• Title/Summary/Keyword: germination rates

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Ecological relationship between soil-borne plant pathogens and rhizosphere microorganisms. I. Effects of Pseudomonas putida on the suppression of microconidia and chlamydospore germination of Fusarium oxysporum f. sp. cucumerinum (토양전염성 식물병원균과 근권미생물의 생태학적인 관계 I. Pseudomonas putida에 의한 오이덩굴쪼김병균(Fusarium oxysporum f. sp. cucumerinum)의 생장억제에 관하여)

  • Park Chang-Seuk;Choi Jin-Sik
    • Korean journal of applied entomology
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    • v.22 no.3 s.56
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    • pp.186-192
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    • 1983
  • The growth of germ tube of Fusarium oxysporum f. sp. cucumerinum was remarkably inhibited on the water agar treated with 100ppm of Fe-EDDHA, a synthetic iron chelating agent, whereas germination rate of microconidia did not show much differences compare with that of non treated water agar. Both of the germination and the germ tube elongation of microconidia were suppressed significantly in King's B agar by the bacterial siderophores produced by Pseudomonas putida. The highest germination of the chlamydospores was obtained in the soil added with $0.25\%$ of glucose plus $0.05\%$ of asparagine. The chlamydospores of cucumber wil fungus germinated about $14\%$ in rhizosphere soil of 2 day-old cucumber seedlings within 48 hours, and the germination was enhanced notably in rhizosphere soil of 10 day-old seedling. But the rates of germination was not increased according to cucumber growth age after 10 day-old seedling. The effect of P. putida and Fe-EDDHA on the germination on chlamydospores in conducive soil was not pronounced in the non-rhizosphere soil added with nutrient. However, the germination was suppressed significantly both in rhizosphere soil and in rhizosphere soil added with nutrient. The suppression of chlamydospore germination was greater in the bacteria inoculated soil than that in Fe-EDDHA treated soil.

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Changes in Chemical Components of Pigmented Rice during Germination (유색미 품종의 발아 시 화학성분의 변화)

  • Park, Young-hie;Kim, Bo Ra;Ryu, Su-Noh
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.63 no.1
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    • pp.18-24
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    • 2018
  • This study was conducted to use fundamental data about functional foods based on pigmented rice varieties-2 types of normal rice, 6 types of black rice-to analyze changes in chemical component activity during germination. The results are summarized as follows: Germination rates of the pigmented rice varieties were the highest at $30^{\circ}C$. There was a tendency for crude protein contents to increase under the influence of germination periods, and the crude protein contents were the highest on the $6^{th}$ day. Crude fat contents were the highest on days 6-8. Nevertheless, the crude fat contents in pigmented rice decreased during the first 2 days, and subsequently, the contents increased steadily. Crude fiber contents were the highest on the $8^{th}$ day. However, there was no meaningful tendency. Sucrose, glucose, and fructose contents in the pigmented rice increased during germination. In normal rice, glucose content increased up to the $8^{th}$ day. It was higher than that of brown rice. However, sucrose and fructose contents showed no meaningful tendency. There was a general tendency for GABA, alanine, glutamic acid, asparagine, aspartic acid, and lysine contents in the pigmented rice to increase under the influence of germination periods. Aspartic acid and asparagine contents were shown to decrease, compared with that in pre-germinated normal rice. In all varieties, lysine was detected from the second germination day onwards, and on the $6^{th}$ day after germination, its contents were 6.24 mg/100 g and 15.58 mg/100 g in normal rice and pigmented rice, respectively.

Relationships among Viviporous Germination, Dry-heat Tolerance and Seed Longevity in Milyang 23/Gihobyeo RILs (벼 밀양 23호$\times$기호벼의 재조합 자식계통에서 수발아성,건열 저항성 및 종자수명간 상관관계)

  • Lee, Seung-Yeob;Ahn, Jeong-Ho;Kim, Hyo-Jin;Bae, Jong-Hyang;Eun, Moo-Young
    • Journal of Bio-Environment Control
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    • v.15 no.4
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    • pp.421-427
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    • 2006
  • To investigate the relationships among the traits related seed dormancy in rice, viviparous germination, dry-heat tolerance and seed longevity were tested using 164 M/G RILs ($F_{17}$) derived from the cross between Milyang 23 (Indica/Japonica) and Gihobyeo (Japonica). At 40 days after heading, the viviparous germination rates of Milyang 23 and Gihobyeo were 0.0 and 7.0%, respectively. The averaged viviparous germination rate of 164 MIG RILs was 3.2%, and their range was from 0.0 to 50.9%. In dry-heat treatment at $90^{\circ}C$ for 24 h followed by the pre-drying at $40^{\circ}C$ for 2 days, Milyang 23 showed higher germinability (98.6%,2.7 days to $T_{50}$) than Gihobyeo (37.6%, 12.9 days to $T_{50}$), and the mean germination rate of 164 M/G RILs was 72.4%. In 54 months after harvest, the germinability in Milyang 23 and Gihobyeo were 90% and 0%, and 71 lines among 164 M/G RILs showed the germinability over 90%. The relationship between seed longevity and dry-heat tolerance was linearly correlated with high positive correlation coefficient. The relationships between seed longevity and viviparous germination, and viviparous germination and dry-heat tolerance were linearly correlated with high negative correlation coefficient. Milyang 23 had higher tolerance against high dry-heat, viviparous germination and long-term storage than Gihobyeo, and 164 M/G RILs showed the medium values of parents.

Factors affecting the vertical distribution of Betula platyphylla var. japonica and Betula ermanii on Mt. Neko in Nagano Prefecture, Japan

  • Jiro, Otsubo;Shigeru, Mariko;Ichiroku, Hayashi
    • Journal of Ecology and Environment
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    • v.33 no.2
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    • pp.121-131
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    • 2010
  • Betula platyphylla var. japonica and Betula ermanii segregate vertically at an elevation of approximately 1,850 m on Mt. Neko in Nagano Prefecture, Japan. B. platyphylla var. japonica and B. ermanii were the dominant species below and above this altitude, at which the mean-annual and growing-season air temperatures were $4^{\circ}C$ and $14.1^{\circ}C$, respectively. Based on a modification of Kira's warmth index which employs cumulative temperature represented as $^{\circ}C$ day, leaf unfolding in both species was observed to be initiated at $58^{\circ}C$ day and $169^{\circ}C$ day, respectively. In 1996, leaf unfolding was initiated on 18 May in B. platyphylla var. japonica (+/-6 days) and on 5 June in B. ermanii (+/-8 days), shortly after the last frost which occurred on 5 May 1995 above 1,850 m; below this elevation there was no risk of frost at the time. At elevations above 1,850 m, the unfolded leaves of B. platyphylla were damaged by late frost, while B. ermanii escaped injury because the leaves were still protected by winter buds. The optimum temperature for seed germination in both B. platyphylla and B. ermanii was $30^{\circ}C$. Temperature alternation from 10 to $30^{\circ}C$ and moist storage of seeds at $4^{\circ}C$ (stratification) prior to incubation increased germination rates in both species. The seedlings of B. ermanii had a greater survival rates than those of B. platyphylla var. japonica when planted above 1,850 m. Comparisons of the timing of leaf unfolding and the latest frost at a site appeared to be the main factors affecting the vertical distribution of these species.

Germinability of Film-Coated Snap Bean Seed as Affected by Oxygen Diffusion Rate under Different Soil Moisture Contents

  • Kim, Seok-Hyeon;Alan G. Taylor
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.1
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    • pp.46-51
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    • 2004
  • The film coated snap bean (Phaseolus vulgaris) seeds with five different coating materials treated with 3% increase in seed weight were planted at sandy loam soil controlled moisture content of 18, 19, 20 and 21 %. The oxygen diffusion rate (ODR) was calculated from the different moisture content soil. The number of normal seedlings, seedling vigor, and seedling capability in field (seed vigor x dry matter weight) were observed at 9 days after planting and compared to the changes of ODR. The germination rate and ODR were sharply decreased simultaneously in the seeds planted at 21 % soil moisture content. Seedling emergence did not occur at all as the soil moisture content increased above 22 %. Hence this value should be considered as the threshold of soil moisture content for seedling emergence. An ODR value under 20% did not influence the percent emergence significantly. The certain difference observing in the emergence at the same ODR was not related clearly to the condition of soil. So it can be assumed that the limit of soil moisture content for the emergence of snap bean was approximately 20%. The value of 18% soil moisture content may be considered as the optimum for snap bean emergence. There was close relationship between the mean value of ODR in different soil moisture contents and the emergence. The germination rates of the seeds coated with the different materials were quite different when the seeds were planted at 21 % soil moisture. Dry weight of the seedlings from film coated seeds was decreased slightly, but the germination rates were not much different from the non-treated control under relatively higher soil moisture content (21 %). Major factor lowering emergence rate was oxygen stress while film coating act as a minor constraint for snap bean sown in excess soil moisture condition.

Low Temperature Storage of Rough Rice Using Cold-Air in Winter(I) - Storage Characteristics after Rough Rice Cooling - (겨울철 냉기를 이용한 벼의 저온저장(I) - 벼 냉각 후 저장특성 -)

  • Lee J. S.;Han C. S.;Ham T. M.;Yon K. S.
    • Journal of Biosystems Engineering
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    • v.30 no.3 s.110
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    • pp.155-160
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    • 2005
  • The objective of this research was to establish a domestically available cooling storage technique by cold-air in winter, using winter cool air ventilation fur determining rough rice cooling method in the storage and dry bin. The rough rice storage characteristics of two test conditions, winter cool-air ventilation storage and ambient temperature storage, were evaluated from January to July 2001, using a storage and dry bin of 300-ton capacity. Results of this research are as follows: Grain temperature was from $-5.1\~-8.5^{\circ}C$ after winter cool-air ventilation, and grain initial temperature for ambient temperature bin storage was $0.3\~1.9^{\circ}C$. Moisture content of rough rice decreased from $0.28\;to\;0.93\%$ and from $1.53\;to\;1.92\%$ to compare with original moisture contents for winter cool-air ventilation, and for ambient temperature bin storage, respectively. Broken ratio of brown rice from winter cool-air ventilation bin increased from $0.16\;to\; 0.92\%$, and brown rice broken ratio was from $2.24\;to\;2.86\%$ for ambient temperature bin storage to compare with initial broken ratio. Hardness of stored rice increased along storage period increase in alt storage methods, and cooling bin storage increased rice hardness of 0.271kgf: this increasing was lower then the other methods from 0.059 to 2.239kgf. Germination rates were decreased approximately 9.03, 3.14 and $3.20\%$ for upper, middle, and bottom of ventilating winter air bin, respectively, and germination rates of 2.70, 3.47 and $4.14\%$ were approximately decreased for upper, middle, and bottom parts of ambient temperature bin storage, respectively.

Population Dynamics of Arisaema robustum (넓은잎천남성 (Arisaema robustum) 개체군의 동태)

  • 민병미;유진숙
    • The Korean Journal of Ecology
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    • v.21 no.1
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    • pp.27-33
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    • 1998
  • Arisaema robustum, which has the ability to change sex, was studied in a temperate broadleaf forest of Sanseong-ri, Joongbu-myeon, Gwangju-gun, Kyonggi Province, Korea. \ulcornerThe study, carried out from 1993 to 1997, focused on population dynamics energy budget among organs, size distribution, mortality, the relationships between sex and size, seed production and germination rate. In terms of energy budget among the organs, the ratio of aboveground to belowground biomass was 36.6 : 63.4 in non-female plants, and 81.4 : 18.6 in female plants. Also, in female plants, the ration of leaf to sexual organ biomass was 39.5 : 41.9. Therefore, the belowground ratio of female plants was lower than that of non-female plants. Plants were classified into 8 levels relative to the amount of leaf area by $100cm^2$. The rates of the smallest and the largest classes were 49% and 1%, respectively, and population distribution by size was relatively stable. The mortality averaged 13.1% per year and decreased in inverse proportion to leaf size (6.6% in the smallest and 0.0% in the largest size classes). Leaf areas were $64.1{\pm}48.5cm^2$ in non-flowering plants, $232.1{\pm}123.9cm^2$ in males and $444.8{\pm}153.9cm^2$ in females. The increase rates of leaf area per year varied from 1.9% in plants changing from female tomale, to 152.4% in plants changing from non-flowering to female. But plants which remained female for 2 years showed a decrease of 34.7%. >From this result, it is thought that the female plants invest more energy to reproduction than to vegetative organs. The correlation coefficient (CC) value between plant size and the number of seeds produced (0.55) was larger than the CC value between plant size and total seed weight (0.73). That is, the larger the plant size, the heavier the seed produced. The germination rate increased along with seed weight, and it was 95% in plants which were over 60mg fresh weight/seed.

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The Effects of Optimal Germination of Somatic Embryos Induced from Mature Cotyledon Explants of Panax ginseng C. A. Meyer by Gibberellic Acid (인삼 체세포 배 발아를 위한 $GA_3$의 최적 조건)

  • Kim, Young-Chang;Park, Hong-Woo;Kim, Ok-Tae;Bang, Kyong-Hwan;Hyun, Dong-Yun;Cha, Seon-Woo;Kim, Dong-Hwi
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.4
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    • pp.238-242
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    • 2009
  • Somatic embryos on growth regulator-free medium can be produced directly from cotyledon explants of Panax ginseng C. A. Meyer. When the embryo developmental stage was torpedo and cotyledon, the germination rate of embryos was quite high on MS medium supplemented with gibberellic acid ($GA_3$). However, the percentage of plantlet formation at the cotyledon stage was higher than that at the torpedo stage. This result demonstrates that the embryo at the cotyledon stage was the most appropriate for increasing germination by $GA_3$. Embryos cultured on medium including four levels of $GA_3$ concentrations (3, 5, 10, or 20 mg/$\ell$) showed all quite high germination rates (87-91%). When the well-developed embryos were continuously cultured on media including high concentrations of $GA_3$ from 10 to 20 mg/$\ell$, the percentage of formation of normal plantlets was lower than that seen under low concentrations from 3 to 5 mg/$\ell$. This treatment of high concentrations resulted in shoots with abnormal shape. The optimal $GA_3$ treatment provides a basis for the efficient method obtaining healthy plantlets derived from ginseng somatic embryos.

Performance of Imported Sweet Corn Hybrids in Korea

  • Seo, Seo-Jung;Yun, Yun-Sang;Lee, Lee-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.4
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    • pp.305-310
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    • 2002
  • The performance of 7 sugary (su) and 12 shrunken-2 (sh2) sweet com hybrids which are commercially grown in the United States was tested in Korea. The 100-seed weight of su hybrids (16.5-23.6 g) was much heavier compared to that of sh2 hybrids (10.9-17.5 g). The germination rate of su and sh2 hybrids at $25^{\circ}C$ ranged 93.3-100% and 86.7-98.9%, the emergence rate of su and sh2 hybrids in cold test ranged 78.9-97.8% and 62.2-97.8%, and field emergence rate of su and sh2 hybrids ranged 74.4-100.0% and 79.9-98.2%, respectively. In su hybrids, there was a significantly positive correlation between germination rate at $25^{\circ}C$ and emergence rate in cold test or early growth. In contrast, in sh2 hybrids seed weight was positively correlated with early plant growth, while not with the germination rate at $25^{\circ}C$ or emergence rate in cold test and field. Most sh2 hybrids produced larger and more marketable ears compared to su hybrids although there were significant differences among the hybrids in the same genotype. At harvest (24 days after pollination) soluble solids content of su hybrids (24.3-27.1 Brix %) was much higher than that of sh2 hybrids (13.8-18.0 Brix %), while total sugars of sh2 hybrids (21.4-28.6% on the dry weight basis) was much higher compared to su hybrids (2.4-15.9%). Considering germination and emergence rates, marketable ear production, and total sugar content, 'GCB 70' and 'Sweet Satin' in su hybrids and 'Ice Queen', 'Aspen', 'Sweet Magic', 'Bandit', 'Xtrasweet 82', 'Aspen', and 'Cambella 90' in sh2 hybrids performed better than other hybrids.

Allelopathic Effect of Ganghwa mugwort (Artemisia spp.) on Seed Germination and Seedling Growth of Plants (강화약쑥 추출물이 종자발아 및 유식물 생장에 미치는 알레로파시 효과)

  • Lee, Joo-Hwa;Byeon, Ji-Hui;Lee, Jeong-Hoon;Park, Chun-Geon;Park, Chung-Berm;Cho, Joon-Hyeong
    • Korean Journal of Organic Agriculture
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    • v.20 no.4
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    • pp.589-605
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    • 2012
  • This study was conducted to identify allelopathic effect of Ganghwa domestic Artemisia spp., named Sajabalssuk and Ssajuarissuk, for various receptor plants including clover (Trifolium repens L.), alfalfa (Medicago sativa L.), lawn grass (Zoysia japonica Steud.), dandelion (Taraxacum platycarpum Dahlst.), and dahurianpatrinia (Patrinia scabiosaefolia Fisch. ex Trevir). Receptor plants were treated with the aqueous and essential oil extract of Artemisia plants. In consequence, their allelopathic effects were evaluated by measuring seed germination rates, seedling growth, and dry weights of the receptor plants. The seed germination and seedling growth of the receptor plants were inhibited by all treatments of both aqueous and essential oil extracts of the Artemisia plants, and, in addition, the inhibitory effects were increased according to the higher concentration. Among the donor plants, A. $sp.^*III$ showed most effective allelopathic effect. Comparing the alleopathic effect among the receptor plants, seed germination was most inhibited in lawn grass while inhibitory effect of seedling growth was comparatively higher in dandelion. Although inhibitory effects were comparatively lower, the allelopathic effects of Artemisia plants were identified in clover and alfalfa since the seedling growth of these plants were inhibited more than 70%. Thus, in result, Ganghwa domestic Artemisia spp. could be possibly used for weed control since natural products of the plants showed inhibitory effects on seed germination and seedling growth of various receptor plants.