• Journal of the Korea Organic Resources Recycling Association
• /
• v.11 no.1
• /
• pp.91-91
• /
• 2003

시험은 대조구 0%와 남은 음식물에 Protein의 요구량 수준을 각각 높인 10%, 20%, 30%, 40%, 대조구에 녹차 부산물(GTB) 첨가구 1.0%, 남은 음식물 30% + 녹차 부산물 (GTB) 1.0%로 총 7처리 4반복으로 하였으며, 반복당 6수씩 개체별로 사양 실험을 실시하고, 산란율, 난중, 난각두께, 난황색, Thiobarbituric acid (TBA) 및 관능검사 등을 측정한 결과는 다음과 같다. 1. 산란율은 남은 음식물 10%, 20% 및 40% 첨가구에서 각각 82.65%, 81.54% 및 82.27%로 높게 나타났으나 남은 음식물 30%첨가구는 75.34%로 낮게 나타났으며 통계적 유의차를 보였다 (P<0.05). 대조구가 72.87%가 가장 낮게 나타났으며 (P<0.05), 주령별로는 주령이 증가할수록 산란율이 다소 감소하는 경향을 보였다. 2. 난중은 전 처리구중 가장 높은 경향을 보인 것은 남은 음식물 30% + GTB 1%처리구가 63.78로 높은 경향을 보였다. 가장 낮은 난중은 남은 음식물 10%처리구가 60.02로 나타났다 (P<0.05). 3. 산란량은 전 처리구에서 남은 음식물 30% + GTB 1%가 52.53g으로 높게 나타났고, 가장 낮은 산란량을 보인 것은 대조구가 45.32g으로 가장 낮게 나타났으며 통계적 유의차를 보였다 (P<0.05). 4. 사료 요구율은 남은 음식물 20%첨가구가 2.86으로 높게 나타났으며, 낮은 요구율은 남은 음식물 10%첨가구가 2.47으로 낮게 나타났으며 통계적 유의차를 보였다 (P<0.05). 전 처리구간에서 사료 요구율은 주령이 증가할수록 요구율이 증가하는 경향을 보였다. 5. 콜레스테롤은 대조구 + GTB 1.0%첨가구가 13.923으로 높게 나타났다. 가장 낮은 콜레스테롤은 남은 음식물 40%처리구가 12.003으로 낮게 나타났다. 남은 음식물을 첨가한 처리구가 난황 내 콜레스테롤 함량이 낮아지는 경향을 보였으나 남은 음식물 30%첨가구가 12.835로 높은 수치를 보였으며 통계적 유의차는 나타나지 않았다 (P>0.05). 6. TBA가 가장 높은 처리구간은 1주에는 남은 음식물 40%첨가구가 3.205로 높게 나타났으며 2주에는 남은 음식물30%첨가구가 3.275로 높게 나타났다. 마지막 주에는 3.947로 높게 나타났다. 이 결과로 볼 때 남은 음식물을 첨가한 처리구가 대체적으로 산패도가 높게 나타나는 경향을 보였으며 통계적 유의차는 나타나지 않았다 (P>0.05).

• Journal of Food Hygiene and Safety
• /
• v.27 no.3
• /
• pp.317-324
• /
• 2012

In order to determine an authenticity of food ingredient, we used DNA barcode method by universal primers. For identification of animal food ingredients, LCO1490/HCO2198 and VF2/FISH R2 designed for amplifying cytochrome c oxidase subunit1 (CO1) region and L14724/H15915 for cytochrome b (cyt b) region on mitochondrial DNA were used. Livestock (cow, pig, goat, sheep, a horse and deer) was amplified by LCO1490/HCO 2198, VF2/FISH R2 and L14724/H15915 primers. Poultry (chicken, duck, turkey and ostrich) was amplified by LCO1490/HCO 2198 and VF2/FISH R2 primers. But, Fishes (walleye pollack, herring, codfish, blue codfish, trout, tuna and rockfish) were only amplified by VF2/FISH R2 primers. For plant food ingredients, 3 types of primers (trnH/psbA, rpoB 1F/4R and rbcL 1F/724R) have been used an intergenic spacer, a RNA polymerase beta subunit and a ribulose bisphosphate carboxylase region on plastid, respectively. Garlic, onion, radish, green tea and spinach were amplified by trnH/psbA, rpoB 1F/4R and rbcL 1F/724R. The PCR product sizes were same by rpoB 1F/4R and rbcL 1F/724R but, the PCR product size using trnH/psbA primer was different with others for plants each. We established PCR condition and universal primer selection for 17 item's raw materials for foods and determine base sequences aim to PCR products in this study. This study can apply to determine an authenticity of foods through making an comparison between databases and base sequences in gene bank. Therefore, DNA barcode method using universal primers can be a useful for species identification techniques not only raw materials but also processed foods that are difficult to analyze by chemical analysis.

• Journal of Aquaculture
• /
• v.19 no.4
• /
• pp.275-280
• /
• 2006

Effect of the various sources of dietary additives on growth, body composition and shell color of abalone Haliotis discus hannai was investigated for 16 weeks. Forty juvenile abalone averaging 13.5 g were randomly stocked into 21 of 50 L plastic rectangular containers each. Eight kinds of additives were prepared for this study: four commercially available microalgae [Haeatococcus (Hae), Isochrysis galbana (Iso), Shizochytrium (Sch) and Spirulina (Spi)], three crustacean meals [krill meal (KM), shrimp head meal (Shm) and red crab meal (Rcm)], and green tea by-product (Gre). In addition, dry sea tangle (Dst), Laminaria japonica, as a control, was prepared. Casein, dextrin and a mixture corn oil and fish oil was protein, carbohydrate and lipid sources, respectively, in the experimental diets. The 2% each additive was included into the experimental diets. The experimental diets were fed to abalone once a day at the ratio of $1.5{\sim}2.0%$ total biomass of abalone with a little leftover throughout the 16-week feeding trial. Survival of abalone was not significantly (P>0.05) affected by the experimental diets. However, weight gain of abalone fed the all experimental diets containing the various sources of additives was significantly (P<0.05) higher than that of abalone fed the Dst diet. Weight gain of abalone fed the Spi diet was highest and Shi, KM and Iso diets in order. Shell length and the ratio of soft body weight to body weight of abalone was not significantly (P>0.05) affected by the experimental diets. However, shell width of abalone fed the all experimental diets containing the various sources of additives was significantly (P<0.05) higher than that of abalone fed the Dst diet. The shell color of abalone fed the Spi diet was improved the most distinctively and similar to that of natural abalone. Therefore, it can be concluded that the experimental diets with the various sources of additives (microalgae and crustacean meals) was effective to improve growth of abalone and dietary inclusion of Spirulina was most effective to improve shell color of abalone.