• 한국육종학회지
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• 제42권5호
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• pp.448-454
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• 2010

The objective of this study was to rapidly evaluate fatty acids in a collection of foxtail millet (Setaria italica (L.) P. Beauv) of different origins so that this information could be disseminated to breeders to advance germplasm use and breeding. To develop the calibration equations for rapid and nondestructive evaluation of fatty acid content, near-infrared reflectance spectroscopy (NIRs) spectra (1104-2494 nm) of samples ground into flour (n=100) were obtained using a dispersive spectrometer. A modified partial least-squares model was developed to predict each component. For foxtail millet germplasm, our models returned coefficients of determination ($R^2$) of 0.91, 0.89, 0.98 and 0.98 for strearic acid, oleic acid, linoleic acid, and total fatty acids, respectively. The prediction of the external validation set (n=10) showed significant correlation between references values and NIRs values ($r^2=0.97$, 0.91, 0.99 for oleic, linoleic, and total fatty acids, respectively). Standard deviation/standard error of cross-validation (SD/SECV) values were greater than 3 (3.11, 5.45, and 7.50 for oleic, linoleic, and total fatty acids, respectively). These results indicate that these NIRs equations are functional for the mass screening and rapid quantification of the oleic, linolenic, and total fatty acids characterizing foxtail millet germplasm. Among the samples, IT153491 showed an especially high content of fatty acids ($84.06mg\;g^{-1}$), whereas IT188096 had a very low content ($29.92mg\;g^{-1}$).

• 한국작물학회지
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• 제60권1호
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• pp.75-84
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• 2015

Sesame (Sesamum indicum L.) is one of the most important oilseed crops with high oil contents and rich nutrient value. The development of a core collection could facilitate easier access to sesame genetic resources for their use in crop improvement programs and simplify the genebank management. The present study was initiated to the development and evaluation of a core collection of sesame based on 5 qualitative and 10 quantitative trait descriptors on 2,751 sesame accessions. The accessions were different countries of origin. About 10.1 percent of accessions were selected by using the power core program to constitute a core collection consisting of 278 accessions. Mean comparisons using t-test, Nei's diversity index of 10 morphological descriptors and correlation coefficients among traits indicated that the existing genetic variation for these traits in the entire collection has been preserved in the core collection. The results from this study will provide effective information for future germplasm conservation and improvement programs in sesame.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.70-70
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• 2017

This study was conducted to characterize the amylose and protein contents of 4,948 rice landrace germplasm using the NIRS model developed in the previous study. The amylose contents estimated by NIRS in the standard rice were Sinseonchal (6.881%) 4.994%, Chucheong (19.731%) 18.633%, Goami (23.246%) 20.548%. Protein contents were Sinseonchal (6.890%) 6.824%, Chucheong (6.350%) 6.869%, Goami (6.777%) 7.839%. The NIRS analysis showed that 1.1-2.7%point lower in amylose and 0.4-0.6%point higher in protein than standard contents. The average amylose content of the germplasm was 20.39% with a range of 3.97-37.13%. The average protein content was 8.17% with a range of 5.20-17.45%. Amylose contents with a range of 20.06-27.02% represented 62.20% of the germplasm. Protein contents with a range of 6.78-9.75% represented 81.60% of the germplasm. Korean landrace comprised 24.9% among the 4,948 germplasm collected from 41 countries. A specific range of amylose contents showed in Korea 16.58-20.06%, in Japan 20.06-23.25%, in North Korea 23.25-27.02% and in China 27.02-37.13%. Protein contents exhibited 5.20-17.45% evenly in the whole landraces, whereas Chinese landrace particularly observed with 6.78-8.27% and 9.75-17.45%. Fifty resources were selected with low and high amylose ranging from 3.97-6.66% to 30.41-37.13% respectively. Similarly fifty resources were selected with low and high protein ranging from 5.20-6.09% to 13.21-17.45% respectively. Landraces with higher protein should be adapted to practical utilization of food sources.

• 한국자원식물학회지
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• 제29권4호
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• pp.479-494
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• 2016

기계수확에 적합하고 고기능성 유전자원을 선발하여 중부지역에 재배품종자원으로 공급하기 위하여 수수 100 자원을 공시하여 실시한 시험결과는 다음과 같다. 황금찰과 남풍찰을 대조 품종으로 하여 수집된 수수 100 자원의 작물학적 특성을 검정한 결과 출수소요일은 68~94일의 변이로 변이가 컸으며, 이삭형태는 7종류의 형태로 중간형이 가장 많이 나타났다. 간장은 70~429 ㎝의 변이로 변이 폭이 컸으며, 평균 260 ㎝였고, 100~150 ㎝가 31%로 최빈수였다. 이삭길이는 17~44.4 ㎝의 변이를 보였으며, 평균은 28.8 ㎝였다. 경태는 16.6~29.7 ㎜의 변이로 변이 폭이 컸으며, 평균은 21.4 ㎜였고, 엽수는 6.3~20개의 변이로 변이 폭이 컸으며, 평균은 11.4개였다. 천립중은 14.55~32.54 g의 변이로 변이 폭이 컸으며, 평균은 22.14 g이었고, 이삭당 수량은 18.32~44.96 g의 변이로 변이 폭이 컸으며, 평균은 30.74 g이었다. 작물학적 특성 검정을 통해 기계수확에 적합한 150 ㎝ 이하의 단간종이며, 이삭당 수량이 대조품종보다 높은 33.63 g 이상인 18 유전자원을 선발하였다. 선발된 18 유전자원 중 기능성물질 함량이 높은 자원 선발을 위하여 총 폴리페놀 함량, 전자공여능 및 총 안토시아닌 함량을 분석한 결과, 총 폴리페놀 함량은 대조품종인 황금찰은 174.08 ㎍ GAE/g이었으며, 남풍찰은 292.30 ㎍ GAE/g이었고, DS017, DS019, DS020, DS027, DS066이 대조품종인 남풍찰보다 함량이 높았다. 전자공여능은 대조품종인 황금찰이 26.25%였으며, 남풍찰은 33.65%였고, DS017, DS019, DS020, DS027, DS066, DS068, DS236이 대조품종인 남풍찰보다 소거능이 높거나 유사하였다. 총 안토시아닌 함량은 대조품종인 황금찰은 49.22 ㎍/g이었으며, 남풍찰은 51.52 ㎍/g이었고, DS488, DS428이 대조품종인 남풍찰과 황금찰보다 함량이 높거나 유사하였다. 그리고 항산화능과 항산화 성분과의 상관관계를 선형회귀분석 한 결과, 총 폴리페놀 함량과 DPPH radical 소거능 사이에 99.9% 수준으로 R=0.922의 높은 상관관계가 나타나 총 폴리페놀 함량이 높을수록 DPPH radical 소거능이 증가함을 알 수 있었다. 작물학적 특성 검정과 기능성물질 함량분석을 통하여 기계수확에 적합한 단간종이며, 이삭당 수량이 높고, 기능성물질 함량이 높은 DS017, DS019, DS020, DS027, DS066 5자원을 선발하였다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.43-43
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• 2019

The collection, evaluation and conservation of crop germplasm have been treated as one of the basics to breeding program. An understanding of genetic relationships among germplasm resources is vital for future breeding process like yield, quality, and resistance. In the present study, EST-SSR markers were employed to assess the polymorphism and genetic diversity of 192 accessions of Proso millet preserved in the National Agrobiodiversity Center of RDA. We evaluated the efficiency of EST-SSR markers developed for proso millet species. A total of 98 alleles were detected with an average allele number of 4.5 per locus among 192 proso millet millet accessions using 22 EST-SSR markers. The averaged values of gene diversity ($H_E$) and polymorphism information content (PIC) for each EST-SSR marker were 0.362 and 0.404 within populations, respectively. Our results showed the moderate level of the molecular diversity among the proso millet accessions from diverse countries. A phylogenetic tree revealed three major groups of accessions that did not correspond with geographical distribution patterns with a few exceptions. The less correlation between the clusters and their geographic location might be considered due to their type difference. Our study provided a better understanding of genetic relationships among various germplasm collections, and it could contribute to more efficient utilization of valuable genetic resources. The EST-SSR markers developed here will serve as a valuable resource for genetic studies, like linkage mapping, diversity analysis, quantitative trait locus/association mapping, and molecular breeding.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1999년도 춘계 학술대회지
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• pp.96-97
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• 1999

• 한국유기농업학회지
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• 제19권spc호
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• pp.230-233
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• 2011

We collected and examined germplasm resources of lettuces, edible chrysanthemums and mallows for organic farmers. We found that 3 genotypes (OL-51, OL-52, and OL-63) are suitable for organic cultivation. The main use of treatment for grey mold among eco-friendly pest control methods is helpful to increase efficiency of seed-gathering after collecting and evaluating a total of 64 kinds of lettuces-collected 43 South Korea local varieties, 8 kinds cultivated by the Highland Agriculture Research Center, 4 kinds of cultivated lettuces, and 9 kinds for sales- to choose appropriate kinds of lettuces for organic cultivation in South Korea. We chose those with more than 300 leaves (OC-18, OC-44, and OC-51) after evaluation of yield characteristics of a total of 62 edible chrysanthemums. We covered each genotype of seed of edible chrysanthemums when gathering the seeds by ourselves so as not to decrease the purity of each genotype. We chosed OM-38, OM-39, and OM-40 for their yield performance and germination rates among the total 41 genotypes of mallows. We selected 9 genotypes of lettuces, editable chrysanthemums and mallows with possibility of organic cultivation and found that they can be used permanently through self seed-gathering on organic farms.

• 원예과학기술지
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• 제28권5호
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• pp.802-809
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• 2010

역병은 세계적으로 고추에 발생하는 병 중 가장 문제시 되는 병해로 국내 83개 지역에서 채집한 재래종 고추 유전자원 중 300개를 선발하여 공시하였다. 파종 후 온실에서 70일간 육묘하였고 공시한 역병균의 유주자낭 농도를 $10^5/mL$으로 하여 주당 5mL 관주접종 후, 습실 처리하였고 1주 간격으로 4주간 이병주율을 조사하였다. 접종 7일 후 이병주율을 조사하였더니, 공시한 300개 고추 자원 중 67개 자원에서 이병주율이 60.1% 이상으로 높은 감수성을 보였으며, 37개 자원은 접종 후 7일까지 전혀 발병하지 않았다. 역병균 접종 28일 후, 공시한 300점의 고추자원 중 244개 자원은 60% 이상의 이병주율을 보여 역병에 대해 감수성을 나타내었으며, 11개 자원은 20% 미만의 이병주율을 보여 중도저항성을 보였고, 5개 자원은 전혀 발병하지 않아 저항성을 나타내었다. 5개 자원의 저항성 고추유전자원은 역병저항성 고추 품종 육종에 유용한 중간모본으로 사용될 것으로 생각된다. 본 실험의 정밀도를 높이기 위해 분자마커를 활용한 추가실험이 진행된다면 고추유전자원의 역병 저항성에 대한 중요한 정보를 제공할 수 있을 것으로 본다.

• 한국자원식물학회지
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• 제34권6호
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• pp.575-599
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• 2021

The edible roots of radish (Raphanus sativus L.) are consumed worldwide. For characterization and evaluation of the agronomic traits and health-promoting chemicals in radish germplasms, new germplasm breeding materials need to be identified. The objectives of this study were to evaluate the phenotypic traits and glucosinolate contents of radish roots from 110 germplasms, by analyzing correlations between 10 quantitative phenotypic traits and the individual and total contents of five glucosinolates. Phenotypic characterization was performed based on descriptors from the UPOV and IBPGR, and glucosinolate contents were analyzed using liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode (MRM). Regarding the phenotypic traits, a significant correlation between leaf length and root weight was observed. Glucoraphasatin was the main glucosinolate, accounting for an average of 71% of the total glucosinolates in the germplasms; moreover, its content was significantly correlated with that of glucoerucin, its precursor. Principal component analysis indicated that the 110 germplasms could be divided into five groups based on their glucosinolate contents. High levels of free-radical scavenging activity (DPPH) were observed in red radishes. These results shed light on the beneficial traits that could be targeted by breeders, and could also promote diet diversification by demonstrating the health benefits of various germplasms.

• 식품보건융합연구
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• 제5권3호
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• pp.1-5
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• 2019

The field experiment was conducted in vegetable area, Institute of Horticultural Sciences in University of Agriculture, Faisalabad in order to recognize morphological and fruit growth pattern and yield of Tinda (Praecitrullus fistulosus) gourd germplasm lines. Sixteen germplasm lines in which one line is used as check are included were sown and grown on flat beds in field. The field experiment was arranged as randomized complete block design (RCBD) with three replications. Data on days to maturity, fruit per vine, fruit weight in grams, fruit diameter and total yield obtained were recorded. The fruit texture is marked as phenotype parameter. All parameters were collected and then analyzed statistically. All lines and replications showed different results among each other according to parameter. The maximum DTM (days to maturity), F/P (fruit per plant), FW (fruit weight), FD (fruit diameter) and Yield were recorded was $L_0$ (68.66), $L_3$ (1.66), $L_{10}$ (248.33), $L_{13}$ (8.50) and $L_0$ (599.33) and the minimum were recorded was $L_1$ (56), $L_{11}$ (0.33), $L_0$ (198), $L_9$ (7) and $L_4$ (421) grams respectively. All lines showed smooth texture of fruits with no hairs when mature at harvesting stage.