• Food Science and Biotechnology
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• 제18권2호
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• pp.565-569
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• 2009

This study was conducted to evaluate the effects of different preparation methods on the recovery and quantification of ginsenosides in raw Korean ginseng (Panax ginseng C.A. Meyer). Eight major ginsenosides ($Rb_1$, $Rb_2$, $Rb_3$, Rc, Rd, Re, Rf, and $Rg_1$) were analyzed by high performance liquid chromatography (HPLC), after which the recovery and repeatability of the extraction of those ginsenosides using 3 different preparation methods were compared [A. direct extraction (DE) method, hot MeOH extraction/evaporation/direct dissolution; B. solid phase extraction (SPE) method, hot MeOH extraction/evaporation/dissolution/$C_{18}$ cartridge adsorption/MeOH elution; C. liquid-liquid extraction (LLE) method, hot MeOH extraction/evaporation/dissolution/n-BuOH fractionation]. Use of the DE method resulted in a significantly higher recovery of total ginsenosides than other methods and a relatively clear peak resolution. Use of the SPE and LLE methods resulted in clearer peak resolution, but lower ginsenoside recovery than the DE method. The LLE method showed the lowest ginsenoside recovery and repeatability among the 3 methods. Given that the DE method employed only extraction, evaporation, and a dissolution step (avoiding complicate and time consuming purification), this technique may be an effective method for the preparation and quantification of ginsenosides from raw Korean ginseng.

• Journal of Ginseng Research
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• 제20권1호
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• pp.15-22
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• 1996

In order to investigate the Immunomodulatory effects of ginseng, we have studied the effects of ginseng saponin on the proliferation and cytosine gene expression of human pheripheral blood mononuclear cell (PBMC). In the PBMC proliferation assay, total saponin exhibited proliferation inhibition on the PBMC or phytohemagglutinin(PHA)-stimulated PBMC in a dose-dependent fashion. Immunomodulatory effects of ginseng were further investigated using the cytokine gene expression as the indicators. In the reverse transcription-polymerase chain reaction (RT-PCR) test, interleukin (IL)-1, IL-2, IL-3, IL-4, IL-6, IL-13, granulocyte macrophage-colony stimulating factor, tumor necrosis factor (TNF), migration inhibitory factor and transforming growth factor genes were expressed in the PHA-stimulated PBMC 48 hrs after cell culture. Among expressed cytokines, total saponin could increase the expression of IL-1 and TNF of PBMC without stimulation of PHA. All of ginsenosides, $Rb_1$, $Rb_2$, $Rg_1$, Rc, Re, incresed TNF gene expression. Especially, Rb2 (20 g/ml) showed most prominent effect on TNF gene expression and it also slightly increased IL-1 gene expression of PBMC.

• 한국식품과학회지
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• 제10권2호
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• pp.181-188
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• 1978

1년생(年生) 수삼근(水蔘根)과 엽병(葉柄)(petiole)으로 조직배양(組織培養)한 callus 양자간(兩者間)의 유효성분(有效成分)인 saponins 및 sapogenins에 관하여 비교(比較) 검토(檢討)한 결과(結果)는 다음과 같다. (1) 인삼(人蔘)과 callus는 Liebermann-Burchard 반응(反應)에서 양성(陽性)으로 확인(確認)되었다. (2) Ginsenoside $Rx[x=o,a,b_1,b_2,c,e,d,g(f)]$의 Rf치(値)는 거의 일치(一致)하였으며 상대함량비(相對含量比)는 인삼(人蔘)에서 Rb,c : Rg(f)=21.1 : 20.5 (1.03 : 1), Rb,c,d : Re,g(f)=22.8 : 26.9(1 : 1.17)이었으며, callus에서 Rb,c : Rg(f)= 18.2 8.4(2.16 : 1) Rb,c,d : Re,g(f)=19.28 : 31.6(1 : 1.63)이었다. (3) 인삼(人蔘)과 callus의 sapogenins에 있어서 diol; triol, oleanolic acid, ${\beta}-sitosterol$의 Rf치(値)는 거의 일치(一致)하였으며 상대함량비(相對含量比)는 인삼(人蔘)에서 diol : triol = 13.8 : 7.4(1.86 : 1)이었고, callus에서 diol : triol = 19.2 : 7.2(2.66 : 1)이었다.

• 한국미생물·생명공학회지
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• 제13권4호
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• pp.367-370
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• 1985

인삼농축액이 미생물에 의하여 품질이 저하되는 것을 방지하기 위한 기초자료를 얻고자 부패된 홍삼농축액으로부터 효모를 분리 동정하였고 홍삼농축액의 일반성분 및 사포닌의 조성을 조사하였다. 분리, 선발된 홍삼농축액의 부패성 효모는 형태학적, 배양학적 및 생리학적 특성을 검토한 결과 candida parapsilosis로 동정되었다. 홍삼농축액의 일반성분은 회분 3.85, 조지방 1.48, 조단백 15.3. 환원당 12.4, 전당 45.6% 및 기타성분으로 구성되어 있었다. 조사포닌 함량은 18.24%이었으며 각 ginsenoside의 구성은 Rb$_1$, Rg$_1$, Rc, Rb$_2$, Rf, Ra, Ro순이었으며 panaxadiol계 및 panaxatriol 계 saponin의 구성비는 1.56이었다.

• Journal of Ginseng Research
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• 제15권3호
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• pp.257-262
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• 1991

1) 인삼 사포닌을 5% 황산으로 가수분해하여 TMS화 한 후 GC로 분석한 결과 인삼중의 모든 사포닌을 diol계 사포닌과 triol계 사포닌으로 나누어 분석할 수 있었다. 2) 시료중의 전체 diol계(PD)와 triol계(PT) 사포닌을 ginsenoside Rb$_1$과 Rg$_1$의 양으로 각각 환산하여 표시하고 미삼에는 백삼에 비해 diol계 사포닌의 함량이 많은 것을 이용하여 PD/PT글 구하고 여기에서 구해진 비를 이용하여 시료중의 백삼 및 미삼의 이론적 함량을 구할 수 있었다. 3) 이 방법의 검출 한계는 백삼의 양으로 0.14$\mu$g이었다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.138.1-138.1
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• 2003

Inflammatory responses from activated microglia are one of major causes of Alzheimer's disease (AD). Particularly, proinflammatory cytokines (PC), such as IL-l$\beta$ and TNF-$\alpha$, and nitric oxide (NO) are correlated with AD by inducing the chronic inflammation in the brain. In the present study, we found that microglia are activated by lipopolisaccharide (LPS) and Abeta42 (A$\beta$42), and those activated microglia produced such repertoires up to 72h with a turning point at 24h. However, no dose dependecy was found during the chasing time courses (6h to 72h). (omitted)

• Journal of Ginseng Research
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• 제27권4호
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• pp.183-187
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• 2003

본 연구는 Panax속의 유용형질을 탐색하고, 고려인삼과 미국삼, 그리고 종간 잡종인 F$_1$ 식물체의 생육, 형태적 특성, 수량, 적변율 및 ginsenoside함량 등을 조사하였던 바, 그 결과를 요약하면 다음과 같다. 1. 고려인삼은 적변 발생율이 연생이 높아질수록 증가하였으나, 미국삼은 적변이 발생되지 않았으며 F$_1$잡종식물체에서도 적변이 발생되지 않았다. 2. 고려인삼과 미국삼의 잡종인 F$_1$잡종식물체 인삼의 개화기는 미국삼보다 빠르고, 자경종보다는 늦었다. 3. 고려인삼과 미국삼을 교배한 F$_1$ 묘삼의 근형질 특성은 미국삼과 고려인삼에 비하여 근장과 근중이 컸다. 4. 잡종 F$_1$의 ginsenosides 함량은 대부분이 고려인삼과 미국삼의 중간 정도였으나, 양친보다 Re는 많았고 Rg$_1$, Rg$_2$ 는 적게 정량 분석되었으며, Rf가 고려인삼에서는 검출되었으나 미국삼 및 F$_1$잡종식물체에서는 검출되지 않았다.

• Journal of Ginseng Research
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• 제44권4호
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• pp.563-569
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• 2020

Background: White ginseng consists of the roots and rhizomes of the Panax species, and red ginseng is made by steaming and drying white ginseng. While red ginseng has both polar and nonpolar ginsenosides, previous studies showed white ginseng to have only polar ginsenosides. Because nonpolar ginsenosides are formed through the manufacture of red ginseng from white ginseng, researchers have generally thought that nonpolar ginsenosides do not exist in white ginseng. Methods: We developed a simultaneous quantitative method for six nonpolar ginsenosides in white ginseng using reverse-phase high-performance liquid chromatography coupled with integrated pulsed amperometric detection. The nonpolar ginsenosides of white ginseng were extracted for 4 h under reflux with 50% methanol. Results: Using the gradient elution system, all target components were completely separated within 50 min. Nonpolar ginsenosides were determined in the rhizome head (RH), main root (MR), lateral root, and hairy root (HR) of 6-year-old white ginseng samples obtained from several regions (Geumsan, Punggi, and Kanghwa). The total content in the HR of white ginseng was 37.8-56.8% of that in the HR of red ginseng. The total content in the MR of white ginseng was 5.9-24.3% of that in the MR of red ginseng. In addition, the total content in the RH of white ginseng was 28.5-35.8% of that in the HR of red ginseng Conclusion: It was confirmed that nonpolar ginsenosides known to be specific components of red ginseng were present at substantial concentrations in the HR or RH of white ginseng.

• Journal of Ginseng Research
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• 제45권6호
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• pp.631-641
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• 2021

Background: Main bioactive constituents and pharmacological functions of ripened red ginseng berry (Panax ginseng Meyer) have been frequently reported. Yet, the research gap targeting the beneficial activities of transformed green ginseng berries has not reported elsewhere. Methods: Ginsenosides of new green berry cultivar K-1 (GK-1) were identified by HPLC-QTOF/MS. Ginsenosides bioconversion in GK-1 by bgp1 enzyme was confirmed with HPLC and TLC. Then, mechanisms of GK-1 and β-glucosidase (bgp1) biotransformed GK-1 (BGK-1) were determined by Quantitative Reverse Transcription-Polymerase Chain Reaction and Western blot. Results: GK-1 possesses highest ginsenosides especially ginsenoside-Re amongst seven ginseng cultivars including (Chunpoong, Huangsuk, Kumpoong, K-1, Honkaejong, Gopoong, and Yunpoong). Ginseng root's biomass is not affected with the harvest of GK-1 at 3 weeks after flowering period. Then, Re is bioconverted into a promising pharmaceutical effect of Rg2 via bgp1. According to the results of cell assays, BGK-1 shows decrease of tyrosinase and melanin content in α-melanocyte-stimulating hormone challenged-murine melanoma B16 cells. BGK-1 which is comparatively more effective than GK-1 extract shows significant suppression of the nuclear factor (NF)-κB activation and inflammatory target genes, in LPS-stimulated RAW 264.7 cells. Conclusion: These results reported effective whitening and anti-inflammatory of BGK-1 as compared to GK-1.

• 한국식품과학회지
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• 제37권1호
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• pp.67-72
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• 2005

수삼 및 수삼을 기질로 한 배양물로부터 조사포닌을 분리하여 TLC를 확인한 결과 목질진흙버섯 및 영지버섯 균사체 배양물 조사포닌에서 $Rg_{2}$와 $Rh_{1}$ 위치에서 spot을 볼 수 있었으며, 노루궁뎅이버섯 균사체 배양물에서는 $Rg_{3}$와 $Rh_{1}$과 같은 위치에서 spot을 볼 수 있었다. 배양물의 총페놀성 화합물 함량은 수삼, 목질진흙버섯, 영지버섯 및 노루궁뎅이버섯 균사체 배양물 조사포닌에서 1.04, 1.11, 1.45, 1.29%로 나타나 수삼보다는 수삼 배양물에서 높았으며, 특히 영지버섯 균사체 배양물에서 가장 높은 폐놀성 화합물 함량을 나타내었다. 수삼 배양물 조사포닌의 전자공여능의 경우 목질진흙버섯 균사체 배양물 1mg/mL 및 10mg/mL에서 각각 92.8, 94.9%로 가장 높았다. 지질과산화 억제효과는 영지버섯 균사체 배양물 조사포닌이 29.5%로 목질진흙버섯 균사체나 노루궁뎅이버섯 균사체 배양물 보다 높았다. Tyrosinase 저해활성은 영지버섯 균사체 배양물 조사포닌이 65.5%로 가장 높았다.