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Field Studios of In-situ Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbons

  • Semprini, Lewts
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.3-4
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    • 2004
  • Results will be presented from two field studies that evaluated the in-situ treatment of chlorinated aliphatic hydrocarbons (CAHs) using aerobic cometabolism. In the first study, a cometabolic air sparging (CAS) demonstration was conducted at McClellan Air Force Base (AFB), California, to treat chlorinated aliphatic hydrocarbons (CAHs) in groundwater using propane as the cometabolic substrate. A propane-biostimulated zone was sparged with a propane/air mixture and a control zone was sparged with air alone. Propane-utilizers were effectively stimulated in the saturated zone with repeated intermediate sparging of propane and air. Propane delivery, however, was not uniform, with propane mainly observed in down-gradient observation wells. Trichloroethene (TCE), cis-1, 2-dichloroethene (c-DCE), and dissolved oxygen (DO) concentration levels decreased in proportion with propane usage, with c-DCE decreasing more rapidly than TCE. The more rapid removal of c-DCE indicated biotransformation and not just physical removal by stripping. Propane utilization rates and rates of CAH removal slowed after three to four months of repeated propane additions, which coincided with tile depletion of nitrogen (as nitrate). Ammonia was then added to the propane/air mixture as a nitrogen source. After a six-month period between propane additions, rapid propane-utilization was observed. Nitrate was present due to groundwater flow into the treatment zone and/or by the oxidation of tile previously injected ammonia. In the propane-stimulated zone, c-DCE concentrations decreased below tile detection limit (1 $\mu$g/L), and TCE concentrations ranged from less than 5 $\mu$g/L to 30 $\mu$g/L, representing removals of 90 to 97%. In the air sparged control zone, TCE was removed at only two monitoring locations nearest the sparge-well, to concentrations of 15 $\mu$g/L and 60 $\mu$g/L. The responses indicate that stripping as well as biological treatment were responsible for the removal of contaminants in the biostimulated zone, with biostimulation enhancing removals to lower contaminant levels. As part of that study bacterial population shifts that occurred in the groundwater during CAS and air sparging control were evaluated by length heterogeneity polymerase chain reaction (LH-PCR) fragment analysis. The results showed that an organism(5) that had a fragment size of 385 base pairs (385 bp) was positively correlated with propane removal rates. The 385 bp fragment consisted of up to 83% of the total fragments in the analysis when propane removal rates peaked. A 16S rRNA clone library made from the bacteria sampled in propane sparged groundwater included clones of a TM7 division bacterium that had a 385bp LH-PCR fragment; no other bacterial species with this fragment size were detected. Both propane removal rates and the 385bp LH-PCR fragment decreased as nitrate levels in the groundwater decreased. In the second study the potential for bioaugmentation of a butane culture was evaluated in a series of field tests conducted at the Moffett Field Air Station in California. A butane-utilizing mixed culture that was effective in transforming 1, 1-dichloroethene (1, 1-DCE), 1, 1, 1-trichloroethane (1, 1, 1-TCA), and 1, 1-dichloroethane (1, 1-DCA) was added to the saturated zone at the test site. This mixture of contaminants was evaluated since they are often present as together as the result of 1, 1, 1-TCA contamination and the abiotic and biotic transformation of 1, 1, 1-TCA to 1, 1-DCE and 1, 1-DCA. Model simulations were performed prior to the initiation of the field study. The simulations were performed with a transport code that included processes for in-situ cometabolism, including microbial growth and decay, substrate and oxygen utilization, and the cometabolism of dual contaminants (1, 1-DCE and 1, 1, 1-TCA). Based on the results of detailed kinetic studies with the culture, cometabolic transformation kinetics were incorporated that butane mixed-inhibition on 1, 1-DCE and 1, 1, 1-TCA transformation, and competitive inhibition of 1, 1-DCE and 1, 1, 1-TCA on butane utilization. A transformation capacity term was also included in the model formation that results in cell loss due to contaminant transformation. Parameters for the model simulations were determined independently in kinetic studies with the butane-utilizing culture and through batch microcosm tests with groundwater and aquifer solids from the field test zone with the butane-utilizing culture added. In microcosm tests, the model simulated well the repetitive utilization of butane and cometabolism of 1.1, 1-TCA and 1, 1-DCE, as well as the transformation of 1, 1-DCE as it was repeatedly transformed at increased aqueous concentrations. Model simulations were then performed under the transport conditions of the field test to explore the effects of the bioaugmentation dose and the response of the system to tile biostimulation with alternating pulses of dissolved butane and oxygen in the presence of 1, 1-DCE (50 $\mu$g/L) and 1, 1, 1-TCA (250 $\mu$g/L). A uniform aquifer bioaugmentation dose of 0.5 mg/L of cells resulted in complete utilization of the butane 2-meters downgradient of the injection well within 200-hrs of bioaugmentation and butane addition. 1, 1-DCE was much more rapidly transformed than 1, 1, 1-TCA, and efficient 1, 1, 1-TCA removal occurred only after 1, 1-DCE and butane were decreased in concentration. The simulations demonstrated the strong inhibition of both 1, 1-DCE and butane on 1, 1, 1-TCA transformation, and the more rapid 1, 1-DCE transformation kinetics. Results of tile field demonstration indicated that bioaugmentation was successfully implemented; however it was difficult to maintain effective treatment for long periods of time (50 days or more). The demonstration showed that the bioaugmented experimental leg effectively transformed 1, 1-DCE and 1, 1-DCA, and was somewhat effective in transforming 1, 1, 1-TCA. The indigenous experimental leg treated in the same way as the bioaugmented leg was much less effective in treating the contaminant mixture. The best operating performance was achieved in the bioaugmented leg with about over 90%, 80%, 60 % removal for 1, 1-DCE, 1, 1-DCA, and 1, 1, 1-TCA, respectively. Molecular methods were used to track and enumerate the bioaugmented culture in the test zone. Real Time PCR analysis was used to on enumerate the bioaugmented culture. The results show higher numbers of the bioaugmented microorganisms were present in the treatment zone groundwater when the contaminants were being effective transformed. A decrease in these numbers was associated with a reduction in treatment performance. The results of the field tests indicated that although bioaugmentation can be successfully implemented, competition for the growth substrate (butane) by the indigenous microorganisms likely lead to the decrease in long-term performance.

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Sentiment Analysis of Movie Review Using Integrated CNN-LSTM Mode (CNN-LSTM 조합모델을 이용한 영화리뷰 감성분석)

  • Park, Ho-yeon;Kim, Kyoung-jae
    • Journal of Intelligence and Information Systems
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    • v.25 no.4
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    • pp.141-154
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    • 2019
  • Rapid growth of internet technology and social media is progressing. Data mining technology has evolved to enable unstructured document representations in a variety of applications. Sentiment analysis is an important technology that can distinguish poor or high-quality content through text data of products, and it has proliferated during text mining. Sentiment analysis mainly analyzes people's opinions in text data by assigning predefined data categories as positive and negative. This has been studied in various directions in terms of accuracy from simple rule-based to dictionary-based approaches using predefined labels. In fact, sentiment analysis is one of the most active researches in natural language processing and is widely studied in text mining. When real online reviews aren't available for others, it's not only easy to openly collect information, but it also affects your business. In marketing, real-world information from customers is gathered on websites, not surveys. Depending on whether the website's posts are positive or negative, the customer response is reflected in the sales and tries to identify the information. However, many reviews on a website are not always good, and difficult to identify. The earlier studies in this research area used the reviews data of the Amazon.com shopping mal, but the research data used in the recent studies uses the data for stock market trends, blogs, news articles, weather forecasts, IMDB, and facebook etc. However, the lack of accuracy is recognized because sentiment calculations are changed according to the subject, paragraph, sentiment lexicon direction, and sentence strength. This study aims to classify the polarity analysis of sentiment analysis into positive and negative categories and increase the prediction accuracy of the polarity analysis using the pretrained IMDB review data set. First, the text classification algorithm related to sentiment analysis adopts the popular machine learning algorithms such as NB (naive bayes), SVM (support vector machines), XGboost, RF (random forests), and Gradient Boost as comparative models. Second, deep learning has demonstrated discriminative features that can extract complex features of data. Representative algorithms are CNN (convolution neural networks), RNN (recurrent neural networks), LSTM (long-short term memory). CNN can be used similarly to BoW when processing a sentence in vector format, but does not consider sequential data attributes. RNN can handle well in order because it takes into account the time information of the data, but there is a long-term dependency on memory. To solve the problem of long-term dependence, LSTM is used. For the comparison, CNN and LSTM were chosen as simple deep learning models. In addition to classical machine learning algorithms, CNN, LSTM, and the integrated models were analyzed. Although there are many parameters for the algorithms, we examined the relationship between numerical value and precision to find the optimal combination. And, we tried to figure out how the models work well for sentiment analysis and how these models work. This study proposes integrated CNN and LSTM algorithms to extract the positive and negative features of text analysis. The reasons for mixing these two algorithms are as follows. CNN can extract features for the classification automatically by applying convolution layer and massively parallel processing. LSTM is not capable of highly parallel processing. Like faucets, the LSTM has input, output, and forget gates that can be moved and controlled at a desired time. These gates have the advantage of placing memory blocks on hidden nodes. The memory block of the LSTM may not store all the data, but it can solve the CNN's long-term dependency problem. Furthermore, when LSTM is used in CNN's pooling layer, it has an end-to-end structure, so that spatial and temporal features can be designed simultaneously. In combination with CNN-LSTM, 90.33% accuracy was measured. This is slower than CNN, but faster than LSTM. The presented model was more accurate than other models. In addition, each word embedding layer can be improved when training the kernel step by step. CNN-LSTM can improve the weakness of each model, and there is an advantage of improving the learning by layer using the end-to-end structure of LSTM. Based on these reasons, this study tries to enhance the classification accuracy of movie reviews using the integrated CNN-LSTM model.

Estimation of Addition and Removal Processes of Nutrients from Bottom Water in the Saemangeum Salt-Water Lake by Using Mixing Model (혼합모델을 이용한 새만금호 저층수 내 영양염의 공급과 제거에 관한 연구)

  • Jeong, Yong Hoon;Kim, Chang Shik;Yang, Jae Sam
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.17 no.4
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    • pp.306-317
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    • 2014
  • This study has been executed to understand the additional and removal processes of nutrients in the Saemangeum Salt-water Lake, and discussed with other monthly-collected environmental parameters such as water temperature, salinity, dissolved oxygen, suspended solids, and Chl-a from 2008 to 2010. $NO_3$-N, TP, $PO_4$-P, and DISi showed the removal processes along with the salinity gradients at the surface water of the lake, whereas $NO_2$-N, $NH_4$-N, and Chl-a showed addition trend. In the bottom water all water quality parameters except $NO_3$-N appeared addition processes indicating evidence of continuous nutrients suppliance into the bottom layer. The mixing modelling approach revealed that the biogeochemical processes in the lake consume $NO_3$-N and consequently added $NH_4$-N and $PO_4$-P to the bottom water during the summer seasons. The $NH_4$-N and $PO_4$-P appeared strong increase at the bottom water of the river-side of the lake and strong concentration gradient difference of dissolved oxygen also appeared in the same time. DISi exhibited continuous seasonal supply from spring to summer. Internal addition of $NH_4$-N and $PO_4$-P in the river-side of the lake were much higher than the dike-side, while the increase of DISi showed similar level both the dike and river sides. The temporal distribution of benthic flux for DISi indicates that addition of nutrients in the bottom water was strongly affected by other sources, for example, submarine ground-water discharge (SGD) through bottom sediment.

Development of 2.5D Electron Dose Calculation Algorithm (2.5D 전자선 선량계산 알고리즘 개발)

  • 조병철;고영은;오도훈;배훈식
    • Progress in Medical Physics
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    • v.10 no.3
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    • pp.133-140
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    • 1999
  • In this paper, as a preliminary study for developing a full 3D electron dose calculation algorithm, We developed 2.5D electron dose calculation algorithm by extending 2D pencil-beam model to consider three dimensional geometry such as air-gap and obliquity appropriately. The dose calculation algorithm was implemented using the IDL5.2(Research Systems Inc., USA), For calculation of the Hogstrom's pencil-beam algorithm, the measured data of the central-axis depth-dose for 12 MeV(Siemens M6740) and the linear stopping power and the linear scattering power of water and air from ICRU report 35 was used. To evaluate the accuracy of the implemented program, we compared the calculated dose distribution with the film measurements in the three situations; the normal incident beam, the 45$^{\circ}$ oblique incident beam, and the beam incident on the pit-shaped phantom. As results, about 120 seconds had been required on the PC (Pentium III 450MHz) to calculate dose distribution of a single beam. It needs some optimizing methods to speed up the dose calculation. For the accuracy of dose calculation, in the case of the normal incident beam of the regular and irregular shaped field, at the rapid dose gradient region of penumbra, the errors were within $\pm$3 mm and the dose profiles were agreed within 5%. However, the discrepancy between the calculation and the measurement were about 10% for the oblique incident beam and the beam incident on the pit-shaped phantom. In conclusions, we expended 2D pencil-beam algorithm to take into account the three dimensional geometry of the patient. And also, as well as the dose calculation of irregular field, the irregular shaped body contour and the air-gap could be considered appropriately in the implemented program. In the near future, the more accurate algorithm will be implemented considering inhomogeneity correction using CT, and at that time, the program can be used as a tool for educational and research purpose. This study was supported by a grant (#HMP-98-G-1-016) of the HAN(Highly Advanced National) Project, Ministry of Health & Welfare, R.O.K.

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Estimation of Soil Loss Due to Cropland Increase in Hoeryeung, Northeast Korea (북한 회령지역의 농경지 변화에 따른 토양침식 추정)

  • Lee, Min-Boo;Kim, Nam-Shin;Kang, Chul-Sung;Shin, Keun-Ha;Choe, Han-Sung;Han, Uk
    • Journal of the Korean association of regional geographers
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    • v.9 no.3
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    • pp.373-384
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    • 2003
  • This study analyses the soil loss due to cropland increase in the Hoeryeung area of northeast Korea, using Landsat images of 1987 TM and 2001 ETM, together with DTED, soil and geological maps, and rainfall data of 20 years. Items of land cover and land use were categorized as cropland, settlement, forest, river zone, and sand deposit by supervised classification with spectral bands 1, 2 and 3. RUSLE model is used for estimation of soil loss, and AML language for calculation of soil loss volumes. Fourier transformation method is used for unification of the geographical grids between Landsat images and DTED. GTD was selected from 1:50,000 topographic map. Main sources of soil losses over 100 ton/year may be the river zone and settlement in the both times of 1987 and 2001, but the image of the 2001 shows that sources areas have developed up to the higher mountain slopes. In the cropland average, increases of hight and gradient are 24m and $0.8^{\circ}$ from 1987 to 2001. In the case of new developed cropland, average increases are 75m and $2.5^{\circ}$, and highest soil loss has occurred at the elevation between 300 and 500m. The soil loss 57 ton of 1987 year increased 85 ton of 2001 year. Soil loss is highest in $30{\sim}50^{\circ}$ slope zones in both years, but in 2001 year, soil loss increased under $30^{\circ}$ zones. The size of area over 200 ton/year, indicating higher risk of landslides, have increased from $28.6km^2$ of 1987 year to $48.8km^2$ of 2001 year.

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Interannual and Seasonal Variations of Water Quality in Terms of Size Dimension on Multi-Purpose Korean Dam Reservoirs Along with the Characteristics of Longitudinal Gradients (우리나라 다목적댐 인공호들의 규모에 따른 연별.계절별 수질변이 및 상.하류간 종적구배 특성)

  • Han, Jeong-Ho;Lee, Ji-Yeoun;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.43 no.2
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    • pp.319-337
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    • 2010
  • Major objective of this study was to determine interannual and seasonal water quality along with characteristics of longitudinal gradients along the reservoir axis of the riverine zone (Rz)-to-lacustrine zone (Lz). Water quality dataset of five years during 2003~2007 used here were obtained from Ministry of Environment, Korea and ten physical, chemical and biological parameters were analyzed in the study. Similarity analysis, based on moropho-hydrological variables of reservoir surface area, watershed area, total inflow, and outflow, showed that the reservoirs were categorized as three groups of large-dam reservoirs (Chungju Reservoir, Daecheong Reservoir and Soyang Reservoir), mid-size reservoirs (Andong Reservoir, Yongdam Reservoir, Juam Reservoir and Hapcheon Reservoir), and small-size reservoirs (Hoengseong Reservoir and Buan Reservoir). According to the data comparison of high-flow year (2003) vs. lowflow year (2005), dissolved oxygen (DO), pH, biological oxygen demand (BOD), suspended solids (SS), total nitrogen (TN), total phosphorus (TP), chlorophyll-a (CHL) and electrical conductivity (EC) declined along the longitudinal axis of Rz to Lz and water transparency, based on Secchi depth (SD), increased along the axis. These results indicate that transparency was a function of Values of pH, DO, SS, SD, and EC at each site were greater in the low-flow year (2005) than the high-flow year (2003), whereas values of BOD, COD, TN, TP and CHL were greater in the high-flow year (2003). When values of TN, TP, CHL and SD in nine reservoirs were compared in the three zones of Rz, Tz, and Lz, values of TN, TP and CHL declined along longitudinal gradients and SD showed the opposite due to the sedimentation processes from the water column. Values of TN were not statistically correlated with TP values. The empirical linear models of TP-CHL and CHL-SD showed significant (p<0.05, $R^2$>0.04). In the mid-size reservoirs, the variation of CHL was explained ($R^2$=0.2401, p<0.0001, n=239) by the variation of TP. The affinities in the correlation analysis of mid-size reservoirs were greater in the CHL-SD model than any other empirical models, and the CHL-SD model had an inverse relations. In the meantime, water quality variations was evidently greater in Daecheong Reservoir than two reservoirs of Andong Reservoir and Hoengseong Reservoir as a result of large differences of water quality by long distance among Rz, Tz and Lz.

Spatial Patterns and Temporal Variability of the Haines Index related to the Wildland Fire Growth Potential over the Korean Peninsula (한반도 산불 확장 잠재도와 관련된 Haines Index의 시.공간적 특징)

  • Choi Cwang-Yong;Kim Jun-Su;Won Myoung-Soo
    • Journal of the Korean Geographical Society
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    • v.41 no.2 s.113
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    • pp.168-187
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    • 2006
  • Windy meteorological conditions and dried fire fuels due to higher atmospheric instability and dryness in the lower troposphere can exacerbate fire controls and result in more losses of forest resources and residential properties due to enhanced large wildland fires. Long-term (1979-2005) climatology of the Haines Index reconstructed in this study reveals that spatial patterns and intra-annual variability of the atmospheric instability and dryness in the lower troposphere affect the frequency of wildland fire incidences over the Korean Peninsula. Exponential regression models verify that daily high Haines Index and its monthly frequency has statistically significant correlations with the frequency of the wildland fire occurrences during the fire season (December-April) in South Korea. According to the climatic maps of the Haines Index created by the Geographic Information System (GIS) using the Digital Elevation Model (DEM), the lowlands below 500m from the mean sea level in the northwestern regions of the Korean Peninsula demonstrates the high frequency of the Haines Index equal to or greater than five in April and May. The annual frequency of the high Haines Index represents an increasing trend across the Korean Peninsula since the mid-1990s, particularly in Gyeongsangbuk-do and along the eastern coastal areas. The composite of synoptic weather maps at 500hPa for extreme events, in which the high Haines Index lasted for several days consecutively, illustrates that the cold low pressure system developed around the Sea of Okhotsk in the extreme event period enhances the pressure gradient and westerly wind speed over the Korean Peninsula. These results demonstrate the need for further consideration of the spatial-temporal characteristics of vertical atmospheric components, such as atmospheric instability and dryness, in the current Korean fire prediction system.

Serial MR Imaging of Magnetically Labeled Humen Umbilical Vein Endothelial Cells in Acute Renal Failure Rat Model (급성 신부전 쥐 모델에서 자기 표지된 인간 제대정맥 내피세포의 연속 자기공명영상)

  • Lee, Sun Joo;Lee, Sang Yong;Kang, Kyung Pyo;Kim, Won;Park, Sung Kwang
    • Investigative Magnetic Resonance Imaging
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    • v.17 no.3
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    • pp.181-191
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    • 2013
  • Purpose : To evaluate the usefulness of in vivo magnetic resonance (MR) imaging for tracking intravenously injected superparamagnetic iron oxide (SPIO)-labeled human umbilical vein endothelial cells (HUVECs) in an acute renal failure (ARF) rat model. Materials and Methods: HUVECs were labeled with SPIO and poly-L-lysine (PLL) complex. Relaxation rates at 1.5-T MR, cell viability, and labeling stability were assessed. HUVECs were injected into the tail vein of ARF rats (labeled cells in 10 rats, unlabeled cells in 2 rats). Follow-up serial $T2^*$-weighted gradient-echo MR imaging was performed at 1, 3, 5 and 7 days after injection, and the MR findings were compared with histologic findings. Results: There was an average of $98.4{\pm}2.4%$ Prussian blue stain-positive cells after labeling with SPIOPLL complex. Relaxation rates ($R2^*$) of all cultured HUVECs at day 3 and 5 were not markedly decreased compared with that at day 1. The stability of SPIO in HUVECs was maintained during the proliferation of HUVECs in culture media. In the presence of left unilateral renal artery ischemia, $T2^*$-weighted MR imaging performed 1 day after the intravenous injection of labeled HUVECs revealed a significant signal intensity (SI) loss exclusively in the left renal outer medulla regions, but not in the right kidney. The MR imaging findings at days 3, 5 and 7 after intravenous injection of HUVECs showed a SI loss in the outer medulla regions of the ischemically injured kidney, but the SI progressively recovered with time and the right kidney did not have a significant change in SI in the same period. Upon histologic analysis, the SI loss on MR images was correspondent to the presence of Prussian blue stained cells, primarily in the renal outer medulla. Conclusion: MR imaging appears to be useful for in vivo monitoring of intravenously injected SPIO-labeled HUVECs in an ischemically injured rat kidney.

Three-Dimensional High-Frequency Electromagnetic Modeling Using Vector Finite Elements (벡터 유한 요소를 이용한 고주파 3차원 전자탐사 모델링)

  • Son Jeong-Sul;Song Yoonho;Chung Seung-Hwan;Suh Jung Hee
    • Geophysics and Geophysical Exploration
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    • v.5 no.4
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    • pp.280-290
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    • 2002
  • Three-dimensional (3-D) electromagnetic (EM) modeling algorithm has been developed using finite element method (FEM) to acquire more efficient interpretation techniques of EM data. When FEM based on nodal elements is applied to EM problem, spurious solutions, so called 'vector parasite', are occurred due to the discontinuity of normal electric fields and may lead the completely erroneous results. Among the methods curing the spurious problem, this study adopts vector element of which basis function has the amplitude and direction. To reduce computational cost and required core memory, complex bi-conjugate gradient (CBCG) method is applied to solving complex symmetric matrix of FEM and point Jacobi method is used to accelerate convergence rate. To verify the developed 3-D EM modeling algorithm, its electric and magnetic field for a layered-earth model are compared with those of layered-earth solution. As we expected, the vector based FEM developed in this study does not cause ny vector parasite problem, while conventional nodal based FEM causes lots of errors due to the discontinuity of field variables. For testing the applicability to high frequencies 100 MHz is used as an operating frequency for the layer structure. Modeled fields calculated from developed code are also well matched with the layered-earth ones for a model with dielectric anomaly as well as conductive anomaly. In a vertical electric dipole source case, however, the discontinuity of field variables causes the conventional nodal based FEM to include a lot of errors due to the vector parasite. Even for the case, the vector based FEM gave almost the same results as the layered-earth solution. The magnetic fields induced by a dielectric anomaly at high frequencies show unique behaviors different from those by a conductive anomaly. Since our 3-D EM modeling code can reflect the effect from a dielectric anomaly as well as a conductive anomaly, it may be a groundwork not only to apply high frequency EM method to the field survey but also to analyze the fold data obtained by high frequency EM method.

The Comparative Imaging Study on Mn-phthalocyanine and Mangafodipir trisodium in Experimental VX2 Animal Model (실험적으로 유발시킨 VX2 동물모델에서의 Mn-phthalocyanine과 Mangafodipir trisodium의 비교영상)

  • Park Hyun-Jeong;Ko Sung-Min;Kim Yong-Sun;Chang Yongmin
    • Investigative Magnetic Resonance Imaging
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    • v.8 no.1
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    • pp.32-41
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    • 2004
  • Purpose : To measure the NMR relaxation properties of MnPC, to observe the characteristics of liver enhancement patterns on MR images in experimentally implanted rabbit VX2 tumor model, and to estimate the possibility of tissue specific contrast agent for MnPC in comparison with the hepatobiliary agent. Materials and Methods : Phthalocyanine (PC) was chelated with paramagnetic ions, manganese (Mn). 2.01 g (5.2 mmol) of phthalocyanine was mixed with 0.37 g (1.4 nlmol) of Mn chloride at $310^{\circ}C$ for 36 hours and then purified by chromatography ($CHCl_3:\;CH_3OH=98:2$, volume ratio) to obtain 1.04 g $(46\%)$ of MnPC (molecular weight = 2000 daltons). The T1/T2 relaxivity (R1/R2) for MnPC were determined at a 1.5 T (64 MHz) MR spectrometer. VX2 tumor model was experimentally implanted in the liver parenchyma of rabbits. All MR studies were performed on 1.5 T. The human extremity radio frequency coil of a bird cage type was employed. MR images were acquired at 17 to 24 days after VX2 carcinoma implantation.4 mmol/kg MnPC and 0.01 mmol/kg Mn-DPDP were injected via the ear vein of rabbits. T1-weighted images were obtained with spin-echo (TR/TE=516/14 msec) and fast multiplanar spoiled gradient recalled (TR/TE : 80/4 msec, $60^{\circ}$ flip angle) pulse sequence. Fast spin-echo (TR/TE=1200/85 msec) was used to obtain the T2-weighted images. Results : The value of T1/T2 relaxivity (R1/R2) of MnPC was $7.28\;mM^{-1}S^{-1}$ and $55.56\;mM^{-1}S^{-1}$ respectively at 1.5 T (64 MHz). Because the T2 relaxivity of MnPC that bonded strongly, covalently manganese with phthalocyanine was very high, the signal intensity of liver parenchyma was decreased on postcontrast T2-weighted images and we could easily distinguish the VX2 carcinoma within the liver parenchyma. When MnPC was administrated intravenously, the tumor margin delineation was more remarkable than Mn-DPDP-enhanced images. The enhancement of liver parenchyma with MnPC persisted at relatively high levels over at least one hour after injection of the contrast agents. Conclusion : The hepatic uptake and biliary excretion of MnPC, which are similar to Mn-DPDP, suggest that this agent is a new liver-specific agent. Also, MnPC seems to be used as a dual contrast agent (T1 and T2) with high T2 relaxivity. However, it is warranted that MnPC needs further investigation as a potential contrast agent for MR imaging of the liver. That is, further characterizations of MnPC are needed in vivo and in vitro before clinical trials. The diagnostic potential of MnPC will also have to be examined more in the animal models of additional types.

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