• 한국디지털건축인테리어학회논문집
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• 제13권2호
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• pp.5-16
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• 2013

With regard to the new town developments which have supplied lots of houses in a short period of time in Korea this study aims to evaluation and finding out problems of the developments finally to suggest the direction. A new town's competitiveness model set in the previous paper takes a role of research frame to recognize the problems and to show the direction. The model explains that new town's competitiveness is composed of 4 factors; Self-sufficiency, Innovativenss, Identity and Sustainability. Problems of the developments are as follows; incongruity of spatial structure especially in the capital region, deficiency of self-sufficiency resulted from single-use development, restriction on mixed development by a number of regulations in capital region, low business value, grand scale of land compensation, house oriented planning guidance, unfair share of infrastructure fee, and physical structure depending mainly on fossil energy. Based on this recognition this study conclusively suggests corresponding direction such as role performance as a means of urban growth management, promotion of quality of life by accumulating social capital, introduction of socially sustainable management program for the new towns, discovery and creation of town's value, reexamination of self-sufficiency's meaning or target, selective deregulation of metropolitan development, institutional strategy for cost reduction, changeover from house index to urban function oriented index, and pursuit of low-carbon green town.

• BMB Reports
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• 제32권1호
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• pp.92-97
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• 1999

Cytokinesis and septation should be coordinated to nuclear division in the cell division cycle for precise transmission of the genome into daughter cells. byr4, an essential gene in fission yeast Schizosaccharomyces pombe, regulates the timing of cytokinesis and septation in a dosage-dependent manner. We examined the intracellular localization of the Byr4 protein by expressing byr4 as a fusion of green fluorescence protein (GFP). The Byr4 protein localizes as a single dot on the nuclear periphery of interphase cells, duplicates before mitosis, and the duplicated dots segregate with the nuclei in anaphase. The behavior of Byr4p throughout the cell cycle strongly suggests that Byr4p is localized to the spindle pole body (SPB), a microtubule organizing center (MTOC) in yeast. The presence of the Byr4 protein in the SPB is consistent with its function to coordinate mitosis and cytokinesis. We also mapped the domains of Byr4p for its proper localization to SPB by expressing various byr4 deletion mutants as GFP fusions. Analyses of the diverse byr4 deletion mutants suggest that the indirect repeats and the regions homologous to the open reading frame (ORF) YJR053W of S. cerevisiae in its C-terminus are essential for its localization to the SPB.

• ALGAE
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• 제18권3호
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• pp.191-197
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• 2003

Histones are important proteins that interact with the DNA double helix to form nucleosome. Two putative histone genes, GjH2A-1 and GjH2A-2 were isolated from a red alga Griffithsia japonica. The putative open reading frame of GjH2A-1 and GjH2A-2 shared high similarity with the previously reported amino acid sequences of histone H2As. They have a motif consisting of seven amino acids A-G-L-Q-F-P-V, which matches the histone H2A motif [AC]-G-L-x-F-P-V. Phylogenetic trees were constructed from amino acid sequences of 38 histone H2As. The histone H2As were divided into two groups: major H2As and H2A.F/Z variants. The major histone H2A group consisted of animals, fungi, plants + green algae, and red algae H2A subgroups. The animal histone H2A subgroup was divided into vertebrates, echinoderms, nematodes, insects, and segmented worms H2As. The putative red algal histone genes, GjH2A-1 and GjH2A-2, constituted an independent lineage. This is the first report on red algal histone genes.

• ALGAE
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• 제18권3호
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• pp.183-190
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• 2003

Except for a group I intron in trnL-uaa occuring in eubacteria and plastids, group I introns are rarely documented in plastid genomes. Here, we report that a green alga, Caulerpa sertularioides, contains three group IA3 introns in the 16S gene (cpSSU), CS-cpSSU.i1, CS-cpSSU.i2 and CS-cpSSU.i3. Each intron has an open reading frame with LAGLIDADG motifs. CS-cpSSU.i1orf and CS-cpSSU.i3orf occur at Loop 6 in the intron secondary structure and CScpSSU. i2orf at Loop 8. CS-cpSSU.i1orf and CS-cpSSU.i2orf contain both LAGLI-DADG motifs but CS-cpSSU.i3orf has only one. CS-cpSSU.i1 and CS-cpSSU.i2 share the insetion sites and the ORFs at Loop 6 and 8 with CpSSU·1 and CpSSU·2 introns of Chlamydomonas pallidostigmatica (Chlorophyceae). In contrast, CS-cpSSU.i3, containing 28 copies of GAAATAT at Loop 6, is a novel intron found only in Caulerpa sertularioides. Possible scenarios of the evolution of the three introns and their possible use in systematic research are discussed.

• ALGAE
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• 제27권1호
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• pp.55-62
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• 2012

A novel lectin specific to N-acetyl-D-galactosamine as well as N-acetyl-D-glucosamine was isolated from Bryopsis plumosa and named as BPL-4. Sodium dodecyl sulfate polyacrylamide gel electrophorese (SDS-PAGE) and matrix-assisted laser desorption / ionization-time of flight (MALDI-TOF) mass spectrometry data showed that this lectin was a monomeric protein with molecular weight 12.9 kDa. The N-terminal amino acid sequences of the lectin were determined by Edman degradation and the full cDNA sequence encoding this lectin was obtained using the degenerate primers designed from the amino acid sequence. The size of the cDNA was 414 bp containing single open reading frame (ORF) encoding the lectin precursor. The homology analysis showed that this lectin might belong to H lectin group. BPL-4 showed high sequence similarity (60.6%) to BPL-3, which is a previously reported lectin from the same species. The comparative analysis on the lectin's primary structure showed two conserved domains including one possible active domain of H lectin group.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2009년도 춘계학술대회 논문집
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• pp.187-190
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• 2009

Recently, automotive companies have invested in vehicle weight reduction and clean car development because of oil price rises and environmental problems. In particular, USA car makers have developed the vehicle spending 1 liter per 34km complying with PNGV(Partnership for a new generation of vehicle) and Europe car makers have developed the vehicle spending 3 liters per 100km. The USA government announced "The green car policy" in order to boost production of more fuel effective cars in 2009. According to the policy, it will be restricted to sell the car which spends more than 1 liter per 14.9km by 2020. To satisfy the current situations on automotive market, hydroforming technology has widely adapted vehicle structures such as engine cradle, chassis frame, A pillar, radiator support, etc. However, automotive companies have to consider formability and performance to improve and maximize the benefit from this technology in advance of detail design. The paper deals with one of the vehicle weight reduction methods using tube hydroforming technology and platform commonality in front suspension. FEA simulation is also introduced to evaluate hydro-formability and NVH performance at the beginning of design stage which is the best way to reduce the failure cost.

• KIEAE Journal
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• 제10권1호
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• pp.25-31
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• 2010

An experimental investigation of composite beams composed of wide flange steel and precast concrete is presented. The bottom flange of the steel section is encased in precast concrete. The composite beams tested in this study were designed to reduce the depth of the slab and beam. The slabs are constructed on top of the edges of the Structural Composite Hybrid System, instead of on top of the steel flange, decreasing the depth of the beams. When concrete is cast on the metal deck plate located on the edges of the precast concrete, the weight of the concrete slabs and other construction loads must be supported by the contacts between the steel and the precast concrete. This interface must not exhibit bearing failures, shear failures, and failures caused by torque due to the loading of the precast concrete. When the contact area between the concrete and the bottom flange of the steel beam is small, these failures of the concrete are likely and must be prevented. The premature failure of precast concrete must not also be present when the weight of the concrete slabs and other construction loads is loaded. This paper presents a load carrying capacity of Structural Composite Hybrid System in order to observe the failure mode. The symmetrically distributed loading that caused the failure of the composite beam was found. The paper also provides design recommendations of such type of composite structure.

• 교육시설 논문지
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• 제26권6호
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• pp.29-37
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• 2019

The research institute has a function to get the research outcome through the various experiments, data collection and analysis. Therefore, research building is important to keep the research condition or experiment environment. But buildings would be deteriorated and leaded into the deterrence of research. Maintenance is planned to protect the research building condition through various general repair or heavy repair. The heavy repair is generally conducted in massive repair scope or main components preparation. In this paper, it aimed at analyzing the effect of the massive or main components repair with inputted cost and its resulted output. In order to analyze the effect of a massive repair, it used the Benefit/Cost analysis and sensitivity analysis. Results of this study are as follows : The benefit/cost analysis shows that research building whose researcher continuously live and study has good effect. On the contrary, pace of the the experimental function is not good effect in benefit/cost analysis. But the experimental function is indispensible to get the research outcom for the research goal. Therefore, the experimental function will be planned to repair and get the historical repair data because the proper repair time would be prepared to cut down the repair cost.

• 대한교통학회지
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• 제26권4호
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• pp.161-172
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• 2008

국토해양부는 신호교차로 서비스수준 분석에 도로용량편람이 제시하는 방법의 적용을 장려하고 있으며 실시간신호제어교차로는 지능형교통체계 구축사업으로 그 숫자가 현장에서 꾸준히 증가하고 있다. 도로용량편람은 정주기식 신호교차로 서비스수준 분석방법에 국한하여 제시하고 있어 현장 실무자들은 실시간신호제어교차로 서비스수준 분석에 어려움을 경험하고 있다. 본 연구는 도로용량편람 수준에서 실시간신호제어교차로 서비스수준 분석의 기초가 되는 방법론을 제안하며 향후 추가로 수행되어야 하는 개발방향을 검토한다. 한 개의 신호교차로를 표본으로 세부 요소모형들을 간이 개발하여 검증한 결과 제안된 방법은 실제 현장 자료와 유사한 수준의 서비스 수준을 추정할 수 있음을 확인하였다.

• International Journal of Precision Engineering and Manufacturing-Green Technology
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• 제5권5호
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• pp.659-670
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• 2018

The Industrial Internet of Things (IIoT) confronts industrial manufactures with economic, ecological, as well as social benefits and challenges, referring to the Triple Bottom Line of sustainability. So far, research has mainly investigated its dimensions in isolation or economic aspects have not been compared with ecological and social perspectives. Further, research misses studies that are devoted to the special characteristics and requirements of Small and Medium-sized Enterprises (SMEs). This study aims to contribute to close this research gap, providing a research context that encompasses all three dimensions of sustainability. The results are based on data obtained from 329 SMEs, 222 in Germany and 107 in China, therefore allowing for a comparison of the concepts "Industrie 4.0" and "Made in China 2025" in the context of SMEs. In general, German SMEs expect a lower impact through "Industrie 4.0", perceiving the concept as more beneficial for larger enterprises. We further find that Chinese SMEs foremost see social benefits. Challenges whilst introducing "Industrie 4.0"by German SMEs as well as several frame conditions are perceived more relevant than for "Made in China 2025", as seen by Chinese SMEs. The paper closes with implications for research and practice based on these findings.