• 식물조직배양학회지
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• 제27권4호
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• pp.309-315
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• 2000

Panax ginseng is an important medicinal plant that has been used worldwide for geriatric, tonic, stomachic, and aphrodisiac treatments. Ginsenosides contained in the ginseng root are the main substances having active functions for human body. The price of ginseng is very expensive due to a complex process of cultivation, and the yield of ginseng is limited, which cannot meet the demand of the increasing market. Researchers have applied plant biotechnology to solve the problems but there are still things to be determined towards ginsenoside production by large-scale adventitious root culture. In this experiment, 5 to 20 liter bioreactors were employed to determine optimal conditions for adventitious root culture and ginsenoside production of Panax gineng. Callus was induced from the ginseng root on MS agar medium containing 1.0 mg. $L^{-1}$ 2,4-D and 0.1 mg. $L^{-1}$ kinetin. Then the callus was cultured on MS agar medium supplemented with 2.0 mg. $L^{-1}$ IBA, 0.1 mg. $L^{-1}$ kinetin, and 30 g. $L^{-1}$ to induce adventitious roots. The maximum root growth and ginsenoside production were obtained in 1/2 MS medium. 2.0 mg. $L^{-1}$ naphthalene acetic acid resulted in greater root growth than 2.0 mg $L^{-1}$ indole-3-butyric acid. Ginsenoside content increased with 2.0 mg. $L^{-1}$ benzyl adenin or kinetin. High concentrations of benzyl adenin (above 3.0 mg. $L^{-1}$ ) decreased the adventitious root growth and ginsenoside productivity. N $H_{4}$$^{+}$ inhibited the ginsenoside accumulation, while high concentrations of $K^{+}$, $Mg_{2}$$^{+}$, and $Ca_{2}$$^{+}$ increased it. N $H_{4}$$^{+}$ at 0.5 and 1.0 times of the normal amount in 3/4 SH medium resulted in the greatest biomass increase, but the highest ginsenoside productivity was obtained when N $O_{3}$$^{-}$ was used as the sole nitrogen source in the medium. Most microelements at high concentrations in the medium inhibited the root growth, but high concentrations of MnS $O_4$enhanced the root growth. Root dry weight increased with increasing sucrose concentrations up to 50 g. $L^{-1}$ , but decreased from 70 g $L^{-1}$ Ginsenoside productivity was maximized at the range of 20 to 30 g. $L^{-1}$ sucrose. In the experiment on bioreactor types, cone and balloon types were determined to be favorable for both adventitious root growth and ginsenoside production. Jasmonic acid was effective for increasing ginsenoside contents and Rb group ginsenosides mainly increased. These results could be employed in commercial scale bioreactor cultures of Panax ginseng.x ginseng.

• Journal of Ginseng Research
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• 제11권2호
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• pp.118-122
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• 1987

인삼의 재배과정중에 부산물로 산출되는 지상부위의 활용방안을 모색하기 위하여 엽과 경의 반성분, 용매별 엑기스 및 사포닌함량을 근의 함량과 대비 검토하여 다음과 같은 결과를 얻었다. 1. 엽은 총당의 함량이 21.5%로 근보다 낮았으나 조직유질, 조지방질 및 회분은 각각 9.41%, 3.43% 및 6.83%로 높았다. 경은 조직유질이 39.2%로 현저하게 많았고 총당과 조단백질은 22.7% 및 8.54%로 근보다 낮았다. 2. 용매별 추출물의 수율은 추출용매의 극성이 클수록 높았으며 근과 경에 비하여 엽은 methanol 및 ehanol 추출물의 수율이 35.9% 및 27.3%로 현저하게 많았고, acetone 및 ethyl acetate추출물도 5.64% 및 3.52%로 높았으며 그 외의 비극성 용매의 추출물의 수율도 대체로 높았다. 3. 총 조saponin의 수율은 근과 경은 4.78% 및 2.22%였으나 엽은 19.58%로 현저하게 높았다. HPLC에 의한 분석결과 엽에는 ginsenoside-Rg1(3.32%), -Re(3.24%), -Rd(2.32 %), -Rc(0.65%), -Rb2(0.92%), -Rbl(0.29%), and -Rf(0.11%)가 함유되었고, 경에는 ginsenoside-Rgl(0.28%), -Re(0.3%), -Rd(0.05%), -Rf(0.11%)외에 미량의 -Rbl, -Rb2, -Rc가 검출되었다. 특히 엽은 총 saponin과 ginsenoside-Rg1, -Re 및 -Rd외에도 -Rc와 -Rb2의 함량이 높아서 이들 성분의 분리용 시료로 적합함을 알 수 있었다.

• Applied Biological Chemistry
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• 제34권4호
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• pp.339-343
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• 1991

저장온도(실온, $35^{\circ}C$, $50^{\circ}C$)와 저장기간($1{\sim}10$개월)별로 보관한 인삼드링크제품의 $CO_2$ 압력, pH, 침전물 생성, 당도, 유리당, 색도, 조사포닌 및 ginsenoside 함량변화를 조사한 결과 $CO_2$ 압력은 실온에서 9개월후, $35^{\circ}C$에서 6개월후 및 $50^{\circ}C$에서 3개월후부터 각각 감소되었다. pH와 당도는 저장온도와 저장기간에 관계없이 각각 $3.17{\sim}3.21$과 $14.3{\sim}14.6$을 나타내었다. 색도, glucose와 fructose 및 panaxatriol계 사포닌은 저장온도가 높고 저장기간이 길수록 증가하였으나 sucrose와 panaxadiol계 사포닌은 감소하였다.

• Natural Product Sciences
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• 제20권2호
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• pp.119-125
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• 2014

According to the results of my study on the chromatographic analysis of fresh ginseng (Panax ginseng C. A. Meyer) roots, most of the contents of protopanxadiol ginsenosides $Rb_1$, Rc, $Rb_2$, and Rd are derived from the corresponding malonyl ginsenosides in fresh ginseng by a heat process. Also, I confirmed that acetyl ginsenosides are naturally occurring constituents in fresh ginseng, not decarboxylates from malonyl ginsenosides. Seven neutral ginsenosides $Rg_1$, Re, Rf, Rc, $Rb_1$, $Rb_2$, and Rd were transformed to specific conversions in red ginseng preparation conditions. The conversion paths progress by three rules concluded from my study. These conversion rules are I: the ether bond is stable at positions 3 and 6 in the dammarane skeleton, II: the ether bond between sugars is stable in glycosides, and III: the ether bond to glycosides is unstable at position 20 in the dammarane skeleton.

• 대한한의학회지
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• 제17권1호
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• pp.478-493
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• 1996

The components of Red-ginseng radix extract solution for herb- acupuncture were analyzed by HPLC. According to the Medical Product Safety Administration Guidelines for safety assessment, mice and rats were used for acute toxicity test. The results were summarized as follows; 1. In the Saponin contents(%) of Red-ginseng radix extract, Ginsenoside $Rb_1$ Saponin was 0.27% in raw material, 1.67% in extract powder and Ginsenoside Rc Saponin was 0.16% in raw material, 1.12% in extract powder and Ginsenoside Rd Saponin was 0.08% in raw material, 0.54% in extract powder. 2. There were no abnormal findings in acute toxicity test treated with Red-ginseng radix extract solution for herb-acupuncture and $LD_{50}$ could not be measured.

• Journal of Ginseng Research
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• 제33권4호
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• pp.349-354
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• 2009

흑삼의 속성제조와 ginsenoside $Rg_3$ 함량을 극대화하고자 흑삼 제조시 포도주스에 24시간 침지한 후 $120^{\circ}C$에서 30분간 3회 반복 증숙하여 흑삼을 제조한 후 HPLC 방법을 이용하여 ginsenosides를 분석하였다. 포도주스에 침지하여 제조한 흑삼의 ginsenoside $Rg_3$ 함량은 10.91 mg/g으로 구증구포 방법으로 제조한 흑삼보다 약 2배 가량 함량이 증가되었다. 총 사포닌 함량은 14.97 mg/g으로 전통적인 구증구포 방식으로 제조한 흑삼 (12.79 mg)보다 그 함량이 높았다. 흑삼의 단회투여 (200 mg/kg, p.o.)에 의한 뇌조직 AChE 활성은 투여 24시간 후에 유의적으로 억제되는 효과를 보여주었다. 따라서 본 연구에 적용한 새로운 제조방법은 ginsenoside $Rg_3$를 강화하는 흑삼의 속성제조에 효과적인 방법으로 판단된다. 또한, AChE 활성억제를 통해 흑삼이 뇌기능 개선에 대한 잠재적인 효능을 가지고 있는 것으로 사료된다.

• 농업생명과학연구
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• 제44권3호
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• pp.61-67
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• 2010

국산 재배삼 4년근의 뿌리, 장뇌삼 4년근과 8년근의 잎과 뿌리에 대한 물 및 80% 에탄올 추출물에 대한 구성사포닌 및 항산화 활성을 분석하였다. 재배삼 80% 에탄올 추출물에 대한 조사포닌 함량은 3.85% (d.b) 이었으며, 4년근 장뇌삼 뿌리와 잎의 조사포닌 함량은 각각 6.75 및 8.57% (d.b)이었으며, 8년근 장뇌삼은 각각 6.53 및 7.54% (d.b)이었다. 재배삼의 구성사포닌은 ginsenoside-$Rh_1$의 함량이 6.07 mg/g으로 가장 높았고, 4년근 장뇌삼뿌리는 $-Rb_1$이 11.63 mg/g, 잎은 -Re가 24.35 mg/g으로 가장 높았고 8년근 장뇌삼 뿌리는 $-Rh_1$ 이 19.77 mg/g, 잎은 -Re가 20.43 mg/g으로 가장 높았다. 항산화활성 ($IC_{50}$값)은 삼의 종류 및 연근별로 1.84~21.88 mg/mL의 범위이었고 8년근 장뇌삼 잎 80% 에탄올 추출물에서 가장 높았다. 총 항산화력은 5.56~20.67 mg AA eq/g의 범위를 나타내었으며, 8년근 장뇌삼 잎 증류수 추출물에서 가장 높은 값을 나타내었다.

• Journal of Ginseng Research
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• 제45권2호
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• pp.246-253
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• 2021

Background: Ginseng is one of the most valuable herbal supplements. It is challenging to perform quality control of ginseng products due to the diversity of bioactive saponins in their composition. Acid or alkaline hydrolysis is often used for the structural elucidation of these saponins and sugars in their side chains. Complete transformation of the original ginsenosides into their aglycones during the hydrolysis is one of the ways to determine a total saponin group content. The main hurdle of this approach is the formation of various by-products that was reported by many authors. Methods: Separate HPLC assessment of the total protopanaxadiol, protopanaxatriol and ocotillol ginsenoside contents is a viable alternative to the determination of characteristic biomarkers of these saponin groups, such as ginsenoside Rf and pseudoginsenoside F₁₁, which are commonly used for authentication of P. ginseng Meyer and P. quinquefolius L. samples respectively. Moreover, total ginsenoside content is an ideal aggregated parameter for standardization and quality control of ginseng-based medicines, because it can be directly applied for saponin dosage calculation. Results: Different hydrolysis conditions were tested to develop accurate quantification method for the elucidation of total ginsenoside contents in herbal products. Linearity, limits of quantification, limits of detection, accuracy and precision were evaluated for the developed HPLC-MS method. Conclusion: Alkaline hydrolysis results in fewer by-products than sugar elimination in acidic conditions. An equimolar response, as a key parameter for quantification, was established for several major ginsenosides. The developed approach has shown acceptable results in the analysis of several different herbal products.

• 한국약용작물학회지
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• 제26권3호
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• pp.214-219
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• 2018

Background: This study was carried out to determine the best time for collecting ginseng berries without reducing the ginsenoside-Re content of ginseng roots, which are used as food, medicine, or cosmetic materials. Methods and Results: The test variety of ginseng used in this study was is Chunpung, which was collected from a 4-year-old ginseng field. Ginseng berries were collected at 7, 14, 21, 28, 35, 42, 49, and 56 days after flowering. The number of berry bunches per $1.62m^2$ ranged from 43.4 to 61.4, while the weight of berries per $1.62m^2$ was the greatest when they were collected 49 days after flowering. The root fresh weight per $1.62m^2$ was increased by 0.21 - 1.00 kg compared with that before the test, but root weight gain was decreased as the berry collection time was delayed. Total ginsenoside content of 4-year-old ginseng was the highest when berries were collected 7 days after flowering, while the ginsenoside-Re contents was the highest when collection was done 14 days after flowering. Conclusions: The most suitable period for ginseng berry collection was proposed to be from 14 to 21 days after flowering, as this is when the content of ginsenoside-Re, which is useful as a medicinal or cosmetic material, is still high and the ginseng root has not yet decreased in weight.

• Journal of Ginseng Research
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• 제38권1호
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• pp.22-27
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• 2014

Background: Research has been conducted with regard to the development of methods for improving the pharmaceutical effect of ginseng by conversion of ginsenosides, which are the major active components of ginseng, via high temperature or high-pressure processing. Methods: The present study sought to investigate the anticancer effect of heat-processed American ginseng (HAG) in human gastric cancer AGS cells with a focus on assessing the role of apoptosis as an important mechanistic element in its anticancer actions. Results and Conclusion: HAG significantly reduced the cancer cell proliferation, and the contents of ginsenosides Rb1 and Re were markedly decreased, whereas the peaks of less-polar ginsenosides [20(S,R)-Rg3, Rk1, and Rg5] were newly detected. Based on the activity-guided fractionation of HAG, ginsenoside 20(S)-Rg3 played a key role in inducing apoptosis in human gastric cancer AGS cells, and it was generated mainly from ginsenoside Rb1. Ginsenoside 20(S)-Rg3 induced apoptosis through activation of caspase-3, caspase-8, and caspase-9, as well as regulation of Bcl-2 and Bax expression. Taken together, these findings suggest that heat-processing serves as an increase in the antitumor activity of American ginseng in AGS cells, and ginsenoside 20(S)-Rg3, the active component produced by heat-processing, induces the activation of caspase-3, caspase-8, and caspase-9, which contributes to the apoptotic cell death.