• Journal of Microbiology and Biotechnology
• /
• 제21권8호
• /
• pp.846-853
• /
• 2011

Glycerol has become an attractive carbon source in the biotechnology industry owing to its low price and reduced state. However, glycerol is rarely used as a carbon source in Saccharomyces cerevisiae because of its low utilization rate. In this study, we used glycerol as a main carbon source in S. cerevisiae to produce 1,2-propanediol. Metabolically engineered S. cerevisiae strains with overexpression of glycerol dissimilation pathway genes, including glycerol kinase (GUT1), glycerol 3-phosphate dehydrogenase (GUT2), glycerol dehydrogenase (gdh), and a glycerol transporter gene (GUP1), showed increased glycerol utilization and growth rate. More significant improvement of glycerol utilization and growth rate was accomplished by introducing 1,2-propanediol pathway genes, mgs (methylglyoxal synthase) and gldA (glycerol dehydrogenase) from Escherichia coli. By engineering both glycerol dissimilation and 1,2-propanediol pathways, the glycerol utilization and growth rate were improved 141% and 77%, respectively, and a 2.19 g 1,2- propanediol/l titer was achieved in 1% (v/v) glycerolcontaining YEPD medium in engineered S. cerevisiae.

• 한국지열·수열에너지학회논문집
• /
• 제16권1호
• /
• pp.17-25
• /
• 2020

Ground-coupled heat pump (GCHP) systems have become an efficient alternative to conventional cooling and heating methods due to their higher energy using efficiency. Although some experimental and simulation works related to performance analysis of GCHP systems for commercial buildings have been done, relatively little has been reported on the performance evaluation of GCHP systems for school buildings. The purpose of this simulation study is to evaluate the performance of a hypothetical GCHP system for a school building in Seoul. We collected various data of building specifications and construction materials for the building and then modeled to calculate hourly building loads with SketchuUp and TRNSYS V17. In addition, we used GLD (Ground Loop Design) V2016, a GCHP system design and simulation software, to design the GCHP system for the building and to simulate temperature of circulating water in ground heat exchanger. The variation of entering source temperature (EST) into the system was calculated with different prediction time and then each result was compared. For 20 years of prediction time, EST for baseline design (Case A) based on the hourly simulation results were outranged from the design criteria.

• BMB Reports
• /
• 제44권3호
• /
• pp.147-157
• /
• 2011

The meiotic process from the primordial stage to zygote in female germ cells is mainly adjusted by post-transcriptional regulation of pre-existing maternal mRNA and post-translational modification of proteins. Several key proteins such as the cell cycle regulator, Cdk1/cyclin B, are post-translationally modified for precise control of meiotic progression. The second messenger (cAMP), kinases (PKA, Akt, MAPK, Aurora A, CaMK II, etc), phosphatases (Cdc25, Cdc14), and other proteins (G-protein coupled receptor, phosphodiesterase) are directly or indirectly involved in this process. Many proteins, such as CPEB, maskin, eIF4E, eIF4G, 4E-BP, and 4E-T, post-transcriptionally regulate mRNA via binding to the cap structure at the 5' end of mRNA or its 3' untranslated region (UTR) to generate a closed-loop structure. The 3' UTR of the transcript is also implicated in post-transcriptional regulation through an association with proteins such as CPEB, CPSF, GLD-2, PARN, and Dazl to modulate poly(A) tail length. RNA interfering is a new regulatory mechanism of the amount of mRNA in the mouse oocyte. This review summarizes information about post-transcriptional and post-translational regulation during mouse oocyte meiotic maturation.

• 설비공학논문집
• /
• 제29권5호
• /
• pp.249-258
• /
• 2017

This paper provides an economic analysis of a new geothermal heat pump system that reuses condenser waste heat from a Ground Source Heat Pump ($GSHP_{ch}$) to provide energy for a hot water Ground Source heat pump ($GSHP_{hw}$). After conducting feasibility tests using GLD and TRNSYS simulations, the proposed system was effectively installed and thoroughly tested. We observe that 1) the Coefficient of Performance (COP) of the $GSHP_{hw}$ and the $GSHP_{ch}$ during cooling mode improves by up to 62% and 7%, respectively; 2) the number of bore holes can be reduced by two; and 3) the hot water supply temperature of the $GSHP_{hw}$ increases by up to $60^{\circ}C$. We further conclude that 1) the reduction of two bore holes can save approximately ten million Won from the initial cost investment; and 2) the increased COP of the $GSHP_{hw}$ can save approximately one million Won in annual electricity costs.

• 대한화학회지
• /
• 제68권1호
• /
• pp.32-39
• /
• 2024

Glycerol dehydrogenase (GlyDH) plays a crucial role in the glycerol metabolism pathway by catalyzing the oxidation of glycerol to dihydroxyacetone (DHA). Previous studies of GlyDH have predominantly focused on unraveling the structural features of the active site and its binding interactions with ligand. However, the structural details of GlyDH in complex with both NAD⁺ and the substrate bound have remained elusive. In this study, we present the crystal structures of Klebsiella pneumoniae GlyDH (KpGlyDH) in the absence and presence of NAD⁺ at a resolution of 2.1 Å. Notably, both structures reveal the binding of the substrate, ethylene glycol, to the zinc ion. Interestingly, a significant change in the coordination number of the zinc ion is observed, with three in the absence of NAD⁺ and four in its presence. These findings shed light on the structural aspects of GlyDH and its interactions with NAD⁺ and the substrate.

• 대한설비공학회:학술대회논문집
• /
• 대한설비공학회 2009년도 하계학술발표대회 논문집
• /
• pp.579-585
• /
• 2009

Recently, Vertical-Closed Loop system using geothermal which is the most efficient among the building cooling and heating systems is coming into wide spread due to assistance of domestic policies. However, there is a limitation that a design of ground heat exchanger taking 60% of construction cost is done by GLD and GLHEPRO programs without specific guidelines and consideration on Entering Water Temperature(EWT). For getting an optimal EWT, we analyzed the costs for construction of ground heat exchanger and cooling and heating for 15 years. In the results, reduction of construction costs as the length of ground heat exchanger shortens was much greater than increase of the electrical power consumption as COP gets low. EWT that COP of heat pump can be 3.76 or above was below $31^{\circ}C$ in cooling and was over $5^{\circ}C$ in heating.

• Journal of Microbiology and Biotechnology
• /
• 제26권6호
• /
• pp.1077-1086
• /
• 2016

Glycerol dehydrogenases (GlyDHs) are essential for glycerol metabolism in vivo, catalyzing its reversible reduction to 1,3-dihydroxypropranone (DHA). The gldA gene encoding a putative GlyDH was cloned from Thermoanaerobacterium thermosaccharolyticum DSM 571 (TtGlyDH) and expressed in Escherichia coli. The presence of Mn²⁺ enhanced its enzymatic activity by 79.5%. Three highly conserved residues (Asp¹⁷¹, His²⁵⁴, and His²⁷¹) in TtGlyDH were associated with metal ion binding. Based on an investigation of glycerol oxidation and DHA reduction, TtGlyDH showed maximum activity towards glycerol at 60℃ and pH 8.0 and towards DHA at 60℃ and pH 6.0. DHA reduction was the dominant reaction, with a lower K_m(DHA) of 1.08 ± 0.13 mM and V_max of 0.0053 ± 0.0001 mM/s, compared with glycerol oxidation, with a K_m(glycerol) of 30.29 ± 3.42 mM and V_max of 0.042 ± 0.002 mM/s. TtGlyDH had an apparent activation energy of 312.94 kJ/mol. The recombinant TtGlyDH was thermostable, maintaining 65% of its activity after a 2-h incubation at 60℃. Molecular modeling and site-directed mutagenesis analyses demonstrated that TtGlyDH had an atypical dinucleotide binding motif (GGG motif) and a basic residue Arg⁴³, both related to dinucleotide binding.

• 설비공학논문집
• /
• 제26권9호
• /
• pp.409-415
• /
• 2014

Ground-source heat pump (GSHP) systems have become an efficient alternative to conventional cooling and heating methods due to their higher energy efficiency. These systems use the ground as a heat source and the heat sink for cooling mode operation. The purpose of this simulation study is to evaluate the performance of a hypothetical GSHP system in an office building and to assess the energy saving effect against the existing HVAC systems (boiler and turbo chiller). We collected monthly energy consumption data from an actual office building ($32,488m^2$) in Seoul, and created a model to calculate the hourly building loads with EnergyPlus. In addition, we used GLD (Ground Loop Design) V8.0, a GSHP system design and simulation software tool, to evaluate hourly and monthly performance of the GSHP system. The energy consumption for the GSHP system based on the hourly simulation results were estimated to be 582.6 MWh/year for cooling and 593.2 MWh/year for heating, while those for the existing HVAC systems were found to be 674.5 MWh/year and 2,496.4 MWh/year, respectively. The seasonal performance factor (SPF) of the GSHP system was also calculated to be in the range of 3.37~4.28.

• Journal of Microbiology and Biotechnology
• /
• 제30권2호
• /
• pp.271-278
• /
• 2020

Glycerol dehydrogenase (GlyDH) catalyzes the oxidation of glycerol to dihydroxyacetone (DHA), which is the first step in the glycerol metabolism pathway. GlyDH has attracted great interest for its potential industrial applications, since DHA is a precursor for the synthesis of many commercially valuable chemicals and various drugs. In this study, GlyDH from Klebsiella pneumoniae (KpGlyDH) was overexpressed in E. coli and purified to homogeneity for biochemical and molecular characterization. KpGlyDH exhibits an exclusive preference for NAD⁺ over NADP⁺. The enzymatic activity of KpGlyDH is maximal at pH 8.6 and pH 10.0. Of the three common polyol substrates, KpGlyDH showed the highest k_cat/K_m value for glycerol, which is three times higher than for racemic 2,3-butanediol and 32 times higher than for ethylene glycol. The k_cat value for glycerol oxidation is notably high at 87.1 ± 11.3 sec^-1. KpGlyDH was shown to exist in an equilibrium between two different oligomeric states, octamer and hexadecamer, by size-exclusion chromatography analysis. KpGlyDH is structurally thermostable, with a T_m of 83.4℃, in thermal denaturation experiment using circular dichroism spectroscopy. The biochemical and biophysical characteristics of KpGlyDH revealed in this study should provide the basis for future research on its glycerol metabolism and possible use in industrial applications.

• 대한의생명과학회지
• /
• 제13권4호
• /
• pp.287-291
• /
• 2007

The factors and mechanisms by infection of Oriental Tsutsugamushi caused disease are not well understood. The onset of tsutsugamushi disease is characterized by chilliness, fever, malaise, headache and generalized aching. Infection of tsutsugamushi is the cause of impairment of function of a major organ often complicate the picture and immediately change the prognosis for the worse. Tsutsugamushi disease is reported that this disease is characterized by the histopathogenesis of liver, kidney, heart, and lung, but the variation of biochemical components in serum of tsutsugamushi disease patient are not clear. We analyzed total protein (TP), albumin (AL), aspartic aminotranferase (AST), alanine aminotransferase (ALT), alkaline phosphotase (ALP), urea nitrogen (UN), creatinine (CRE), glucose (GLD), cholesterol (CHOL) and total bilirubin (TB) in sera of patients with tsutsugamushi disease. In comparison with reference, total protein and albumin were abnormally decreased in 19.6% and 39.2% of patients, respectively. AST, ALT, ALP, creatinine, UN, glucose, cholesterol and total bilirubin were abnormally increased in 94.1 %, 72.5%, 25.5%, 15.7%, 9.8%, 62.7%, 25.5% and 6.0% of patients, respectively. The patients showed abnormal relative rate of protein electrophoretic fractions to total protein in serum compared to them of reference were 43.1% (albumin), 12.9% ($\alpha_1$-globulin), 58.8% ($\alpha_2$-globulin), 60.8% ($\beta$-globulin) and 70.6% ($\gamma$-globulin), respectively. These data suggest that infection of Oriental Tsutsugamushi causes impairment of function of a major organ and abnormal serum protein electrophoresis fractions to tsutsugamushi patients.