• Journal of Microbiology and Biotechnology
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• v.31 no.10
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• pp.1455-1464
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• 2021

Trehalose is a non-reducing disaccharide in increasing demand for applications in food, nutraceutical, and pharmaceutical industries. Single-step trehalose production by trehalose synthase (TreS) using maltose as a starting material is a promising alternative process for industrial application due to its simplicity and cost advantage. Pseudomonas monteilii TBRC 1196 was identified using the developed screening method as a potent strain for TreS production. The TreS gene from P. monteilii TBRC 1196 was first cloned and expressed in Escherichia coli. Purified recombinant trehalose synthase (PmTreS) had a molecular weight of 76 kDa and showed optimal pH and temperature at 9.0 and 40℃, respectively. The enzyme exhibited >90% residual activity under mesophilic condition under a broad pH range of 7-10 for 6 h. Maximum trehalose yield by PmTreS was 68.1% with low yield of glucose (4%) as a byproduct under optimal conditions, equivalent to productivity of 4.5 g/l/h using enzyme loading of 2 mg/g substrate and high concentration maltose solution (100 g/l) in a lab-scale bioreactor. The enzyme represents a potent biocatalyst for energy-saving trehalose production with potential for inhibiting microbial contamination by alkaline condition.

• Journal of Integrative Natural Science
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• v.17 no.2
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• pp.59-65
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• 2024

Dendritic cells play a very important role in the immune response as antigen-presenting cells that are critical for initiating both innate and acquired immunity. They recognize, process and present foreign antigens to other key immune cells to trigger and regulate the immune response. The ability to activate these dendritic cells can be used as a treatment for various immune diseases. Maqui berry has been reported to have anticancer, antibacterial and anti-inflammatory properties. However, its effect on the activity of dendritic cells has not been studied. In this study, we investigated the efficacy of maqui berry extract in modulating dendritic cell activity. Treatment of dendritic cells with maqui berry extract induced the costimulatory molecules CD80, CD86, and MHC class I and II in a concentration-dependent manner. Furthermore, the antigen-presenting capacity of dendritic cells was inhibited, which confirms their ability to present antigens, and the production of Interleukin (IL)-12, which is important for dendritic cell activity, was increased. These results indicated that Maqui berry extract activates dendritic cells maturation by inducing the production of co-stimulatory molecules and IL-12. These results suggest that maqui berry extract may act as an effective adjuvant to enhance dendritic cell-based immune responses.

• Proceedings of the KWS Conference
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• 2002.10a
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• pp.282-288
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• 2002

A new on-line bar joining technology employing the concept of a solid-state joining has been developed for the endless rolling by POSCO, RIST and MHMM Inc.. In the process, the bars are partially descaled, partially overlapped, joined by shearing action and crops are finally removed. The feasibility of the developed process was evaluated in this study in terms of microstructures and mechanical properties of joints, and the response of the joint to rolling.

• International Journal of CAD/CAM
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• v.3 no.1_2
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• pp.77-83
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• 2003

We propose two algorithms to generate (1) polygonal meshes and (2) developable surface patches far generalized cylinders defined by contours of discrete curves. To solve the contour blending problem of generalized cylinder, the presented algorithms have adopted the algorithm and related properties of LIDM (linear interpolation by direction map) that interpolate geometric shapes based on direction map merging and group scaling operations. Proposed methods are fast to compute and easy to implement.

• The Journal of the Korea Contents Association
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• v.16 no.1
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• pp.766-777
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• 2016

This study is aimed at suggesting the implications about the utility of team system and importance of leadership through the analysis of what influence of transformational leadership and self leadership has on team performance. This study used the LMX theory & social exchange theory arguing that exchange relation quality forms between organizational members, and situational theory stating that leadership suited for business environment should be displayed as the theoretical background of this research. This study, targeting 9 domestic energy-related public enterprises, proved the research hypotheses through regression analysis under SPSS 20.0 by using the effective data on 880 respondents from 166 teams in total. As a result of the research, a team leader's transformational leadership was found to have a positive influence on team performance, and the behaviour strategy, and cognitive strategy of self leadership were found to significantly modulate the relation between a team leader's transformational leadership and team performance, thus getting to adopt all of the initially-set research hypotheses. The salient point was that the effect was found to be much stronger from the group whose self leadership was low. What this research results imply is that if a team leader in team organization should look to it that team members themselves could be self-motivated by assigning goals, which can be put into practice with concern, to the members of a group whose self leadership is relatively low, team performance could improve more clearly than would otherwise be the case.

• Journal of Nutrition and Health
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• v.46 no.3
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• pp.285-295
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• 2013

The purpose of this study was to examine the characteristics of the dietary intake of Korean elderly according to chew-ing ability using data from the Korea National Health and Nutrition Examination Survey (KNHANES) conducted during 2007-2010. Among subjects aged 65 years and over, more than half, 54.3% of elderly people, were classified as the difficulty in chewing group (DC). The DC group had lower nutrients and food intakes than those of in the no difficulty in chewing group (NDC). Findings showed that subjects in the DC group consumed fewer foods, especially fruits and vegetables. In addition, the DC group had significantly lower intakes of pan-fried food, stir-fried food, braised food, and seasoned-cooked vegetables, which could not be easily cooked or chewed. On the other hand, the number of soups and stews included in the top 30 largely consumed dishes were higher in the DC group than in the NDC group. No difference in numbers of daily meal/snack intake was observed between the two groups, however, the DC group had lower numbers of side-dishes compared to the NDC group. Fewer side-dishes per meal could be related to lower intakes of nutrients in dietary quality. Findings of this study demonstrated that dietary intake was influenced by chewing ability of elderly Korean people. Therefore, study of factors affecting dietary intake such as convenient cooking methods to decrease cooking time and skills to extend preservation and storage time of foods will necessary. In addition, development of food products and new techniques of cooking considering health status, chewing, and swallowing ability is required for the elderly, followed by establishment of standards for senior-friendly food products.

• The Korean Journal of Mycology
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• v.48 no.4
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• pp.423-443
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• 2020

Freshwater fungi are a poly-phylogenetic group of taxonomically diverse organisms. In this study, we isolated diverse fungal strains from various environmental samples obtained from freshwaters in Korea. These strains were identified by performing molecular phylogenetic analyses of rDNA and/or other sequences (beta-tubulin, RNA polymerase II, and translation elongation factor 1). In addition, we examined their morphological characteristics microscopically and cultural characteristics using different media. We identified eleven unrecorded Pezizomycotina species: Cladosporium angulosum, Pseudorobillarda phragmitis, Paraconiothyrium estuarinum, Pseudopithomyces palmicola, Pyrenochaetopsis paucisetosa, Thelebolus globosus, Plagiostoma mejianum, Trichoderma cremeum, Fusarium tanahbumbuense, Coniochaeta endophytica, and Chaetomium tenue. Environmental samples obtained from different freshwater ecosystems in Korea could thus be a good source for isolating and investigating novel fungal species.

• Journal of the Optical Society of Korea
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• v.19 no.6
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• pp.629-637
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• 2015

Compact system cameras (CSCs) are commonly used nowadays and feature enhanced video functions and thin yet light interchangeable lenses. They differ from digital single-lens reflex (DSLR) cameras in their lack of mirror boxes. CSCs, however, have autofocus (AF) speeds lower than those of conventional DSLRs, requiring weight reduction of their AF groups. To ensure the marketability of large telephoto zoom lenses with fixed f/2.8 regardless of field angle variation, in particular, light weight AF groups are essential. In this paper, we introduce a paraxial optical design method and present a new, large, telephoto zoom lens with f/2.8 regardless of the field angle variation, plus a lightweight AF group consisting of only one lens. Using the basic paraxial optical design and optimization methods, we fabricated a new and lighter zoom lens system, including a single-lens, lightweight AF group with almost the same performance.

• Korean Journal of Medicinal Crop Science
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• v.20 no.6
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• pp.434-439
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• 2012

This study was conducted to determine the contents of heavy metal in commercial herbal medicines. The monitoring of lead, arsenic, cadmium and mercury was carried out on 100 samples with 10 kinds of herbal medicines. The contents of heavy metal were measured by inductively coupled plasma atomic emission spectrometry (ICP-AES) and mercury analyzer. The mean values of heavy metal contents for herbal medicines were Pb 1.87 mg/kg, As 0.39 mg/kg, Cd 0.26 mg/kg and Hg 0.021 mg/kg. Of the total samples, 19 samples (19%, 4 types) were violated the KFDA regulatory guidance of heavy metal in herbal medicines (9 samples on Pb, 10 samples on Cd). Among these 19 unsuitable samples, 16 samples were domestic and 3 samples were imported. Therefore, we need continuously monitoring to ensure confidence and safety for these herbal medicines.

• Journal of Power Electronics
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• v.11 no.4
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• pp.464-470
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• 2011

In this paper, the feasibility of applying a position sensorless control technique to hybrid electric vehicles (HEVs) is practically evaluated. The proposed position estimator has a straightforward structure with properties that combines the model and the saliency tracking-based rotor position estimation for interior permanent magnet synchronous motors (IPMSMs). The proposed method can be used in the event of sensor loss or sensor recovery to sustain continuity of operations. The developed system takes into account the estimated position transition between two distinct sensorless methods. The transition is enhanced by introducing a synchronized transition algorithm based on a single tracking observer. Extensive experimental results are presented to verify the principles and show a reliable estimation performance over the entire speed range including standstill under 150% load conditions.