• Journal of Ginseng Research
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• 제35권2호
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• pp.235-242
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• 2011

To obtain microorganisms for the microbial conversion of ginsenosides in red ginseng extract (RGE), mushroom mycelia were used for the fermentation of RGE. After fermentation, total sugar contents and polyohenol contents of the RGEs fermented with various mushrooms were not a significant increase between RGE and the ferments. But uronic acid content was relatively higher in the fermented RGEs cultured with Lentus edodes (2155.6 ${\mu}g/mL$), Phelllinus linteus (1690.9 ${\mu}g/mL$) and Inonotus obliquus 26137 and 26147 (1549.5 and 1670.7 ${\mu}g/mL$) compared to the RGE (1307.1 ${\mu}g/mL$). The RGEs fermented by Ph. linteus, Cordyceps militaris, and Grifola frondosa showed particularly high levels of total ginsenosides (20018.1, 17501.6, and 16267.0 ${\mu}g/mL$, respectively). The ferments with C. militaris (6974.2 ${\mu}g/mL$), Ph. linteus (9109.2 ${\mu}g/mL$), and G. frondosa (7023.0 ${\mu}g/mL$) also showed high levels of metabolites (sum of compound K, $Rh_1$, $Rg_5$, $Rk_1$, $Rg_3$, and $Rg_2$) compared to RGE (3615.9 ${\mu}g/mL$). Among four different RGE concentrations examined, a 20 brix concentration of RGE was favorable for the fermentation of Ph. linteus. Maximum biotransformation of ginsneoside metabolites (9395.5 ${\mu}g/mL$) was obtained after 5 days fermentation with Ph. linteus. Maximum mycelial growth of 2.6 mg/mL was achieved at 9 days, in which growth was not significantly different during 5 to 9 days fermentation. During fermentation of RGE by Ph. linteus in a 7 L fermenter, $Rg_3$, $Rg_5$, and $Rk_1$ contents showed maximum concentrations after 5 days similar to flask fermentation. These results confirm that fermentation with Ph. linteus is very useful for preparing minor ginsenoside metabolites while being safe for foods.

• Journal of Ginseng Research
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• 제29권2호
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• pp.107-112
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• 2005

본 실험은 수분함량 $15\%$로 건조한 수삼을 사출구온도($100^{\circ}C$와 $120^{\circ}C$), 스크루 회전속도(200과 300 rpm)에서 압출성형처리하여 조사포닌,진세노사이드, 말톨, 환원당, 색도와 같은 홍삼성분의 변화를 분석하였다. 압출성형공정을 통해 조사포닌 함량이 증가하는 것으로 보아사포닌의 압출성형공정동안 생성되는 것을 알 수가 있었다. 홍삼에 존재하는 진세노사이드 $Rg_l$과 $Rg_2$는 사출구온도를 $100^{\circ}C$에서 $120^{\circ}C$로 증가함에 따라 0.227 mg/g에서 0.2835 mg/g$(Rg_l)$, 0.1164 mg/g에서 0.2230 mg/g$(Rg_2)$증가하였고, 스크루 회전속도를 300rpm에서 200rpm으로 감소시켰을 때 진세노사이드 $Rg_1$ 과 $Rg_2$는 증가하는 경향을 나타내었다. 또한 수삼이나 백삼에는 함유되지 않고 홍삼에만 함유된 특이성분으로 알려진 말톨을 압출성형 수삼에서 확인할 수가 있었다. 사출구의 온도가 $100^{\circ}C$에서 $120^{\circ}C$로 증가함에 따라 총당의 함량은 크게 변화가 없었지만 환원당은 온도가 증가할수록 감소하는 경향을 보였다. 결론적으로 사출구온도와 스크루 회전속도 이외의 압출성형 공정변수의 조절을 통해 압출성형공정을 홍삼제조 공정에 적용할 수 있는 가능성을 알 수가 있었다.

• Journal of Ginseng Research
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• 제40권2호
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• pp.105-112
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• 2016

Background: Minor saponins or human intestinal bacterial metabolites, such as ginsenosides Rg3, F2, Rh2, and compound K, are more pharmacologically active than major saponins, such as ginsenosides Rb1, Rb2, and Rc. In this work, enzymatic hydrolysis of ginsenoside Rb1 was studied using enzyme preparations from cultured mycelia of mushrooms. Methods: Mycelia of Armillaria mellea, Ganoderma lucidum, Phellinus linteus, Elfvingia applanata, and Pleurotus ostreatus were cultivated in liquid media at $25^{\circ}C$ for 2 wk. Enzyme preparations from cultured mycelia of five mushrooms were obtained by mycelia separation from cultured broth, enzyme extraction, ammonium sulfate (30-80%) precipitation, dialysis, and freeze drying, respectively. The enzyme preparations were used for enzymatic hydrolysis of ginsenoside Rb1. Results: Among the mushrooms used in this study, the enzyme preparation from cultured mycelia of A. mellea (AMMEP) was found to convert ginsenoside Rb1 into compound K with a high yield, while those from G. lucidum, P. linteus, E. applanata, and P. ostreatus produced remarkable amounts of ginsenoside Rd from ginsenoside Rb1. The enzymatic hydrolysis pathway of ginsenoside Rb1 by AMMEP was $Rb1{\rightarrow}Rd{\rightarrow}F2{\rightarrow}$ compound K. The optimum reaction conditions for compound K formation from ginsenoside Rb1 were as follows: reaction time 72-96 h, pH 4.0-4.5, and temperature $45-55^{\circ}C$. Conclusion: AMMEP can be used to produce the human intestinal bacterial metabolite, compound K, from ginsenoside Rb1 with a high yield and without food safety issues.

• 한국식품영양과학회지
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• 제41권8호
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• pp.1151-1157
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• 2012

인삼의 소비촉진과 국민의 건강 증진을 목적으로 인삼을 수삼과 홍삼액의 형태로 첨가하여 인삼 밥을 취반한 후 기호도, 물성 및 사포닌과 유리아미노산의 함량을 분석하였다. 전반적으로 기호도가 가장 좋은 인삼 밥은 마쇄기로 거칠게 간 형태(GRG)의 수삼을 원료 쌀의 10%를 첨가하여 취반한 인삼 밥이었고, 조직감과 밥맛에 대한 기호도는 홍삼액(RGE)을 50% 첨가하였을 때이었다. 인삼 밥의 물성은 쌀알 크기의 1~2배 정도로 잘게 다진 형태의(MRG)의 수삼을 첨가할 경우 10% 이상 첨가 시부터는 hardness와 adhesiveness가 감소하였으며, 홍삼액 형태(RGE)로 밥물 대신 첨가하여 취반하였을 경우는 첨가량이 많아질수록 hardness는 증가하였으며 adhesiveness는 감소하였다. 믹서로 갈은 슬러지 형태의 수삼(GRG)과 잘게 다진 형태(MRG)의 수삼을 첨가하여 인삼 밥을 취반할 경우 취반과정 중 사포닌구조의 변화가 일어나 수삼에서 검출되어진 Re를 포함한 8종의 진세노사이드성분의 함량이 감소되고 홍삼특유의 사포닌인 Rg3, Rh2 및 Rh1 등의 사포닌이 생성되었다. 총 유리아미노산 함량은 수삼 및 홍삼액 모두 첨가량이 증가할수록 인삼밥의 총 유리아미노산 함량이 증가되었다.

• Journal of Ginseng Research
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• 제41권4호
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• pp.435-443
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• 2017

Panax ginseng is one of the most universally used herbal medicines in Asian and Western countries. Most of the biological activities of ginseng are derived from its main constituents, ginsenosides. Interestingly, a number of studies have reported that ginsenosides and their metabolites/derivatives-including ginsenoside (G)-Rb1, compound K, G-Rb2, G-Rd, G-Re, G-Rg1, G-Rg3, G-Rg5, G-Rh1, G-Rh2, and G-Rp1-exert anti-inflammatory activities in inflammatory responses by suppressing the production of proinflammatory cytokines and regulating the activities of inflammatory signaling pathways, such as nuclear factor-${\kappa}B$ and activator protein-1. This review discusses recent studies regarding molecular mechanisms by which ginsenosides play critical roles in inflammatory responses and diseases, and provides evidence showing their potential to prevent and treat inflammatory diseases.

• Journal of Ginseng Research
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• 제30권2호
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• pp.70-77
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• 2006

Ginseng has an anti-cancer effect in several cancer models. As a mechanism study of ginsenoside-induced growth inhibition in cancer cells, we measured change of membrane potential in prostate cancer and glioma cells by ginsenosides, active constituents of ginseng. Membrane potential was estimated by measuring fluorescence change of DiBAC-Ioaded cells. Among 11 ginsenosides tested, ginsenosides $Rb_2$, $Rg_3$, and $Rh_2$ increased significantly and robustly the membrane potential in a concentration-dependent manner in prostate cancer and glioma cells. Ginsenosides Rc, Ro, and $Rb_1$ slightly increased membrane potential. The ginsenoside-induced membrane potential increase was not affected by treatment with pertussis toxin or U73122. The ginsenoside-induced membrane potential increase was not diminished in $Na^+$-free or $HCO_3^-$-free media. Furthermore, the ginsenoside-induced increase of membrane potential was not changed by EIPA (5-(N-ethyl-N-isopropyl)-amiloride), SITS (4-acetoamido-4'-isothiocyanostilbene-2,2'-disulfonic acid), and omeprazole. In summary, ginsenosides $Rb_2$, $Rg_3$, and $Rh_2$ increased membrane potential in prostate cancer and glioma cells in a GPCR-independent and $Na^+$ independent manner.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1984년도 학술대회지
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• pp.169-183
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• 1984

인삼의 주된 사포닌으로서 Rg,과 Rb,을 흰쥐에 투여하였을 경우에 이들 물질이 흰쥐의 장기에 흡수 또는 분포되는 상태와 배설에 대하여 연구하였다. 진세노사이드 $Rg_{1}$은 경구 투여량의 약 $1.9{\%}$가 소화관의 상부에서 흡수되었으며, 투여한지 30분 후에 최고 혈중 농도에 이르렀고 조직에서는 1.5시간 걸렸다. 그러나 뇌에서는 확인되지 않았으며 뇨와 당즙에는 2 : 5의 비로 배설되었다. $Rb_{1}$을 100mg/kg 경구투여한 결과, 소화관에서는 거의 흡수가 되지 않았으며, 한편 정맥주사(5mg/kg)의 경우는 혈중 $Rb_{1}$의 농도가 지수적으로 감소하였으며, B-phase의 반감기는 14.5시간이었다. 정맥주사후 혈청과 조직에 장시간 잔존은 활성을 나탄내는 혈청단백과의 결합과 관련이 있는 것으로 사료되며 시간에 따라 뇨로 배설되나 담즙에서는 확인되지 않았다. $Rg_{1}$과 $Rb_{1}$을 경구투여한 후 TLC와 $^{13}C$-NMR을 이용하여 위와 대장에서의 분해 상태를 연구한 결과 위에서 $Rg_{1}$의 일부가 분해, 6종류의 분해 산물이 r-everse phase TLC상에서 관찰되었고 이들 분해 산물은 약산성 (0.1N HCl, $37^{\circ}C$) 조건하에서 $Rg_{1}$의 가수분해산물과 동일하였다. 한편, $Rb_{1}$ 경구투여후 위장에서 얻은 시료중에서 미확인 분해산물이 관찰되었으며, 이 분해산물은 약산성 조건하에서 $Rb_{1}$의 가수분해산물과는 상이하다는 사실을 확인하였다. 대장에서, $Rg_{1}$은 미생물 tetracycline-susceptible bacteria와 tetracycline-resist bacteria에 의해 $Rb_{1}$과 $F_{1}$으로 분해되었으며, $Rb_{1}$은 장내의 효소와 tetracycline-resistantant bacteria에 의해 Rd와 2 종류의 미확인 물질로 분해되었다.

• Journal of Ginseng Research
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• 제30권3호
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• pp.95-99
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• 2006

When the inhibitory effect of ginsenoside (G) Re isolated from ginseng and its metabolites G-Rg1, G-F1, G-Rh1 and protopanaxatriol in mouse ear skin psoriasis stimulated by oxazolone was investigated, G-Re and its metabolites suppressed mouse ear swelling stimulated by oxazolone. Among these agents tested, G-Rh1 most potently suppressed ear swelling as well as mRNA expression of COX-2 and proinflammatory cytokines $IL-1{\beta},\;TNF-{\alpha}$ and $interferon-{\gamma}$. These findings suggest that G-Rh1 may improve chronic dermatitis and psoriasis.

• 한국식생활문화학회지
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• 제16권5호
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• pp.478-482
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• 2001

This study was conducted to investigate the difference of general feature and ginsenoside content of 6 years old ginseng root among different grade of roots. Total weight of a 1st grade-6 years old ginseng root was 115.1g and weight, length, diameter and specific gravity of main root were 64.68g, 8.39cm, 3.31cm and 0.96, respectively. Main root of 1st grade ginseng root was larger in size and specific gravity and more heavy than that of End or 3rd grade of the roots. Though crude saponin contents were not so different among the different grade of roots, but ginsenoside Rb1, Rg1 and Re content were higher in 1st grade of root than that of 2nd or 3rd grade of root. Those ginsenosides were located mainly in periderm and cortex.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2007년도 추계학술발표회
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• pp.337.1-337.1
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• 2007