• Journal of Microbiology and Biotechnology
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• v.33 no.6
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• pp.788-796
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• 2023

Canine parvovirus type 2 (CPV-2) has high morbidity and mortality rates in canines. Nonstructural protein 1 (NS1) of CPV-2 has endonuclease activity, initiates viral DNA replication, and is highly conserved. Thus, it is a promising target for antiviral inhibitor development. We overexpressed a 41.9 kDa active recombinant endonuclease in Escherichia coli and designed a nicking assay using carboxyfluorescein and quencher-linked ssDNA as substrates. The optimal temperature and pH of the endonuclease were 37℃ and pH 7, respectively. Curcumin, bisdemethoxycurcumin, demethoxycurcumin, linoleic acid, tannic acid, and α-tocopherol inhibited CPV-2 NS1 endonuclease with IC₅₀ values of 0.29 to 8.03 µM. The extracted turmeric, yerba mate, and sesame cake suppressed CPV-2 NS1 endonuclease with IC₅₀ values of 1.48, 7.09, and 52.67 ㎍/ml, respectively. The binding affinity between curcumin, the strongest inhibitor, and CPV-2 NS1 endonuclease by molecular docking was -6.4 kcal/mol. Curcumin inhibited CPV-2 NS1 endonuclease via numerous hydrophobic interactions and two hydrogen bonds with Lys97 and Pro111 in the allosteric site. These results suggest that adding curcuminoids, linoleic acid, tannic acid, α-tocopherol, extracted turmeric, sesame cake, and yerba to the diet could prevent CPV-2 infection.

• Development and Reproduction
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• v.27 no.2
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• pp.91-99
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• 2023

The sea cucumber, Apostichopus japonicus, is one of the most valuable aquatic species. The color of body wall and appearance are important for the value of sea cucumbers. To examine expression pattern of long-chain acyl-coenzyme A dehydrogenase (LCAD), nuclear distribution C-containing protein 3 (NUDCD3), and receptor tyrosine kinase Tie-1 (TIE1), previously reported as differently expressed genes during the pigmentation of sea cucumber, we analyzed the temporal profiles of LCAD, NUDCD3, and TIE1 mRNAs in LED-exposed and light-shielded A. japonicus. Real-time quantitative PCR revealed that the LCAD, NUDCD3, and TIE1 mRNAs from the juveniles at 40-60 days post-fertilization (dpf) exhibited increasing patterns as compared to those of an early developmental larva (6-dpf). At 60-dpf juveniles, the LCAD and TIE1 mRNA levels of LED-exposed individuals were higher than those of light-shielded ones, whereas at 40-dpf and 50-dpf juveniles, the NUDCD3 mRNA expression was higher in the light-shielded condition (p<0.05). In the pigmented juveniles (90-dpf), the LCAD and TIE1 mRNA levels tended to show higher levels in red individuals than those in green ones, but there was a conversely higher level of NUDCD3 mRNA in green larva. In situ examination of LCAD and NUDCD3 mRNAs in light-shielded 6-dpf larva revealed that both genes are mainly expressed in the internal organs compared to the body surface. Together, these results may provide insights into the differential gene expression of LCAD, NUDCD3, and TIE1 during pigmentation process of the sea cucumber.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.96-96
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• 2022

가상현실 (Virtual Reality, VR) 기술은 3차원 가상공간 내에서의 높은 몰입감에 기반한 체험을 바탕으로 다양한 분야에서 활용되고 있다. 소방훈련이나 태풍, 지진 등 재해 대응훈련과 같이 인명피해의 위험이 있는 재해에 대한 VR 기술을 활용한 방재교육은 위험성을 동반하지 않으면서도 현장감에 기반한 높은 교육적 효과를 창출할 수 있다. 한국전자통신연구원에서는 VR 기술을 이용하여 소방훈련을 위한 실감 소방훈련 시뮬레이터를 개발한 바 있으며 목동재난체험관에서는 홍수, 태풍, 지진 등 다양한 재해에 대한 안전교육을 위한 자연재해 가상현실체험을 운영하고 있다. 이외에도 전국 지자체 및 교육청에서는 방재교육을 목적으로 VR 기술을 활용하고 있다. 그러나 기존의 VR을 활용한 수재해 방재교육은 범람의 수리학적 특성과 함께 수해지의 지형적 특성을 적절히 반영하지 못하는 단점을 가지고 있다. 이는 방재교육이나 경각심을 부각하는 데엔 효과적이나 실질적인 방재 가이드라인을 제시하는 데엔 한계가 있다. 본 연구는 몰입형 파랑해석모형인 Celeris Base를 기반으로 3차원 가상현실 시각화를 활용한 수리학적 홍수추적 모형을 개발하였다. 3차원 가상현실 시각화는 Unity3D를 이용하여 모의환경 내에 구현되었다. 강우-유출 과정의 수리학적 해석을 위해 동수역학 수치모형의 연속방정식 내에 강우와 침투에 대한 항을 추가하였다. 침투모형으로는 Horton 모형, Green-Ampt 모형과 함께 사면의 기울기를 고려한 Green-Ampt 모형을 적용하였다. 실제 유역에서의 홍수추적 모의결과는 관측값과 비교적 잘 일치함을 확인하였다. 개발된 모형은 VR 방재교육을 통해 일반인의 수재해 대응능력 향상에 기여함과 동시에 정확성 높은 홍수추적 모의결과에 기반한 홍수대책 마련에도 활용 가능할 것으로 기대된다.

• Membrane Journal
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• v.33 no.6
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• pp.279-304
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• 2023

Polybenzimidazole (PBI) based membranes have evolved in literature as a popular membrane material for various applications in the past two decades because of their high temperature thermal durability, strong mechanical and tensile properties, high glass transition temperature (T_g), ion conduction ability at elevated temperature (up to 200℃), oxidative or chemical durability along with robust network like structural rigidity, which make PBI membranes suitable for various potential applications in chemically challenging environments. Ion conducting PBI based membranes have been extensively utilized in high temperature proton exchange membrane fuel cells (HT-PEMFC). In addition, PBI based membranes have been vastly utilized for the development of gas separation membranes and organic solvent nanofiltration (OSN) membranes for their unique characteristics. This review will cover the recent progress and application of various types of flat sheet PBI based membranes for HT-PEMFC, gas separation and OSN application.

• Journal of Environmental Science International
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• v.33 no.1
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• pp.9-16
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• 2024

This study analyzed a survey of residents' awareness of rivers and water spaces that reflected residents' awareness in order to establish a basic park green space plan. Through this, the perception and requirements of rivers and water spaces were analyzed and important information was provided for urban planning and environmental management. As a result of analyzing residents' neutral satisfaction with rivers and their opinions on the frequency of use, role, and purpose, it was confirmed that rivers are recognized as an important part of urban life and contain the potential for development for a better urban environment. . However, considering the dissatisfied response and the relatively low frequency of use, we came to the conclusion that better urban planning and management is needed to meet the needs of residents by improving the river environment and providing various facilities. Based on this, city managers should focus on river management and improvement by actively collecting residents' opinions. Through analysis of the causes of dissatisfied responses, measures such as improving the environment around rivers and waste management should be prepared, and a broad strategy for environmental conservation and utilization should be established by reflecting the diverse expectations and demands of residents. These ongoing efforts are expected to have a positive impact on the sustainability of the city and improvement of residents' quality of life. Additionally, the results of this study will be able to contribute to establishing policies for urban planning and environmental improvement through similar research and analysis in other regions.

• Journal of the Korean Institute of Rural Architecture
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• v.26 no.2
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• pp.1-8
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• 2024

Carbon neutrality refers to a state in which there is no global increase in CO₂ emissions due to human activities. In Korea, for carbon neutrality, green remodeling of existing buildings and customized support tasks for zero energy in new buildings are presented. Germany is showing fundamental changes in energy supply, such as applying renewable energy and higher energy efficiency from nuclear and fossil fuels, which were the existing energy sources. In this study, how Germany establishes policies for carbon neutrality at each state level and the cases applied to increase the energy efficiency of the actually applied residential complexes are analyzed based on this. As a result of the case complex analysis, it was found that the construction direction was being promoted as a zero-energy complex or a carbon-neutral complex by gradually reducing the energy demand in buildings and supplying additional energy with new and renewable energy in the low-energy building distribution in the 1990s. In Germany's ecological complex, energy standards have been strengthened from low-energy architecture to plus-energy architecture over time, and annual heating energy consumption standards and heat transmittance rates for each structure have been achieved at a higher level. The results of this analysis will serve as basic data and derivation of applicable items when planning residential complex development and remodeling of existing buildings for the domestic carbon-neutral goal in the future.

• Hwankyungkyoyuk
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• v.19 no.1 s.29
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• pp.1-13
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• 2006

This study was aimed to explore the level of awareness of Korean teachers and college students towards sustainable development(SD) and education for sustainable development(ESD). A survey was conducted to understand the present status of awareness of SD and ESD among 317 college students and 625 teachers in Korea from April to May of 2005. The questionnaire included items asking whether they heard about terms such as sustainability or sustainable development, the source of information on SD, the level of understanding or the urgent task for SD in Korea. It also included questions about experiences in participating in or conducting ESD, the need for ESD, important areas in and the modality for ESD and the willingness to participate in ESD. The results showed that the level of awareness on SD among teachers was low compared to college students, who have a relatively high level of access on SD issues through textbooks and classes in high school. Interestingly, most of college students replied that they never received any ESD, even though they learned SD in class. Both the teacher and student group thought that tile priority of sustainable development should be an 'environment-related' area in the social, economical and environmental perspectives. Most of the students and teachers considered the concept of SD as 'pursuing the balance between environmental protection and economic development.' Some of the teachers recognized the concept of sustainable development in the paradigm of continuous economic development. Both groups responded that the urgent task related to sustainable development is 'environmental protection' and the 'reduction of poverty.' On the other hand, they had experiences in teaching related to natural resources, gender equity, health, human rights, climate changes and other SD issues in class, but not under the name of ESD. They also emphasized 'critical thinking and problem solving & decision-making' in education. Most of the students and teachers responded that it was needed to carry out ESD, and that the way of life for SD would be the priority. It is suggested that various pedagogy and modalities according to various target groups should be considered in providing ESD. It is necessary to use more effective strategies for ESD rather than just introducing the concept of SD. Also, it is needed to review the ESD practices of teachers and improve the quality of education within the scope of ESD.

• Korean journal of applied entomology
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• v.47 no.3
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• pp.249-254
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• 2008

This study was conducted to develop an organic control method of two spotted spider mite(Tetranychus urticae) by using cooking oil and yolk mixture(COY) through evaluating its acaricidal activity in laboratory and green house. In laboratory, there is no significant difference in acaricidal activity against T. urticae within the COY including soybean, canola(rape seed), sunflower and olive oil. The acaricidal activity against T. urticae. increased from 17.6% to 94.1% as the COY became concentrated between 0.1% to 1%. The COY acricidal activity was effected by the quantity of treatment according to application methods. The COY dealt with T. urticae eggs presented 95% of the ovicidal activity. In rose greenhouse damaged by T. urticae, the COY(0.3%) was sprayed three times and resulted in the high control value of mites between 69.0% to 89.6%.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.3
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• pp.358-363
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• 2008

The intracytoplasmic sperm injection (ICSI) procedure has recently been utilized to produce transgenic animals and may serve as an alternative to the conventional pronuclear microinjection in species such as pigs whose ooplasm is opaque and pronuclei are often invisible. In this study, the effects of sperm membrane disruption and electrical activation of oocytes on in vitro development and expression of transgene green fluorescent protein (GFP) in ICSI embryos were tested to refine this recently developed procedure. Prior to ICSI, sperm heads were treated with Triton X-100+NaCl or Triton X-100+NaCl+NaOH, to disrupt membrane to be permeable to exogenous DNA, and incubated with linearized pEGFP-N1 vector. To induce activation of oocytes, a single DC pulse of 1.3 kV/cm was applied to oocytes for $30{\mu}sec$. After ICSI was performed with the aid of a micromanipulator, in vitro development of embryos and GFP expression were monitored. The chemical treatment to disrupt sperm membrane did not affect the developmental competence of embryos. 40 to 60% of oocytes were cleaved after injection of sperm heads with disrupted membrane, whereas 48.6% (34/70) were cleaved without chemical treatment. Regardless of electrical stimulation to induce activation, oocytes were cleaved after ICSI, reflecting that, despite sperm membrane disruption, the perinuclear soluble sperm factor known to mediate oocyte activation remained intact. After development to the 4-cell stage, 11.8 (2/17, Triton X-100+NaCl+NaOH) to 58.8% (10/17, Triton X-100+NaCl) of embryos expressed GFP. The expression of GFP beyond the stage of embryonic genome activation (4-cell stage in the pig) indicates that the exogenous DNA might have been integrated into the porcine genome. When sperm heads were co-incubated with exogenous DNA following the treatment of Triton X-100+NaCl, GFP expression was observed in high percentage (58.8%) of embryos, suggesting that transgenic pigs may efficiently be produced using ICSI.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.1
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• pp.35-42
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• 2011

The current study analyzed appropriateness of abandoned rice paddy wetlands as habitats for the Amphibia by assessing functions and value of abandoned rice paddy wetlands within land development districts as a habitat for living creatures and researching into the Amphibia living in the subject districts. For this purpose, the study designated abandoned rice paddy wetlands within Hwaseong A District and Pangyo B District under land development as subject lands. Those wetlands went through succession after abandonment of farming. Detailed study results are as follows. From classification of wetland types and an analysis of their characteristics, it was found that herbaceous plants such as smartweed communities, horsetail communities and reed communities, and woody plants such as pussy willow communities appeared in both districts. These abandoned rice paddy wetlands shows typical characteristics of palustrine wetlands. As a result of a vegetation structure research, it was observed that succession has been progressed for at least over 2~3 years. In assessment of wetland functions by item, it was found that they are developing into fine wetlands in terms of functions because they are ranked over Moderate Class except separate distance from streams. Also from a research into animals living in the abandoned rice paddy wetlands, it was found that main inhabitants are Rana nigromaculata,Rana dybowskii, Hyla japonica, Bufo bufo gargarizans, etc. It can be confirmed that the abandoned rice paddy wetlands are providing appropriate conditions as habitats to the Amphibia in a view that those species have life cycles of inhabitation (shores and wetlands) - egg-laying (rice paddy, puddles and swampy places) - hibernation (rice field banks and swampy places). From this point of view, it was found that abandoned rice paddy wetlands that developed because of abandonment of farming can be used as means to improve structure and functions of habitats.