3cfu/ml$, $10^5cfu/ml$ as threshold in quantitative culture of PSB, EA respectively, we found that EA quantitative cultures represented a relatively sentive(70%) and relatively specific (60%) method to diagnose the ventilator-associated pneumonia. Conclusion: Although EA quantitative cultures are less specific than PSB for diagnosing ventilator-associated pneumonia. EA quantitative cultures correlated with PSB quantitative culture in patients with clinical pneumonia and may be used to treat these patients when bronchoscopic procedures are not available.

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PREVENTIVE EFFECT OF ADHESIVE TAPE SUPPLEMENTED WITH NaF ON ENAMEL EROSION IN VITRO (불소함유 접착테잎의 법랑질 침식 예방효과)

  • Lee, Sang-Ho;Lee, Nan-Young;Lee, In-Hwa
    • Journal of the korean academy of Pediatric Dentistry
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    • v.37 no.1
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    • pp.82-90
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    • 2010
  • This study examined the effect of adhesive tape supplemented with sodium fluoride on the prevention of dental erosion in vitro. Sound bovine tooth samples were selected and divided randomly into the following 4 groups according to the material treatments: group 1, APF gel; group 2, fluoride varnish; and groups 3 and 4, fluoride tape supplemented with 5% NaF in either a methyl cellulose or poly vinyl acetate carrier, respectively. All specimens were submitted to alternate cycles of acid exposure in a cola beverage (pH 4.3) and artificial saliva for $6\;{\times}\;5\;min/day$ over a 5 day period. The micro-hardness was recorded each day and the lesion depth was measured after 5 days. The micro-hardness of the experimental sides of groups 2, 3 and 4 were significantly higher than that of their control sides and the experimental side of group 1 during the experimental period (p<0.05) except on the 5th day. The enamel surfaces of treatment groups 2, 3 and 4 showed significantly higher resistance to mineral loss in terms of the erosion depth (p<0.05) than group 1 and their control sides. There was no statistically significant difference among group 2, 3 and 4, indicating that the fluoride varnish and tapes produce similar results. Fluoride adhesive tapes are effective in reducing the progression of erosion and can be recommended for young patients who are more susceptible to dental erosion.

Structural and Piezoelectric Properties of MnO2-Doped PZT-PSN Ceramics for Ultrasonic Vibrator (초음파 진동자용 MnO2가 Doping된 PZT-PSN 세라믹스의 구조 및 압전 특성)

  • Cha, Yoo-Jeong;Kim, Chang-Il;Kim, Kyoung-Jun;Jeong, Young-Hun;Lee, Young-Jin;Lee, Hai-Gun;Paik, Jong-Hoo
    • Korean Journal of Materials Research
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    • v.19 no.4
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    • pp.198-202
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    • 2009
  • For use in ultrasonic actuators, we investigated the structural and piezoelectric properties of $(1\;-\;x)Pb(Zr_{0.515}Ti_{0.485})O_3$ - $xPb(Sb_{1/2}Nb_{1/2})O_3$ + 0.5 wt% $MnO_2$ [(1 - x)PZT - xPSN + $MnO_2$] ceramics with a variation of x (x = 0.02, 0.04, 0.06, 0.08). All the ceramics, which were sintered at $1250^{\circ}C$ for 2 h, showed a typical perovskite structure, implying that they were well synthesized. A homogeneous micro structure was also developed for the specimens, and their average grain size was slightly decreased to $1.3{\mu}m$ by increasing x to 0.8. Moreover, a second phase with a pyrochlore structure appeared when x was above 0.06, which resulted in the deterioration of their piezoelectric properties. However, the 0.96PZT-0.04PSN+$MnO_2$ ceramics, which corresponds with a morphotropic phase boundary (MPB) composition in the (1 - x)PZT - xPSN + $MnO_2$ system, exhibited good piezoelectric properties: a piezoelectric constant ($d_{33}$) of 325 pC/N, an electromechanical coupling factor ($k_p$) of 70.8%, and a mechanical quality factor ($Q_m$) of 1779. The specimens with a relatively high curie temperature ($T_c$) of $305^{\circ}C$ also showed a significantly high dielectric constant (${\varepsilon}_r$) value of 1109. Therefore, the 0.96PZT - 0.04PSN + $MnO_2$ ceramics are suitable for use in ultrasonic vibrators.

Physicochemical and Sensory Characteristics of Turnip Pickle Prepared with Different Pickling Spices During Storage (Pickling Spice를 달리하여 제조한 순무 피클 저장 중 이화학적ㆍ관능적 특성)

  • 오상희;오윤경;박현희;김미리
    • Food Science and Preservation
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    • v.10 no.3
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    • pp.347-353
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    • 2003
  • Physicochemical and sensory characteristics of turnip pickle prepared with different pickling spices were investigated. Turnip root slices(4${\times}$1${\times}$0.5 cm) were salted with NaCl and CaCl$_2$, and then soaked into pickling solution added to commercial (pickle P) or manufactured (pickle M) pickling spice, and then stored at 2$0^{\circ}C$. Throughout the whole storage periods, the acidity, pH, saltiness and reducing sugar content of two pickles were 3.1∼3.5%, 1.4∼1.7, 0.2∼0.6% and 25.2∼30.4 mg/mL, respectively. There were no significant differences between two pickles in saltiness, anthocyanin, reducing sugar content, color(Hunter L, a and b value) and hardness. However, acidity of pickle M was higher than that of pickle P throughout the storage time. Sensory results showed that the best edible time was the 14th day of storage, and at that time, scores of pickle M was higher in 리avor(7.3 and 6.9), taste(7.8 and 5.4) and over-all preference(8.0 and 6.1) than those of pickle P, and pickle M maintained good sensory qualities until 28th day of storage, compared to pickle P(p<0.05).

Antioxidative and Cytotoxicity Activities against Human Colon Cancer Cells Exhibited by Edible Crude Saponins from Soybean Cake (대두박 식용사포닌의 항산화 및 대장암세포 성장 억제효과)

  • Park, Kyung-Uk;Kim, Jae-Yong;Seo, Kwon-Il
    • Food Science and Preservation
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    • v.16 no.5
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    • pp.754-758
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    • 2009
  • To develop soybean cake as a functional food material, the anti-oxidative and cytotoxic activities against human colon cancer cells of crude saponins isolated from 70% (v/v) ethanol extracts of cake were investigated. The Diaion HP-20 adsorption method was used for isolation of crude saponins, which were then eluted with 100% ethanol. The non-saponin fraction was removed by elution with $H_2O$ and 20% (v/v) ethanol. The results of thin layer chromatography (TLC) analysis confirmed that crude saponins were present in the 100% ethanol extract of soybean cake. The hydrogen-donating properties of saponins were more than 60% at a concentration of $1,000\;{\mu}g/mL$. malondialdehyde(MDA) production was $1,200\;{\mu}mol\;MDA/g$ in mouse liver homogenate treated with crude saponins at the concentration of $1,000\;{\mu}g/mL$. This value was lower than that of the control, which was $3,700\;{\mu}mol\;MDA/g$. Saponins inhibited the growth of colon cancer cells in a dose- and time-dependent manner. Saponins also resulted in a decrease in the proportion of cells in the G1 phase of the cell cycle, whereas the cell proportion in G2/M phase was increased with $1,000\;{\mu}g/mL$ saponins. Thus, we conclude that saponins may induce G2/M cell cycle arrest.

Effect of Meju Shapes and Strains on the Quality of Soy Sauce (Aspergillus oryzae 및 Aspergillus sojae를 이용한 개량(改良)메주의 형상에 의한 장류(醬類)의 품질비교)

  • Kim, Sang-Soon
    • Korean Journal of Food Science and Technology
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    • v.10 no.1
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    • pp.63-72
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    • 1978
  • Effect of shapes (noodle, grain and brick types) and strains (Aspergillus oryzae and Aspergillus sojae) of Meju (microorganism inoculated soybean substrate for fermentation) on the quality of soy sauce was investigated. Generally, the highest protease activity was found in the noodle type-Meju inoculated Asp. sojae during Meju preparation and soy sauce brewing, however, the lowest value was noted in brick type. Meju inoculated Asp. oryzae. Similar tendency was found on the contents of total solid, total nitrogen, amino-nitrogen, nitrogen digestion yield and amino-nitrogen ratio during soy sauce brewing. No effect was shown on the reducing sugar content, alcohol formation, pH, buffer action and salt content according to different Meju types during soy sauce brewing. Organoleptic tests on the color, taste and flavor of soy sauce after 3 months brewing with various Meju scored in the order of noodle type-Asp. sojae soy sauce (best quality), noodle type-Asp, orzae soy sauce, brick type-Asp. sojae soy sauce and brick type-Asp. oryzae-soy sauce (worst quality).

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Cooking Quality and Texture of Japonica-Indica Breeding Type and Japonica Type, Korean Rice (한국산(韓國産) 미곡(米穀), 통일계(統一系)와 일반계(一般系)의 취반특성(炊飯特性) 및 Texture 에 관(關)한 연구(硏究))

  • Lee, B.Y.;Yoon, I.H.;Tetsuya, Iwasaki;Ikuji, Kamoi;Tetsujiro, Obara
    • Korean Journal of Food Science and Technology
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    • v.21 no.5
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    • pp.613-618
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    • 1989
  • Tirteen rice varieties of Japonica-Indica briefing type (J-I breeding type) and ten of Japonica type produced in Korea in 1985 were studied on cooking quality of milled rice and texture of cooked rice. The results are as follows: in the Looking quality, water uptake ratio of cooked rice and total solid in residual liquid showed significant $(p<2.172^*\;and\;2.419^*,\;respectively)$ between J-I breeding type and Japonica type The water uptake ratio of cooked rice was higher J-I breeding type than Japonica type but the total solid and intensity of iodine blue value in residual liquid were lower J-I breeding type than Japonica type. Minimum cooking time of milled rice showed significant $(p<2.375^*)$ between J-I breeding type and Japonica type for texture of cooked rice. The cooking time of J-I breeding type shorter than Japonica type. Hardness and cohesiveness in texture of cooked rice appeared to be significant cant $(p<4.370^{***}\;and\;2.371^*,\;respectively)$ between J-I breeding type and Japonica type, and then hardness was higher Japonica type than J-I breeding type, but cohesiveness was lower Japonica type than J-I breeding type. The correlation coefficients between amylose content and adhesiveness of varieties in the same type was $-0.922^{**}$ in J-I breeding type and $-0.915^**}$ in Japonica type. The correlation coefficients between protein content and hardness of the varieties in the same type was $0.954^{**}$ in Japonica type and so denoted as positive correlation. And the scatter diagram between protein and hardness could be divided into J-I breeding type and Japonica type.

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Effects of High Hydrostatic Pressure Treatment on the Chemical Composition of Germinated Rough Rice (Oryza sativar L.) (고압처리가 발아벼의 화학성분 변화에 미치는 영향)

  • Kim, Min Young;Lee, Sang Hoon;Jang, Gwi Yeong;Park, Hye Jin;Yoon, Nara;Lee, Youn Ri;Lee, Junsoo;Jeong, Heon Sang
    • Korean Journal of Food Science and Technology
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    • v.47 no.2
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    • pp.198-203
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    • 2015
  • This study was performed to evaluate changes in the chemical composition of germinated rough rice with high hydrostatic pressure treatment (HPT). Rough rice was germinated at $37^{\circ}C$ over 6 days (control), and then subjected to HPT at 30 MPa for 24 h. The highest crude protein content was 9.54% in the control sample after 6 days of germination. Crude lipid content increased from 2.04-2.74% (control) to 2.27-3.10% (HPT). HPT samples showed higher values of total free sugar and glucose content than those of the control. The total amino acid value was not significant, but the essential amino acid content increased from 0.45-5.09 mg/g in the control to 1.57-5.30 mg/g in the HPT sample. The major fatty acids were found to be palmitic, oleic, and linoleic acid. The content of oleic acid decreases with HPT, whereas that of linoleic and linolenic acid increased slightly during the initial stages of germination. These results suggest that HPT after germination efficiently depolymerizes chemical components and enhances the content of essential nutrients.

Physico-Chemical Characteristics of Aquacultural Discharging Water in Jeju Island (제주도내 양식장 배출수의 이화학적 특성 분석)

  • Kim, Man-Chul;Jang, Tae-Won;Han, Yong-Jae;Kim, Ju-Sang;Harikrishnan, Remasamy;Oh, Duck-Chul;Kim, Ki-Young;Heo, Moon-Soo
    • Journal of Life Science
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    • v.19 no.7
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    • pp.943-948
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    • 2009
  • Physical and chemical analyses of water discharged from 4 crowded farms (Sungsan, Pyosun, Wimi and Daejung) in Jeju island were performed from July, 2006 to Dec, 2006, and the result of the analyses showed that hydrogen ion concentrations (pH) for water discharged from Sungsan farm was 7.74, Pyosun was 7.68, Wimi was 7.68 and Daejung was 7.7. Salinity levels for Sungsan, Pyosun and Wimi had an average of 31$\sim$33 $^\circ$/$_\circ$$_\circ$ indicating characteristics of far distance areas, whereas that of Daejung was 28.81 %, which was far lower compared to regular sea water salinity. As the result of measuring dissolved oxygen (DO) for each area, each area showed first graded DO for each discharged water based on water quality level for each sea district. The result of measuring the temperature for discharged water showed that water temperatures for summer were 23$\sim$25$^\circ$C, and those for winter were 16$\sim$ 18$^\circ$C. Nitrogen concentrations for discharged water exceeded each sea area's water quality level in all farms. In the case of phosphate, its average value was 0.48 mg/l for Sungsan, 0.55 mg/I for Pyosun, 0.66 mg/I for Wimi, and 0.44 mg/l for Daejung, and chemical oxygen demand (COD) was shown to be 1.5 mg/l 1.8 mg/I, 1.6 mg/I and 2.3 mg/I for Sungsan, Pyosun, Wimi and Daejung respectively. For suspended solids (SS), the average concentration was 19.3 mg/I, 21.2 mg/I, 21.3 mg/I and 18.5 mg/I for Sungsan, Pyosun, Wimi and Daejung respectively. The results of physical and chemical analyses for discharged water in farms based on time showed that almost all items were shown to increase in the forenoon and decrease, overall, in the afternoon.

Impulse Response Filtration Technique for the Determination of Phase Velocities from SASW Measurements (SASW시험에 의한 위상속도 결정을 위한 임펄스 응답필터 기법)

  • ;Stokoe, K.H., Il
    • Geotechnical Engineering
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    • v.13 no.1
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    • pp.111-122
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    • 1997
  • The calculation of phase velocities in Spectral-Analysis -of-Surface -Waves (SASW) meas urements requires unwrapping phase angles. In case of layered systems with strong stiffness contrast like a pavement system, conventional phase unwrapping algorithm to add in teger multiples of 2n to the principal value of a phase angle may lead to wrong phase volocities. This is because there is difficulty in counting the number of jumps in the phase spectrum especially at the receiver spacing where the measurements are in the transition Bone of defferent modes. A new phase interpretation scheme, called "Impulse Response Fil traction ( IRF) Technique," is proposed, which is based on the separation of wave groups by the filtration of the impulse response determinded between two receivers. The separation of a wave group is based on the impulse response filtered by using information from Gabor spectrogram, which visualizes the propagation of wave groups at the frequency -time space. The filtered impulse response leads to clear interpretation of phase spectrum, which eliminates difficulty in counting number of jumps in the phase spectrum. Verification of the IRF technique was performed by theoretical simulation of the SASW measurement on a pavement system which complicates wave propagation.opagation.

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