• 제목/요약/키워드: ghosts

검색결과 68건 처리시간 0.019초

플라스미드 유전자 함유 혈구 세포 입자의 제조 (Encapsulation of Plasmid DNA in Erythrocyte Ghosts)

  • 변향민;박상은;김정목;고정재;오유경
    • Journal of Pharmaceutical Investigation
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    • 제32권3호
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    • pp.181-184
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    • 2002
  • This study reports the encapsulation of plasmid DNA in erythrocyte ghosts. The plasmid DNA was encapsulated into erythrocyte ghosts using three methods; osmotic shock, electroporation in isotonic medium, and e1ectroporation in hypotonic medium. Of three methods, electroporation in hypotonic medium resulted in the highest encapsulation efficiency of plasmid DNA. The morphology of erythrocyte ghosts prepared by electroporation in hypotonic medium was similar to that by osmotic shock alone. The circulation time of plasmid DNA in mice was prolonged by administration in erythrocyte ghost-encapsulated forms. These results indicated the potential of erythrocyte ghosts for biocompative nonviral delivery system of therapeutic genes for hematological diseases.

플라즈마 단백질의 고스트 적혈구 흡착 특성 (Adsorptive Binding of Plasma Proteins to Red Blood Cell Ghosts)

  • 김동욱;차운오
    • KSBB Journal
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    • 제11권6호
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    • pp.630-635
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    • 1996
  • IgG와 알부민이 고스트 적혈구의 분산하에서 적 혈구의 세포막에 흡착되었다. 용액중 IgG의 농도가 0 O.1mg/mL일 때, 고스트 적혈구의 부피비가 5% 에 서 45%로 증가함에 따라 흡착으로 인한 용액중의 IgG의 농도감소는 14%에서 45%로 증가하였고, h hardened 고스트 척혈구 분산시가 고스트 적혈구 분산시보다 더 많은 단백질이 흡착되었다. 용액중 알부민의 농도가 0.075mg/mL일 때, 고스트 적헐구 의 부피가 5%에셔 70%로 증가함에 따라 용액중의 얄부민의 농도감소는 12% 에서 47%로 증가하였다. 흡착시간에 대한 IgG의 농도변화를 측정한 결과 단 백질의 흡착은 빠른 속도로 일어남을 알 수 있었고, 적혈구당 흡착된 단백질 분자수를 계산한 결과 용액 내 적혈구의 수가 증가할수록 적혈구당 흡착된 분자 수는 감소하였다.

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Respiratory burst activity of tilapia (Oreochromis mosambicus) phagocytes to Edwardsiella tarda ghosts

  • 김기홍;권세련;남윤권;이은혜;김민선
    • 한국어병학회지
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    • 제19권3호
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    • pp.285-288
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    • 2006
  • The respiratory burst activity of tilapia (Oreochromis mosambicus) phagocytes agaisnt Edwardsiella tarda ghosts produced by gene E mediated lysis was investigated. Stimulation with E. tarda ghosts (ETG) showed markedly higher respiratory burst activity than that with formalin killed E. tarda (FKC) in phagocytes. This result may suggest that ETG can induce effective cellular immune responses in fish.

Multipath Ghosts in Through-the-Wall Radar Imaging: Challenges and Solutions

  • Abdalla, Abdi T.;Alkhodary, Mohammad T.;Muqaibel, Ali H.
    • ETRI Journal
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    • 제40권3호
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    • pp.376-388
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    • 2018
  • In through-the-wall radar imaging (TWRI), the presence of front and side walls causes multipath propagation, which creates fake targets called multipath ghosts. They populate the scene and reduce the probability of correct target detection, classification, and localization. In modern TWRI, specular multipath exploitation has received considerable attention for reducing the effects of multipath ghosts. However, this exploitation is challenged by the requirements of the reflecting geometry, which is not always available. Currently, the demand for a high radar image resolution dictates the use of a large aperture and wide bandwidth. This results in a large amount of data. To tackle this problem, compressive sensing (CS) is applied to TWRI. With CS, only a fraction of the data are used to produce a high-quality image, provided that the scene is sparse. However, owing to multipath ghosts, the scene sparsity is highly deteriorated; hence, the performance of the CS algorithms is compromised. This paper presents and discusses the adverse effects of multipath ghosts in TWRI. It describes the physical formation of ghosts, their challenges, and existing suppression techniques.

Erythrocyte Ghost의 광산화반응에 미치는 인삼추출물의 영향 (Effect of Ginseng Extracts on Photosensitized Peroxidation of Human Erythrocyte Ghosts)

  • 백태홍;천현자;강병수
    • Journal of Ginseng Research
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    • 제14권1호
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    • pp.30-35
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    • 1990
  • The photooxidation of human erythrocyte ghosts has been studied and the effects of ginseng water extract on it have been investigated. In the presence of photosensitizer, rose bengal, human erythrocyte ghosts have caused photooxidation by lO2 and produced lip)id hydroperoxides. Ginseng water extract and d-${\alpha}$-tocofherol have inhibited photooxidation, ,whereas ascorbic acid has developed in low concentrations but inhibited in high concentrations. On the other hand, amounts of lipid hydroperoxide produced by photooxidation were decreased by addition of catalase after irradiation and according to sequential addition of ginseng water extract into it, the formation of lipid hydrolleroxide was decreased simultaneously.

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Comparison of the immunogenicity between bacterial ghost and formalin-killed bacteria for Vibrio vulnificus

  • Kwon, Se Ryun
    • 한국어병학회지
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    • 제25권3호
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    • pp.159-164
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    • 2012
  • Vibrio vulnificus ghosts (VVG) were generated using a mobilizable vector including a thermosensitive expression cassette by conjugation. The vaccine potential of VVG was investigated in mouse. Mice immunized with VVG showed significantly higher antibody titer than those with formalin-killed V. vulnificus. The present study supports the conceptive usefulness of bacterial ghosts as vaccine candidates.

어류 병원성 세균 Edwardsiella tarda의 형질전환 및 재조합 ghost 세균에서의 E-lysis 유전자의 전사 발현 특징 (Transformation of Edwardsiella tarda and Transcriptional Characteristics of E-lysis Gene in Recombinant Bacterial Ghosts)

  • 권세련;남윤권
    • 한국어류학회지
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    • 제19권2호
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    • pp.83-87
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    • 2007
  • 어류 에드워드감염증에 대한 예방 재조합 ghost 백신을 개발하기 위한 연구의 일환으로 어류 병원성 세균인 Edwardsiella trada를 대상으로 플라스미드 형질전환을 실시하고 형질전환 안정성을 평가하였으며, 형질 도입된 재조합 ghost 세균의 E-lysis 유전자 발현을 분석하였다. E. tarda를 대상으로 한 ghost 유도는 대장균에 비해 상대적으로 장시간의 반응 시간이 요구되며 lysis의 개시가 지연된 점을 고려 시 발현된 E protein의 용해 능력 또는 E-gene의 전사발현 양이 E. tarda에서 다소 약화되는 것으로 나타났다. 그러나 대장균에 비해 ghost 유도 속도가 다소 낮음에도 불구하고 반응이 완성되었을 시점에서의 E. tarda의 ghost 효율은 대장균과 전혀 차이가 없이 99.99% 이상의 유도효율을 나타내었다.

Paranormal Beliefs: Using Survey Trends from the USA to Suggest a New Area of Research in Asia

  • Kim, Jibum;Wang, Cory;Nunez, Nick;Kim, Sori;Smith, Tom W.;Sahgal, Neha
    • Asian Journal for Public Opinion Research
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    • 제2권4호
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    • pp.279-306
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    • 2015
  • Americans continue to have beliefs in the paranormal, for example in UFOs, ghosts, haunted houses, and clairvoyance. Yet, to date there has not been a systematic gathering of data on popular beliefs about the paranormal, and the question of whether or not there is a convincing trend in beliefs about the paranormal remains to be explored. Public opinion polling on paranormal beliefs shows that these beliefs have remained stable over time, and in some cases have in fact increased. Beliefs in ghosts (25% in 1990 to 32% in 2005) and haunted houses (29% in 1990, 37% in 2001) have all increased while beliefs in clairvoyance (26% in 1990 and 2005) and astrology as scientific (31% in 2006, 32% in 2014) have remained stable. Belief in UFOs (50%) is highest among all paranormal beliefs. Our findings show that people continue to hold beliefs about the paranormal despite their lack of grounding in science or religion.

유아의 성, 연령, 정서행동문제 수준에 따른 유아기 두려움 목록 분석 (Analysis of the Fear Inventory by Preschoolers' Sex, Age, and Degree of Emotional and Behavioral Problems)

  • 구미향
    • 아동학회지
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    • 제30권3호
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    • pp.55-69
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    • 2009
  • The purpose of this study was to describe preschoolers' perceptions of fear from their own perspective. Qualitative data from 213 preschoolers 4 to 6 years of age were obtained by semi-structured interviews. The fear inventory was analyzed by content analysis and frequency. The most common fears of preschoolers in this study were fear of ferocious animals and imaginary figures, such as tigers, lions, snakes, spirits and ghosts. Fear of ghosts was the primary fear of both boys and girls. Results showed that most fears were caused by disgust-related visual images. Worry about injury and pain was another common pathway to fear. At-risk preschoolers scored significantly higher than normal preschoolers on fears of criticism and getting an injection.

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Joint 채널등화 알고리즘 개발 및 8-VSB HDTV 수신시스템에의 응용 (Development of a Joint Equalization Algorithm and Its Applications to 8-VSB HDTV Receiver)

  • 박경도;황유모
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1998년도 하계학술대회 논문집 G
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    • pp.2424-2426
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    • 1998
  • We propose a robust equalization algorithm to improve the performance of airplane flutter casuing dynamic ghosts. The algorithm is an augumentation of the DFE with training sequence(DFE-TS) with the Modified Stop-and-Go Algorithm(MSGA) based on DFE structure, which is called a DFE-TS/MSGA. This will allow the equalizer to switch itself back to blind mode almost instantaneously when drastic and sudden changes in the channel occur. Test results based on the 8-VSB HDTV receiver show that the proposed algorithm is robust against dynamic ghosts and outperforms the conventional DFE-TS in reducing the intersymbol interference.

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