• 한국약용작물학회:학술대회논문집
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• 2011.09a
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• pp.162-163
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• 2011

• 한국약용작물학회:학술대회논문집
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• 2011.04a
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• pp.388-389
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• 2011

• Journal of Microbiology and Biotechnology
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• v.10 no.2
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• pp.264-266
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• 2000

A new GFP-based T-vector for cloning of PCR products was developed by using a green fluorescent protein (GFP) as a mafker. In order to facilitate the DNA inserts, multiple restriction sites, SP6 and T7 RNA polymerase promoter sites, were introduced close to the PCR DNA insertion site of a pCRGv vector. The XcmI-digested pHNT plasmid can be used to clone a 3' A-overhanged PCR DNA amplified by Taq DNA polymerase. A potential method of easing some difficulties from its use along with its cost savings proveded by this vector are likely to lead to the replacement of other T-vectors for PCR DNA cloning.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.5
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• pp.1087-1091
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• 1999

Microwave extraction system equipped with closed vessels, which is known to rapidly extract target compounds from natural products, was applied to monitor the changes in phenolic compounds, browning color intensity and electron donating ability by using response surface methodology(RSM). Maximum content of phenolic compound was 21.65mg/100ml in 67.88% of ethanol concentration, 145oC of extraction temperature, and 6.24min of extraction time. The phenolic compounds in extracts are dependent on the increase of the extraction temperature and the ethanol concentration. Browning color intensity, which was maximized in 67.21%, 147oC, and 6.02min, was proportional to the increase of the extraction temperature. Maximum value of electron donating ability was 24.50units in 54.33%, 147oC, and 6.11 min. The electron donating ability of extracts was dependent on the increase of extraction temperature and maximized in the range from 50 to 65% of ethanol concentration.

• Journal of Environmental Health Sciences
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• v.10 no.1
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• pp.79-86
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• 1984

生長素를 制限한 培地에서 人蔘製品이 Aflatoxin 생산 菌株인 Aspergillus parasitius의 발육과 독소생산에 미치는 영향을 알기 위하여 본실험을 실시하였다. 공여된 균주는 미국 Wisconsin대학교 식품과학과 미생물 연구실에서 분주되었으며 독소생산에 최적인 30${\circ}C$에 7일간 배양하였다. 生長素의 減量과 인삼제품의 添加는 Aflatoxin의 생산능력을 저하시켰으나 발육에는 영향을 미치지 않았다. Aflatoxin $B_2$의 생산은 0.1%의 인삼 extract 첨가배지에서 그리고 Aflatoxin $G_1$은 3%의 인삼차 첨가배지에서 최고로 나타났으며, 0.5%의 인삼 extract 첨가배지에서는 Aflatoxin $B_1$과 $G_1$공히 최저로 나타났다. 균주의 포자형성은 인삼차 첨가배지에서 對照群과 인삼 extract 첨가배지 보다 2일 지연되어 나타났다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.5-5
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• 2010

Selection of specific medicinal sources as well as bioactive compounds is important for the preparation of medicine and related products with good quality. It is necessary to pay close attention for choosing correct medicinal sources, particularly in case of medicinal plants, because of their diversity, which can affect the quality and efficacy of medicine. Discrimination of plants based on morphological or genetic characteristics has been used as a conventional classification method of pharmaceutical sources so far; however, more need demands more general methods for accurate quality assessment of medicinal plants. In this study, ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) technique applied to this metabolic profiling is a powerful tool due to its higher sensitivity, resolution, and speed compared to conventional HPLC technique. The metabolite profiling of several medicinal plants including Panax ginseng was carried out using UPLC/Q-TOF MS and total metabolites were then subsequently applied to various statistical tools to compare the patterns. The developed metabolomics tool with UPLC/Q-TOF MS successfully identified and classified the samples tested according to their origins.

• Analytical Science and Technology
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• v.8 no.4
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• pp.655-661
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• 1995

The electrochemical reduction of coumarin derivatives in 0.1M TEAP acetonitrile solution was investigated by the direct current, differential pulse polarography, cyclic voltammetry and controlled potential coulometry. The electrochemical reduction of 7-acetoxy-4-bromomethyl-coumarin(ABMC) was proceeded as an irreversible three steps(-0.58, -1.63 and -2.25 volts) of electrochemical transfer before chemical reaction. The solution color turned to yellow after the carboxyl group was reduced at 2nd step(-1.63 volts vs. Ag-AgCl) and the change in color was independant to the bromo group. Upon the basis of the results on the products analysis and the interpretaton of polarograms, a possible electrochemical reaction mechanism was suggested.

• Proceedings of the Korea Information Processing Society Conference
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• 2015.10a
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• pp.945-948
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• 2015

본 연구는 비 정형 농산물 중 6년근 수삼의 자동 등급 분류하기 위한 선행연구로, 이를 위해 4방향에서 측정 가능케 하는 수삼 측정기를 제작하였으며, 측정된 수삼영상에서 뼈대와 몸통부분을 인식하기 위한 알고리즘을 고안하여 적용하였다. 적용 결과 6년 근 수삼에서 홍삼으로 만들어 졌을 때 가공 후 영상을 추정가능하다는 것을 보였다.

• Archives of Pharmacal Research
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• v.6 no.1
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• pp.63-68
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• 1983

The binding properties of three ginsenosides, Rb$_{1}$, Rc and Re, to bovine and human serum albumins have been examined by fluorescence probe technique. 1-anilinonphathalene-8-sulfonate (ANS) was used as the fluorescence probe. Protopanaxatriol glycoside, Re, did not quench the fluorscence of ANS to the bovine serum albumin. Competitive bindings between protopanaxadiol glycosides, Rb$_{1}$ and Rc are both 3.3 . The binding constants for Rb$_{1}$ and Rc with bovine serum albumin were 1.91 * 10$_{4}$M$_{-1}$ AND 1.04 * 10$^{[-994]}$ M$^{-1}$ , respectively. The ginsenosides, Rb$_{1}$, Rc and Re did not quench the fluorescence of ANS bound to human serum albumin.

• Korean Journal of Environmental Agriculture
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• v.31 no.3
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• pp.271-276
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• 2012

BACKGROUND: It is hard to control the agricultural products exceeding MRL (maximum residue limit) before forwarding. Therefore, NAQS (National Agricultural Products Quality Management Service, South Korea) established PHRL (pre-harvest residue limit) on agricultural products during their cultivation periods. This study was performed to set the PHRLs of peach during cultivation period, and also to estimate biological half-lives for residues of clothianidin and fluquinconazole. METHODS AND RESULTS: Two groups of peach were treated under Korean GAP (Good Agricultural Practices) with application time, single and triple treatments. Sample was collected over 14days (each after 0, 2, 4, 6, 8, 10, 12, 14 days. 8times), and clothianidin and fluquinconazole were analyzed by HPLC/DAD and GC/ECD, respectively. CONCLUSION: The biological half-life of clothianidin in single treatment and triple treatment was 5.2days and 7.0days. That of fluquinconazole was also 3.9days and 4.1days, respectively. The PHRL of peach on 10days before harvest was 1.4 mg/kg in clothianidin and 1.8 mg/kg in fluquinconazole.