• Journal of Biosystems Engineering
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• v.31 no.6 s.119
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• pp.514-523
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• 2006

Production of green pepper has been increased due to customer's preference and a projected ten-year boom in the industry in Korea. This study was carried out to develop an automatic grading and sorting system for green pepper using machine vision. The system consisted of a feeding mechanism, segregation section, an image inspection chamber, image processing section, system control section, grading section, and discharging section. Green peppers were separated and transported using a bowl feeder with a vibrator and a belt conveyor, respectively. Images were taken using color CCD cameras and a color frame grabber. An on-line grading algorithm was developed using Visual C/C++. The green peppers could be graded into four classes by activating air nozzles located at the discharging section. Length and curvature of each green pepper were measured while removing a stem of it. The first derivative of thickness profile was used to remove a stem area of segmented image of the pepper. While pepper is moving at 0.45 m/s, the accuracy of grading sorting for large, medium and small pepper are 86.0%, 81.3% and 90.6% respectively. Sorting performance was 121 kg/hour, and about five times better than manual sorting. The developed system was also economically feasible to grade and sort green peppers showing the cost about 40% lower than that of manual operations.

• Journal of Biosystems Engineering
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• v.37 no.2
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• pp.90-99
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• 2012

Purpose: This study was conducted to investigate the optimal sorting factors in establishing an efficient sorting technology for brown rice. Methods: The brown rice varieties used in this study were Il Pum, Chu Cheong, Dong Jin, Un Gwang, Nam Pyeong, and Dae An. These were classified into whole grain, unriped grain, and green dead rice. The shape factors were analyzed based on length, width and thickness of the grains. Results: The results revealed that the maximum length among whole grain, unriped grain, and green dead rice was observed in Dae An variety while Chu Cheong variety showed the minimum. Further more, Il Pum brown rice showed the maximum width while Dong Jin variety showed the minimum. In the case of thickness, the maximum was observed in Un Gwang variety and that of the minimum among Nam Pyeong variety for both whole grain and unriped grain. Conclusions: The length and width can be used as determinants in sorting factors of whole grain and green dead rice, and the thickness can be considered as optimum sorting factor of whole grain and unriped grain.

• Journal of the Korean Wood Science and Technology
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• v.20 no.2
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• pp.51-60
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• 1992

This study was performed to investigate the effect of green sorting before drying on the high-temperature drying characteristics of southern yellow pine dimension lumber(2"${\times}$6"${\times}$12'). To test the advantages of weight sorting, green lumber was seperated into heavy(above 55 1b), medium(50-55 1b), and light(below 50 1b)weight classes. Pieces in each weight class were subgrouped into high(above 35%) and low(below 30%) latewood groups. Groups were dried and seperated by a standard commercial high-temperature schedule; dry bulb temperature $245^{\circ}$ F, wet bulb temperature $180^{\circ}$ F, and air velocity 1200fpm. The results obtained were as follows; 1. There was a highly significant correlation between annual rings per inch(X) and percent-latewood(Y). The regression equation was Y=24, 5047+1.3272X. 2. There were highly significant correlations between either annual rings per inch($X_1$) or percent-latewood($X_2$) a.d specific gravity in green wood(Y). Their regression equations were Y=0.4260+0.0081$X_1$ and Y=0.3749+0.0029$X_2$, respectively. 3. Heavier weight charges dried more slowly than lighter weight charges. 4. Board-to-board variation in green or dry moisture content was less for all seperate weight classes than for unseperated control charges. 5. Lower latewood pieces had higher initial moisture content than higher latewood pieces, and then drying time for lower late wood pieces was longer than higher latewood pieces.

• Journal of Biosystems Engineering
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• v.26 no.6
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• pp.571-578
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• 2001

Production of green pepper has increased for ten years in Korea, as customer's preference of a pepper tuned to fiesta one. This study was conducted to develop an on-line fading algorithm of green pepper using machine vision and aimed to develop the automatic on-line grading and sorting system. The machine vision system was composed of a professive scan R7B CCD camera, a frame grabber and sets of 3-wave fluorescent lamps. The length and curvature, which were main quality factors of a green pepper were measured while removing the stem region. The first derivative of the thickness profile was used to remove the stem area of the segmented image of the pepper. A new boundary was generated after the stem was removed and a baseline of a pepper which was used for the curvature determination was also generated. The developed algorithm showed that the accuracy of the size measurement was 86.6% and the accuracy of the bent was 91.9%. Processing time spent far grading was around 0.17 sec per pepper.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2001.07a
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• pp.308-313
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• 2001

• Journal of Environmental Science International
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• v.23 no.7
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• pp.1349-1358
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• 2014

This study suggests plan of green space management based on the result of research apprehending the characteristic through sorting types of city thermal environment targeting summer which thermal pollution is the most serious. Considering anthropogenic heat, development level of wind road, thermal environment, as a result of types of thermal environment process, it is appeared 36 types, and 10 types is relevant of this research subject. Type I-1, size of building is large, artificial covering area is wide, and thermal load of anthropogenic heat is high, type II-1, development condition of wind road is incomplete as IIlevel, entering cold air is difficult and thermal management and improvement is needed area. Type III-1, scale is large and it is area of origin of cold air, development level of wind road is mostly favorable, type III-2 is revealed as smaller scale than III-1, and small area of origin of cold air. Type IV, anthropogenic heat is $81{\sim}150W/m^2$, average, but development function of wind road is very favorable. Type V, large area of thermal load and the origin of cold air are distributed as similar ratio, and level of development function of wind road is revealed as II level. According to standard of type classification of thermal environment, as a result of suggesting plan of green space management and biotops area ratio, type I-1 is buffer green space and waterway creation, goal biotops area ratio 35%, type II-1 afforestation in site and goal biotops area ratio 40%, type III-1, preservation plan to display the current function continuously is requested. Type IV suggests afforestation of stream current, and type V suggests quantitative increase of green space and goal biotops area ratio 45%.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.5
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• pp.743-748
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• 2017

Objective: Based on rapid advancement of genetic modification techniques, genomic editing is expected to become the most efficient tool for improvement of economic traits in livestock as well as poultry. In this study, we examined and verified the nickase of mutated CRISPR-associated protein 9 (Cas9) to modulate the specific target gene in chicken DF1 cells. Methods: Chicken myostatin which inhibits muscle cell growth and differentiation during myogenesis was targeted to be deleted and mutated by the Cas9-D10A nickase. After co-transfection of the nickase expression vector with green fluorescent gene (GFP) gene and targeted multiplex guide RNAs (gRNAs), the GFP-positive cells were sorted out by fluorescence-activated cell sorting procedure. Results: Through the genotyping analysis of the knockout cells, the mutant induction efficiency was 100% in the targeted site. Number of the deleted nucleotides ranged from 2 to 39 nucleotide deletion. There was no phenotypic difference between regular cells and knockout cells. However, myostatin protein was not apparently detected in the knockout cells by Western blotting. Additionally, six off-target sites were predicted and analyzed but any non-specific mutation in the off-target sites was not observed. Conclusion: The knockout technical platform with the nickase and multiplex gRNAs can be efficiently and stablely applied to functional genomics study in poultry and finally adapted to generate the knockout poultry for agribio industry.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2003.02a
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• pp.335-340
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• 2003

들깻잎은 식생활의 변화로 인한 육류소비의 증가와 더불어 수요량이 급격히 증가하고 있다. 들깻잎은 특유의 향취로 인해 육류나 생선회, 영양탕 등의 음식점에서 상추와 함께 대량소비가 되고 있는 실정이다. 들깻잎의 수확은 반촉성 재배시 40-50일 뒤부터 수확하게 되며 봄가을에는 10일, 여름에는 5-7일 간격으로 수확한다. 들깻잎의 수확기간은 만생종은 150일, 조생종은 110일 정도이다. 1회 수확 시 보통 주당 1-2장(1마디)의 잎을 수확하며 숙련된 노동자의 경우 7,000-1l,000장 정도의 잎을 수확할 수 있다. (중략)

• Journal of Microbiology and Biotechnology
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• v.30 no.9
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• pp.1430-1435
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• 2020

Bacterial cellulose (BC) has outstanding physical and chemical properties, including high crystallinity, moisture retention, and tensile strength. Currently, the major producer of BC is Komagataeibacter xylinus. However, due to limited tools of expression, this host is difficult to engineer metabolically to improve BC productivity. In this study, a regulated expression system for K. xylinus with synthetic ribosome binding site (RBS) was developed and used to engineer a BC biosynthesis pathway. A synthetic RBS library was constructed using green fluorescent protein (GFP) as a reporter, and three synthetic RBSs (R4, R15, and R6) with different strengths were successfully isolated by fluorescence-activated cell sorting (FACS). Using synthetic RBS, we optimized the expression of three homologous genes responsible for BC production, pgm, galU, and ndp, and thereby greatly increased it under both static and shaking culture conditions. The final titer of BC under static and shaking conditions was 5.28 and 3.67 g/l, respectively. Our findings demonstrate that reinforced metabolic flux towards BC through quantitative gene expression represents a practical strategy for the improvement of BC productivity.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.326-333
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• 1993

Okinawa Prefecture is located in the southernmost part of Japan and has a subtropical climate. A lot of tropical fruits such as pineapples, mangoes and papayas are produced. Pineapples were mainly supplied to the canning industry. Since April 1990, the Japanese Government released the foreign trade restriction of canned pineapples and pineapple juice in accordance with GATT. Okinawan Farmers have been expanding the customers from the processing industry to the fresh market. During the recent year, fully mature, high quality pineapples which have 15 Brix in sugar content are produced in green houses on Okinawa. Prices are 2 to 3 times those of the imported pineapples. At present, they are sorted manually, and this sorting might cause the pineapples to lose their reputation. NIR applied fruit sorters have been developed and operated for apples and peaches in Japan . NIR applied tests were carried out to consider the feasibility into the NIR technique for measurement of the NIR a alysis suggests adequate correlations between sugar content and NIR reflectance properties of pineapples and mangoes.