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Combined Effects of Sanitizer Mixture and Antimicrobial Ice for Improving Microbial Quality of Salted Chinese Cabbage during Low Temperature Storage (저온 저장 중 절임배추의 미생물학적 품질 향상을 위한 혼합 살균제재와 항균성 얼음 병합처리 효과)

  • Choi, Eun Ji;Chung, Young Bae;Han, Ae Ri;Chun, Ho Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.11
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    • pp.1715-1724
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    • 2015
  • The combined effects of a sanitizer mixture solution and antimicrobial ice on the quality of salted Chinese cabbages were examined. Salted Chinese cabbages were treated with a sanitizer mixture (comprised 50 ppm aqueous $ClO_2$ and 0.5% citric acid), packed in 2% brine and antimicrobial ice, and stored for 12 days at 4 and $10^{\circ}C$. Microbiological data on the salted Chinese cabbages after washing with the sanitizer mixture indicated that the populations of total aerobic bacteria, and yeast and molds decreased by 2.20 and 1.28 log CFU/g after treatment with the sanitizer mixture. In addition, coliforms population of salted Chinese cabbage after 12 days storage at $4^{\circ}C$ in the combined mixture of the sanitizer and antimicrobial ice was 3.22 log CFU/g, which was a significantly different from that of control (5.46 log CFU/g). The combined treatment of sanitizer mixture, antimicrobial ice, and low temperature at $4^{\circ}C$ suppressed reduction of pH and elevation of titratable acidity, resulting in delaying the growth of lactic acid bacteria. Differences in salinity, hardness, and Hunter's $L^*$, $a^*$, and $b^*$ values among treatments were negligible during storage at $4^{\circ}C$. Therefore, this study suggests that a combination of sanitizer mixture, antimicrobial ice treatment, and low temperature storage could improve the microbial safety and quality of salted Chinese cabbages during storage.

Preparation and Quality Characteristics of Makgeolli Made with Black Garlic Extract and Sulgidduk (설기떡을 이용한 흑마늘 막걸리의 제조와 품질 특성)

  • Kim, Gyoung-Min;Jung, Woo-Jae;Shin, Jung-Hye;Kang, Min-Jung;Sung, Nak-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.5
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    • pp.759-766
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    • 2011
  • We conducted this study to develop a high value black garlic Makgeolli that was made of black garlic extract (BGE) and Sulgidduk. We investigated the quality characteristics of Makgeolli made with three different combinations of materials (control, Sulgidduk only; A, Sulgidduk combined with 15% BGE and water; B, Sulgidduk combined with 15% BGE instead of water). The pH of A and B were higher than the control, but the titratable acidity of A and B were lower. The sugar and alcohol contents of A and B increased during fermentation. A similar growth pattern was observed invisible cells, yeast, and lactic acid bacteria in all three Makgeolli. In A and B, the quantity of lactic acid bacteria was relatively higher than the yeast. The L value (lightness) was highest in the control, and the a value (redness) and b value (yellowness) were higher in A and B. The antioxidant properties of the three types of Makgeolli were evaluated using DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis[3-ethylbenzthiazoline-6-sulfonic acid]) radical scavenging activities. In these assays, B showed significantly higher radical scavenging activities than the other two Makgeolli.

Fermentative Characteristics of Kimchi Prepared by Addition of Different Kinds of Minor Ingredients (부재료를 달리하여 제조한 김치의 발효특성)

  • Yi, Jin-Heui;Cho, Young;Hwang, In-Kyeong
    • Korean journal of food and cookery science
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    • v.14 no.1
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    • pp.1-8
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    • 1998
  • The characteristics of Kimchi, associated with proceeding velocity of fermentation, and microbial changes, were investigated during fermentation where kimchi were prepared by the addition of different kinds of minor ingredients. In all samples, total acidity increased, and the pH and total reducing sugar content decreased as the fermentation proceeded. Redox potentials decreased from early to middle fermentation period but thereafter increased and had the lowest value at optimum ripening stage. The counts of total viable and lactic acid bacteria increased rapidly at the begining and decreased slowly. These bacterial counts of kimchi samples containing ginger, red pepper powder, and mixture of all minor ingredients were higher than those of other samples. The number of Leuconostoc mensenteroides reached the maximum at the optimum fermenting stage, Lactobacillus plantarum appeared at the time when Leu. mesenteroides reduced. All these phenomena were observed earlier in the samples containing raw red pepper and later in the samples added garlic than in other samples. It is suggested that garlic is the ingredient which improved storage capacity by prolonging fermentation period by lactic acid bacteria beneficial to less acidification, and ginger and red pepper powder are materials which gave better taste to kimchi by stimulating the growth of Leu. mesenteroids. And redox potential was an useful indicator in choosing optimum ripening time during fermentation.

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Evaluation of Soil in Planting Ground at Seashore Reclaimed Land in Incheon (인천지역 해안매립지 식재기반 평가 연구)

  • Kim, Won-Tae;Yoon, Yong-Han;Kim, Jeong-Ho
    • Journal of Environmental Science International
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    • v.20 no.11
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    • pp.1447-1455
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    • 2011
  • The purpose of this study was to analyze physicochmical characteristics of oil within planting base of Incheon coastal reclamation cities where 10 years have passed since reclamation, and to provide basic data to promote growth and development of plants by conducting evaluation based on the planting degree standard. Study targets were Cheongra, Sondo, and Yeongjong districts within Incheon Free Economic Zone, the coastal reclamation city. The analysis results of average soil characteristics of 3 districts. Soil acidity(pH) was shown in orders of Cheongra district(8.85) > Songdo district(8.70) > Yeongjong district(7.97) and electric conductivity(EC) was shown in orders of Cheongra district(4.80 dS/m) > Songdo district(1.30dS/m) > and Yeongjong district(0.07 dS/m). Organic matter content(OMC) was shown in orders of Cheongra district(5.72%) > Songdo district(2.60%) > Yeongjong district(0.59%) and available phosphate was shown in orders of Cheongra district(70.70 mg/kg) > Songdo district(23.07 mg/kg) > Yeongjong district(2.49 mg/kg). $K^+$ was shown in orders of Songdo district(0.84 cmol/kg) > Cheongra district(0.74 cmol/kg) > Yeongjong district(0.22 cmol/kg), $Ca^{++}$ was shown in orders of Cheongra district(22.08 cmol/kg) > Songdo district(9.87 cmol/kg) > Yeongjong district(4.04 cmol/kg), and $Mg^{++}$ was shown in orders of Cheongra district(1.98 cmol/kg) > Songdo district(1.22 cmol/kg) > Yeongjong district(1.12 cmol/kg). Planting base level of coastal reclaimed land can be applied with above intermediate level, soil acidity and available phosphate were all more than intermediate in 3 districts, and electric conductivity was low level in Cheongra district and more than intermediate level in Songdo and Yeongjong districts. Organic matters content was more than intermediate level in Cheongra district and low level in Songdo and Yeongjong district. $K^+$ among exchangeable ions was intermediate level in Cheongra and Songdo districut and low in Yeongjong district. $Ca^{++}$ and $Mg^{++}$ both were more than imtermidiate levels in 3 districts.

TWO COLORIMETRIC ASSAYS VERIFY THAT CALCIUM SULFATE PROMOTES PROLIFERATING ACTIVITY OF HUMAN GINGIVAL FIBROBLASTS

  • Chae, Min;Kim, Su-Yeon;Kim, Soo-Yeon;Lee, Suk-Won
    • The Journal of Korean Academy of Prosthodontics
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    • v.45 no.3
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    • pp.382-388
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    • 2007
  • Statement of problem. The role of calcium sulfate in stimulating the growth of gingival soft tissue has been reported in few studies. Such a unique property of calcium sulfate could serve as a trouble-solving broker in compensating for the lack of soft tissues in various oral surgeries. Purpose. The purpose of this study was to compare the proliferating activities of human gingival fibroblasts seeded on various bone graft barrier materials of calcium sulfate, collagen, and polytetrafluorethylene (PTFE). Material and methods. Two calcium sulfates ($CAPSET^{(R)}$. and $CalForma^{(R)}$, Lifecore Biomedical Inc., St. Paul, Minnesota, USA), a resorbable natural collagen ($Bio-Gide^{(R)}$, Geistlich Pharma Ag., Wolhusen, Switzerland), and a non-resorbable PTFE ($TefGen-FD^{(R)}$, Lifecore Biomedical Inc., St. Paul, Minnesota, USA) served as the human gingival fibroblasts' substrates and comprised the four experimental groups, whereas the untreated floors of culture plastics were used in the control group, in this study. Cells were trypsinized, seeded, and incubated for 48 h. The proliferating activities of fibroblasts were determined by XTT and SRB assay and absorbance (optical density, OD) was measured. One-way ANOVA was used to analyze the differences in the mean OD values between the groups of CAPSET, CalForma, Bio-Gide, TefGen, and the control (p<0.05). Results. From the XTT assay, the mean OD value of the control group, the highest, was significantly greater than that of any of the four experimental groups followed by CalForma, CAPSET, TefGen, and Bio-Gide. Further, the mean OD value of CalForma, was significantly greater compared to that of Bio-Gide. From the SRB assay, Calforma showed the highest mean OD value, which was significantly greater than that of any other groups, followed by the control, CAPSET, Bio-Gide, and TefGen. The mean OD values of both the control and CAPSET were significantly greater compared to that of TefGen (p<0.05). Conclusion. Assessment of the viability and proliferation of cultured fibroblasts seeded and incubated for 48 h on various barrier-material substrates using XTT and SRB assay showed that calcium sulfate $CalForma^{(R)}$ promotes the proliferating activity of human gingival fibroblasts.

Damage Conditions and Assessment for Cut Slope Structures due to Acid Rock Drainage (산성암반배수에 의한 절취사면 구조물의 피해 현황과 평가)

  • Lee Gyoo Ho;Kim Jae Gon;Park Sam-Gyu;Lee Jin-Soo;Chon Chul-Min;Kim Tack Hyun
    • Journal of the Korean Geotechnical Society
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    • v.21 no.5
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    • pp.83-92
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    • 2005
  • The aim of this study was to investigate damage conditions of cut slope structures due to acid rock drainage (ARB) and to assess the acid production potential of various rocks. Acid rock drainage is produced by the oxidation of sulfide minerals contained in coal mine zone and mineralization belt of Pyeongan supergroup and Ogcheon group, pyrite-bearing andesite, and Tertiary acid sulfate soils in Korea. Most of cut slopes producing ARB have been treated with shotcrete to reduce ARD. According to the field observations, ARD had an adverse effect on slope structures. The corrosion of shotcrete, anchors and rock bolts and the bad germination and growth diseases of covering plants due to ARD were observed in the field. The concentration of heavy metals and pH of ARD from cut slope exceeded the environmental standard, indicating a high potential of environmental pollution of surrounding soil, surface water and ground water by the ARD. According to acid base accounting (ABA) of the studied samples, hydrothermally altered volcanic rocks, tuffs, coaly shales, tailings of metallic mine had a relatively high potential of acid production but gneiss and granite had no or less acid production potential. It is expected that the number of cut slopes will increase hereafter considering the present construction trend. In order to reduce the adverse effect of ARD in construction sites, we need to secure the data base for potential ARD producing area and to develop the ARD reduction technologies suitable.

A new natural habitat of Abeliophyllum distichum Nakai (미선나무(Abeliophyllum distichum Nakai)의 새로운 자생지 보고)

  • Kim, Dong-Kap;Kim, Joo-Hwan
    • Korean Journal of Plant Taxonomy
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    • v.38 no.4
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    • pp.573-582
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    • 2008
  • We report a newly found natural habitat of Abeliophyllum distichum in mountainous slope range of Yeongdong-gun, Chungbuk Province. Abeliophyllum distichum Nakai is one of the Korean monotypic endemic species. Natural growth habitats of this species have been recorded from seven sites up to now, and all of the natural habitats are located in middle (Chungbuk Prov.) and middle west (Jeonbuk Prov.) parts of South Korea. Among the previously recorded seven natural habitats, six sites have been designated as Korean national monuments and protected with in situ conservation. New natural habitat of A. distichum is located on northwest slope of stiff hillock area beside the small stream, Seolgye-ri, Yeongdong-eup, Yeongdong-gun, Chungbuk Province. Total growing area is nearly $3,000 m^2$. It is 10-25 cm in soil depth and pH 5.0-6.5 in soil acidity in that area. And many of A. distichum are clustered with 2-5 individuals extended by stoloniferous asexual reproduction. And the total numbers of A. distichum are about 700 individuals with only typical white flowers, and the ratio between pin type and thrum type is 37% and 63%, respectively. The huge population of A. distichum is growing with Quercus mongolica-Fraxinus rhynchophylla association in a mixed forest, and it shows high affinity with Stephanandra incisa, Ligustrum obtusifolium, Euonymus alatus for. ciliatodentatus, and Smilax sieboldi.

A Study on High Strength Nitrogen Wastewater Treatment and Sludge Granulation Characteristics in a Pilot-scale Air-lift Sequencing Batch Reactor (파일럿 규모의 공기 유동 연속회분반응기에서 고농도 질소제거 및 슬러지 그래뉼화 특성 연구)

  • Lee, Soochul;Kim, Dong-Jin
    • Clean Technology
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    • v.18 no.4
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    • pp.398-403
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    • 2012
  • Selective nitrification and granulation have been carried out in a pilot scale air-lift sequencing batch reactor (SBR) for stable and economical nitrogen removal from wastewater. The SBR showed about 100% nitrification efficiency up to 1.0 kg ${NH_4}^+-N/m^3{\cdot}d$, about 90% efficiency at 1.0-2.0 kg ${NH_4}^+-N/m^3{\cdot}d$, and it was less than 90% when the load was higher than 2.0 kg ${NH_4}^+-N/m^3{\cdot}d$. Nitrite accumulation was induced by selective inhibition of nitrite oxidizing bacteria by free ammonia inhibition and dissolved oxygen limitation. For the purpose, high nitrite ratio (> 0.95) was obtained by keeping the pH higher than 8.0 and dissolved oxygen lower than 1.5 mg/L. In addition, sludge granulation was achieved by keeping reactor settling time to 5 minutes to wash out poor settling sludge and to promote the growth of granulation sludge. The operation accelerated sludge granulation and the sludge volume index (SVI) decreased and stably maintained to less than 75 in 60 days.

Effect of Choi-cha on Fermentation Characteristics and Sensory Quality of Chungkookjang (Korean Fermented Soybean) (초의차 첨가가 청국장의 발효특성과 관능적 품질에 미치는 영향)

  • Lee, Hyun-Joo;Kim, Sun-Im;Park, Jin-Gyu;Park, Jae-Nam;Han, In-Jun;Song, Beom-Seok;Kim, Jae-Hun;Byun, Myung-Woo;Lee, Ju-Woon
    • Food Science and Preservation
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    • v.15 no.1
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    • pp.144-149
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    • 2008
  • Physiological, sensory and microbiological evaluations were conducted during fermentation of Chungkookjang. with Choi-cha (non-fermented green tea) powder in efforts to improve the sensory quality of Chungkookjang. Growth of fermentative microorganisms in Chungkookjang was inhibited by Choi-cha; the pH and VBN value decreased as the concentration of Choi-cha increased. Brightness (L) and redness (a) of Chungkookjang fermented with Choi-cha were significantly decreased, resulting in the appearance of all samples fermented with Choi-cha being less attractive than was the control. The sensory quality of Chungkookjang was significantly increased when Choi-cha was added up to 3%, (w/v), and off-odor was reduced. The antioxidative property of Chungkookjang fermented with 3% (w/v) Choi-cha was significantly higher than that of the control. This result indicated that the sensory quality of Chungkookjang was improved by fermentation with 3% (w/v) of Choi-cha powder.

Development in Planting Porous Block for Revegetation (녹화용 다공질 식재 블럭의 개발)

  • Ahn, Young-Hee;Choi, Kyoung-Young
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.5 no.4
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    • pp.1-9
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    • 2002
  • This study is carried out to make the environmentally affinitive porous planting block for revegetation and to make a effective program for greening plans. The summary is shown below. 1. In order to get stronger intensity and distribute proper porosity in the block for planting, the cements mixed with fine soil were used and the finer in soil grains gives the stronger in intensity of the cements. Use of the furnace slag cements instead of the portland cements showed relatively stronger in intensity of the block. The intensity of the block became stronger when the mixed ratio of the cements to soil is 5 : 1, but the pore space ratio was lower. The percolate pH of the portland cements after one month of treatment was 13.1 but the percolate pH of the furnace slag cements was shown lower. To mold proper porous planting blocks, the proper combination of additives such as the dehydrating agent, elastic agent and adhesives into the mixture of cements and soil gives better effectives. 2. After molding the porous planting blocks, it gave a better result when the grains of the filler made of peat moss, upland soil and compound fertilizer were smaller than 2 mm in size. Shaking of the filling materials also gave the better result, but it took more time and cost much more. Therefore, it was better when the filling materials were mixed with water first then flew down for stuffing. 3. It was necessary to cover with soil after seeding or planting on the porous planting blocks. The proper thickness of the soil to help root development and keep moisture is about 3~5 cm. 4. The plants for planting on the porous planting block were required stronger in the growth condition of their roots and their environmental adaptability. The average germination percentage and rate of Platycodon grandiflorum on the porous planting block were 88.8% and 85% accordingly and their rate is very uniform. The germination rates of Dianthus superbus var. longicalycinus and Taraxacum officinale were more than 50%. These grass species, Chelidonium majus var. asiaticum, Lysimachia mauritiana and Scabiosa mansenensis were the suggested biennial grasses in the planting area where exchanging of the seedling or nursery plants was not necessary because their germination rates were 59.3, 45.6 and 40.3% accordingly. Viola kapsanensis, Chrysanthemum sp., Taraxacum sp. and Iris ensata var. spontanea are the grass species that could be used by seeding for greening. However, the germination rate of Solidago virga-aurea var. asiatica, Aster scaber and Lythrum anceps were lower than 10%. The coverage ratio of Ixeris stolonifera is more than 80% after 60 days seeding and the root length of most of species are more than 10 cm except Iris ensata var. spontanea and Platycodon grandiflorum because their root developed thicker than other species.