• The Korean Journal of Community Living Science
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• v.16 no.3
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• pp.37-45
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• 2005

This study was conducted to investigate the utilization of cut pear branches for dyeing materials, and the dyeability of pear flowers for pressed flowers using red, orange, and green edible pigments. There was a significant difference of E values among 0.1, 0.5 and $1.0{\%}$ diluted solutions of edible pigments for the cut pear branches coming into flowers. In the higher concentrations of dyeing solutions and the longer dyeing periods, a values with the red pigments increased from 2.37 to 31.22, b values with the orange pigments increased from 7.04 to 45.85, and a - values with the green pigments increased from -3.88 to -36. Bloomed pear branches were immersed in diluted solutions with red, orange and green pigments for forty five minutes. Pear branches immersed in $0.1{\%}$ and $0.5{\%}$ diluted solutions bloomed normally, and each of the flowers were dyed in the colors of the dyeing solutions, respectively. The dyeability of the bloomed pear branches immersed in water and dyeing solutions for one hundred and twenty minutes decreased compared with those treated with red, orange and green pigments and dyed immediately. The dyeability of flowers for the cut pear branches dyed immediately after cutting was significantly increased compared with that immersed in water and dyeing solutions.

• Reproductive and Developmental Biology
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• v.32 no.2
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• pp.135-140
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• 2008

Recent studies on nuclear transfer and induced pluripotent stem cells have demonstrated that differentiated somatic cells can be returned to the undifferentiated state by reversing their developmental process. These epigenetically reprogrammed somatic cells may again be differentiated into various cell types, and used for cell replacement therapies through autologous transplantation to treat many degenerative diseases. To date, however, reprogramming of somatic cells into undifferentiated cells has been extremely inefficient. Hence, reliable markers to identify the event of reprogramming would assist effective selection of reprogrammed cells. In this study, a transgene construct encoding enhanced green fluorescent protein (EGFP) under the regulation of human Oct4 promoter was developed as a reporter for the reprogramming of somatic cells. Microinjection of the transgene construct into pronuclei of fertilized mouse eggs resulted in the emission of green fluorescence, suggesting that the undifferentiated cytoplasmic environment provided by fertilized eggs induces the expression of EGFP. Next, the transgene construct was introduced into human embryonic fibroblasts, and the nuclei from these cells were transferred into enucleated porcine oocytes. Along with their in vitro development, nuclear transfer embryos emitted green fluorescence, suggesting the reprogramming of donor nuclei in nuclear transfer embryos. The results of the present study demonstrate that expression of the transgene under the regulation of human Oct4 promoter coincides with epigenetic reprogramming, and may be used as a convenient marker that non-invasively reflects reprogramming of somatic cells.

• Journal of the Korean Institute of Landscape Architecture
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• v.26 no.2
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• pp.194-206
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• 1998

In 1977, Local governments' planting regulations for the development projects were established in accordance with the revised building law in Korea. A landscaped area, planting densities of trees and shrubs, a percentage of evergreen plants, minimum tree size and species were prescribed in the planting regulations. But the clauses for an excessive planting density and a high ratio of evergreen trees that the regulations includes, have been gradually in the way to a creative planting design, and raise a problem of poor growth of trees an a disordered planting landscape. Therefore, in this study the present planting regulations of 124 local governments throughout the country were analyzed and compared with 13 foreign local governments' of 4 countries ; Japan, the United States of America, Canada and Singapore. And the linitations and characteristics of the regulations are drawn as follows ; 1. The regulations focus on controlling the green spaces and plantings by quantitative methods such as controlling the number of trees and the landscaped area, which are inadequate for estimating the crown coverage of mature trees, and which areinadequate for estimating the crown coverage of mature trees, and implementing the function of polantings and the use of green spaces. 2. Minimums of tree densities are higher than those of foreign countries, especially higher about 10 times to 100 times than those of the United States of America. 3.Excessive number of evergreen trees and fruit trees should be planted under the present planting regulations, that results in constricting the creativity in planting design. 4. An article for using specific tree size, 2.0ms over in height makes it difficult to use a wide variety of different sizes of trees. And there is no incentive measures when larger trees are planted. To enhance the quality of green spaces and plantings, it is needed that the function and locating of green spaces and plantings have to be emphasized, and the planting density should be concerned about the mature tree size. The incentive measure to use various sizes of trees is also needed, and the regulations to use excessive number of evergreen trees or fruit trees should be loosened.

• KIEAE Journal
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• v.13 no.6
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• pp.99-104
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• 2013

Many activities associated with the construction and habitation of buildings are connected with issues affecting the environment such as global warming, climate change, and consumption of valuable natural resources such as fossil fuels. To minimize negative impacts on the environment, the building industry worldwide has implemented green building practices in many countries. One of the main green strategies is to reduce greenhouse gas emissions caused by residential structures because they are most substantially connected with global warming and climate change. To determine the actual quantity of green house gas emissions caused by the construction and use of a building, it is important to analyze total greenhouse gas emissions over the life cycle of buildings including construction, operation & maintenance(O&M) and demolition stages. Many studies suggest methods to calculate greenhouse gas emissions at the construction stage, but the literature addressing greenhouse gas emissions at the O & M stage is limited. A year-long study was conducted utilizing the deterioration method to calculate greenhouse gas emissions at the O & M stage of building life for condominium types of buildings in South Korea. Through this research, it is possible to analyze greenhouse gas emissions of buildings at the O & M stage, the longest span of the life cycle, and eventually help to calculate total greenhouse gas emissions over the life cycle of the building.

• Journal of the Korean Institute of Landscape Architecture
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• v.33 no.2 s.109
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• pp.32-47
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• 2005

This study was carried out to investigate and propose a green-wall system with evergreen plants for urban greening of Tongyeong City. To achieve these goals, the requirements and possibilities for wall greening were investigated and evaluated considering the location, topography, and climate of Tongyeong City. Existing walls were analyzed and then a suitable green wall system is proposed. Tongyeong City and its 151 islands covers the central and the southern parts of the Goseong peninsula. Most of the land is covered with hills and mountains; $43.9\%$ of the land area has a slope greater than $15\%$ and most hills and mountains near the urban area have a slope of more than $30\%$. As a result of the topographical properties, concrete retaining walls can often be seen along the streets in urbanized areas. These retaining walls are not only unattractive, but they also create environmental problems, and thus should be replaced with native evergreen plants. Options for replacing the retaining walls include evergreen vine-plants such as Hedaa spp. and Euonymus radicans, but native evergreen shrubs such as Pittosporum tobira, Nandina domestica, Raphiolepis umbellata, Ilex cornuta, flex crenata, Fatsia japonic, and Aucuba japonica may be a more attractive option. Current wall conditions are unsuitable for planting vines, therefore, a reservoir-drainage-type plant box filled with a light artificial substrate is required for greening these concrete retaining walls. These might be irrigated in the dry season and fertilized annually by an appropriate system. These plant boxes could be attached along the entire walls. An experiment investigating effects of substrates and bark-chip mulching on the growth of Hedera spp. showed that the mixture of cerasoil and field soil(v/v, 4:6) was superior to field soil alone and to the mixture of perlite small grain, large grain, and field soil(v/v/v, 2:2:6). Bark-chip mulching tended to increase the growth of Hedera spp..

• KIEAE Journal
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• v.9 no.2
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• pp.43-52
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• 2009

The aim of this study is to find out the solutions for an improvement of residential environment through the relation between satisfaction of residential environment and amenity awareness of the local residents in Chonnam Mokpo city. For this purpose, it is conducted a questionnaire survey of 203 local residents on the attributes of residential environment. The satisfaction factors of residential environment consist of 8 types of factors such as housing structure, stability, green tract of an avenue, convenience, housing environment, sanitary conditions, waterside facilities, and familiarity. It is analyzed that there is a great difference of the satisfaction between new town dwellers and old town dwellers in three satisfaction factors of a housing structure, convenience, and housing environment. The attributes of amenity awareness consist of 6 types of factors such as fitness, comfort, intimacy, vitality, naturalness, and brilliance. The new town dwellers have a high satisfaction in fitness and vitality factors, and the old town dwellers have a high satisfaction in naturalness factors. Through the analysis to the importance on the satisfaction factors of residential environment related to amenity awareness, it is founded that intimacy factor have importance in order of stability, familiarity, green tract of an avenue and sanitary conditions. Naturalness factor have importance in order of stability, waterside facilities, familiarity and green tract of a avenue. Fitness factor have importance in order of housing structure and sanitary conditions. Comfort factor have importance in order of stability, housing environment and sanitary conditions. And vitality factor have importance in order of housing environment, convenience and green tract of an avenue. Therefore, it is needed that the development plan of residential environment take into consideration in accordance with the importance on the satisfaction of residential environment in Mokpo city.

• Journal of the Korea Society of Computer and Information
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• v.15 no.11
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• pp.83-90
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• 2010

In this paper, we developed the program related green growth based Educational-Robot in the framework of Convergence for the community child welfare centers' children who are in poverty and broken homes as the way to enhance the competitiveness about education and a solution to cope with actively about the main tasks of the state for human resources in the future. Through the program developed, it has been verified the impact on the energy saving attitude. According to this result, it has been proved that convergence study program linked green growth for children of community welfare child center is effective to energy saving attitude. Through it, it is expected to help to meet the educational needs of the community child welfare centers' children.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.2
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• pp.61-72
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• 2009

This study aims to analyze the current situation of and issues in the structure of urban neighborhood parks and park facility installation in cities in Gyeonggi-do. Then, based on its findings, plans to improve the space structure of the parks were examined and suggested to raise the city environment improvement function and green area base function of urban parks and fully satisfy users' needs for pleasant rest, recreation and cultural activities. The findings showed that, within parks, the area of grass where access was restricted reached about 24.4% in average. In other words, only 75.6% of total park area could be freely used by park visitors. As for an improvement plan, the grass area where access is limited may be converted into a gras area that can be freely used to raise the park use area, which currently stands only at 75.6%, to 100%. The result of cross analysis through Chi-Square Test regarding lawn use as well as regarding (dis)agreement with reduction of lawn, based on the investigation of cognition, showed a significant agreement of park users with reduction of green areas. Therefore it is considered that lawn space must be reduced according the result of the analysis, to set up plans for facility space that would meet the desires of users. The analysis of user behaviors revealed that many of them used square spaces and walks; therefore it is considered that re-structuring of space is necessary according to local characteristics. This study also made some policy suggestion. urban parks must be a base of city green areas to counter climate changes. When developing a park creation plan, a user demand survey must be conducted in advance. In addition. grass construction methods should be improved to tolerate foot pressure.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.5
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• pp.81-96
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• 2018

The purpose of this study is to analyze the characteristics of vegetation changes and structures in the buffer green zone of Sihwa Industrial Complex and propose environmental, ecological and multi-functional vegetation management directions. The density of the Pinus thunbergii decreased from $23.1trees/100m^2$ in 2006 to $9.6trees/100m^2$ in 2017 due to the influence of thinning works. Its green volume increased from $0.97m^3/m^2$ to $2.02m^3/m^2$, contributing to the improvement of the atmospheric environment and promotion of biodiversity. The density of deciduous broad-leaved trees at the top maintained at $18{\sim}21trees/100m^2$. In the areas where the understory vegetation was removed, Pueraria lobata spread as an invasive plant and disturbed the forest. In the areas where the understory vegetation was not removed, various native plant species including the Rhus javanica and Celtis sinensis were introduced and contributed to the development of a forest in a multiple structure. There was a concern with the spread of Robinia pseudoacacia which developed into understory vegetation in Pinus thunbergii forest and some canopy forest. The study proposed vegetation management directions for the back, top and front side according to the physical structure of large-scale buffer green zone formed in a long linear form and the usage characteristics of adjacent land.

• Journal of Embryo Transfer
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• v.15 no.2
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• pp.157-165
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• 2000

The efficiency of transgenic livestock production could be improved by early screening of transgene-integration and sexing of embryos at preimplantational stages before trasferring them into recipients. We examined the effciency of multiplex PCR analysis for the simultaneous confirmation of the trasgene and sex during the preimplantational development of bovine embryos and the possibility of green fluorescent protein(GFP) gene as a non-invasive marker for the early screening of transgenic embryos. The GFP gene was microinjected into the male pronuclei of bovine zygotes produced in vitro. The injected zygotes were co-cultured in TCM-199 containing 10% FCS with boving oviductal epithelial cells in a 5% CO2 incubator. Seventeen(13.0%) out of 136 gene-injected bovine zygotes developed by multiplex PCR analysis and the expression of GFP was detected by observing green fluorescence in embryos under a fluorescent microscope. Eight(67%) of 12 embryos at 2-cell to blastocyst stage were positive in the PCR analysis, but only two(11.8%) of 17 blastocysts expressed the GFP gene. Their sex was determined as 7 female and 5 male embryos by the PCR analysis. The results indicate that the screening of GFP gene and sex in bovine embryos by PCR analysis and fluorescence detection could be a promisible method for the preselection of transgenic embryos.