• 한국수산과학회지
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• 제35권6호
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• pp.682-685
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• 2002

노래미와 쥐노래미의 유전적인 종 동정의 확립과 우량 품종 개발을 위한 유전 육종학적 연구의 기초 자료를 얻고자, 최근 양식 대상어로 대두되고 있는 두 어종을 대상으로 적혈구 세포와 핵의 크기, DNA 함량, 핵형 분석 등의 세포유전학적 연구를 수행하였다. 노래미의 적혈구 세포의 크기는 장, 단축이 각각 $9.76{\pm}0.27{\mu}m^2$, $6.35{\pm}0.07{\mu}m$로, 쥐노래미의 $9.17{\pm}0.05{\mu}m$, $6.2424{\pm}0.04{\mu}m$ 보다 크게 나타났으며 , 표면적과 부피 역시 노래미가 $48.62{\pm}1.74{\mu}m^2$, $213.67{\pm}7.51{\mu}m^3$로 쥐노래미의 적혈구 세포 표면적 $44.85{\pm}0.44{\mu}m^2$, 부피 $187.57{\pm}2.45{\mu}m^3$보다 큰 것으로 나타났다. 또한 노래미의 DNA 함량은 2.15$\pm$0.04pg, 쥐노래미는 2.10$\pm$0.03pg으로 핵의 크기와 유사한 양상을 나타내었다. 노래미와 쥐노래미의 염색체수는 48개로 동일한 핵형으로 구성되어 있었으며, NOR분석 결과 역시 두 종에서 1쌍의 acrocentric chromosome의 short arm에서 NOR이 확인되었다. 성별에 따른 염색체의 수적 차이나 hetero-morphic한 염색체, 그리고 개체간 염색체 다형 현상은 관찰되지 않았다.

• 환경생물
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• 제24권2호
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• pp.112-118
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• 2006

본 연구는 쥐노래미과(Hexagrammidae)에 속하고 양식산업에서 중요한 위치를 차지하고 있는 노래미, Hexagrammos otakii(Jordan et Starks)와 쥐노래미, H. agrammus(Temminck et Schlegel)의 미토콘드리아 cytochrome b(cyt b)유전자의 염기서열을 서로 비교 분석하였다. 총 489 bp크기의 cyt b 염기서열에서 종간의 변이는 거의 없었다(96%의 유사성).Neighbor-joining방법에 의한 노래미와 쥐노래미에서의 0.0342인 pairwise distance는 두 종이 분자계통유전학적으로 유사함을 시사한다. 이러한 연구 결과들은 쥐노래미(Hexagrammos)속을 대상으로 한 양식, 수산유전학 및 분자계통학 분야에 적용 가능할 것이다.

• Fisheries and Aquatic Sciences
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• 제16권1호
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• pp.35-39
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• 2013

The aim of this study was to assess visible implant fluorescent elastomer (VIE) tagging in greenling Hexagrammos otakii. The experiental fish were anesthetized individually and marked with orange, yellow, red, and green elastomer at the following five body locations, respectively: the adipose eyelid, the surface of the dorsal fin base, the inside surface of the pectoral fin base, the inside surface of the pelvic fin base, and the surface of the anal fin base. Control fish were anesthetized but not marked. During the 20-month trial, fish growth and retention, underwater visibility, and readability of the tags were determined. After 20 months, body length of marked greenling ($43.2{\pm}3.5cm$, mean ${\pm}$ standard deviation [SD]) did not differ from that of the control ($41.4{\pm}3.7cm$). Additionally, the body weight of marked greenling ($527.4{\pm}39.8g$, mean ${\pm}$ SD) did not differ from that of the controls ($505.9{\pm}31.7g$). Greenling retained >90% of the tags at the surface of the dorsal fin base. The anal fin base showed a higher tag retention rate than the inside surfaces of the pectoral fin and the pelvic fin bases (P < 0.05). Red and orange tags were identified more easily underwater than green and yellow tags. Green and yellow tags emitted fluorescence in response to a narrower range of light wavelengths. Thus, the VIE mark was easy to apply to greenling (< 1 min per fish) and was readily visible when viewed under an ultraviolet lamp.

• Fisheries and Aquatic Sciences
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• 제14권4호
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• pp.303-310
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• 2011

We characterized a cytoplasmic actin gene locus in greenling Hexagrammos otakii (Scorpaeniformes). Genomic clones isolated from the greenling DNA library contained two homologous cytoplasmic actin gene copies (HObact2.1 and HObact2.2) in a tail-to-head orientation. Their gene structure is characterized by six translated exons and one non-translated exon. Exon-intron organization and the nucleotide sequences of the two actin gene isoforms are very similar. However, only the HObact2.1 isoform contains microsatellite-like, dinucleotide repeats in the 5'-flanking region (named HOms2002) and intron 1 following the non-translated exon 1 (named HOms769). One microsatellite locus (HOms769) was highly polymorphic while the other (HOms2002) was not. Based on bioinformatic analysis, different transcription factor binding motifs are related to stress and immune responses in the two actin isoforms. Semiquantitative and real-time reverse transcription-PCR assays showed that both isoform transcripts were detectable ubiquitously in all the tissues examined. However, the basal expression levels of each isoform varied across tissues. Overall, the two isoforms showed a similar, but not identical, expression pattern. Our data suggest that the cytoplasmic actin genes may be the result of a recent duplication event in the greenling genome, which has not experienced significant subfunctionalization in their housekeeping roles.

• 수산해양기술연구
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• 제48권2호
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• pp.137-146
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• 2012

Black porgy and fat greenling are commercially important fish species due to the continuously increasing demand in Korea. When estimating acoustically the fish length by a fish sizing echo sounder, it is of crucial importance to know the target strength (TS) to length dependence. In relation to these needs, the target strength experiments for live fishes were conducted in an acrylic salt water tank using two split-beam echo sounders operating at 70 and 120kHz. The target strength under well-controlled laboratory conditions was simultaneously measured with the swimming movement by digital video recording (DVR) system and analyzed as a function of fish length (L) and frequency (or wavelength ${\lambda}$). Equations of the form TS-alog (L)+blog (1)+c were derived for their TS-length dependence. The best fit regression of TS on fork length for black porgy was TS=20.62 log (L, m)-0.62 log (${\lambda}$, m)-30.68 ($r^2$=0.77). The best fit regression of TS on fork length for fat greenling was TS=12.06 log (L, m)-5.85 log (${\lambda}$, m)-22.15 ($r^2$=0.44).

• 수산해양기술연구
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• 제39권3호
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• pp.174-180
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• 2003

서해안의 연안성 어종인 쥐노래미는 백령, 대청 근해에서 주요 어종의 하나로 주로 통발에 의해 어획되고 있다. 쥐노래미 어업이 지속적으로 유지되기 위해서, 소형어를 보호하는 효율적인 어구 사용과 현재 정해져 있는 망목크기가 적정한가를 파악하기 위하여 망목선택성 실험을 하였다. 쥐노래미 통발에 대한 망목선택성을 Kitahara의 방법에서 객관성이 있도록 선택성 곡선을 다항식이나 로지스틱 곡선식을 이용하여 구하고, 쥐노래미의 최소성숙체장에 대한 적정망목크기를 구하였다. 통발의 경우, 선택성 곡선(마스터 곡선)을 구할 때 적용한 다항식은 삼중자망에서와는 달리 어체가 큰 부분에서 보정치가 지나치게 작용하여 선택성 곡선을 신뢰할 수 없었고, 로지스틱 곡선이 보다 합리적이었으며, 그 식은 다음과 같았다. s(R) =(equation omitted) 쥐노래미의 최소성숙체장은 21.4㎝로 알려져 있으며, 이 최소성숙체장에 대해 50% 어획될 확률 (L/sub 50/)이 되는 망목크기는 37.0㎜이었다. 현재 통발의 망목크기 제한은 35㎜로 규정되어 있는데 쥐노래미의 경우에 다소 작으나 큰 차이는 없는 것 같다.

• 한국수산과학회지
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• 제40권2호
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• pp.84-94
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• 2007

The comparative feeding ecology of greenling Hexagrammos otakii and Schlegel's black rockfish Sebastes schlereli populations was investigated in the Jangbong tidal flat near Incheon, Korea. Monthly samples were taken using a modified otter trawl from November 1999 to January 2001. The stomach contents of 124 greenling and 115 Schlegel's black rockfish were analyzed. The diet of H. otakii was dominated by the amphipods Gammaropsis iaponicus, Caprella scaura, Isaeopsis sp. and Monocorophium acherusicum, the shrimps Latreutes mucronatus and Alpheus japonicus, the mysid Neomysis orientalis and the bivalve Mytilus edulis. In contrast, S. schlegeli mainly fed on the mysids N. orientalis, N japonica, and N. awatschensis, the shrimps Exopalaemon carinicauda, A. japonicus and L. mucronatus, and the fish Pholis fangi and unidentified gobiids. A comparison of the frequency and abundance of food items showed that crustaceans (e.g. amphipods, mysids and shrimps) were important foods for both species. Their main dietary components, however differed which is probably due to differences in the spatial distribution of the two species and their prey items. In conclusion, greenling and Schlegel's black rockfish, despite being sympatric on a tidal flat have distinct food habits and low dietary overlap (Schooner's index: 0.1). These results indicate, to some extent, resource partitioning for the maximum utilization of available foods in the tidal flat.

• 수산해양교육연구
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• 제15권2호
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• pp.135-144
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• 2003

1. The appearance of fisheries animals and plants of investigation areas were fishes, 20 species; algae, 9 species; molluska, 6 species; arthropod, 12 species; echinoderm, 5 species by confirmed appearance species. 2. The C.P.U.E by trap(1pcs of trap) were 3.95 species and weight of 2,098g, mainly catching species are greenling and jacopever(94.16%). The C.P.U.E by long liner(long liner 1coil, 100pcs) were 11.7species and weight of 6,418g, It occupied jacopever(35.63%) greenling(20.78%), bastard halibut(20.31%). The C.P.U.E of appearance species gill net(ipcs of gill net) were 5.05species and weight of 3,050g, It occupied jacopever(39.63%) greenling(29.83%), bastard halibut(17.47%). 3. The results of comparative analysis and natural species and stocked species, in case of bastard halibut, jacopever species were occupied species(83.33~92%), weight(86.86~96%). The stocked species were occupied species of 8.00~16.67%, weight of 4.00~13.14%. Provided the stocked projets were enacted effectively in future. The density of dwelling of stocked species were increase. Also, it will be contribute to the increase of fishermens income.

• 한국수산과학회지
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• 제36권5호
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• pp.449-453
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• 2003

Anaesthetic effect of lidocaine hydrochloride-sodium bicarbonate mixture $(lidocaine\;HCL/NaHCO_3)$ and tricaine methanesulfonate (MS-222) was tested for the greenling (Hexagyammos otakii) at three different temperature regimes: $12^{\circ}C,\;8^{\circ}C\;and\;24^{\circ}C.$ Based on the exposure and recovery time, effective dose of lidocaine $HCl/NaHCO_3$ was 800 ppm $(18^{\circ}C)\;and\;300\;ppm\;(24^{\circ}C)$ for greenling of $21.0\pm1.4\;cm$ body length. Anaesthetic dose and temperature-dependent relationship in exposure and recovery time were observed. Effective dose of MS-222 at $18^{\circ}C$ was proven to be 125 ppm and 150 ppm. Combination of lidocaine $HCl/NaHCO_3$ and MS-222, considerably reduced the dosage of each anaesthetic required to give rapid, deep anaesthetic condition. In the dry exposure after anaesthetic, the control fish exhibition $22\%$ mortality after dry exposure of 20 min; whereas, the anaesthetic condition with 800 ppm lidocaine $HCl/NaHCO_3$ for 1 min exhibited delayed recoveries from the anaesthetic condition with mortalities of $20\%,\;41\%,\;78\%\;and\;100\%$ after dry exposures of 8 min, 12 min, 16 min and 20 min, respectively. The results indicate that lidocaine $HCl/NaHCO_3$ can be used as suitable anaesthetic for the greenling.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2006년도 수산관련학회 공동학술대회 발표요지집
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• pp.342-343
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• 2006