• 제목/요약/키워드: growth pH

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The Suitable Condition for Mycelial Growth of Volvariella volvacea strains and Selection of the Superior strain (풀버섯의 균사배양 적합조건 및 우량균주 선발)

  • Jang, Myoung-Jun;Lee, Han-Bum;Kim, Jeong-Han;Lee, Yun-Hae;Ju, Young-Cheol
    • The Korean Journal of Mycology
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    • 제37권2호
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    • pp.173-180
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    • 2009
  • This study was carried out to obtain basic data on mycelial growth characteristics for an artificial cultivation of Volvariella volvacea. Twelve URP primers were used to assess genetic relationship of V. volvacea and its result was divided into two groups. But there was nothing different in morphological characteristics in V. volvacea. Among five kinds of mushroom media, MCM medium was selected as the favorable culture medium. The optimal range of temperature and pH for mycelial growth on V. volvacea were 35oC and pH 6~8, respectively. Carbon sources had not an effect on 10 strains of V. volvacea, and nitrogen source for the optimum mycelial growth was yeast extract. Also, we selected GMVV 79004 as a superior strain of 10 strains V. volvacea based on the mycelial growth and yield.

A study on the Mycelial Growth of Lentinus lepideus in Liquid Culture (액체배양에서 잣버섯 균사체 배양에 관한 연구)

  • 신성의;차월석;강시형
    • Journal of Life Science
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    • 제13권4호
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    • pp.492-497
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    • 2003
  • This study was carried out to get the basic data for the mycelial growth of Lentinus lepideus in liquid culture. The optimal temperature and initial pH of mycelial growth of Lentinus lepideus were $25^{\circ}C$ and pH 5.5, respectively. The optimal medium was YMG medium. Among the carbon sources tested, glucose was effected to the mycelial growth and optimal glucose concentration was 4% (w/v). As nitrogen sources, malt extract and yeast extract appeared to be favorable and optimal malt extract and yeast extract [ratio (w/w) of 1:1] concentration was 1.5% (w/v).

Growth Inhibition of Cucumber by Absorbing Excess Al at Low Soil pH (강한 산성토양에서 Al의 과잉 흡수에 의한 오이 생육장해 양상)

  • Kim, Yoo-Hak;Kim, Myung Sook;Kang, Seong Soo;Lee, Hyeong Yong
    • Korean Journal of Soil Science and Fertilizer
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    • 제45권6호
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    • pp.925-927
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    • 2012
  • On-site diagnosis applied to soil having a symptom of yellowing and whitening in cucumber leaf and stem. Soil pH determined 4.2 by methods of on-site analysis and 4.5 by soil test analysis. High aluminum in soil solution extracted with water saturation was detected. Leaf and stem tissue were abundant in Al content but not in Ca. Also, N content of leaf and stem was low compared to normal N ranges. This symptom of cucumber assumed to be from the Al and nitrous acid gas toxicity by low soil pH and Eh. Conclusionally, symptom in leaf and stem of cucumber was alleviated and cucumber normally recovered during cultivation period by applying calcium hydroxide solution to correct soil pH up to 6.5. These results showed that low soil pH resulted in aluminum toxicity and N deficiency to plant growth in on-site farming.

Effect of Temperature and pH on the Biosorption of Heavy Metals by Exophiala sp. (Exophiala sp.의 중금속 흡착에 미치는 온도 및 pH의 영향)

  • Lim, Joung-Soo;Lee, So-Jin;Lee, Eun-Young
    • Microbiology and Biotechnology Letters
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    • 제36권2호
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    • pp.165-172
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    • 2008
  • To find the optimum growth and metal removal condition of isolated strain LH2, effects of the environmental factors such as medium pH, growth temperature, and metal concentrations were investigated. Based on the 18S rDNA analysis, the isolated strain was identified to Exophiala sp. with 100% homology. Isolated strain Exophiala sp. LH2 showed maximum removal efficiency of metals at the shaking conditions of pH 7 and $25^{\circ}C$. When the concentration of metal was under 200ppm, the specific metal removal velocity at pH 7 increased from 0.01 to 4.43 mg-metal $L^{-1}{\cdot}d^{-1}{\cdot}mg{\cdot}DCW^{-1}$ as the concentration of metal increased from 10 ppm to 200 ppm. When 200 ppm of each metal was contained in the culture medium adjusted with pH 7, metal removal efficiencies Cr, Cu, Ni, Pb and Zn were 99.28%, 97.67%, 91.94%, 99.77%, 99.61%, respectively.

Effect on the Growth Condition of Chionanthus retusa, Roadside Tree in Cheongyechon (청계천 이팝나무 가로수 생육환경이 성장에 미치는 영향)

  • Yoon, Sowon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • 제11권3호
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    • pp.129-138
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    • 2008
  • This study was carried out to examine the growth condition of Chionanthus retusa, roadside tree in Cheongyechon (CGC) and to investigate the relationship soil characteristics and tree vitality and chlorophyll contents of it. Growth condition of tree (condition of flower, leaves and branch, % of flowering, height, diameter at breast height, width, vitality and chlorophyll contents) and physiochemical relation item (pH, organic matter, K, Mg, Na, Ca, P) were investigated. The result are as follows : 1. The growth condition of flower, leaves and branch in the left side of CGC is better than the right side since the quantity of sunshine of left side of CGC is much more than the right side. 2. The average pH was alkaline. P and organic contents were much lower than the standards. 3. Tree vitality and chlorophyll contents were bad where were high user density and high buildings, such as 1, 2 area near Jong-gak and jongro 3 ga. Among the physiochemical factors of soil which affect tree vitality, K and P were found to be the main factors. Therefore, in order to improve the growth environment of roadside tree in CGC, it is needed to do periodical soil fertilizing and improve physical characteristics of soil such as, permeability and porosity by soil conditioner.

Identification and characteristics of Lactic Acid Bacteria Isolated from Nuruk (누룩으로부터 젖산세균의 분리 및 특성)

  • 이정훈
    • KSBB Journal
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    • 제15권4호
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    • pp.359-365
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    • 2000
  • Three lactic acid bacteria (C-1 K-3 and T-1 strain) were isolated from Nuruk and characterized subsequently. They were useful strains for production of lactic acid and their growth was inhibited at 10% ethanol pH 4 These strains were identified as lactococcus lactis subsp. lactis NR C-1 Leuconostoc mesenteroides subsp. mesenterides NR K-3 and pediococcus pentosaceus NR T-1 respectively by morphological physiological and biochemical characterization Lac lactis subsp lactis NR C-1 showed the highest lactic acid productivity. Leu measenteroides subsp mesenteroides NR K-3 showed stable lactic acid productivity and its growth was inhibited at pH 4. P pentosaceus NR T-1 had lower lactic acid productivity than the other two bacteria but it could not grow at 10% ethanol pH 4 The lactic acid productivity of these three strains in MRS broth were higher than that in Skim milk media the optimum pH and temperature for the lactic acid production of the three strains were 30-32$^{\circ}C$ and pH 6.0∼6.8 Glucose was the optimal carbon souorce for the lactic acid production. In terms of antagonism lac lactis subsp lactis NR c-1 showed somewhat inhibitory efects against some Gram positive rod and cocci such as Lactobacillus brevis and Streptococcus mitis. And Leu mesenteroides subsp mesenteroides NR K-3 showed the inhibitory effects against Streptococcus mitis but P. pentosaceus NR T-1 didn't show any inhibitory effects against tested strains.

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The Study on Decomposition of Metal-working Fluids Against Microbes (미생물을 이용한 절삭유제의 부패성능 평가에 관한 연구)

  • Kim, Young-Wun;Hong, Kwang Min;Chung, Kunwo;Park, Chan-Jo
    • Korean Chemical Engineering Research
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    • 제44권4호
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    • pp.350-355
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    • 2006
  • Growth curves of microbes were examined to evaluate decomposition of metal-working fluids and decomposition properties of metal-working fluids were experimented using controled microbes such as E. coli and K. pnemoniae. According to the results of growth curve of microbes, the growth period depended on species of microbes, 2 h of E. coli, 3 h of K. pneumoniae, 4 h of P. aeruginosa and 3 h of P. oleovarans after incubation. The colony count of E. coli and K. pneumoniae controled to OD of 0.5 ranged from $4.4{\sim}10.0{\times}10^5CFU/mL$ and $1.8{\sim}9.5{\times}10^7CFU/mL$, respectively. The decomposition of metal-working fluids was excellently progressed in the range of pH 6~8 than below pH 4 and above pH 10. In the case of controled fluids to pH 6~8, the decomposition of the fluid containing ester group was more accelerated than that of the fluid containing ethylene glycol.

Effect of Different pH Processes on Branched β-1,3-Glucan Production from Submerged Culture of Ganoderma lucidum (영지 (G. lucidum)의 액체배양에 의한 β-1,3-Glucan 생산에 미치는 서로 다른 pH Process의 영향)

  • Lee, Shin-Yaung;Lee, Kyu-Min
    • Journal of Industrial Technology
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    • 제20권A호
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    • pp.45-50
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    • 2000
  • A submerged cultivation of Ganoderma lucidum was carried out in an air-lift fermenter system, and the effects of different pH processes on extracellular branched ${\beta}$-1,3-glucan(EPS) production and mycelial growth(MDW) were investigated. The controlled pH process improved the production of branched ${\beta}$-1,3-glucan and biomass in comparison to the uncontrolled pH process. However, the maximum production of branched ${\beta}$-1,3-glucan were obtained by the bi-staged pH process. From these results, we confirmed that the bi-staged pH process was the most effective for improving the production of branched ${\beta}$-1,3-glucan from submerged culture of G. lucidum.

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Effect of Maillard Reaction Products on Growth of Bacillus sp. (Maillard 반응생성물이 Bacillus sp.의 생육특성에 미치는 영향)

  • Lee, Gee-Dong;Kim, Jeong-Sook;Kwon, Joong-Ho
    • Korean Journal of Food Science and Technology
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    • 제29권2호
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    • pp.309-313
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    • 1997
  • Maillard reaction products (MRPs) added into a culture and the resultant bacterial growth were investigated using response surface methodology. The coefficients of determination $(R^{2})$ of response surface regression equations for bacteria were 0.9544 and 0.9578 in Bacillus subtilis and Bacillus natto, respectively. The MRPs produced at higher reaction temperature and for longer reaction time showed greater antimicrobial effect for Bacillus subtilis. Especially, the MRPs produced at temperature above $150^{\circ}C$ for 8 to 12 hrs showed the strongest antimicrobial effect. The MRPs produced at lower reaction temperature and for shorter reaction time showed greater microbial growth effect for Bacillus natto, but those produced at the reaction temperature higher than $160^{\circ}C$ showed the greatest antimicrobial effect. In the ridge analysis, the growth of Bacillus subtilis was the most significantly inhibited in the presence of MRPs prepared at $159.10^{\circ}C$ and pH 12.21 for 9.67 hrs, and the growth of Bacillus natto was the most significantly inhibited in the presence of MRPs prepared at $169.94^{\circ}C$ and pH 9.66 for 9.22 hrs.

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Growth Performance and Economic Evaluation of Insect Feed Powder-Fed Ducks (곤충분말 사료를 오리사료에 첨가 시 생산성과 경제성에 대한 평가)

  • Jang, Woo-Whan;Chung, Tae-Ho;Choi, In-Hag
    • Journal of Environmental Science International
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    • 제28권8호
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    • pp.709-712
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    • 2019
  • The effect of insect feed powder on the growth performance and economic evaluation of ducks was investigated. One hundred and twenty-old Pekin ducks were randomly assigned to two dietary treatments (0% and 1% Hermetia illucens powder) with three replicates of 20 birds each for 14-42 d. No significant (p>0.05) difference in growth performance between the control and 1% H. illucens powder was observed, except the feed conversion ratio (p<0.05), for the experimental period. The treatments with 1% H. illucens powder improved the economic indicators in comparison with the controls. Therefore, a diet supplemented with 1% H. illucens powder could significantly improve the feed conversion ratios and increase the economic indicators.