• Development and Reproduction
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• v.16 no.4
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• pp.279-287
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• 2012

We investigated the changes in plasma sex steroid hormones, testosterone (T), estradiol-$17{\beta}$ ($E_2$), 17,$20{\beta}$-dihydroxy-4-pregnen-3-one ($17{\alpha}20{\beta}P$), 11-ketotestosterone (11KT) and cortisol levels from ribbed gunnel, Dictyosoma burgeri in associated with annual reproductive cycle. The gonadosomatic index (GSI) of females increased from November, peaked in February and decreased rapidly from March. The GSI of males also increased from November, peaked in January and then decreased gradually. In females, $E_2$ levels increased and remained high from December to February. The levels of T showed a similar tendency and correlated ($r_s$=0.898, p<0.01) with $E_2$ levels. The levels of $17{\alpha}20{\beta}P$ increased rapidly in February ($4.78{\pm}1.01ng/ml$) and peaked in July ($5.08{\pm}0.65ng/ml$). Cortisol level was peaked in March and correlated with $17{\alpha}20{\beta}P$ levels ($r_s$=0.696, p<0.01). In males, the levels of T was peaked in January and then decreased rapidly. The levels of 11KT were remained high from October to January. On the other hand, the levels of $17{\alpha}20{\beta}P$ fluctuated during reproductive cycle. These results suggest that plasma sex steroids in ribbed gunnels have annual periodicity, and that cortisol may involve in maturation of females.

• Development and Reproduction
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• v.14 no.2
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• pp.115-121
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• 2010

The in vitro effects of nonylphenol (NP) and 2,2',4,6,6'-pentachlorobiphenyl (PCB104) on ovarian steroidogenesis of the ribbed gunnel, Dictyosoma burgeri were investigated. Oocytes taken during maturation stage were incubated with 100 ng/$m{\ell}$ of NP and PCB104 in the presence of exogenous precursor, $[^3H]-17{\alpha}$-hydroxyprogesterone ($[^3H]-17{\alpha}OHP$). Steroids were extracted from the media and the isolated oocytes, and the extracts were separated and identified by thin layer chromatography. The identities of the major metabolites were testosterone (T) and estradiol-$17{\beta}$ (E2). NP treatment inhibited production of E2 metabolite in the oocytes of 1.2, 1.3 and 1.4 mm although NP inhibited production of T metabolite at the oocytes of 1.1, 1.3 and 1.4 mm. PCB104 treatment inhibited production of T metabolite in the oocytes of all groups and E2 metabolite in the oocytes of 1.2, 1.3 and 1.4 mm. In conclusion, these results suggested that NP and PCB104 had an inhibitory effects on conversion of $[^3H]-17{\alpha}OHP$ to T and E2 during the oocyte maturation process of ribbed gunnel.

• Korean Journal of Ichthyology
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• v.13 no.1
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• pp.6-18
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• 2001

Larvae of the gunnel Pholis fangi were collected in coastal waters off Daecheon with a bag net from March to June, 1988, and with a ring larva net in February 1989. Maturity and spawning period were analyzed by examination of the gonads of adult fish collected with a bag net from May 1998 through November 1999. In February, the larvae were widely distributed in the outer and inner Cheonsu Bay. From March to April the larvae were present mainly the inner bay; they were absent there in May and found mainly in the outer bay. After June, few gunnel larvae were collected in the study area. This suggests a seaward movement of gunnel from the nursery grounds of the bay to offshore feeding grounds. The otolith of larvae smaller than 10 mm in total length did not show a distinct growth stop. The growth stop is believed to be formed in the early larval stage when the total length is about 10 mm. This period coincides with the time of shoreward migration, suggesting a metabolic change during this period. At a total length of 30 to 40 mm, the shape of the otolith changes from spherical to elongate. Daily growth rate in length was estimated by the Gompertz equation, which is represented as follows: TL = 6.702exp{2.925"1-exp (-0.008 t)"} ($r^2=0.94$, N = 92) Assuming daily deposition of growth increments in the otolith, the time of first growth increment formation was shown to be from December to January. Gonad observations show that Pholis fangi spawns from November to December. So, the hatching time is thought to be about one month.

• 대한예방의학회:학술대회논문집
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• 2002.10a
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• pp.258-258
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• 2002

• Korean Journal of Ichthyology
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• v.7 no.1
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• pp.25-32
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• 1995

The larval fish, which had been previously identified or classified as Enedrias (Pholis) nebulosus, Enedrias spp., Pholis taczanowskii, and unidentified species belonging to Protosalanginae, were revealed as that of a gunnel, Enedrias fangi. This species has been known as a cold water species mainly caught in the Yellow Sea. Larval E. fangi showed peak abundances in the coastal waters off Chonlla Province in March, Chungnam Province in April, and Kyunggi Province in May and June. The primary spawning season of E. fangi seemed to be winter (November to January), and the eggs hatched after 60 days from spawning. The average growth rate of larval fish was about 0.33mm/day. The larval fish of 40mm in body length began to move to the bottom, and stayed in the bottom cold water mass when their body length was greater than 60-70mm. The average annual catches of larval E. fangi from 1985 to 1991 were 5,000M/T in the Yellow Sea, which were more than 99 % of the total gunnel catches around Korean waters. The peak season of catches was from March or April to July in Chungnam Province, and from May to July in Kyunggi Province.

• Journal of the Korean Geotechnical Society
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• v.16 no.2
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• pp.51-59
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• 2000

네일(또는 락볼트)은 토사터널의 NATM 시공 시 보강재로 사용되어진다. 그러나, 네일의 적절한 설치방법이 아직까지 정립되지 않았다. 본 연구에서는 네일의 길이와 위치를 변화하여, 그 효용성을 연구하였다. 그 결과, 네일이 지반보강을 위하여 토사지반에 사용될 시 경제적인 길이는 터널직경의 0.5배이다. 보강의 효용성은 네일의 위치에 따라 터널라이닝 측벽의 하부, 중부, 상부의 순서이다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.5
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• pp.484-490
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• 1985

Gut contents of larval gunnels collected in Kyonggi Bay, Yellow Sea were examined in order to understand the feeding habit of the fish. There were some differences in the gut contents depending upon the body length of the fish. Most important food organisms were Copepoda followed by Appendicularia, fish eggs and Decapoda larvae. Although major food organisms were closely related to the size of zooplankton population, the fish showed a positive food selectivity for Copepoda with increasing body lengh, while there was a negative selectivity for Chaetognatha regardless of body length. However, there appeared to be no size preference on the food organisms by the larval gunnel.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.16 no.2
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• pp.97-102
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• 1983

Enedrias fangi, which was not yet recorded in Korean fishes fauna, is revealed in Korean coastal waters during the present research period. The external morphology of this species is compared with that of E. nebulosus ('bedorachi' in Korean name). Taking into consideration the morphological differences between two species and the Korean name of S. nebulosus, 'bedorachi', the authors propose 'heenbedorachi' meaning white gunnel as Korean name of this unrecorded species.

• Animal cells and systems
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• v.13 no.4
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• pp.453-460
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• 2009

Morphological and molecular classifications were attempted in an effort to establish species-specific classifications of three species of the genus Pholis in Korea; these species were subjected to morphological and molecular methodologies using body measurements, RFLP, RAPD, and phylogenetic trees using the nucleotide sequences of mitochondrial 16S and 12S ribosomal DNAs, cytochrome c oxidase I, and cytochrome b. The data demonstrated that the three species of genus Pholis are distinct from each other, both morphologically and genetically.

• Journal of Marine Life Science
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• v.9 no.1
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• pp.47-52
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• 2024

Light is a major external environmental factor that influences the circadian rhythm of photosynthetic organisms and various physiological phenomena, such as growth, maturation, and behavior. The number of light-reaching organisms changes depending on the season and atmospheric conditions, and the intensity and wavelength of light differ depending on the organisms inhabiting the environment. Altered light changes the circadian rhythm of fish, which is controlled by clock genes, such as period 2 (Per2), cryptochrome 1 (Cry1), and melatonin. In this study, we set the zeitgeber time (ZT; 14 light-10 dark, LD) based on the actual sunrise and sunset times and examined Per2 and Cry1 activities, levels of aralkylamine N-acetyltransferase (AANAT), and melatonin in Pholis nebulosa, a drifting seaweed species exposed to irregular light. Per2 and Cry1 levels increased during the daytime and decreased after sunset. The AANAT levels decreased during the daytime and increased during the night. Melatonin concentration was highest around midnight (ZT21, 23:30), but exhibited similar concentrations during the daytime. While the activity of Per2, Cry1, and AANAT levels exhibited a typical circadian rhythm observed in most vertebrates, melatonin concentrations did not show a significant difference between the daytime and nighttime. These findings provide insights into the circadian rhythm patterns of organisms exposed to irregular light environments, such as P. nebulosa, which differ from those of typical fish species.