• 식물조직배양학회지
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• 제28권6호
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• pp.305-310
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• 2001

본 실험은 지치 (Lithospermum erythrorhizon)의 뿌리조직으로부터 유도한 캘러스에 ${\gamma}$선을 조사하여 배양기간과 배지조성 및 명.암배양의 조건에 따라 ${\gamma}$선 조사가 캘러스 생장촉진 효과가 있는지를 검토하고자 수행하였다. 지치의 캘러스 생장은 전체적으로 암배양에서보다 명배양에서 효과적이었다. r선 조사에 의한 효과에 있어서는 LLB2N2배지의 8 Gy에서 캘러스의 생장에 효과가 가장 좋았고, LLK2I0.2배지에서는 16 Gy에서 캘러스 생장효과가 좋았지만 암배양에서의 ${\gamma}$선처리 효과는 없었다. 명배양에서 LS배지에 BA 1 mg/L와 NAA 1 mg/L를 첨가할 경우, BA 2mg/L와 NAA 2 mg/L를 첨가할 때보다 캘러스의 생장효과가 있었으나, 암배양에서는 차이가 없었다. 특정선량에 대한 BA와 NAA의 농도에 있어서는 각각 2 mg/L가 효과적인 것으로 나타났다. MK2I0.2배지는 0.5 Gy와 1 Gy에서 캘러스의 생장에 효과적이었으나, 기본배지의 종류에 있어서는 MS배지보다 LS배지에서 더 효과적이었다. M-9배지에서는 명배양에서도 캘러스의 생장은 저조하였으나, 16 Gy 및 30 Gy에서 캘러스의 생장이 30%이상 증가하였다. 암배양에서는 ${\gamma}$선 조사에 의한 캘러스의 생장효과는 거의 없었다.

• 농업생명과학연구
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• 제43권2호
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• pp.17-21
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• 2009

본 실험은 폐타이어칩이 심비디움 Pine Clash 'Moon Venus'의 생장 및 양분함량에 미치는 영향에 대해서 조사하였다. 잎, 줄기의 지상부 생장은폐타이어칩과 바크를 혼용한 경우에는 관행구인 바크와 차이가 없었다. 그러나 폐타이칩 단용구에서는 지상부의 생장이 현저하게 감소하였다. 총근수, 신근수, 근장 등 지하부의 생장도 지상부의 생장과 유사한 경향을 나타내었다. 부패근수는 폐타이어칩 중립과 대립의 단용구에서 증가하였다. 전당, 전분, 엽록소와 N, P, K 등 무기양분의 함량도 폐타이어칩과 바크를 혼용한 경우에는 관행구인 바크와 차이가 없었으나, 폐타이칩 단용구에서는 감소하였다.

• 한국유기농업학회지
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• 제17권1호
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• pp.55-62
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• 2009

저면도포 무처리구에서 유기상토에 느타리버섯 폐배지로 생산한 분립 4-6%의 첨가비율에서 벼의 유식물체의 생육이 가장 좋았다. 저면도포 처리구에서는 대조구(유기상토 100%)에서 벼의 유식물체의 생육이 가장 좋았는데, 이는 신장된 뿌리가 토양표면에 시용된 분립으로부터 생육에 필요한 양분을 흡수하였기 때문으로 추정된다 따라서 장기간 육묘할 경우에는 유기상토에 분립을 혼합하지 않고 토양표면에 분립 100%를 시용하는 저면도포 방법이 효율적이라고 생각된다. 느타리버섯 폐배지로 생산한 분립은 중금속 함량이 낮아서 안전성이 높고. 양분공급능력이 높은 유기상토재로서 이웅 가능성이 높다고 판단된다. 유기상토의 양분공급능력을 높여 유식물체의 생육을 촉진하기 위해서는 분립과 같은 상토재의 첨가를 통하여 질소함량은 높이고, 탄질율은 낮추는 것이 필요하다고 판단된다.

• 한국미생물·생명공학회지
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• 제17권3호
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• pp.231-235
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• 1989

부착성 동물세포인 recombinant human embryonic kidney cell line 293을 5% FBS가 함유된 DME/F12(3:1) mixture 배지에서 배양할 경우 static culture에서와는 달리 미립담체 배양에서는 낮은 부착효율과 spreading의 저해현상이 관찰되었다. Cell line 293의 미립담체 배양에 있어서 배지내의 serum은 세포의 미립담체에 대한 부착속도(attach-ment rate)를 감소시킬 분만 아니라 세포의 증식을 저해한다는 사실을 확인하였다. 이러한 경우, conditioned microcarrier를 이용함으로써 미립담체로부터 세포의 탈착을 어느 정도 막을 수 있었다. 특히 autocrine growth factor를 생성하는 hepatoma cell line인 HpG 2 cell을 배양하여 얻은 conditioned medium을 serum-free medium에 첨가하여 사용함으로써 세포의 정상적인 성장 분만 아니라 growth-stimulation을 유도할 수 있었다.

• 한국축산식품학회지
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• 제28권1호
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• pp.105-108
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• 2008

The cell growth and antioxidant activity of Bacillus polyfermenticus SCD were studied in tryptic soy broth (TSB) medium and whey protein concentrate (WPC)-based medium. Overall, higher lactose contents in WPC-35 medium (up to 2.0%), and longer culture times correlated with greater cell viability. In WPC-35 medium with 1.5% and 2.0% lactose, the cell growth of B. polyfermenticus SCD was similar to growth in TSB medium. The 1,1-diphenyl-2-picyrylhydrazyl (DPPH) radical scavenging activity of culture supernatant of B. polyfermenticus SCD in WPC-35 medium was measured to assess antioxidant activity. The antioxidant activity increased up to 32 hr of culture, reaching a maximum of 75.57% DPPH radical scavenging activity. The antioxidant activity seemed to follow the typical kinetics of primary metabolite synthesis. The antioxidant activity of B. polyfermenticus SCD supernatant in WPC-35 medium was more effective and stable than supernatant from TSB medium. These results suggest that WPC-35 medium is effective for the production of antioxidant by B. polyfermenticus SCD.

• Journal of Microbiology and Biotechnology
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• 제23권12호
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• pp.1747-1756
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• 2013

The aim of this paper was to study the effect of spent medium recycle on Spodoptera exigua Se301 cell line proliferation, metabolism, and baculovirus production when grown in batch suspension cultures in Ex-Cell 420 serum-free medium. The results showed that the recycle of 20% of spent medium from a culture in mid-exponential growth phase improved growth relative to a control culture grown in fresh medium. Although both glucose and glutamine were still present at the end of the growth phase, glutamate was always completely exhausted. The pattern of the specific glucose and lactate consumption and production rates, as well as the specific glutamine and glutamate consumption rates, suggests a metabolic shift at spent medium recycle values of over 60%, with a decrease in the efficiency of glucose utilization and an increase in glutamate consumption to fuel energy metabolism. Baculovirus infection provoked a change in the metabolic pattern of Se301 cells, although a beneficial effect of spent medium recycle was also observed. Both growth rate and maximum viable cell density decreased relative to uninfected cultures. The efficiency of glucose utilization was dramatically reduced in those cultures containing the lowest percentages of spent medium, whereas glutamine and glutamate consumption was modulated, thereby suggesting that infected cells were devoted to virus replication, retaining their ability to incorporate the nutrients required to support viral replication. Recycle of 20% of spent medium increased baculovirus production by around 90%, thus showing the link between cell growth and baculovirus production.

• Journal of Plant Biology
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• 제12권2호
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• pp.1-6
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• 1969

Effects of the special media on the mycelium growth in Agaricus campestris were studied. The results might be summarized as follows: 1. The mycelium growth fo Agaricus campestris were scarecely stimulated on the Peptone basal medium which was added 0.5gr. of Peptone and Dextrose basal medium which was added 1.5gr. of dextrose during the culture for 144 hours. 2. The mycelium of Agaricus campestris on the media which was added the several kinds of vegetable extracts showed a considerable growth for 144 hours. The order is as follows; Carrot-basal medium(4ml./100ml.)>Tomato-basal medium(2ml./100ml.)>Spinach-basal medium(3ml./100ml.). However, the spinach-basal medium among these three media were no significant difference.

• Asian Journal of Business Environment
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• 제9권2호
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• pp.27-34
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• 2019

Purpose - From the advanced path of development and current situation, the development of enterprises plays a tremendous role in promoting national economic growth and raising the overall national strength. Therefore, this paper aims at examining the mutual effect between small & medium enterprises and economic growth. Research design, data, and methodology - In order to address the operating mutual effect between the small & medium enterprises and economic growth more clearly, this paper sets Alibaba Group and Hangzhou as an example. Meanwhile, the annual data from 2000 to 2017 will be employed, and an empirical analysis will be performed under the vector error correction model. Results - The findings display that the total revenue of Alibaba Group has a positive effect on economic growth in city of Hangzhou. However, the Granger Causality test implies that there is only a unidirectional causality between total revenue of Alibaba Group and economic growth in Hangzhou. More specifically, 1% increase in total revenue of Alibaba Group can result in 0.272% in economic growth of Hangzhou in the long run. Conclusions - In summary, for the long run, the local governments should promulgate a series of policies to assist the small & medium enterprises like Alibaba Group to improve the local economic growth as seen in the city of Hangzhou.

• 한국약용작물학회지
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• 제10권4호
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• pp.243-248
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• 2002

The effects of basal media, carbon, nitrogen, phosphate and some major macro elements on growth and shikonin production in Lithospermum erythrorhizon hairy root culture were studied. Among examined media, growth of hairy root cultured in B5 liquid medium was rapid, whereas shikonin production was high in MS liquid medium. Under B5 basal medium, sucrose concentration for optimal growth and shikonin production was 9% and 4% respectively. The growth and shikonin production on pH changes in B5 medium resulted little effect in pH 5.8 to pH 8.8 ranges, whereas growth was decreased dramatically in both above 8.8 and under 5.8. Nitrogen source and concentration effected on the growth and shikonin production. The highest growth rate was in B5 medium (50 mM $KNO_3$ and 1 mM $NaH_2PO_4)$, whereas the highest shikonin production was in the condition supplemented with 5 mM $KNO_3$ and 10 mM $NaH_2PO_4$.

• 한국식물병리학회지
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• 제14권4호
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• pp.299-302
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• 1998

V8 juice agar (V8A) has been the most popular and commonly used medium for growth and reproduction of Phytophthora. However, frequently V8 vegetable juice is not available or difficult to obtain in Korea. We therefore developed widely accessible medium to substitute for V8A using domestically available five juices; two carrot (KCJA), two tomato (KTJA) and a vegetable-mix (KVMA). To prepare 10% juice medium, each vegetable juice 100 ml, DW 900 ml, agar 17 g and Ca CO3 0.5∼1.0g were supplemented to adjust pH ca. 6.0 Mycelial growth of P. cactorum and P. capsici on KTJA and KVMA was equally effective as V8A for the growth of P. cactorum, P. capsici, P. drechsleri and P. nicotianae under light. Sporangial production of P cactorum, P. capsici and P. nicotianae on KTJA and KVMA was as good as V8A and slightly better than CKJA, but the difference was insignificant by P. cactorum and P. nicotianae. The four fungi successfully formed oospores on all the media although the numbers were varied among species and media. While KTJA was the best for P. cactorum and P. capsici, V8A was the best for P. capsici and P. drechsleri. However, KCJA stimulated highest number of oopspores of P. nicotianae. Overall results showed that domestically available vegetable juices were highly effective on growth and reproduction of Phytophthora and comparable to V8 juice. Therefore, the domestic juice medium can be successfully replaced V8A in Phytophthora study.