• Molecules and Cells
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• 제21권1호
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• pp.29-33
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• 2006

We have established three immortal bovine muscular epithelial (BME) cell lines, one spontaneously immortalized (BMES), the second SV40LT-mediated (BMEV) and the third hTERT-mediated (BMET). The morphology of the three immortal cell lines was similar to that of early passage primary BME cells. Each of the immortal cell lines made cytokeratin, a typical epithelial marker. BMET grew faster than the other immortal lines and the BME cells, in 10% FBS-DMEM medium, whereas neither the primary cells nor the three immortal cell lines grew in 0.5% FBS-DMEM. The primary BME cells and the immortal cell lines, with the exception of BMES, made increasing amounts of p53 protein when treated with doxorubicin, a DNA damaging agent. On the other hand, almost half of the cells in populations of the three immortal cell lines may lack $p16^{INK4a}$ regulatory function, compared to primary BME cells that were growth arrested by enforced expression of $p16^{INK4a}$. In soft-agar assays, the primary cells and immortal cell lines proved to be less transformed in phenotype than HeLa cells. The three immortal epithelial-type cell lines reported here are the first cell lines established from muscle tissue of bovine or other species.

• 융합정보논문지
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• 제8권6호
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• pp.15-20
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• 2018

본 연구는 뉴로피드백 훈련을 적용하여 생활습관을 조절하는 자기조절력과 키 성장간의 상관성을 규명함으로 써 키 성장의 효과적인 중재 방안에 대한 해법을 제시하고자 실시하였다. 이를 위해 키 성장 프로그램을 실시하고 있는 초등학교 2학년~4학년 학생 40명(실험군 20명, 대조군 20명) 대상으로 뉴로피드백 훈련 전 후의 변화를 확인하였다. 실험기간은 3개월간(주 2회), 훈련시간은 1회 30분이였다. 뉴로피드백 훈련을 적용한 실험군과 대조군의 자기조절력 차이를 분석한 후, 키 성장 차이를 비교 분석하였다. 첫째, 뉴로피드백 훈련을 적용한 실험군이 대조군에 비해 자기조절력에 유의미한 변화가 있었다. 둘째, 뉴로피드백 훈련을 적용한 실험군이 대조군에 비해 더 크게 키 성장의 변화가 있었다. 이상의 결과를 종합하면 뉴로피드백 훈련이 성장기 학생들에게 있어 키 성장의 후천적 요인들 중 생활습관을 조절하는 자기조절력에 긍정적인 영향을 미치며, 그로 인해 키 성장에도 긍정적인 영향을 미친다는 것이 확인되었다.

• 한국항공운항학회지
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• 제29권3호
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• pp.1-14
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• 2021

This study examined the mediating role of cognitive emotion regulation in the relationship between calling and posttraumatic growth (PTG) and the moderating role of transformational leadership among Air Force pilots. A total of 215 ROK Air Force pilots participated in this study twice with an interval of 4 weeks. The results of this study were as follows. First, calling, transformational leadership, adaptive emotion regulation, and PTG showed statistically significant correlations. Second, a mediating model showed that the relationship between calling and PTG was mediated by adaptive emotion regulation. Third, the moderation effect of transformational leadership in the relationship calling on adaptive emotion regulation was found. Finally, transformational leadership also moderated the mediating effect of calling on PTG through adaptive emotion regulation was identified. Implications, limitations, and future research suggestions were discussed.

• Archives of Pharmacal Research
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• 제29권3호
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• pp.249-255
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• 2006

Ability of iron acquisition of pathogenic microorganisms functions as a virulence factor. Candida albicans, a fungal pathogen that requires iron for growth, is susceptible to growth retardation by high-affinity iron binding proteins such as transferrin. Recently, we reported that C. albicans could utilize the heme as a part of heme-containing proteins dissociated by heme oxygenase, CaHMX1. In search of another pathway that C. albicans can use to bypass the growth regulation produced by iron limitation, this present study examined utilization of non-candidal siderophores such as Desferal and rhodotorulic acid (RA) for acquisition of inorganic iron by the fungus. C. albicans secreting no siderophores was cultured in iron-free (pretreated with apotransferrin for 24 h) (culture medium). Once growth of the yeast reached stasis from iron starvation, a siderophore was added to the culture media. Results showed that cultures containing apotransferrin within a dialysis membrane recovered growth to the level of untreated controls, whereas C. albicans yeast cells in direct contact with soluble iron-free (apo) transferrin recovered growth only partially. When static growth from iron limitation was reached, the addition of siderophore-apotransferrin complex to culture medium also permitted the yeast to recover growth from apotransferrin growth regulation. All the data show that C. albicans can utilize the non-candidal siderophores for iron acquisition under transferrin regulation as can pathogenic bacteria.

• Journal of Microbiology and Biotechnology
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• 제31권9호
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• pp.1231-1240
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• 2021

Members of the genus Bacillus are known to play an important role in promoting plant growth and protecting plants against phytopathogenic microorganisms. In this study, 21 isolates of Bacillus spp. were obtained from the root micro-ecosystem of Suaeda glauca. Analysis of the 16S rRNA genes indicated that the isolates belong to the species Bacillus amyloliquefaciens, Bacillus velezensis, Bacillus subtilis, Bacillus pumilus, Bacillus aryabhattai and Brevibacterium frigoritolerans. One of the interesting findings of this study is that the four strains B1, B5, B16 and B21 are dominant in rhizosphere soil. Based on gyrA, gyrB, and rpoB gene analyses, B1, B5, and B21 were identified as B. amyloliquefaciens and B16 was identified as B. velezensis. Estimation of antifungal activity showed that the isolate B1 had a significant inhibitory effect on Fusarium verticillioides, B5 and B16 on Colletotrichum capsici (syd.) Butl, and B21 on Rhizoctonia cerealis van der Hoeven. The four strains grew well in medium with 1-10% NaCl, a pH value of 5-8, and promoted the growth of Arabidopsis thaliana. Our results indicate that these strains may be promising agents for the biocontrol and promotion of plant growth and further study of the relevant bacteria will provide a useful reference for the development of microbial resources.

• 디지털융복합연구
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• 제17권10호
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• pp.479-487
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• 2019

이 연구는 외상을 경험한 청소년 운동선수들을 대상으로 포커싱적 태도와 인지적 정서조절이 외상 후 성장에 미치는 영향력을 밝히고자 하였다. 이를 위해 S시와 K도 지역에 위치한 중, 고등학교 운동선수 215명을 대상으로 예비조사를 통해 타당성과 신뢰성이 검증된 설문지를 사용하여 포커싱적 태도와 인지적 정서조절이 외상 후 성장에 영향을 미치는지 파악한 결과, 청소년 운동선수들의 포커싱적 태도와 인지적 정서조절은 외상 후 성장에 영향을 미치며, 외상 후 성장에 영향을 미치는 요인은 긍정적재평가요인과 알아차리기, 수용, 받아들이기, 되새겨보기 및 긍정적 초점변경요인으로 영향력이 있는 것으로 나타났다. 이 연구에서 나타난 결과를 통해 외상 후 성장을 경험하기 위해서는 포커싱적 태도와 인지적 정서조절에 대한 총체적인 이해와 기전에 대해 파악하는 것이 중요하며, 이에 청소년 운동선수들의 포커싱적 태도와 인지적 정서조절에 대해 포괄적으로 개념을 파악하고 이를 통해 포커싱적 태도 수준을 제고하고 인지적 정서를 긍정적으로 조절할 수 있는 효율적인 심리적 훈련 방법의 개발이 필요할 것으로 사료된다.

• Journal of Photoscience
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• 제9권2호
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• pp.94-97
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• 2002

Light is one of the most important environmental factors that influence plant growth and development. It does not function independently but exerts its role through coordinated interactions with intrinsic developmental programs, such as hormonal regulation. One typical example is hypocotyl growth in which light signals are modulated through growth hormones. However, the underlying molecular mechanisms are largely unknown. We demonstrated that brassinosteroids play an important role in the light signal transduction in etiolated hypocotyl growth. A light-responsive Ras-like G-protein, Pra2 from pea, physically and functionally interacts with a cytochrome P450 that specifically catalyzes C-2 hydroxylation in brassinosteroid biosynthesis. The cytochrome P450 expression, along with Pra2, is induced in the dark and predominantly localized in the rapidly elongating zone of etiolated pea epicotyls. Transgenic plants with a reduced level of Pra2 exhibit a dark-specific dwarfism, which is completely rescued by brassinosteroid application. On the contrary, overexpression of the cytochrome P450 results in enhanced hypocotyl growth even in the light, which phenocopies the etiolated hypocotyl growth. It is therefore envisioned that Pra2 is a molecular switch that mediates the crosstalk between light and brassinosteroids in the etiolation process.

• 에너지공학
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• 제29권1호
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• pp.1-12
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• 2020

세계적으로 기후변화에 대응하기 위한 탄소배출 규제가 강화됨에 따라 한국도 기후변화협약의 당사국으로서 강력한 이행을 권고 받고 있다. 특히 한국은 환경규제 강화에 대한 정부와 산업계, 국민의 이해관계가 복잡한 만큼 환경규제가 경제성장과 전력소비량, 이산화탄소 배출량 감축에 어떠한 영향을 미치는지에 관한 연구는 매우 중요하다. 이에 본 논문은 한국에 있어 환경규제와 경제성장, 전력소비량, 이산화탄소 배출량에 관한 인과관계를 실증분석하였다. 이를 위해 1987 ~ 2014년 동안의 시계열 자료를 바탕으로 단위근 검정, 공적분 검정, 벡터오차수정모형(VECM)을 통한 인과성 검정을 실시하였다. 분석의 결과, 환경규제는 전력소비량과 이산화탄소 배출량 간 양방향의 장기 및 강인과관계를 가지며, 전력소비량과 단기적으로 영향을 받는 반면, 경제성장에는 영향을 미치지 못하면서 일방적으로 장기 및 강인과관계로 영향을 받는 것으로 분석되었다. 경제성장은 장기적으로 전력소비량, 이산화탄소 배출량, 환경규제에 영향을 미치면서 단기적으로 전력소비량에 단방향의 인과관계를 보였고, 이산화탄소 배출량은 양방향의 인과관계를 갖는 것으로 나타났다. 이러한 분석결과에 대응할 수 있는 정책적 시사점으로는, 환경규제가 이산화탄소 배출량 감축을 위한 기술혁신과 생산성 향상을 유발하여 경제성장에 기여할 수 있도록 하는 정책개발이 필요하다. 또한 국제 탄소배출 규제가 본격적으로 실행될 경우, 환경규제가 혁신을 통한 경제성장, 전력소비량에 따른 전원구성의 변화와 이산화탄소 배출량의 감축 등에 관련된 정책 간에 충돌을 일으키지 않고 시너지 효과를 창출하도록 하는 세심한 정책구상이 필요하다.

• BMB Reports
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• 제49권11호
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• pp.598-606
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• 2016

ARF is an alternative reading frame product of the INK4a/ARF locus, inactivated in numerous human cancers. ARF is a key regulator of cellular senescence, an irreversible cell growth arrest that suppresses tumor cell growth. It functions by sequestering MDM2 (a p53 E3 ligase) in the nucleolus, thus activating p53. Besides MDM2, ARF has numerous other interacting partners that induce either cellular senescence or apoptosis in a p53-independent manner. This further complicates the dynamics of the ARF network. Expression of ARF is frequently disrupted in human cancers, mainly due to epigenetic and transcriptional regulation. Vigorous studies on various transcription factors that either positively or negatively regulate ARF transcription have been carried out. However, recent focus on posttranslational modifications, particularly ubiquitination, indicates wider dynamic controls of ARF than previously known. In this review, we discuss the role and dynamic regulation of ARF in senescence and cancer.

• International Journal of Computer Science & Network Security
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• 제22권4호
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• pp.67-72
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• 2022

The main purpose of the study is to analyze the main aspects of state regulation of the banking business in the context of social and digital transformation. One of the key elements of the functioning of the economy of any country are banks that ensure the redistribution of financial resources and stimulate economic growth. However, the banking sector, like other activities, is dynamic and depends on the pace of development and forms of technological progress that affect the forms and types of information and digital technologies, as well as the globalization and remoteness of banking services. Accordingly, the need for effective implementation of the latest technologies becomes relevant, which will not only help increase consumer satisfaction with the banking product, but also ensure the development of the country's financial sector. As a result of the study, trends in the development of state regulation of the banking sector in the digital economy were identified.