The influence of cellulosic and hemicellulosic substrates on the production of cellulase and xylanase complexes in Aspergillus niger was investigated. The culture conditions with different substrates exhibited profound effects on the level of endoglucanase (CMCase), ${\beta}-glucosidase$, endoxylanase and ${\beta}-xylosidase$, and on their isozyme patterns. However, intracellular and extracellular isozyme patterns of cellulase and xylanase complexes were qualitatively identical and appeared to be simultaneous in the early growth phase. Prolonged incubation led to the increase in the concentrations of isozymes with a little changes in the relative proportions of those isozymes. These results suggest that the biosynthesis of cellulase and xylanase complexes in A. niger is coordinately regulated at the level of induction. Moreover, multiple forms of extracellular cellulase and xylanase complexes seem to be the outcome of specific gene expression and should not be considered solely as the consequence of post-secretional modification of synthesized enzymes.
The effect of additives on the castability of the AC2BS aluminum alloy, which contains 35% recycled scrap, was investigated. For the wide utilization of the recycled scrap AC2BS aluminum alloy, the research results were compared to those with the AC2B virgin alloy, which is typical Al-Si-Cu alloy system. It was confirmed that the addition of Al-5%Ti-1%B increased the ${\alpha}$-Al nucleation temperature during solidification and decreased the grain size through cooling curve and microstructural observations of the recycled alloy. It was also found that an addition of Al-10%Sr decreased the eutectic Si growth temperature during the solidification process and modified the shape of the eutectic Si of the recycled alloy. The characteristics of fluidity, shrinkage and solidification crack strength were evaluated. For the AC2BS aluminum alloy containing 35% recycled scrap, both ${\alpha}$-Al grain refinement due to Ti-B and eutectic Si modifications due to Sr contributed to the improvement of the fluidity. The macro- shrinkage ratio increased with additions of both Al-10%Sr and Al-5%Ti-1%B and the micro-shrinkage ratio increased with the addition of Al-10%Sr but decreased with the addition of Al-5%Ti-1%B. The casting characteristics of TiB and Sr-treated AC2BS aluminum alloy containing 35% recycled scrap are similar to those of AC2B virgin alloy. The improvement of the solidification crack strength of the AC2BS aluminum alloy was possible by the reduction of the grain boundary the stress concentration through the enhancement by both ${\bullet}{\cdot}$-Al refinement and eutectic Si modification. More extensive use of the AC2BS aluminum alloy containing 35% recycled scrap can be expected in the future.
Hirata, T.;Tsutsui, C.;Yokoi, Y.;Sakatani, Y.;Mori, A.;Horii, A.;Yamamoto, T.;Taguchi, A.
Proceedings of the Korean Vacuum Society Conference
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2010.02a
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pp.44-45
/
2010
We are currently conducting studies on culturing and biocompatibility assessment of various cells such as neural stem cells and induced pluripotent stem cells(IPS cells) on carbon nanotube (CNT), on nerve regeneration electrodes, and on silicon wafers with a focus on developing nerve integrated CNT based bio devices for interfacing with living organisms, in order to develop brain-machine interfaces (BMI). In addition, we are carried out the chemical modification of carbon nanotube (mainly SWCNTs)-based bio-nanosensors by the plasma ion irradiation (plasma activation) method, and provide a characteristic evaluation of a bio-nanosensor using bovine serum albumin (BSA)/anti-BSA binding and oligonucleotide hybridization. On the other hand, the researches in the case of "novel plasma" have been widely conducted in the fields of chemistry, solid physics, and nanomaterial science. From the above-mentioned background, we are conducting basic experiments on direct irradiation of body tissues and cells using a micro-spot atmospheric pressure plasma source. The device is a coaxial structure having a tungsten wire installed inside a glass capillary, and a grounded ring electrode wrapped on the outside. The conditions of plasma generation are as follows: applied voltage: 5-9 kV, frequency: 1-3 kHz, helium (He) gas flow: 1-1.5 L/min, and plasma irradiation time: 1-300 sec. The experiment was conducted by preparing a culture medium containing mouse fibroblasts (NIH3T3) on a culture dish. A culture dish irradiated with plasma was introduced into a $CO_2$-incubator. The small animals used in the experiment involving plasma irradiation into living tissue were rat, rabbit, and pick and are deeply anesthetized with the gas anesthesia. According to the dependency of cell numbers against the plasma irradiation time, when only He gas was flowed, the growth of cells was inhibited as the floatation of cells caused by gas agitation inside the culture was promoted. On the other hand, there was no floatation of cells and healthy growth was observed when plasma was irradiated. Furthermore, in an experiment testing the effects of plasma irradiation on rats that were artificially given burn wounds, no evidence of electric shock injuries was found in the irradiated areas. In fact, the observed evidence of healing and improvements of the burn wounds suggested the presence of healing effects due to the growth factors in the tissues. Therefore, it appears that the interaction due to ion/radicalcollisions causes a substantial effect on the proliferation of growth factors such as epidermal growth factor (EGF), nerve growth factor (NGF), and transforming growth factor (TGF) that are present in the cells.
It has long been known that nutritional and environmental influences during the early developmental period affect the biological mechanisms which determine animal metabolism. This phenomenon, termed 'metabolic imprinting', can cause subtle but long-lasting responses to prenatal and postnatal nutrition and even be passed onto the next generation. A large amount of research data shows that nutrient availability, in terms of quantity as well as quality, during the early developing stages can decrease the number of newborn piglets and their body weight and increase their susceptibility to death before weaning. However, investigation of potential mechanisms of 'the metabolic imprinting' effect have been scant. Therefore, it remains unknown which factors are responsible for embryonic and early postnatal nutrition and which factors are major determinants of body weight and number of new born piglets. Intrauterine undernutrition, for example, was studied using a rat model providing dams 50% restricted nutrients during pregnancy and the results showed significant decreases in birth weight of newborns. This response may be a characteristic of a subset of modulations in embryonic development which is caused by the metabolic imprinting. Underlying mechanisms of intrauterine undernutrition and growth retardation can be explained in part by epigenetics. Epigenetics modulate animal phenotypes without changes in DNA sequences. Epigenetic modifications include DNA methylation, chromatin modification and small non-coding RNA-associated gene silencing. Precise mechanisms must be identified at the morphologic, cellular, and molecular levels by using interdisciplinary nutrigenomics approaches to increase pig production. Experimental approaches for explaining these potential mechanisms will be discussed in this review.
A modified method for the analysis of short fatigue crack growth has been presented, and calculations based upon the modified method are compared with experimental results for S45C carbon steel. It is also shown that the modified method is in good agreement with experimental data. The proposed equation for the fatigue crack growth rates includes a material constant which relates the threshold level to the endurance limit, a correction for elastic-plastic behaviour and a means for dealing with the effects of crack closure. In this study one of the modifications is to substitute the Forman' s elastic expression of the stress intensity factor range into the geometrical factor The other is a consideration of the bending effect which is developed from the moment caused by the eccentric cross sectional geometry as the crack grows. Thus, this method is useful for residual life prediction of the mechanical structures as well as the welding structures.
PURPOSE. The purpose of this study was to compare the removal torques of a chemically modified SLActive implant and a blasted, laser-treated (BLT) implant, which were soaked in saline for 2 weeks after their surface modifications. The removal torques of the two implants were measured 4 weeks after their implantation into the bone defect area in rabbit tibias with concentrated growth factor (CGF) application. MATERIALS AND METHODS. To make artificial bone defects in the cortical layers of both tibias, an 8-mm diameter trephine bur was used. Then, prepared CGF was applied to the bony defect of the left tibia, and the bony defect of the right tibia was left unfilled. Four weeks later, the surgical sites of 16 rabbits were re-exposed. For 8 rabbits, the SLActive implants (Straumann, Switzerland) were inserted in the left tibia, and the BLT implants (CSM implant, Daegu, Korea) were inserted in the right tibia. For other rabbits, the BLT implants were inserted in the left tibia, and the SLActive implants were inserted in the right. Four weeks afger the insertion, torque removal was measured from 4 rabbits exterminated via $CO_2$ inhalation. RESULTS. No significant difference was observed between removal torques of the BLT implant and the SLActive implant (P>.05). CONCLUSION. It was found that BLT surface modification exhibited excellent osseointegration. In addition, CGF application did not affect the insertion and removal torque of the implants.
Kim, Jihee;Chandra, Prakash;Yang, Jiyoon;Rhee, Seog Woo
Bulletin of the Korean Chemical Society
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v.34
no.12
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pp.3715-3721
/
2013
In this study, the behavior of cells on the modified surface, and the correlation between the modified substrates and the response of cells is described. A close-packed layer of nano-sized silica beads was prepared on a coverslip, and the adhesion, proliferation, and migration of BALB/3T3 fibroblast cells on the silica layer was monitered. The 550 nm silica beads were synthesized by the hydrolysis and condensation reaction of tetraethylorthosilicate in basic solution. The amine groups were introduced onto the surfaces of silica particles by treatment with 3-aminopropyltrimethoxysilane. The close-packed layer of silica beads on the coverslip was obtained by the reaction of the amine-functionalized silica beads and the (3-triethoxysilyl)propylsuccinic anhydride treated coverslip. BALB/3T3 fibroblast cells were loaded on bare glass, APTMS coated glass, and silica bead coated glass with the same initial cell density, and the migration and proliferation of cells on the substrates was investigated. The cells were fixed and stained with antibodies in order to analyze the changes in the actin filaments and nuclei after culture on the different surfaces. The motility of cells on the silica bead coated glass was greater than that of the cells cultured on the control substrate. The growth rate of cells on the silica bead coated glass was slower than that of the control. Because the close-packed layer of silica beads gave an embossed surface, the adhesion of cells was very weak compared to the smooth surfaces. These results indicate that the adhesion of cells on the substrates is very important, and the actin filaments might play key roles in the migration and proliferation of cells. The nuclei of the cells were shrunk on the weakly adhered surfaces, and the S1 stage in which DNA is duplicated in the cell dividing processes might be retarded. As a result, the rate of proliferation of cells was decreased compared to the smooth surface of the control. In conclusion, the results described here are very important in the understanding of the interaction between implanted materials and biosystems.
Kim, Ki-Hyun;Kim, Kwang-Sik;Kim, Jo-Eun;Seol, Kuk-Hwan;Kim, Young-Hwa
Korean Journal of Agricultural Science
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v.42
no.4
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pp.389-396
/
2015
This study was performed to investigate the opinions of various related-industries on ban of antibiotics growth promoters (AGPs) in commercial mixed feed. The answers on a total of 21 questions were summarized by response number and percentage. 93% of those surveyed were in agreement of a ban of dietary AGPs. The agreement reasons were the livestock safety (61.5%), the reduction of antibiotic use (23.1%), and decrease of antibiotic-resistant bacteria (11.5%). The negative effects expected by the ban of AGPs were poor growth performance (44.2%), elevated disease emergence (31.4%), increasing the feed cost (18.6%), and quality degradation of livestock (5.8%). As the efficient plans for decline of AGPs use, the feeding environment improvement was the highest with 43%, and farmer training and the consolidation inspection of residual substance on antibiotics in livestock product was 27.9% and 22.1%, respectively. 46.5% of respondent are considering the modification of feed spec and 39.5% of those surveyed have staged a modified feed spec. In conclusion, livestock related-industries approve a ban of AGPs, and they assert that the policy support, improvement of management and environment in the farm, providing technology from related-industries are multiply essential for a stable settlement of a ban policy of AGPs.
The Korean Population Control Program has been implementing under the jurisdiction of the Ministry of Health and Social Affairs through an existing network of health centers. This arrangement was successful in bringing population growth down to targeted level by the end of the Fourth Five Year Economic Planning, 1981. It is expected, however, that future goal will be harder to reach due to difficulty of reducing traditional family size norms further and to the projected increasing the number of eligible couples as the past Korean war baby boom generation enters the reproductive activity in the next few years. The recognition of the need for modification of population policy is increasing. The 1980 census shows that the total number of population reached approximately 38.5 million with 1.57 per cent of the growth rate. It was projected that the size of Korean Population will reach around 42 million and 51 million in 1986 and 2000 respectively. Furthermore, there is some argument as to whether decline in the birth rate in Korea is too slow to meet government target. Hence, a new development of population policy and greatly increased amount of effort will be needed in order to achieve Zero Population Growth Rate before the year 2050. The development of future national population policy and its related area are recommended as follow: 1. It is highly recommended that the population planning law governing both vital events of birth and death and population migration should be legislated. 2. The National Population Policy Council, Chaired by Deputy Prime Minister should be activated to implement and coordinate population program within ministries. 3. Responsible organization of population and family planning program should be established as a Bureau unit at central government level. 4. For the improvement of national vital registration, an existing system should be studied and developed.
Journal of the Korean Society of Environmental Restoration Technology
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v.23
no.1
/
pp.15-36
/
2020
The purpose of this study is to provide basic data for the establishment of the management plan of Seoullo 7017 after analyzing the characteristics of immigration plants in addition to those introduced plants. The taxa number of plants appeared as 276 taxa including 82 families, 161 genera, 246 species, 1 subspecies, 22 varieties and 7 forms. Among them, immigration plants appeared 79 classification groups, including 32 families, 65 genera, 77 species and 2 varieties. Invasive alien plants appeared as 22 classification groups in 79 classification groups of immigration plants. The ratio of America origin plants, second period(1932~1960) in introduction time and the wide diffusion species is high. In June, the growth and occurrence time of the immigration plants was the highest by monthly the characteristics of the growth of the immigration plants, the immigration plants appeared mostly. In case of dormancy form among life-form, the therophytes(th), in the propagation form-radicoid form, the R5(monophyte), in the propagation form-disseminule form, the gravity D4(having no special modification for dissemination), and in the growth form, the erect form(e) was high. The various immigration plants appeared in green areas covered by Zoysia japonica and low Hedera helix densities. This study is the first flora study since Seoullo 7017 was opened in 2017. And so continuous flora monitoring is required for Seoullo 7017.
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