Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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The Effects of Optimal Germination of Somatic Embryos Induced from Mature Cotyledon Explants of Panax ginseng C. A. Meyer by Gibberellic Acid

인삼 체세포 배 발아를 위한 $GA_3$의 최적 조건

  • Kim, Young-Chang (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Park, Hong-Woo (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Ok-Tae (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Bang, Kyong-Hwan (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Hyun, Dong-Yun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Cha, Seon-Woo (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Dong-Hwi (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 김영창 (충북 음성군 농촌진흥청, 국립원예특작과학원, 인삼특작부) ;
  • 박홍우 (충북 음성군 농촌진흥청, 국립원예특작과학원, 인삼특작부) ;
  • 김옥태 (충북 음성군 농촌진흥청, 국립원예특작과학원, 인삼특작부) ;
  • 방경환 (충북 음성군 농촌진흥청, 국립원예특작과학원, 인삼특작부) ;
  • 현동윤 (충북 음성군 농촌진흥청, 국립원예특작과학원, 인삼특작부) ;
  • 차선우 (충북 음성군 농촌진흥청, 국립원예특작과학원, 인삼특작부) ;
  • 김동휘 (충북 음성군 농촌진흥청, 국립원예특작과학원, 인삼특작부)
  • Published : 2009.08.30
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Abstract

Somatic embryos on growth regulator-free medium can be produced directly from cotyledon explants of Panax ginseng C. A. Meyer. When the embryo developmental stage was torpedo and cotyledon, the germination rate of embryos was quite high on MS medium supplemented with gibberellic acid ($GA_3$). However, the percentage of plantlet formation at the cotyledon stage was higher than that at the torpedo stage. This result demonstrates that the embryo at the cotyledon stage was the most appropriate for increasing germination by $GA_3$. Embryos cultured on medium including four levels of $GA_3$ concentrations (3, 5, 10, or 20 mg/$\ell$) showed all quite high germination rates (87-91%). When the well-developed embryos were continuously cultured on media including high concentrations of $GA_3$ from 10 to 20 mg/$\ell$, the percentage of formation of normal plantlets was lower than that seen under low concentrations from 3 to 5 mg/$\ell$. This treatment of high concentrations resulted in shoots with abnormal shape. The optimal $GA_3$ treatment provides a basis for the efficient method obtaining healthy plantlets derived from ginseng somatic embryos.

Keywords

References

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