• Fisheries and Aquatic Sciences
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• 제5권4호
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• pp.308-310
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• 2002

Effect of 4-nonylphenol (4-NP), endocrine disrupting compounds (EDCs), on glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) were investigated in the plasma of juvenile rockfish, Sebastes schlegeli. Fish were injected with 4­NP (10, 50, 100 and 200 mg/kg body weight) in $70\%$ ethanol twice at 3-day intervals and plasma sampling were extracted at 7 days after the last injection. Controls received solvent only. 4-NP significant increased GOT in a dose-dependent manner. GPT was markedly elevated to $61\%$ (P<0.05) and $82\%$ (P<0.01) than that of the control at the 4-NP doses of 100 and 200mg, respectively. These results suggest that the estrogenic activity of 4-NP increase plasma GOT and GPT by toxic effect on hepatocyte.

• Environmental Analysis Health and Toxicology
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• 제17권3호
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• pp.239-243
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• 2002

Changes of plasma vitellogenin (VTG) and glutamate pyruvate transaminase (GPT) were examined for determining whether hepatocyte was damaged during the process of VTG induction in the juvenile rockfish, Sebastes schlegeli exposed to exogenous estrogen (estradiol-l7$\beta$, E$_2$). Rockfishes were intraperitoneally injected with E$_2$(5 mg/kg B.W.) in 70% ethanol and plasma sampling were extracted at 0, 1, 3, 6, 9, 12, 15 days af-ter E$_2$administration. VTG and GPT were then analyzed by SDS -PAGE and Reitman -Frankel method, respectively. VTG band was detected at a molecular weight position of 175 kDa on Day 3 after E$_2$administration. This band became more distinct at 6 days, but its was gradually thinned with time -course, and not detected at 15 days. GPT was suddenly increased at 1 days after 22 administration and highest GPT was detected at 3 days. However. GPT was gradually decreased with time -course as the change of VTG. These results suggest that the process of VTG induction by exogenous E$_2$damage to hepatocyte, and plasma GPT was temporarily increased in the juvenile rockfish.

• 한국식품영양과학회지
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• 제29권2호
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• pp.193-197
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• 2000

The effects of black soybena extracts on enzymes activies of rat were evaluated in present study. Eighty-four male Sprague-Dawley rats weighing 100$\pm$10g were divided into twelve groups which consisted of black soybean extract, Pb and Cd solution, and black soybean extract plus Pb or Cd soln groups. The weight gain was increased in black soybean extracts and Pb soln solution group but decreased in Cd soln solution group. The results obtained form the experiment were as follows: Glutamate pyruvate trasaminase (GPT) and glutamate oxaloacetate oxaloacetate transaminase (GOT) activities were not significantly different among experimental groups. The lactate dehydrogenase (LDH) activities of black soybean extract administered groups were decreased than those of Pb and Cd solution group. Black soybean group increased cholinesterase (ChEase) activity as compared to administration of Pb and Cd soln group.

• 한국어병학회지
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• 제17권3호
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• pp.191-198
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• 2004

$estradiol-17\beta$ ${E_2}$에 노출된 미성숙 조피볼락의 혈장 내 vitellogenin (VTG), alkaline-labile protein phosphorus (ALPP), calcium (Ca), glutamate pyruvate transaminase (GPT) 및 hepatosomatic index (HSI)의 일시적인 변화가 조사되었다. 70% 에탄올에 녹여진 ${E_2}$ (5 mg/kg B.W.)를 조피볼락 치어의 복강에 주사한 후 0, 1, 3, 6, 9, 12 및 15일에 혈장이 수집되었다. 전기 영동상에서 주사 후, 3일째 약 170 kDa의 위치에 염색성을 나타내는 VTG 밴드가 검출되었다. 밴드의 염색성은 6일째 더욱 강하게 나타났지만, 시간의 경과와 더불어 흐려져 15일째에는 노출 전 처럼 VTG 밴드가 관찰되지 않았다. 혈장 내 ALPP와 Ca는 1일째부터 급격히 증가했으며 VTG 변동과 유사하게 6일째 가장 높은 농도를 나타낸 후, 시간의 경과와 더불어 점점 감소해 15일째는 노출 전과 유사한 값을 나타내었다. GPT는 투여 1일부터 급격히 증가해 3일에 최고치를 나타낸 이후 시간의 경과와 더불어 감소하는 경향을 나타냈으며 HSI의 경우도 1일째부터 증가해 3일째에 가장 높은 수치를 나타낸 후, 점점 감소해 ${E_2}$ 투여전과 유사한 수치를 나타내었다. 이들 결과로 E2에 노출 된 이후 조피볼락의 혈장 내 ALPP, Ca, GPT 및 HSI는 혈장 VTG와 유사한 증․감을 나타내는 것을 알 수 있었다. 따라서, ALPP, Ca, GPT 및 HSI는 VTG와 더불어 연안 생태계 내에서 외인성 ${E_2}$ 노출에 대한 생물학적 지표로서 사용 가능할 것으로 판단되어진다.

• 한국임상수의학회지
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• 제17권1호
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• pp.28-31
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• 2000

An animall health monitoring system in Gyeongnam area(near Chinju) was studied to analysis of biochemical risk factors in 617 herds. Clinical serum factors such as glutamate oxaloacetate transaminase(GOT), glutamate pyruvate transaminase(GPT), Ca, P, Mg, glucose, and cholesterol were measured with automatic biochemical analyzer(Ra-X7T Techmmicon, USA). In serum analysis, 613 cattle were within normal llimits(GOT: 9.5-85 IU-dl, GPT: 25-77IU/dl, total protein: 5.8-8.5g/dl, Ca: 4.2-12.4mg/dl, P: 4.6-9.7mg/dl, Mg: 1.5-3.0mg/dl, glucose: 48-120mg/dl, Cholesterol: 70-170mg/dl), the other cattle showed high glucose and high cholesterol level. It is proposed that clinical serum factors to be estimated may be valuable for developing of animal health monitoring system model.

• 대한물리의학회지
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• 제5권2호
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• pp.265-272
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• 2010

Purpose : This study was investigated the effect of electroacupuncture stimulation on the change of blood biochemical components in the rat spinal cord injury(SCI) damaged by the 6-hydroxydopamine. Methods : SCI model rats were damaged in L1-L2 injected with 6-hydroxydopamine. The thirty Sprague-Dawley adult male rats were randomly divided into normal group, control group and electroacupuncture group. Experimental groups were applied as electroacupuncture(Es-160, ITO, Japan) for 15minutes during the low frequency(2 Hz) stimulation to zusanli. The enzyme concentration levels analysis of the hematological changes were measured of Glutamate Oxaloacetate Transaminase(GOT), Glutamate Pyruvate Transaminase(GPT), Lactate dehydrogenase(LDH) and motor function recovery change was evaluated by the rota-rod test. Results : This study were as follow : The concentration of GOT, LDH in experimental group was lower than control group(p<.05). The experimental group showed increase of motor function recovery more in compared to control group(p<.05). Conclusion : The results of this study showed that electroacupuncture to zusanli point have an effect on functional recovery after the 6-hydroxydopamine induced SCI in rats.

• Journal of Radiation Protection and Research
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• 제17권1호
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• pp.11-19
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• 1992

카드뮴 독성의 반응을 파악하고 효과적인 치료방법을 제시하고자 백서에 $CdCl_2$ 용액을 경구투여하여 혈중 $K^+,\;Ca^{2+}$ 농도, glutamate oxaloacetate transaminase(GOT), glutamate pyruvate transaminase(GPT) 활성 및 c-AMP 농도를 경시적으로 측정하였다. 카드뮴 용액을 단독투여하면 혈중 $K^+$ 농도에 비해 $Ca^{2+}$ 농도의 감소가 유의하였으며, GOT와 GPT 및 c-AMP의 농도는 증가하였다. 카드뮴을 함유한 selenium 병용투여군과 metallothionein 병용투여군에서는 카드뮴 단독투여군에 비하여 혈중 $Ca^{2+}$ 농도에 유의한 변화가 없었다. 그러나 카드뮴의 체내 오염시 관찰할 수 있는 독성지표로 혈중 GOT 및 GPT활성과 c-AMP 농도의 상승이 selenium에 의하여 현저하게 감소하나 metallothionein에 의하여는 감소효과가 없었으므로, 카드뮴 투여로 증가한 이들 해독지표에 대한 보상효과는 selenium에서 현저함을 알 수 있었다.

• 한국수산과학회지
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• 제29권6호
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• pp.828-836
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• 1996

The histological changes of gill, liver, spleen and muscle, and respiration and blood variables and liver glycogen content were examined in black seabream, Acanthopagrus schlegeli. Fish were exposed to a high level of total ammonia nitrogen (10.4 mg/l) and recovered from exposure $(0.4{\pm}0.2mg/l)$ in a closed recirculating seawater system. In the process of exposure, mortality was $9\%$, and hyperplasia, necrosis or inflammation appeared in all tissues except for muscle. Oxygen consumption was decreased by $49\%$, and red blood cell (RBC) number, hematocrit and hemoglobin concentration were significantly decreased, while plasma glucose contents, activities of glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) increased. Liver glycogen content significantly increased from $6.6\%\;to\;10.4\%$. A large amount of hemosiderin was observed in the splenic tissue. During the recovery period, RBC number, hematocrit, hemoglobin concentration, GOT and GPT activities were returned to the normal status. Histological status of liver tissue was returned to the normal, but liver glycogen content was not recovered. During the recovery period, spleen melanin-macrophages temporarily increased, but subsequently decreased to the normal status.

• The Korean Journal of Physiology and Pharmacology
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• 제18권2호
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• pp.143-148
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• 2014

Arecoline is a major alkaloid of areca nuts which are widely chewed by southeast Asian and it manifests various toxic effects in different organs of human and animals. In this work, mature mice were treated by vitamins C plus E, arecoline, or both daily for four weeks. The results showed that arecoline significantly increased the levels of serum alkaline phosphatase (ALP), glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and significantly decreased the levels of reduced glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) in the liver tissues. Additionally, the body weight, testis weight, sperm counts, motility and normal sperms also were significantly decreased. The supplement of vitamins C and E can bring the activities of ALP and GPT to normal levels and partially restore the sperm counts compared to the arecoline-treated group but have no other positive effects. In conclusion, the vitamins C and E partially attenuated the arecoline-induced hepatotoxiciy but basically had on protective effects against the arecoline-induced testicular toxicity.

• 한국수산과학회지
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• 제47권6호
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• pp.860-868
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• 2014

The study was performed to investigate changes in hematological responses and antioxidative enzyme activities (superoxide dismutase, SOD; catalase, CAT) of Japanese eel Anguilla japonica following exposure to 0 (control), 2.33, 4.60, 6,64 and 8.78 mM nitrite-N in fresh water for 48 h. Hematological parameters such as plasma nitrite, electrolytes, cortisol, glucose, glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), hemoglobin (Hb), methemoglobin (metHb) and NADH-methemoglobin reductase (NMR) were measured. Plasma nitrite, cortisol, metHb and NMR increased directly with increasing ambient nitrite concentration, while Hb content showed a progressive decline. Levels of plasma potassium, GOT and GPT of the eel exposed to 6.64 mM ambient nitrite were significantly higher than the control fish. The activity of SOD and CAT in plasma, gill and liver of the eel following exposure to nitrite were augmented by increasing ambient nitrite. Levels of plasma nitrite, metHb, NMR, cortisol, glucose and antioxidative enzyme activities of the eel exposed to 2.33 mM ambient nitrite were significantly higher than the control fish. This study suggested that the eel acutely exposed to elevated ambient nitrite causes nitrite-induced stress responses, changes in antioxidative enzyme activities and hematological parameters.