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Effect of Gibberellin Treatment on Growth and Flowering Characteristics in the Cultivation of Aquilegia japonica Nakai & H. Hara

  • Oh, Hoon Geun;Lee, Joung Won;Lee, Gyeong Ja;Park, Jae Seong
    • Korean Journal of Plant Resources
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    • v.31 no.6
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    • pp.591-596
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    • 2018
  • This study was conducted to develop gibberellin treatment technique to enhance flower initiation in Aquilegia japonica Nakai & H. Hara. Seedlings were planted in 12cm-diameter pots on October 2016 and grown in green house. Ambient temperature in the green house was set at minimum $15^{\circ}C$ during day and night to suppress flower initiation at cold temperature condition. Two different types of gibberellin, $GA_3$ and $GA_{4+7}$, at 4 different concentration levels 100, 200, 400 and 600 mg/L, were tested in this study. Gibberellin was sprayed first at planting and secondly at 1-week after planting. Ten to fifteen ml of gibberellin was sprayed for each pot. Plant height and petiole length were elongated by both gibberellin types, flowering was more enhanced by $GA_3$ (91.7~100%) compared to of $GA_{4+7}$. However, abnormal flower was less observed in $GA_3$ treatment (0~16.7%) than $GA_{4+7}$. Number of flower stalks per plant ranged from 1.9 to 2.5. Number of flowers per plant ranged from 6.8 to 10.3. Differences in flowering characteristics between treatments were statistically significant. Optimal gibberellin treatment to enhance flower initiation in A. japonica Nakai & H. Hara substituting cold treatment was $GA_3$ at the concentration between 400 mg/L to 600 mg/L.

The Effect of Gibberellin on the Content of Vitamin C during the Growth of Mung Bean Sprout (녹두(綠豆)나물의 생육과정(生育過程) 중(中) Vitamin C 함량(含量)에 미치는 Gibberellin의 효과(效果))

  • Ko, Mu-Suk;Park, Bock-Hee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.10 no.1
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    • pp.117-122
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    • 1981
  • Mung bean sprout has been grown for 96 hours by soaking mung bean in such eight test divisions as Gibberellin 5-50ppm solution, well-water, and so on. Study on the charge of vitamin C an reducing sugar during its growth can be summarized as follows. 1. During the growth of mung bean sprout, the length and weight in the sample treated with Gibberellin 20ppm are highest of all Gibberellin divisions. 2. When mung bean sprout has grown for 24 hours, its vitamin C content reaches maximum in each sample. 3. There is little dehydro asscorbic acid in each sample during the growth period of 24-96 hours. 4. During the period of growth, the content of vitamin C in the sample treated with Gibberellin 5ppm are highest of all. 5. When mung bean sprout has been grown enough for cooking, in 48 hours, the content of vitamin C in the sample treated with Gibberellin 10ppm is highest but a significant difference is not recognized. 6. After 24 hours growth, vitamin C decreases but reducing sugar increases.

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The Effect of Gibberellin, Urea and Sucrose on Growth and Some Nutrients in Soybean Sprout (콩나물의 성장(成長) 및 성분(成分)에 미치는 Gibberellin, Urea 및 Sucrose의 영향(影響))

  • Kim, Dong-Yeon
    • Applied Biological Chemistry
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    • v.4
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    • pp.29-32
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    • 1963
  • The Gibberellin is applied to partial fruit trees, vegetables and flowers, but there is only a few information on the components of the effect of Gibberellin on these higher plants. There is no any information on the effect of Gibberellin, Urea and Sucrose mixed liquid on the plant components. So this mixed liquid and gibberellin spraied to the soybean sprout and observed growth velocity variety of some nutrients. Growth velocity, weight, Amino acid contents and fat contents of mixed plot and gibberellin plot are almost same. Mixed liquid plot's sugars and crude proteins contents in a little much than gibberellin plot, but mixed liquid plot's Ascorbic acid contents is a little less than gibberellin plot.

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Productivity test on some screened strains of Gibberella fujikuroi(Saw.) (Gibberellin 산류 생산균주의 분리와 생산성검토)

  • Lim, S.U.;Lee, C.Y.
    • Applied Biological Chemistry
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    • v.14 no.2
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    • pp.171-175
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    • 1971
  • Gibberella fujikuroi(imperfect stage Fusarium moniforme) a soil fungi is well known as the producer of plant growth regulator Gibberellins. The present work was planned for the isolation of the active strains of Gibberella fujikuroi from the native paddy soils. Twenty two strains were isolated from the infected rice seedlings collected from four local areas. Pyongtaek, Yesan, Tangjin and Sunchon and screened through the activity test for the production of Gibberellins. The strains P-105, Y-14 and T-58 yielded higher activity than the others isolated and the referred strain IAM-8048. The strains Y-5, Y-7, T-54 and S-152, however, were less promotive or rather inhibitory in the growth of rice seedlings. Six different kinds of culture media developed by Cross, Raulin-Thom, Borrow, West, Stodola and Kurosawa respectively were compared with each other for the production of Gibberellins and the best result was obtained with Raulin-Thom's media(glucose 16% and $NH_4NO_3$ 0.24%).

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Altered Fine Structure of Amylopectin Is Induced by Exogenous Gibberellin During Rice Grain Ripening

  • Kim, Sang-Kuk;Park, Shin-Young;Lee, Sang-Chul;Lee, In-Jung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.6
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    • pp.523-526
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    • 2006
  • When $GA_{4}$ was applied to heading stage, it was examined to understand the change of plant hormones and starch during grain filling and ripening. Exogenous gibberellin caused a dramatic decrease in endogenous ABA content. Endogenous $GA_{4}$ content in both superior and inferior part was more promoted in $GA_{4}-treated$ rice grain than in the control. $GA_{1}$ content of an inferior part was not detected in the control and $GA_{4}-treated$ rice otherwise $GA_{4}$ was detected in all grain parts. Ripened grain rate in $GA_{4}-treated$ rice grain was lower than that of the control plant. Amylopectin from $GA_{4}-treated$ grain contained more very short chains with degree of polymerization (DP) between 4 and 8 than amylopectin from the control plant. It suggests strongly that fine structure of rice endosperm may be changed by exogenously applied $GA_{4}$ in rice plants.

Seed Dormancy and Germination Characteristics of Endemic Elder Species (Sambucus racemosa subsp. pendula) and Common Elder Species (S. williamsii) in Korea

  • Hyo-In, Lim
    • Journal of Forest and Environmental Science
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    • v.38 no.4
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    • pp.284-289
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    • 2022
  • This study was conducted to determine the seed dormancy types of Sambucus racemosa subsp. pendula Nakai and S. williamsii Hance. Low temperature stratification (1, 2, 3, 4 months) and GA3 treatment (1,000 mg/L) were performed on seeds to determine the type of seed dormancy. After the treatment, seeds were placed on a petri dish at 25℃ under light conditions. The germination rate and mean germination time were investigated. Results showed that cold stratification was effective in breaking the dormant state of the seed in both species. In the low temperature stratification treatment, the seed germination performance was improved as the treatment period was prolonged. Gibberellin treatment was effective in breaking the dormant state of S. racemosa subsp. pendula without low temperature stratification. However, S. williamsii did not break the dormant state of the seed by gibberellin treatment without low temperature stratification treatment. In the gibberellin treatment, germination performance was improved according to the low temperature stratification treatment period. As a result of this study, the seeds of S. williamsii have both an intermediate complex and a deep complex morphophysiological dormancy (MPD). In comparison, it was found that the S. racemosa subsp. pendula had intermediate composite MPD.

Cultural Environments on Growth and Tuberlet Yield of Pinellia ternata(Thunb.) Breit (반하의 재배환경에 따른 생육 및 수량)

  • Kim, Young-Jin;Park, Moon-Soo;Park, Ho-Ki;Kim, Sun;Kim, Tai-Soo;Chang, Young-Sun
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.3
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    • pp.240-245
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    • 1995
  • This study was conducted to find out environment conditions in order to increase of growth and yield of tuberlets in pinellia ternata (Thunb.) Breit. The optimal temperature conditions for the growth of tuberlets were good rather temperature fluctuations than constant one and the optimal photoperiod was a 6hr-photoperiod. The growth of tuberlets to the kinds and concentration of growth regulators was good for 0. lppm 2.4- D, 10ppm kinetin and 50ppm gibberellin treatment. In colchicine soaking treatment, the growth of tuberlets was bad, but bulblets formation was good for 0. 05% colchicine treatment for 48hr. -soaking. In colchicine dropping treatment, dry tuber yield was good for 0. 1% colchicine treatment with once dropping for one day.

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Effects of Plant Growth Regulators on Growth of Rice Seedling for Machine Transplanting (식물생장조절제 처리가 벼기계이앙 어린모 생육에 미치는 영향)

  • 이주열
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.4
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    • pp.360-365
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    • 1993
  • The infant seedling of rice(8-to 10-day-old) has often suffered from submergence just after transplanting due to the too short seedling height, 5 to 8cm, This experiment was conducted to elucidate the effect of gibberellic acid($GA_3 : 25% soluble liquid) with the application methods of foliar spray and seed-soaking on the elongation of seedling height of infant rice seedling in machine transplanting, We used a japonica rice variety, Hwaseongbyeo, and the seeding rate was 200g per seed tray(30$\times60$\times3cm). Generally,$GA_3 and Metalaxyl increased the elongation of seedling height of infant seedling while Tetracycle decreased the seedling height, To increase the seedling height of infant seedling, the foliar spary application of $GA_3 was better than seed-soaking treatment. The optimum concentration of $GA_3 for the foliar spray was about l0ppm and the optimum application time of $GA_3 was 3 to 4 days after sowing.

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Germination Ability of Pregerminated and Dried Rice Seed (벼 최아후 건조종자의 발아능력)

  • Kwon, Tae-Oh;Cheong, Jin-Il;Noh, Tae-Hwan;Namkoong, Seung-Bak
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.3
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    • pp.317-322
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    • 1997
  • In order to investigate vigor of rice seed pregerminated, some organic and inorganic component content, response of ecotypes, and effect of gibberellin treatment, under different levels of hastening of germination, were examined. Protein content was high in dried seed, the larger size in length of pregerminated seed, the less protein content, but lipid content was a reverse tendency to protein one. Potassium content was low in dried seed, the larger size in length of pregerminated seed, the more potassium content, but increase dramatically at above 10 mm in length of sprout. Magnesium content showed a same tendency to potassium one. The ability of regermination was high in early maturing cultivar, but almost lost in the pregerminated seeds with above 5 mm in length of sprout. The regermination percentage of dried seed was lower than that of pregerminated seed with 0.1∼0.5 mm in length, but was similar to that of pregerminated one with 1∼3 mm in length of sprout. The effect of gibberellin on regermination percentage was highest at 10 ppm and showed high with small sized one of pregerminated seed. The effect of gibberellin on plant height, fresh weight, and dry weight showed a same tendency to regermination percentage.

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Phoma herbarum as a New Gibberellin-Producing and Plant Growth-Promoting Fungus

  • Hamayun, Muhammad;Khan, Sumera Afzal;Khan, Abdul Latif;Rehman, Gauhar;Sohn, Eun-Young;Shah, Aamer Ali;Kim, Sang-Kuk;Joo, Gil-Jae;Lee, In-Jung
    • Journal of Microbiology and Biotechnology
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    • v.19 no.10
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    • pp.1244-1249
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    • 2009
  • Endophytic fungi are known for the production of valuable metabolites, but information on the gibberellin production capacity of this group is limited. We isolated 9 endophytic fungi from the roots of salt-stressed soybean plants and screened them on waito-c rice, in order to identify plant growth promoting fungal strains. The fungal isolate TK-2-4 gave maximum plant length (20.35 cm) promotion in comparison with wild-type Gibberella fujikuroi (19.5 cm). In a separate experiment, bioassay of TK-2-4 promoted plant length and biomass of soybean cultivar Taegwangkong. The TK-2-4 culture filtrate was analyzed for the presence of gibberellins, and it was found that all physiologically active gibberellins, especially $GA_4$ and $GA_7$, were present in higher amounts ($GA_1$, 0.11 ng/ml; $GA_3$, 2.91 ng/ml; $GA_4$, 3.21 ng/ml; and $GA_7$, 1.4 ng/ml) in conjunction with physiologically inactive $GA_9$ (0.05 ng/ml), $GA_{12}$ (0.23 ng/ ml), $GA_{15}$ (0.42 ng/ml), $GA_{19}$ (0.53 ng/ml), and $GA_{20}$ (0.06 ng/ml). The fungal isolate TK-2-4 was later identified as a new strain of Phoma herbarum, through the phylogenetic analysis of 28S rDNA sequence.